Click here to switch to the map view.

The map label for this gene is phaC3 [H]

Identifier: 94314041

GI number: 94314041

Start: 1816142

End: 1818052

Strand: Reverse

Name: phaC3 [H]

Synonym: Rmet_5122

Alternate gene names: 94314041

Gene position: 1818052-1816142 (Counterclockwise)

Preceding gene: 94314042

Following gene: 94314035

Centisome position: 70.46

GC content: 65.2

Gene sequence:

>1911_bases
ATGCGCCCCAAAAAGCCAGTCGCCCTCGAGCACACCACGGATACGCTGATCGAACAGGCCCCGCCCGCCAAACGGGCGAA
GGCCACCTCACCCAAGCCATCGACATCAACCCCTTCGGTTCCGGCTGCGCGGAAGACCGCCGCATCGGCACCGGCCAAAT
TGGGTCCGGCAGCTTCCCCCGCTCCGGCTGTGGCTACCTGCCCGGAACCGCCGCCGCTGCGGCCTGACGTCGCCTATGAC
GAACAGGTCAGGGCATTGCTCGCCTCGATGACCGGCGGCGTTTCCTTCGGGTCGCTGGCGCTAGCCTGGACCGACTGGGC
CATGCATCTGGCGGCCTCGCCCGGCAAACGCTGGCGGGCCGCGTTGCAGTGGCAGGCTACGGCCGCCGCCGAGTATTCGC
TGGCGCCGCCGTTTATGCCGCGTCCGCCGGTAGTCACCGACCAGCGCTTTTCCGCGCCGGGCTGGGAAAACTGGCCGTAC
TGCGTCTTGCGCGATGGCTTCCTGCGGCTTGAGTCCTTGTGGATGGCGCTGACCAGCGACATCGACGGGCTCTCCCCGCA
TCATCAGCGAATCGCGGCATTCTGCGCGCGCCAATGGCTCGACATGCTGTCTCCCAGCAATGTCTGGTGGATGAACCCGA
CGGTTCTGGATGCCACGATGCAGACGCGGGGCGCCAACTTCGTTCGCGGCGCCGGGAATCTCGTGGAGGATCTCTATGAT
GCGGTCGGCGAGCGGCTGCATCTGCCGATCGGGCGGCTGCCGCACGCGTATGTGGTGGGTCGCGATGTGGGGATCACGCC
GGGGAAGGTCGTCTACTGCAATGATCTGTTCGAGCTGATCCAGTACAGCCCGACTACCGATTCCGTGTATCGCGATCCGG
TCATGATCGTGCCGTCGTGGATCATGAAGTACTACATCCTCGATCTTCAGCCCCAGAACTCGATGGTGAAGTACCTCGTG
GACCAGGGTCATACGGTGTTCATGCTGTCATGGCGCAACCCGGGCGCCGAGGCGCGCGATACCAGCCTCGATGACTACCT
CAAGCAGGGCCTGCTTACCGCGCTGGCCACCGTGCGCGAGCGCTGCGGCCAAGAACAGGTACACGCCGTGGGGTATTGCC
TGGGCGGCACGCTGCTGGCGATTGCCGCCGCGAAGCTGGCGCGCGACAAGGACGACAGCCTGCGCAGCGTGACGCTATTT
GCAACGGAAACCGACTTCGACGAACCCGGCGAGCTTGGCCTGTTCATCGATGACAGCGCGTTGTCGACGCTCGATGCGCT
GATGCGCAAGCAGGGGTATCTCGACGGCTTCCAGATGGCGCAGGCCTTCTCGATGCTGCGTGCCAGGGATCTGGTCTGGT
CTCGGGTGCTGCGGGAGTACCTGCTGGGCGAGCGCGACCGCCCCAATGATCTGATGTCCTGGAACCGCGACACGACACGC
CTGCCGTATCGAATGCATAGCGAGGTGCTGTCCCGGCTCTTCCTCCGCAATGAACTGGCCGAAGGCCGGTTCTGCGTGGA
TGGTCACCCCGTGGCCATGGCCGATGTCGAGGCACCGATGTTCGTGGTTGGCACCGAGCACGATCACGTGGCGCCTTGGA
AATCCGCCTACAAGCTGCATCTGCTGACGCACAACGCGCTGACCTTCGTGCTGACGTCTGGCGGTCACAACGCGGGTGTT
GTTTCGGAGCCGGGGCACAAGGGTCGCCGCTACCGTGTGGCCACGCGGGTGCCGAACGGTGAGTACAAGGATCCGGATCG
TTATGCCGAAGAGGCCACCAGACATGACGGTTCATGGTGGCCGTGCTGGCACGAGTGGCTGGCCGAGCAGTCCTCGGGCC
GCTGCGCGCCACGCGATCCCGCGCCGGGTTCGCTCCGGGATGCGCCCGGCGAGTACGTCATGCAGGGCTGA

Upstream 100 bases:

>100_bases
GGAAGTGGCCGCGCTGATCAGTTACCTCTGCTCGGACGATGGCGCGTACGTGACCGGCGCCAACCTTGCCATCAATGGTG
GCCAACATATGGATTGATGC

Downstream 100 bases:

>100_bases
CTCCTGACTGCTGATCGCTGATCGCTATTGCTAGGTCCGTGCTCCGGTGGACGGCATCGTCACCGGAGCCTTTTTCATGT
TGCCGCTGACCATTCTGGTC

Product: poly-beta-hydroxybutyrate (PHB) polymerase / polyhydroxyalkanoic acid (PHA) synthase

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 636; Mature: 636

Protein sequence:

>636_residues
MRPKKPVALEHTTDTLIEQAPPAKRAKATSPKPSTSTPSVPAARKTAASAPAKLGPAASPAPAVATCPEPPPLRPDVAYD
EQVRALLASMTGGVSFGSLALAWTDWAMHLAASPGKRWRAALQWQATAAAEYSLAPPFMPRPPVVTDQRFSAPGWENWPY
CVLRDGFLRLESLWMALTSDIDGLSPHHQRIAAFCARQWLDMLSPSNVWWMNPTVLDATMQTRGANFVRGAGNLVEDLYD
AVGERLHLPIGRLPHAYVVGRDVGITPGKVVYCNDLFELIQYSPTTDSVYRDPVMIVPSWIMKYYILDLQPQNSMVKYLV
DQGHTVFMLSWRNPGAEARDTSLDDYLKQGLLTALATVRERCGQEQVHAVGYCLGGTLLAIAAAKLARDKDDSLRSVTLF
ATETDFDEPGELGLFIDDSALSTLDALMRKQGYLDGFQMAQAFSMLRARDLVWSRVLREYLLGERDRPNDLMSWNRDTTR
LPYRMHSEVLSRLFLRNELAEGRFCVDGHPVAMADVEAPMFVVGTEHDHVAPWKSAYKLHLLTHNALTFVLTSGGHNAGV
VSEPGHKGRRYRVATRVPNGEYKDPDRYAEEATRHDGSWWPCWHEWLAEQSSGRCAPRDPAPGSLRDAPGEYVMQG

Sequences:

>Translated_636_residues
MRPKKPVALEHTTDTLIEQAPPAKRAKATSPKPSTSTPSVPAARKTAASAPAKLGPAASPAPAVATCPEPPPLRPDVAYD
EQVRALLASMTGGVSFGSLALAWTDWAMHLAASPGKRWRAALQWQATAAAEYSLAPPFMPRPPVVTDQRFSAPGWENWPY
CVLRDGFLRLESLWMALTSDIDGLSPHHQRIAAFCARQWLDMLSPSNVWWMNPTVLDATMQTRGANFVRGAGNLVEDLYD
AVGERLHLPIGRLPHAYVVGRDVGITPGKVVYCNDLFELIQYSPTTDSVYRDPVMIVPSWIMKYYILDLQPQNSMVKYLV
DQGHTVFMLSWRNPGAEARDTSLDDYLKQGLLTALATVRERCGQEQVHAVGYCLGGTLLAIAAAKLARDKDDSLRSVTLF
ATETDFDEPGELGLFIDDSALSTLDALMRKQGYLDGFQMAQAFSMLRARDLVWSRVLREYLLGERDRPNDLMSWNRDTTR
LPYRMHSEVLSRLFLRNELAEGRFCVDGHPVAMADVEAPMFVVGTEHDHVAPWKSAYKLHLLTHNALTFVLTSGGHNAGV
VSEPGHKGRRYRVATRVPNGEYKDPDRYAEEATRHDGSWWPCWHEWLAEQSSGRCAPRDPAPGSLRDAPGEYVMQG
>Mature_636_residues
MRPKKPVALEHTTDTLIEQAPPAKRAKATSPKPSTSTPSVPAARKTAASAPAKLGPAASPAPAVATCPEPPPLRPDVAYD
EQVRALLASMTGGVSFGSLALAWTDWAMHLAASPGKRWRAALQWQATAAAEYSLAPPFMPRPPVVTDQRFSAPGWENWPY
CVLRDGFLRLESLWMALTSDIDGLSPHHQRIAAFCARQWLDMLSPSNVWWMNPTVLDATMQTRGANFVRGAGNLVEDLYD
AVGERLHLPIGRLPHAYVVGRDVGITPGKVVYCNDLFELIQYSPTTDSVYRDPVMIVPSWIMKYYILDLQPQNSMVKYLV
DQGHTVFMLSWRNPGAEARDTSLDDYLKQGLLTALATVRERCGQEQVHAVGYCLGGTLLAIAAAKLARDKDDSLRSVTLF
ATETDFDEPGELGLFIDDSALSTLDALMRKQGYLDGFQMAQAFSMLRARDLVWSRVLREYLLGERDRPNDLMSWNRDTTR
LPYRMHSEVLSRLFLRNELAEGRFCVDGHPVAMADVEAPMFVVGTEHDHVAPWKSAYKLHLLTHNALTFVLTSGGHNAGV
VSEPGHKGRRYRVATRVPNGEYKDPDRYAEEATRHDGSWWPCWHEWLAEQSSGRCAPRDPAPGSLRDAPGEYVMQG

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 70630; Mature: 70630

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPKKPVALEHTTDTLIEQAPPAKRAKATSPKPSTSTPSVPAARKTAASAPAKLGPAASP
CCCCCCEEEECCHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCC
APAVATCPEPPPLRPDVAYDEQVRALLASMTGGVSFGSLALAWTDWAMHLAASPGKRWRA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCHHHHH
ALQWQATAAAEYSLAPPFMPRPPVVTDQRFSAPGWENWPYCVLRDGFLRLESLWMALTSD
HHEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHC
IDGLSPHHQRIAAFCARQWLDMLSPSNVWWMNPTVLDATMQTRGANFVRGAGNLVEDLYD
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEHHHHHCCCCHHHCCHHHHHHHHH
AVGERLHLPIGRLPHAYVVGRDVGITPGKVVYCNDLFELIQYSPTTDSVYRDPVMIVPSW
HHHHEEECCCCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHCCCCCCCHHCCCEEEHHHH
IMKYYILDLQPQNSMVKYLVDQGHTVFMLSWRNPGAEARDTSLDDYLKQGLLTALATVRE
HHHHHHEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RCGQEQVHAVGYCLGGTLLAIAAAKLARDKDDSLRSVTLFATETDFDEPGELGLFIDDSA
HHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCEEEEECCHH
LSTLDALMRKQGYLDGFQMAQAFSMLRARDLVWSRVLREYLLGERDRPNDLMSWNRDTTR
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCC
LPYRMHSEVLSRLFLRNELAEGRFCVDGHPVAMADVEAPMFVVGTEHDHVAPWKSAYKLH
CCHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEE
LLTHNALTFVLTSGGHNAGVVSEPGHKGRRYRVATRVPNGEYKDPDRYAEEATRHDGSWW
EEECCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCC
PCWHEWLAEQSSGRCAPRDPAPGSLRDAPGEYVMQG
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRPKKPVALEHTTDTLIEQAPPAKRAKATSPKPSTSTPSVPAARKTAASAPAKLGPAASP
CCCCCCEEEECCHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCC
APAVATCPEPPPLRPDVAYDEQVRALLASMTGGVSFGSLALAWTDWAMHLAASPGKRWRA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCHHHHH
ALQWQATAAAEYSLAPPFMPRPPVVTDQRFSAPGWENWPYCVLRDGFLRLESLWMALTSD
HHEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHC
IDGLSPHHQRIAAFCARQWLDMLSPSNVWWMNPTVLDATMQTRGANFVRGAGNLVEDLYD
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEHHHHHCCCCHHHCCHHHHHHHHH
AVGERLHLPIGRLPHAYVVGRDVGITPGKVVYCNDLFELIQYSPTTDSVYRDPVMIVPSW
HHHHEEECCCCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHCCCCCCCHHCCCEEEHHHH
IMKYYILDLQPQNSMVKYLVDQGHTVFMLSWRNPGAEARDTSLDDYLKQGLLTALATVRE
HHHHHHEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RCGQEQVHAVGYCLGGTLLAIAAAKLARDKDDSLRSVTLFATETDFDEPGELGLFIDDSA
HHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCEEEEECCHH
LSTLDALMRKQGYLDGFQMAQAFSMLRARDLVWSRVLREYLLGERDRPNDLMSWNRDTTR
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCC
LPYRMHSEVLSRLFLRNELAEGRFCVDGHPVAMADVEAPMFVVGTEHDHVAPWKSAYKLH
CCHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEE
LLTHNALTFVLTSGGHNAGVVSEPGHKGRRYRVATRVPNGEYKDPDRYAEEATRHDGSWW
EEECCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCC
PCWHEWLAEQSSGRCAPRDPAPGSLRDAPGEYVMQG
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438 [H]