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The map label for this gene is fadA [C]

Identifier: 94314030

GI number: 94314030

Start: 1804613

End: 1805785

Strand: Reverse

Name: fadA [C]

Synonym: Rmet_5111

Alternate gene names: 94314030

Gene position: 1805785-1804613 (Counterclockwise)

Preceding gene: 94314031

Following gene: 94314029

Centisome position: 69.99

GC content: 65.13

Gene sequence:

>1173_bases
ATGAGCGCGGCATTGAAGGCCACCGATCGTGGTGGCGTGGCGATTCTGGGCACGGGACTGGCGGGGCTCGGTCATGCAGG
TGGCCGCACGGAGCAGGAAATCATCGCCCAGGCCGCGCACCGCGCGGTGGCGGCCAGCGGACTGCGCATGGCCGATATCG
ACGGCATTGTCACGTCGAGCCTGACGTCGCCCTGGTGGGTGATGCGCATGTCAGAGTACCTCGGCATCCGGCCCCGGTTC
TCCGATAGCACGATGTTCGGCGGATCGTCGTTCATCGCGGATGTTCGCATCGCCGCCATGGCGATCGAGGCGGGCGAATG
CGACAACGTGCTGATCTGCTACGGCAGCAATCCTCGCAGCATTCCCAGTAGTTCGCGCGTCAACGCGATGCGCGCCGAAC
TCGACCCGCAGCCGTACGAGCATCCGTACAAGCCGTTCAACCCCGTCACCAGCTATGCGCTGGCCGCGGCCCGGCATATG
CATGAGTTCGGCACCACGCGCACGCAACTGGCCGAAGTGGCGGTGGCGGCGCGGCAGTGGGCGCAGCGCAATCCCGACGC
CTTCATGCGCGACCCATTGACGGTCGACGATGTGATCAACGCCAAGATGGTGTCCGACCCGCTGACGGTGCGCGATTGCT
GTCTGGTGACCGATGGCGCCGGCGCTTTTGTCGTCACGCGCGCGGACCGTGCCCGGGGTCTTCATGCGAAGCCGGTCTAT
GTGCTCGGTGTCGGACACGCGCACTGGCATCGGCAGATTTCCTGCATGGACGATCTGACGCGCACGCCGGCCATCGACTC
GGGCCGCAAGGCATTTGCGCAGGCGGGCCTGACGCCTGCCGATATCGACGTGGTGGAGCTTTATGACGCTTTCACGATCA
ACCCGATCCTGTTTCTGGAAGACCTGGGCTTCTGCGCGAAAGGTGAGGGCGGTGCATTCATCTCGGGCGGCCGCATCGCG
CCGGGCGGCGATTTCCCGATGAACACCAATGGTGGCGGGCTGTCGTGCACCCACCCGGGCATGTACAGCATTTTCCTTGT
GATCGAAGCCGTCAGCCAGTTGCGCGGCGAGGCCGGCGAGCGGCAGGTCAGCAAGGTGGAAACGGCGCTAGTGCACGGCA
ATGGTGGTGTGCTGTCCAGCCAGGCCACCGCCATCCTGGCATCCAGCCAATAA

Upstream 100 bases:

>100_bases
TGGATCCCGACGCGGTGAGGATCGGGCACCGCGTCAGGGCGCAGGTGCAGCGCAAGGGCGAGGACGCATTGCTGGTGTTC
GCGCCGGCCGGGGAGCGACA

Downstream 100 bases:

>100_bases
AGGCCAAACCAGCCTAACAAAGAACCGCAGTCGAGATCGCAGCAATGTCCCAGGCAATCGAATCCATTCCTCTTTCTTCC
ACCGTGAGCTGGCAACAGCA

Product: thiolase

Products: CoA; oxoacyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSAALKATDRGGVAILGTGLAGLGHAGGRTEQEIIAQAAHRAVAASGLRMADIDGIVTSSLTSPWWVMRMSEYLGIRPRF
SDSTMFGGSSFIADVRIAAMAIEAGECDNVLICYGSNPRSIPSSSRVNAMRAELDPQPYEHPYKPFNPVTSYALAAARHM
HEFGTTRTQLAEVAVAARQWAQRNPDAFMRDPLTVDDVINAKMVSDPLTVRDCCLVTDGAGAFVVTRADRARGLHAKPVY
VLGVGHAHWHRQISCMDDLTRTPAIDSGRKAFAQAGLTPADIDVVELYDAFTINPILFLEDLGFCAKGEGGAFISGGRIA
PGGDFPMNTNGGGLSCTHPGMYSIFLVIEAVSQLRGEAGERQVSKVETALVHGNGGVLSSQATAILASSQ

Sequences:

>Translated_390_residues
MSAALKATDRGGVAILGTGLAGLGHAGGRTEQEIIAQAAHRAVAASGLRMADIDGIVTSSLTSPWWVMRMSEYLGIRPRF
SDSTMFGGSSFIADVRIAAMAIEAGECDNVLICYGSNPRSIPSSSRVNAMRAELDPQPYEHPYKPFNPVTSYALAAARHM
HEFGTTRTQLAEVAVAARQWAQRNPDAFMRDPLTVDDVINAKMVSDPLTVRDCCLVTDGAGAFVVTRADRARGLHAKPVY
VLGVGHAHWHRQISCMDDLTRTPAIDSGRKAFAQAGLTPADIDVVELYDAFTINPILFLEDLGFCAKGEGGAFISGGRIA
PGGDFPMNTNGGGLSCTHPGMYSIFLVIEAVSQLRGEAGERQVSKVETALVHGNGGVLSSQATAILASSQ
>Mature_389_residues
SAALKATDRGGVAILGTGLAGLGHAGGRTEQEIIAQAAHRAVAASGLRMADIDGIVTSSLTSPWWVMRMSEYLGIRPRFS
DSTMFGGSSFIADVRIAAMAIEAGECDNVLICYGSNPRSIPSSSRVNAMRAELDPQPYEHPYKPFNPVTSYALAAARHMH
EFGTTRTQLAEVAVAARQWAQRNPDAFMRDPLTVDDVINAKMVSDPLTVRDCCLVTDGAGAFVVTRADRARGLHAKPVYV
LGVGHAHWHRQISCMDDLTRTPAIDSGRKAFAQAGLTPADIDVVELYDAFTINPILFLEDLGFCAKGEGGAFISGGRIAP
GGDFPMNTNGGGLSCTHPGMYSIFLVIEAVSQLRGEAGERQVSKVETALVHGNGGVLSSQATAILASSQ

Specific function: Subunit Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI302344767, Length=268, Percent_Identity=31.3432835820896, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI19923233, Length=268, Percent_Identity=31.3432835820896, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI302344760, Length=268, Percent_Identity=31.3432835820896, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI302344762, Length=268, Percent_Identity=31.3432835820896, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI55956775, Length=231, Percent_Identity=29.4372294372294, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17537653, Length=235, Percent_Identity=27.6595744680851, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19921506, Length=229, Percent_Identity=32.3144104803493, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24585051, Length=232, Percent_Identity=31.8965517241379, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16 [C]

Molecular weight: Translated: 41334; Mature: 41203

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAALKATDRGGVAILGTGLAGLGHAGGRTEQEIIAQAAHRAVAASGLRMADIDGIVTSS
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCHHHHC
LTSPWWVMRMSEYLGIRPRFSDSTMFGGSSFIADVRIAAMAIEAGECDNVLICYGSNPRS
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHEEEEEEEECCCCCCEEEEECCCCCC
IPSSSRVNAMRAELDPQPYEHPYKPFNPVTSYALAAARHMHEFGTTRTQLAEVAVAARQW
CCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AQRNPDAFMRDPLTVDDVINAKMVSDPLTVRDCCLVTDGAGAFVVTRADRARGLHAKPVY
HHCCCCHHHCCCCCHHHHHCCHHHCCCCCEEEEEEEECCCCEEEEEECHHHCCCCCCCEE
VLGVGHAHWHRQISCMDDLTRTPAIDSGRKAFAQAGLTPADIDVVELYDAFTINPILFLE
EEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHEEHHHHHCCCCEEEEE
DLGFCAKGEGGAFISGGRIAPGGDFPMNTNGGGLSCTHPGMYSIFLVIEAVSQLRGEAGE
CCCCEECCCCCEEEECCEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCH
RQVSKVETALVHGNGGVLSSQATAILASSQ
HHHHHHHEEEEECCCCEECCCCEEEEECCC
>Mature Secondary Structure 
SAALKATDRGGVAILGTGLAGLGHAGGRTEQEIIAQAAHRAVAASGLRMADIDGIVTSS
CCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCHHHHC
LTSPWWVMRMSEYLGIRPRFSDSTMFGGSSFIADVRIAAMAIEAGECDNVLICYGSNPRS
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHEEEEEEEECCCCCCEEEEECCCCCC
IPSSSRVNAMRAELDPQPYEHPYKPFNPVTSYALAAARHMHEFGTTRTQLAEVAVAARQW
CCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AQRNPDAFMRDPLTVDDVINAKMVSDPLTVRDCCLVTDGAGAFVVTRADRARGLHAKPVY
HHCCCCHHHCCCCCHHHHHCCHHHCCCCCEEEEEEEECCCCEEEEEECHHHCCCCCCCEE
VLGVGHAHWHRQISCMDDLTRTPAIDSGRKAFAQAGLTPADIDVVELYDAFTINPILFLE
EEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHEEHHHHHCCCCEEEEE
DLGFCAKGEGGAFISGGRIAPGGDFPMNTNGGGLSCTHPGMYSIFLVIEAVSQLRGEAGE
CCCCEECCCCCEEEECCEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCH
RQVSKVETALVHGNGGVLSSQATAILASSQ
HHHHHHHEEEEECCCCEECCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acyl-CoA; acetyl-CoA [C]

Specific reaction: Acyl-CoA + acetyl-CoA = CoA + oxoacyl-CoA [C]

General reaction: Acyl group transfer; Claisen condensation [C]

Inhibitor: Tris (hydroxy methyl) aminomethane [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]