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The map label for this gene is mhpD [H]

Identifier: 94314126

GI number: 94314126

Start: 1901709

End: 1902530

Strand: Direct

Name: mhpD [H]

Synonym: Rmet_5207

Alternate gene names: 94314126

Gene position: 1901709-1902530 (Clockwise)

Preceding gene: 94314125

Following gene: 94314127

Centisome position: 73.71

GC content: 67.88

Gene sequence:

>822_bases
ATGCTGAACCCCGAACTCATCCAGGTTGCGGACCAACTCCGGTTCGCCTCCGAGCATGGCCAGCCTTGCCCGCCGGTGCG
CGATGCCATCGCGCGGGCCGCCAGCGCCGGCGCCGATCCACTTGCGCTGGCCTACGCCGTCCAGCAGCGCAACACCGAGC
ACGCACTCGCCGCCGGCCGCCGGCTGGTTGGCCGCAAGATCGGCCTGACATCCGTCGCCGTACAACGGCAACTGGGCGTG
GACCAGCCAGATTTCGGCATGCTCTTTGCCGACATGGTGCGCCAGGACGGTGAAGAGATCGACTGGGGCGATGTCCTGCA
GCCCAAGGTCGAAACCGAGATCGCGCTGGTGCTGGAGCATGACCTGCCGCACGCCGCGCACACCGTCGCCGACGTGATCC
GGGCAACCGCCTTCGCGCTGCCCGCCATCGAGATCGTTGGCAGCCGGATCGCCAACTGGGATATCCGGCTGACGGACACC
ATTGCCGACAATGCCTCGTCCGGCCTCTATGTGCTGGGCACGCGGCCCGTTTCGCTCGCCGCGCTGGATCTCCCGCATTG
CGGCATGGTCATGGAACTGGCCGGCGAACCGGTCTCAGTGGGCAGCGGCGCGGCCTGTCTTGGCAATCCGTTGAATGCCG
CCGTCTGGCTCGCCAACACAATGAGCCGCGTTGGCGCACCGCTGCGCGCCGGCGACACCATCCTTACCGGCGCGCTCGGC
CCCATGGTCGCCGTGCAGCCGGGCAATGTCTTTACCGCCAGCATCGAGGGCCTGGGTAGCGTGACCGCGTCGTTCCAGGC
CGTCAGACAAGGAGACGCCTGA

Upstream 100 bases:

>100_bases
CTGTCCGGTATCGGCCGCGAGGGTGGACGCCATTCGCTCGACTTCTATTCCTCCCTGACCACCATCTGCGTCAATCCCTG
ACGCCATTGCGAGAGACCGC

Downstream 100 bases:

>100_bases
GATGAAACCCTGCAAGGTAGCCATCATCGGTTCCGGCAACATCGGCACCGACCTGATGATCAAGATCCTGCGCCACGGCA
AGCATGTCGAGATGGGCGCC

Product: 2-keto-4-pentenoate hydratase

Products: 2-oxopent-4-enoate; H2O

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MLNPELIQVADQLRFASEHGQPCPPVRDAIARAASAGADPLALAYAVQQRNTEHALAAGRRLVGRKIGLTSVAVQRQLGV
DQPDFGMLFADMVRQDGEEIDWGDVLQPKVETEIALVLEHDLPHAAHTVADVIRATAFALPAIEIVGSRIANWDIRLTDT
IADNASSGLYVLGTRPVSLAALDLPHCGMVMELAGEPVSVGSGAACLGNPLNAAVWLANTMSRVGAPLRAGDTILTGALG
PMVAVQPGNVFTASIEGLGSVTASFQAVRQGDA

Sequences:

>Translated_273_residues
MLNPELIQVADQLRFASEHGQPCPPVRDAIARAASAGADPLALAYAVQQRNTEHALAAGRRLVGRKIGLTSVAVQRQLGV
DQPDFGMLFADMVRQDGEEIDWGDVLQPKVETEIALVLEHDLPHAAHTVADVIRATAFALPAIEIVGSRIANWDIRLTDT
IADNASSGLYVLGTRPVSLAALDLPHCGMVMELAGEPVSVGSGAACLGNPLNAAVWLANTMSRVGAPLRAGDTILTGALG
PMVAVQPGNVFTASIEGLGSVTASFQAVRQGDA
>Mature_273_residues
MLNPELIQVADQLRFASEHGQPCPPVRDAIARAASAGADPLALAYAVQQRNTEHALAAGRRLVGRKIGLTSVAVQRQLGV
DQPDFGMLFADMVRQDGEEIDWGDVLQPKVETEIALVLEHDLPHAAHTVADVIRATAFALPAIEIVGSRIANWDIRLTDT
IADNASSGLYVLGTRPVSLAALDLPHCGMVMELAGEPVSVGSGAACLGNPLNAAVWLANTMSRVGAPLRAGDTILTGALG
PMVAVQPGNVFTASIEGLGSVTASFQAVRQGDA

Specific function: Converts the product of 2-hydroxy-6-oxo-2,4- heptadienoate hydrolase [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=267, Percent_Identity=53.9325842696629, Blast_Score=272, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.80

Molecular weight: Translated: 28490; Mature: 28490

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNPELIQVADQLRFASEHGQPCPPVRDAIARAASAGADPLALAYAVQQRNTEHALAAGR
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHH
RLVGRKIGLTSVAVQRQLGVDQPDFGMLFADMVRQDGEEIDWGDVLQPKVETEIALVLEH
HHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHCEEEEEEEC
DLPHAAHTVADVIRATAFALPAIEIVGSRIANWDIRLTDTIADNASSGLYVLGTRPVSLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCEEEEECCCEEEE
ALDLPHCGMVMELAGEPVSVGSGAACLGNPLNAAVWLANTMSRVGAPLRAGDTILTGALG
EECCCCCCHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCC
PMVAVQPGNVFTASIEGLGSVTASFQAVRQGDA
CEEEECCCCEEEEECCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNPELIQVADQLRFASEHGQPCPPVRDAIARAASAGADPLALAYAVQQRNTEHALAAGR
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHH
RLVGRKIGLTSVAVQRQLGVDQPDFGMLFADMVRQDGEEIDWGDVLQPKVETEIALVLEH
HHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHCEEEEEEEC
DLPHAAHTVADVIRATAFALPAIEIVGSRIANWDIRLTDTIADNASSGLYVLGTRPVSLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCEEEEECCCEEEE
ALDLPHCGMVMELAGEPVSVGSGAACLGNPLNAAVWLANTMSRVGAPLRAGDTILTGALG
EECCCCCCHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCC
PMVAVQPGNVFTASIEGLGSVTASFQAVRQGDA
CEEEECCCCEEEEECCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxy-2-oxopentanoate

Specific reaction: 4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670929 [H]