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The map label for this gene is hutC [H]

Identifier: 94313971

GI number: 94313971

Start: 1737489

End: 1738244

Strand: Reverse

Name: hutC [H]

Synonym: Rmet_5049

Alternate gene names: 94313971

Gene position: 1738244-1737489 (Counterclockwise)

Preceding gene: 94313972

Following gene: 94313970

Centisome position: 67.37

GC content: 64.81

Gene sequence:

>756_bases
ATGCCGCGTCAAACGCCTGAGCTGACGATGGACAGCAGTGCCGAGGACGCGCCAATGCCGCTCTATCTGCGCCTGAAGCA
GATGATCACCCGACAGATCGATACGGGCGCGTGGCCGGCGCATCATCGTGTGCCGTCCGAAAACGAACTGGTGGAGCAGT
TCGGTGCCAGCCGGATGACGATCAACCGCGCGTTGCGCGAACTCACCGCCGAAGGCCGGCTCGTGCGAATGCAGGGCGTG
GGCACGTTCGTGGCGGAGCCGAAGATGCAGTCTTCGCTGCTCGAGGTCAACAACATCGCGGACGAGATCGCGGAGCGCGG
ACATCGCCATCATTCCGAGGTGATCCTGGTGCGCGAGGAACCCGCCGGCCCGGAACGCGCGGTGGCGATGGATCTGCGCG
AAGGCGAGAAGATCTTCCATTCCATCATCGTTCACTACCAGGACAACGTGCCGGTGCAGATCGAGGATCGCTACGTCAAT
CCGGGTCTGGCTCCCGACTACCTGAAGCAGGACTTCAGCGTCGACACGCCGAATGCCTATCTGCAAAGCCTTGCTCCCCT
GACGGCGGGCGAGCATATCGTCGAGGCCGTTGTTGCTACCGCCGACGAGGCAAAGCTCCTGCAGATTCCGCGCGCCGAAC
CGTGCCTGCTGATCCGGCGCCGCACGTGGTCGGGCAAGCGCGTCGTGACGTTCGCCCGCCTGCTGCATCCGGGGTCGCGT
CACCGTCTGGAAGGGCGGTTCGGCCCCGGCGCCTGA

Upstream 100 bases:

>100_bases
GCATTTGCGATAATGCTGGCAATTGTCTAATCATGTATAGGTGCGCGAATCGGTCAACGCTTCGCGCTTTATCCTTGCCC
GGAGCTGGAGAACCCCAAGA

Downstream 100 bases:

>100_bases
CGCCCCCCTTCGTTTTTCCTTGCACCGCTGAGCCCCTGACCTTGCCGCGCCACTGTGCGGCAAGGTCCACTCCCGTCCCC
TTTGCCGCGCTATCTGCTCC

Product: histidine utilization repressor (transcriptional regulator, GntR family)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MPRQTPELTMDSSAEDAPMPLYLRLKQMITRQIDTGAWPAHHRVPSENELVEQFGASRMTINRALRELTAEGRLVRMQGV
GTFVAEPKMQSSLLEVNNIADEIAERGHRHHSEVILVREEPAGPERAVAMDLREGEKIFHSIIVHYQDNVPVQIEDRYVN
PGLAPDYLKQDFSVDTPNAYLQSLAPLTAGEHIVEAVVATADEAKLLQIPRAEPCLLIRRRTWSGKRVVTFARLLHPGSR
HRLEGRFGPGA

Sequences:

>Translated_251_residues
MPRQTPELTMDSSAEDAPMPLYLRLKQMITRQIDTGAWPAHHRVPSENELVEQFGASRMTINRALRELTAEGRLVRMQGV
GTFVAEPKMQSSLLEVNNIADEIAERGHRHHSEVILVREEPAGPERAVAMDLREGEKIFHSIIVHYQDNVPVQIEDRYVN
PGLAPDYLKQDFSVDTPNAYLQSLAPLTAGEHIVEAVVATADEAKLLQIPRAEPCLLIRRRTWSGKRVVTFARLLHPGSR
HRLEGRFGPGA
>Mature_250_residues
PRQTPELTMDSSAEDAPMPLYLRLKQMITRQIDTGAWPAHHRVPSENELVEQFGASRMTINRALRELTAEGRLVRMQGVG
TFVAEPKMQSSLLEVNNIADEIAERGHRHHSEVILVREEPAGPERAVAMDLREGEKIFHSIIVHYQDNVPVQIEDRYVNP
GLAPDYLKQDFSVDTPNAYLQSLAPLTAGEHIVEAVVATADEAKLLQIPRAEPCLLIRRRTWSGKRVVTFARLLHPGSRH
RLEGRFGPGA

Specific function: Repressor which binds to the hutP region in the histidine utilization (hut) operon. It blocks the expression of all the hut genes in the absence of inducer [H]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082252, Length=204, Percent_Identity=27.9411764705882, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786950, Length=199, Percent_Identity=24.6231155778894, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790118, Length=201, Percent_Identity=23.3830845771144, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010248
- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 28214; Mature: 28082

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRQTPELTMDSSAEDAPMPLYLRLKQMITRQIDTGAWPAHHRVPSENELVEQFGASRMT
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHH
INRALRELTAEGRLVRMQGVGTFVAEPKMQSSLLEVNNIADEIAERGHRHHSEVILVREE
HHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
PAGPERAVAMDLREGEKIFHSIIVHYQDNVPVQIEDRYVNPGLAPDYLKQDFSVDTPNAY
CCCCCHHEEHHHHHHHHHHHHHHHHCCCCCCEEEECEECCCCCCHHHHHHCCCCCCCHHH
LQSLAPLTAGEHIVEAVVATADEAKLLQIPRAEPCLLIRRRTWSGKRVVTFARLLHPGSR
HHHHCCCCCHHHHHHHHHHCCCCHHEEECCCCCCEEEEEECCCCCCHHHHHHHHHCCCCC
HRLEGRFGPGA
CCCCCCCCCCC
>Mature Secondary Structure 
PRQTPELTMDSSAEDAPMPLYLRLKQMITRQIDTGAWPAHHRVPSENELVEQFGASRMT
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHH
INRALRELTAEGRLVRMQGVGTFVAEPKMQSSLLEVNNIADEIAERGHRHHSEVILVREE
HHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
PAGPERAVAMDLREGEKIFHSIIVHYQDNVPVQIEDRYVNPGLAPDYLKQDFSVDTPNAY
CCCCCHHEEHHHHHHHHHHHHHHHHCCCCCCEEEECEECCCCCCHHHHHHCCCCCCCHHH
LQSLAPLTAGEHIVEAVVATADEAKLLQIPRAEPCLLIRRRTWSGKRVVTFARLLHPGSR
HHHHCCCCCHHHHHHHHHHCCCCHHEEECCCCCCEEEEEECCCCCCHHHHHHHHHCCCCC
HRLEGRFGPGA
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]