BacMap

Definition	Cupriavidus metallidurans CH34 megaplasmid, complete sequence.
Accession	NC_007974
Length	2,580,084

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The map label for this gene is crt [H]

Identifier: 94314064

GI number: 94314064

Start: 1843852

End: 1844634

Strand: Reverse

Name: crt [H]

Synonym: Rmet_5145

Alternate gene names: 94314064

Gene position: 1844634-1843852 (Counterclockwise)

Preceding gene: 94314065

Following gene: 94314063

Centisome position: 71.5

GC content: 64.5

Gene sequence:

>783_bases
ATGACGGTACTGGAAGTCAGGCACGTTGGAACCACCGCCGTGGTGACGATGAATCGTCCGGAGCAGCGCAACGCGCTCGA
CATGACGATGCGGGACGCATTCGCCGAGGTCGTTCCGGCGTTGCGCGATGACAAGGCGGTAAAGGCCATCGTGCTGACCG
GCGCTGGCGGGCACTTCTGCGCGGGGGGCGATGTGAAGGCGATTGCGGCCGGGCAGGGCGGCGAACGCGACATCTTCGAG
GGACGCGAGCGCATTCGCAAGATTCATCGCTGGTCCGACGAGTTGATCGACATGGAGAAGCCGGTCATCGCGGCCGTGGA
CGGCGTGGCGTTTGGCGCGGGACTCTCGCTGGCGCTTTGCGCGGACTTTATCCTGGCATCGAAACGGGCCACGTTCTGTT
CGGTGTTCTCCCGCATCGGATTCGTGCCCGATGTCGGGGCGTTATATCTGCTGCCTCGCTGGATCGGCCTCGCGCGCGCC
AAGGAACTGGTCTTCTCCGCGCGCGTCGTGAAGGCCGATGAGGCGCTCGACCTTGGGTTGCTCCATTCGATCTCCGATGA
CGATGTCGTGGCCGATGCCATTGCGCTGGCGAATCGCTTCGCGCACGCCCCGACCGACGCCATCGGCGCGGCGAAGTCCG
TGATGAACCGGGCGTTCGAGTCCGACCGTCATACCGTCCATGCGCAGGAAGCGATGCTGCAGGCCATGTGCCGCGAGAGC
GCCTATCACCAGGAGGCAGTCCGGAGGTTCATCGACAAGGAACCCGCGAAGTACCAGTGGTGA

Upstream 100 bases:

>100_bases
CTTTTGGCAAGTGGAAATGCAGGTGGAAAACAGCGAACGCCCCGCCATGGCGGCGACGTGGCTGCTGCAGATGCGCTACT
GAAAACAAAGGAGACAAGCA

Downstream 100 bases:

>100_bases
AGATCGCCACTGTGAACGTGATGTGAGGCACCGGTTGCCGGCGTGTTGCGGACACACGGCAGCACCGTGCCGGTTTTTTG
CACTGACGCCTGGCGGCAGT

Product: putative enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTVLEVRHVGTTAVVTMNRPEQRNALDMTMRDAFAEVVPALRDDKAVKAIVLTGAGGHFCAGGDVKAIAAGQGGERDIFE
GRERIRKIHRWSDELIDMEKPVIAAVDGVAFGAGLSLALCADFILASKRATFCSVFSRIGFVPDVGALYLLPRWIGLARA
KELVFSARVVKADEALDLGLLHSISDDDVVADAIALANRFAHAPTDAIGAAKSVMNRAFESDRHTVHAQEAMLQAMCRES
AYHQEAVRRFIDKEPAKYQW

Sequences:

>Translated_260_residues
MTVLEVRHVGTTAVVTMNRPEQRNALDMTMRDAFAEVVPALRDDKAVKAIVLTGAGGHFCAGGDVKAIAAGQGGERDIFE
GRERIRKIHRWSDELIDMEKPVIAAVDGVAFGAGLSLALCADFILASKRATFCSVFSRIGFVPDVGALYLLPRWIGLARA
KELVFSARVVKADEALDLGLLHSISDDDVVADAIALANRFAHAPTDAIGAAKSVMNRAFESDRHTVHAQEAMLQAMCRES
AYHQEAVRRFIDKEPAKYQW
>Mature_259_residues
TVLEVRHVGTTAVVTMNRPEQRNALDMTMRDAFAEVVPALRDDKAVKAIVLTGAGGHFCAGGDVKAIAAGQGGERDIFEG
RERIRKIHRWSDELIDMEKPVIAAVDGVAFGAGLSLALCADFILASKRATFCSVFSRIGFVPDVGALYLLPRWIGLARAK
ELVFSARVVKADEALDLGLLHSISDDDVVADAIALANRFAHAPTDAIGAAKSVMNRAFESDRHTVHAQEAMLQAMCRESA
YHQEAVRRFIDKEPAKYQW

Specific function: Catalyzes The Reversible Dehydration Of L-Carnitinyl-CoA To Crotobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=30.8, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI68989263, Length=209, Percent_Identity=32.0574162679426, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4502327, Length=266, Percent_Identity=31.9548872180451, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI260274832, Length=222, Percent_Identity=28.3783783783784, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI45643119, Length=222, Percent_Identity=28.3783783783784, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI260275230, Length=222, Percent_Identity=28.3783783783784, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI37594469, Length=151, Percent_Identity=32.4503311258278, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI37594471, Length=151, Percent_Identity=32.4503311258278, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI70995211, Length=186, Percent_Identity=32.7956989247312, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI25453481, Length=177, Percent_Identity=27.683615819209, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538573, Length=177, Percent_Identity=27.683615819209, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4757966, Length=177, Percent_Identity=27.683615819209, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538581, Length=177, Percent_Identity=27.683615819209, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4757968, Length=177, Percent_Identity=27.683615819209, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI49355787, Length=177, Percent_Identity=27.683615819209, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI20127408, Length=179, Percent_Identity=26.2569832402235, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI221307494, Length=176, Percent_Identity=27.8409090909091, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI221136756, Length=179, Percent_Identity=27.3743016759777, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI221136753, Length=179, Percent_Identity=27.3743016759777, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI31542718, Length=245, Percent_Identity=24.4897959183673, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI116235487, Length=178, Percent_Identity=28.0898876404494, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI221142681, Length=213, Percent_Identity=34.2723004694836, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787660, Length=247, Percent_Identity=34.412955465587, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=29.1187739463602, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788597, Length=186, Percent_Identity=32.258064516129, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=24.5136186770428, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=29.8507462686567, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790281, Length=179, Percent_Identity=25.1396648044693, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17540714, Length=249, Percent_Identity=28.1124497991968, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=256, Percent_Identity=28.515625, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=29.9595141700405, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=263, Percent_Identity=30.0380228136882, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17535521, Length=215, Percent_Identity=25.5813953488372, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=236, Percent_Identity=29.6610169491525, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=221, Percent_Identity=30.316742081448, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=248, Percent_Identity=29.0322580645161, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI25144160, Length=154, Percent_Identity=30.5194805194805, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI25144157, Length=154, Percent_Identity=30.5194805194805, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=271, Percent_Identity=24.3542435424354, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17558304, Length=227, Percent_Identity=25.1101321585903, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=194, Percent_Identity=28.3505154639175, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20129971, Length=263, Percent_Identity=28.5171102661597, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24653477, Length=263, Percent_Identity=28.5171102661597, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24653139, Length=256, Percent_Identity=29.296875, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21357171, Length=230, Percent_Identity=30.4347826086957, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24654903, Length=254, Percent_Identity=24.0157480314961, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19920382, Length=216, Percent_Identity=31.9444444444444, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571730, Length=231, Percent_Identity=26.8398268398268, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28571729, Length=231, Percent_Identity=26.8398268398268, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24650670, Length=259, Percent_Identity=23.9382239382239, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 28332; Mature: 28201

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLEVRHVGTTAVVTMNRPEQRNALDMTMRDAFAEVVPALRDDKAVKAIVLTGAGGHFC
CCEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEE
AGGDVKAIAAGQGGERDIFEGRERIRKIHRWSDELIDMEKPVIAAVDGVAFGAGLSLALC
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHCCHHHHHH
ADFILASKRATFCSVFSRIGFVPDVGALYLLPRWIGLARAKELVFSARVVKADEALDLGL
HHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LHSISDDDVVADAIALANRFAHAPTDAIGAAKSVMNRAFESDRHTVHAQEAMLQAMCRES
HHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
AYHQEAVRRFIDKEPAKYQW
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TVLEVRHVGTTAVVTMNRPEQRNALDMTMRDAFAEVVPALRDDKAVKAIVLTGAGGHFC
CEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEE
AGGDVKAIAAGQGGERDIFEGRERIRKIHRWSDELIDMEKPVIAAVDGVAFGAGLSLALC
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHCCHHHHHH
ADFILASKRATFCSVFSRIGFVPDVGALYLLPRWIGLARAKELVFSARVVKADEALDLGL
HHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LHSISDDDVVADAIALANRFAHAPTDAIGAAKSVMNRAFESDRHTVHAQEAMLQAMCRES
HHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
AYHQEAVRRFIDKEPAKYQW
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]