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The map label for this gene is shiA [H]

Identifier: 94314017

GI number: 94314017

Start: 1789793

End: 1791172

Strand: Direct

Name: shiA [H]

Synonym: Rmet_5098

Alternate gene names: 94314017

Gene position: 1789793-1791172 (Clockwise)

Preceding gene: 94314013

Following gene: 94314021

Centisome position: 69.37

GC content: 62.1

Gene sequence:

>1380_bases
ATGGCGAATCAACTCAGCGAGGCTGTCCCCGAAGCGAGTGTGGGACGACAACGCGGCACGACAACAACAAAAGATCTGCG
CCGCGTTGTCGCGGCTGCGATGGTTGGGTCCGTAGCGGAATGGTATGAGTTCTTCCTGTATGGAACGGCATCCGCACTGG
TATTCGGGACGCACTTCTTCAAGAAAACAGGGAATCCGGTCGATGGCCTGATCGCGGCCTTCGCGCTCTATGCCGTAGGG
TTCGCCGCACGGCCGATCGGCGGGATCGTCTTCGGTTACTACGGCGACAAGTTCGGCCGCAAGTACCTGCTCCAGGTCAG
TCTGGTAGCCGTGGGGATCACGACGTTCCTGATGGGCTGCCTTCCCACGTTCGACGTGATCGGGTACTGGGCCCCCGCCC
TGCTCGTCACGCTGCGCCTTATCCAGGGCTTTGCCTTCGGTGGCGAATGGGGTGGCGCCGTCCTTCTGGTGAGCGAGCAC
AGCCCGGACGACCGGCGCGGCTACTGGGCAAGCTGGCCGCAAGCAGGCGTGCCGGCTGGCAACCTCGTCGCCACGCTCAT
CCTGCTCGCGCTCTCGAGCACGCTGCCGGAACAGGACTTCCTGTCCTGGGGCTGGCGAGTCGCCTTCTGGTTCTCCGCCG
TGGTGGTGCTGATCGGGTTCTGGATCCGTCGCAAGGTCGATGATGCGCCGATCTTCAAGGAATCCCAGGAACGGCGCGAA
AAGGAACATGCGACCCAGCAAGGCGTCCGGTACGTTCTGCGCTATCACTGGAAGGAAGTGCTGATCGGCATTGGCGCGCG
CTTCGCGGAGAACATCCTCTACTACATGGTGGTGACGTTCTCCATCACGTACCTGAAGCTCGTGGTCGGCGCGGATACCA
CGCGCATTCTCAAGCTGATGTTCTTTGCCAATGCCCTGCACTTCATGATCATTCCGTTCATGGGCTACCTGTCGGACATC
ATCGGTCGCAAACCGGTGTACCTGACCGGCGCGGTCCTGACGGCGGCCTGGGGCTTCATTGGCTTCCCGATGATGGACAC
CGGGAACGACTGGATCATCATGGCTGCCATCGTGCTGGGCCTGGCAATCGAGTCGATGACCTACTCGCCCTACTCCGCGC
TCATGACCGAGATGTTCCCGACGAACGTCCGATACACCGCGCTCTCGCTGTGCTACCAGATCGCACCGCTGATGGCCGGA
TCGCTGGCTCCGCTGATCGCGCTGTCGCTGCTGGAGAAATACCACTCCTCCACGCCGATCGCCCTCTACCTGGTGGCCGC
CGCCGTGATCTCGATCATCAGCGTTGCCGCCGCGAAGGAAACGCGCGGCAAGTCATTGCGTGACGTCGACGCCGAGGCGA
AAGCCCGTGTGAATGGCTGA

Upstream 100 bases:

>100_bases
TTTCCGTATATTGCATGTTGTATAAAATCTCACCACTGACGCCGGTGTGGCGAGGCGGGAATCCACCCGCTGGTCAGCAG
AGACAAAAGGACGAGCAAAG

Downstream 100 bases:

>100_bases
TCGAAGTCTCCGAGCAACAAAAAAGCCGCCCTCTCCGGGCGGCTTTTGTTTTCGGGCCTGGCTATTGGCTCGATCCGCTC
TGCTTAGCTGGTGCCGCGCC

Product: putative transporter protein, MFS family

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MANQLSEAVPEASVGRQRGTTTTKDLRRVVAAAMVGSVAEWYEFFLYGTASALVFGTHFFKKTGNPVDGLIAAFALYAVG
FAARPIGGIVFGYYGDKFGRKYLLQVSLVAVGITTFLMGCLPTFDVIGYWAPALLVTLRLIQGFAFGGEWGGAVLLVSEH
SPDDRRGYWASWPQAGVPAGNLVATLILLALSSTLPEQDFLSWGWRVAFWFSAVVVLIGFWIRRKVDDAPIFKESQERRE
KEHATQQGVRYVLRYHWKEVLIGIGARFAENILYYMVVTFSITYLKLVVGADTTRILKLMFFANALHFMIIPFMGYLSDI
IGRKPVYLTGAVLTAAWGFIGFPMMDTGNDWIIMAAIVLGLAIESMTYSPYSALMTEMFPTNVRYTALSLCYQIAPLMAG
SLAPLIALSLLEKYHSSTPIALYLVAAAVISIISVAAAKETRGKSLRDVDAEAKARVNG

Sequences:

>Translated_459_residues
MANQLSEAVPEASVGRQRGTTTTKDLRRVVAAAMVGSVAEWYEFFLYGTASALVFGTHFFKKTGNPVDGLIAAFALYAVG
FAARPIGGIVFGYYGDKFGRKYLLQVSLVAVGITTFLMGCLPTFDVIGYWAPALLVTLRLIQGFAFGGEWGGAVLLVSEH
SPDDRRGYWASWPQAGVPAGNLVATLILLALSSTLPEQDFLSWGWRVAFWFSAVVVLIGFWIRRKVDDAPIFKESQERRE
KEHATQQGVRYVLRYHWKEVLIGIGARFAENILYYMVVTFSITYLKLVVGADTTRILKLMFFANALHFMIIPFMGYLSDI
IGRKPVYLTGAVLTAAWGFIGFPMMDTGNDWIIMAAIVLGLAIESMTYSPYSALMTEMFPTNVRYTALSLCYQIAPLMAG
SLAPLIALSLLEKYHSSTPIALYLVAAAVISIISVAAAKETRGKSLRDVDAEAKARVNG
>Mature_458_residues
ANQLSEAVPEASVGRQRGTTTTKDLRRVVAAAMVGSVAEWYEFFLYGTASALVFGTHFFKKTGNPVDGLIAAFALYAVGF
AARPIGGIVFGYYGDKFGRKYLLQVSLVAVGITTFLMGCLPTFDVIGYWAPALLVTLRLIQGFAFGGEWGGAVLLVSEHS
PDDRRGYWASWPQAGVPAGNLVATLILLALSSTLPEQDFLSWGWRVAFWFSAVVVLIGFWIRRKVDDAPIFKESQERREK
EHATQQGVRYVLRYHWKEVLIGIGARFAENILYYMVVTFSITYLKLVVGADTTRILKLMFFANALHFMIIPFMGYLSDII
GRKPVYLTGAVLTAAWGFIGFPMMDTGNDWIIMAAIVLGLAIESMTYSPYSALMTEMFPTNVRYTALSLCYQIAPLMAGS
LAPLIALSLLEKYHSSTPIALYLVAAAVISIISVAAAKETRGKSLRDVDAEAKARVNG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=428, Percent_Identity=35.0467289719626, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI1790550, Length=440, Percent_Identity=32.7272727272727, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1789941, Length=439, Percent_Identity=34.3963553530752, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1788942, Length=442, Percent_Identity=29.4117647058824, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI87082404, Length=420, Percent_Identity=23.3333333333333, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87082231, Length=400, Percent_Identity=20.25, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 50430; Mature: 50298

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHCCCC
>Mature Secondary Structure 
ANQLSEAVPEASVGRQRGTTTTKDLRRVVAAAMVGSVAEWYEFFLYGTASALVFGTHFF
CCCHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKTGNPVDGLIAAFALYAVGFAARPIGGIVFGYYGDKFGRKYLLQVSLVAVGITTFLMGC
HHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LPTFDVIGYWAPALLVTLRLIQGFAFGGEWGGAVLLVSEHSPDDRRGYWASWPQAGVPAG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCH
NLVATLILLALSSTLPEQDFLSWGWRVAFWFSAVVVLIGFWIRRKVDDAPIFKESQERRE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
KEHATQQGVRYVLRYHWKEVLIGIGARFAENILYYMVVTFSITYLKLVVGADTTRILKLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FFANALHFMIIPFMGYLSDIIGRKPVYLTGAVLTAAWGFIGFPMMDTGNDWIIMAAIVLG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
LAIESMTYSPYSALMTEMFPTNVRYTALSLCYQIAPLMAGSLAPLIALSLLEKYHSSTPI
HHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ALYLVAAAVISIISVAAAKETRGKSLRDVDAEAKARVNG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]