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The map label for this gene is alsR [H]

Identifier: 94314070

GI number: 94314070

Start: 1849605

End: 1850513

Strand: Direct

Name: alsR [H]

Synonym: Rmet_5151

Alternate gene names: 94314070

Gene position: 1849605-1850513 (Clockwise)

Preceding gene: 94314069

Following gene: 94314076

Centisome position: 71.69

GC content: 63.26

Gene sequence:

>909_bases
ATGGATCTACGTCGCATCAGGCACTTTGTCGTACTTGCCGAGACTCTGAACTTCCGGCGCGCCGCCGAGCGGCTGCATAT
CGCCCAGCCGGCCCTGACCGTCTCCATACAGAAACTCGAAGCAGAGCTGGACACCACCTTGTTCCTGCGCGATGCCAGAG
GCGTGGCGCTGACCGCCAGCGGGCGGGTCGCGTTGCTGGAGGCAAAGCGCATCCTGTTCCACGCTGGCCAGTTCAGCGAG
CGGGTACACGCAGCCGCCAGTGGTCTGGGTGGCACATTGCGCGTGGGATTCGTCGGCTCCACGACGTATCAGGTACTCCC
GCGACTCGTCTCGCGCTTCCGCGAGGAATATCCGGGCATCGAACTCGTACTGAAAGAGGCAACGTCGACCAGCATTCTTC
GGAGCCTGGAGGAGGAAGTCCTGGATGTCGGCCTCATCAGGACGCCATTGATGTCCAGGGGCACTGCCACGCTGATTTCC
CTGCAGGAAGACCGGTTCGTTGCCGCGCTGCCACGGCGCAATCCGCTGGCGGCAAAGCAACCGCTTACGCTCGCCGACCT
TGCGCAAGAGCGGTTCGTCATGTATGCCCAGACCGAAGCATCGGGCCTGCACGCCGCTGCGATGGCGGCATGTCAGACCG
CCGGATTCCTTCCCCGGATCACGCAGGAAGCCACGCAGATCCAGACGGTATTGGCACTGGTGGAAAGCGGCCTTGGCGTC
GCGTTGGTCCCGTCGATCATGCGTCGATTCGGCAGTGACAAGCTGGTCTATTCGACACTGGCCAACTATCCCAACACCAC
GAGCATCGGCATCGCCCTGGCCTTTTCCCCGCACGCCGATGGTCCGGCCATCAGGCATTTCCGCGAAGTGGCGATTGCGG
AATTTGCCGGCGACGCGCCGGCCGCGTAG

Upstream 100 bases:

>100_bases
TTGCGACGCACAGCATGTTTCAATAAGCTCCAGGTCTCAAGCGCCCTCCGGAGCCAGAACACGACATTGAAATGCGGCGC
TCTGTCACCCTTCCAGGAAT

Downstream 100 bases:

>100_bases
GCTTCGGCCCGGATCGAAAAAGGACCCTGACCGAAACCGTAGCGACTTCGCCTCGCCGGCAGCACCTCAGAGTTCGCGCG
CGATCAGTTCCTTCATGATC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Als operon regulatory protein [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MDLRRIRHFVVLAETLNFRRAAERLHIAQPALTVSIQKLEAELDTTLFLRDARGVALTASGRVALLEAKRILFHAGQFSE
RVHAAASGLGGTLRVGFVGSTTYQVLPRLVSRFREEYPGIELVLKEATSTSILRSLEEEVLDVGLIRTPLMSRGTATLIS
LQEDRFVAALPRRNPLAAKQPLTLADLAQERFVMYAQTEASGLHAAAMAACQTAGFLPRITQEATQIQTVLALVESGLGV
ALVPSIMRRFGSDKLVYSTLANYPNTTSIGIALAFSPHADGPAIRHFREVAIAEFAGDAPAA

Sequences:

>Translated_302_residues
MDLRRIRHFVVLAETLNFRRAAERLHIAQPALTVSIQKLEAELDTTLFLRDARGVALTASGRVALLEAKRILFHAGQFSE
RVHAAASGLGGTLRVGFVGSTTYQVLPRLVSRFREEYPGIELVLKEATSTSILRSLEEEVLDVGLIRTPLMSRGTATLIS
LQEDRFVAALPRRNPLAAKQPLTLADLAQERFVMYAQTEASGLHAAAMAACQTAGFLPRITQEATQIQTVLALVESGLGV
ALVPSIMRRFGSDKLVYSTLANYPNTTSIGIALAFSPHADGPAIRHFREVAIAEFAGDAPAA
>Mature_302_residues
MDLRRIRHFVVLAETLNFRRAAERLHIAQPALTVSIQKLEAELDTTLFLRDARGVALTASGRVALLEAKRILFHAGQFSE
RVHAAASGLGGTLRVGFVGSTTYQVLPRLVSRFREEYPGIELVLKEATSTSILRSLEEEVLDVGLIRTPLMSRGTATLIS
LQEDRFVAALPRRNPLAAKQPLTLADLAQERFVMYAQTEASGLHAAAMAACQTAGFLPRITQEATQIQTVLALVESGLGV
ALVPSIMRRFGSDKLVYSTLANYPNTTSIGIALAFSPHADGPAIRHFREVAIAEFAGDAPAA

Specific function: Regulates the expression of the alsSD operon for acetoin biosynthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=289, Percent_Identity=31.8339100346021, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1788887, Length=287, Percent_Identity=34.4947735191638, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI2367136, Length=244, Percent_Identity=32.3770491803279, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790399, Length=247, Percent_Identity=28.3400809716599, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI157672245, Length=242, Percent_Identity=30.9917355371901, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI145693105, Length=247, Percent_Identity=28.7449392712551, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788297, Length=245, Percent_Identity=28.1632653061224, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790208, Length=253, Percent_Identity=26.4822134387352, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787601, Length=142, Percent_Identity=30.9859154929577, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787530, Length=249, Percent_Identity=25.3012048192771, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788748, Length=244, Percent_Identity=24.5901639344262, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789639, Length=122, Percent_Identity=30.327868852459, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787806, Length=248, Percent_Identity=24.1935483870968, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI87081904, Length=213, Percent_Identity=25.8215962441315, Blast_Score=61, Evalue=8e-11,
Organism=Escherichia coli, GI1788296, Length=236, Percent_Identity=26.6949152542373, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32726; Mature: 32726

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRRIRHFVVLAETLNFRRAAERLHIAQPALTVSIQKLEAELDTTLFLRDARGVALTAS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEECCCCEEEECC
GRVALLEAKRILFHAGQFSERVHAAASGLGGTLRVGFVGSTTYQVLPRLVSRFREEYPGI
CCEEEEHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCC
ELVLKEATSTSILRSLEEEVLDVGLIRTPLMSRGTATLISLQEDRFVAALPRRNPLAAKQ
EEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEEEECCCCEEEECCCCCCCCCCC
PLTLADLAQERFVMYAQTEASGLHAAAMAACQTAGFLPRITQEATQIQTVLALVESGLGV
CCHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCH
ALVPSIMRRFGSDKLVYSTLANYPNTTSIGIALAFSPHADGPAIRHFREVAIAEFAGDAP
HHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCC
AA
CC
>Mature Secondary Structure
MDLRRIRHFVVLAETLNFRRAAERLHIAQPALTVSIQKLEAELDTTLFLRDARGVALTAS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEECCCCEEEECC
GRVALLEAKRILFHAGQFSERVHAAASGLGGTLRVGFVGSTTYQVLPRLVSRFREEYPGI
CCEEEEHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCC
ELVLKEATSTSILRSLEEEVLDVGLIRTPLMSRGTATLISLQEDRFVAALPRRNPLAAKQ
EEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEEEECCCCEEEECCCCCCCCCCC
PLTLADLAQERFVMYAQTEASGLHAAAMAACQTAGFLPRITQEATQIQTVLALVESGLGV
CCHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCH
ALVPSIMRRFGSDKLVYSTLANYPNTTSIGIALAFSPHADGPAIRHFREVAIAEFAGDAP
HHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377 [H]