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The map label for this gene is ilvB [H]

Identifier: 94313961

GI number: 94313961

Start: 1725278

End: 1727113

Strand: Reverse

Name: ilvB [H]

Synonym: Rmet_5039

Alternate gene names: 94313961

Gene position: 1727113-1725278 (Counterclockwise)

Preceding gene: 94313963

Following gene: 94313960

Centisome position: 66.94

GC content: 65.58

Gene sequence:

>1836_bases
ATGCCCTTGTCTGCACCCGGCATCGGCAATTGCTGTCCGGATTCTTGCATACGATTAAGTATACTTAAAAGTATTCTTTC
GAGTATGCTCCATCGGACGCCGGCGCTTCCTTCACCCGGCCGATCAATGGAGATCAAGCGGATGACCAAGATGAACGGCG
CCGAAGCGATGGTGCGTATGCTCCAGCTCAACGGTGTGAAGCACATTTTTGGCTTGTGTGGCGATACCAGCCTGCCTTTT
TACGATGCTCTGGCCCGTCTGGACCATGGCATGGACCACGTGCTGGCGCGCGACGAGCGCAGCGCCGCGTACATGGCGGA
CGCCTACGCCCGCGTCACGGGCAAGGTCGGCGTGTGTGAGGGTCCGAGTGGAGGAGGCGCCACATATCTGTTGCCGGGGC
TGGTGGAGGCCAATGAATCGTCGGTGCCGGTGCTGGGCATTACGTCCGATGTATCCGTCAACTCGCGCGGCAAATACCCC
CTGACCGAGCTGGACCAGGAATCCCTGTATCGGCCACTGACCAAGTGGAACACCACGATCGACCGTGCCGATCAGATTCC
CGACGCCGTGCGCGCTGCCTTCCGCGCCATGACGACCGGCAAGCCGGGTTCGGCGCATCTGTGCCTCCCCTATGACGTTC
AGAAGCATGACGTGGACCCGGCCGGGATCTGGGCTCAGGCCGGTCACGATCGTTTCCCGGCGCTGCGCTACGCGCCGGAC
CCCGACGAGGTCGATCGCGCCGCGCGCCGTCTGACCGAAGCGCGCGCGCCGCTGATCATCTGCGGCGGTGGCGTGGTGAT
TTCGGGCGCCTGCGCTGAGCTGGACACGCTCGCGACCTCGCTCAACGCGCCGGTGTGCACCACGGTCAGCGGCCAGGGCA
GCCTGGCCGATACGCACCCGCTCAACGCCGGCGTGGTAGGCGCCAATGGCGGCATCCCGGCCACCCGCGATCTGGTGGCC
AATGCCGATGTCGTGCTGTTTATCGGTTGCCGCGCCGGCTCCACCACCACCGAGCACTGGCGCTTCCCCGGCCGCAACGT
GCCGATCCTGCATATCGACATCGATCCAATGGTGATCGCGGCCAACTACAACACCGACGTTGGTATGGTGGGCGATGCCT
TGCTGGCCCTTCGCATGTTGAACGCCGCAGTGCGTGACCGACTGCCGATGCGCCGTGCCGATACCGCCGACGGGCGCGCG
CTGGTGGAAGTCGCGCGCGCGGCCAAGCGTGCGAGCTTCGCACCGCTGGCGGCCTCGCTGGAGCGGCCGATCAAGCCCGA
GCGCGTGGTCGATACGCTCAACCGCCTCTTGCCCGAGGACGCCATCGTGGTGGCGGACCCTGGCACACCTTGCCCGTATT
TCTCCGCCTATCACGAGAGCCGACGCGCCGGCCGGCAGTTCATCACCAATCGCGCGCATGGTGCGCTGGGTTTCTCGCTG
GCGGCCGGTATCGGCGCTTCGCTGGGTCGCCCTGGCACCACGGTTGTCTCCGTGATGGGCGATGGCAGTTTCGGCTTTAC
CTGCGGCGAGATGGAAACGCTGGTACGCCGCCGCATCCCGCTGAAGATGATCGTGTTCTCGAACTCGGTGTTCGGCTGGA
TCAAGGCGAGCCAGAAGGCCGGCTACGACCGCCGCTACTTCTCCGTGGATTTCAGCCGCACCGATCACGCGCGCGTGGCC
GAGGCCTTTGGCGTGCGCGCGTGGCGCGTGGAAGATCCCGCGATGCTCGACGCAGCCATTCGCGCCGCGCTGGAGCATGA
CGGTCCCGCGCTGGTGGACGTCATCACGCAGGAGTTGCAGGATGCCGCGGCACCGGTCAGCCAGTGGATGGGCTGA

Upstream 100 bases:

>100_bases
GAACCGCGCTTGCGCCGAGTCGTGGAAATAGGCGAATTCATGCTTGTTTCTGCCCGATGGGATTCTTGAATCGCACGGAT
CATACCGCGCGCGCTACCAG

Downstream 100 bases:

>100_bases
GTCGATATCTGCTTTGCATCTGGATGACAGGAGGGCCGTACCATGCTGTTCCCTTGTTTCTCCTCGCGCCGGCAAACCAC
GCCGGCCGCTTCTGGCGGGA

Product: acetolactate synthase, large subunit (TPP dependent)

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 611; Mature: 610

Protein sequence:

>611_residues
MPLSAPGIGNCCPDSCIRLSILKSILSSMLHRTPALPSPGRSMEIKRMTKMNGAEAMVRMLQLNGVKHIFGLCGDTSLPF
YDALARLDHGMDHVLARDERSAAYMADAYARVTGKVGVCEGPSGGGATYLLPGLVEANESSVPVLGITSDVSVNSRGKYP
LTELDQESLYRPLTKWNTTIDRADQIPDAVRAAFRAMTTGKPGSAHLCLPYDVQKHDVDPAGIWAQAGHDRFPALRYAPD
PDEVDRAARRLTEARAPLIICGGGVVISGACAELDTLATSLNAPVCTTVSGQGSLADTHPLNAGVVGANGGIPATRDLVA
NADVVLFIGCRAGSTTTEHWRFPGRNVPILHIDIDPMVIAANYNTDVGMVGDALLALRMLNAAVRDRLPMRRADTADGRA
LVEVARAAKRASFAPLAASLERPIKPERVVDTLNRLLPEDAIVVADPGTPCPYFSAYHESRRAGRQFITNRAHGALGFSL
AAGIGASLGRPGTTVVSVMGDGSFGFTCGEMETLVRRRIPLKMIVFSNSVFGWIKASQKAGYDRRYFSVDFSRTDHARVA
EAFGVRAWRVEDPAMLDAAIRAALEHDGPALVDVITQELQDAAAPVSQWMG

Sequences:

>Translated_611_residues
MPLSAPGIGNCCPDSCIRLSILKSILSSMLHRTPALPSPGRSMEIKRMTKMNGAEAMVRMLQLNGVKHIFGLCGDTSLPF
YDALARLDHGMDHVLARDERSAAYMADAYARVTGKVGVCEGPSGGGATYLLPGLVEANESSVPVLGITSDVSVNSRGKYP
LTELDQESLYRPLTKWNTTIDRADQIPDAVRAAFRAMTTGKPGSAHLCLPYDVQKHDVDPAGIWAQAGHDRFPALRYAPD
PDEVDRAARRLTEARAPLIICGGGVVISGACAELDTLATSLNAPVCTTVSGQGSLADTHPLNAGVVGANGGIPATRDLVA
NADVVLFIGCRAGSTTTEHWRFPGRNVPILHIDIDPMVIAANYNTDVGMVGDALLALRMLNAAVRDRLPMRRADTADGRA
LVEVARAAKRASFAPLAASLERPIKPERVVDTLNRLLPEDAIVVADPGTPCPYFSAYHESRRAGRQFITNRAHGALGFSL
AAGIGASLGRPGTTVVSVMGDGSFGFTCGEMETLVRRRIPLKMIVFSNSVFGWIKASQKAGYDRRYFSVDFSRTDHARVA
EAFGVRAWRVEDPAMLDAAIRAALEHDGPALVDVITQELQDAAAPVSQWMG
>Mature_610_residues
PLSAPGIGNCCPDSCIRLSILKSILSSMLHRTPALPSPGRSMEIKRMTKMNGAEAMVRMLQLNGVKHIFGLCGDTSLPFY
DALARLDHGMDHVLARDERSAAYMADAYARVTGKVGVCEGPSGGGATYLLPGLVEANESSVPVLGITSDVSVNSRGKYPL
TELDQESLYRPLTKWNTTIDRADQIPDAVRAAFRAMTTGKPGSAHLCLPYDVQKHDVDPAGIWAQAGHDRFPALRYAPDP
DEVDRAARRLTEARAPLIICGGGVVISGACAELDTLATSLNAPVCTTVSGQGSLADTHPLNAGVVGANGGIPATRDLVAN
ADVVLFIGCRAGSTTTEHWRFPGRNVPILHIDIDPMVIAANYNTDVGMVGDALLALRMLNAAVRDRLPMRRADTADGRAL
VEVARAAKRASFAPLAASLERPIKPERVVDTLNRLLPEDAIVVADPGTPCPYFSAYHESRRAGRQFITNRAHGALGFSLA
AGIGASLGRPGTTVVSVMGDGSFGFTCGEMETLVRRRIPLKMIVFSNSVFGWIKASQKAGYDRRYFSVDFSRTDHARVAE
AFGVRAWRVEDPAMLDAAIRAALEHDGPALVDVITQELQDAAAPVSQWMG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=585, Percent_Identity=23.5897435897436, Blast_Score=143, Evalue=6e-34,
Organism=Homo sapiens, GI21361361, Length=566, Percent_Identity=25.2650176678445, Blast_Score=125, Evalue=8e-29,
Organism=Escherichia coli, GI87081685, Length=545, Percent_Identity=31.1926605504587, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1786717, Length=579, Percent_Identity=29.0155440414508, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1790104, Length=572, Percent_Identity=27.4475524475524, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1787096, Length=558, Percent_Identity=28.494623655914, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1788716, Length=495, Percent_Identity=25.0505050505051, Blast_Score=111, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17542570, Length=600, Percent_Identity=23.6666666666667, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17531299, Length=581, Percent_Identity=25.9896729776248, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17531301, Length=581, Percent_Identity=25.9896729776248, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=570, Percent_Identity=26.3157894736842, Blast_Score=197, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6320816, Length=495, Percent_Identity=22.6262626262626, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320123, Length=515, Percent_Identity=22.3300970873786, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19922626, Length=601, Percent_Identity=25.4575707154742, Blast_Score=166, Evalue=5e-41,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65316; Mature: 65185

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSAPGIGNCCPDSCIRLSILKSILSSMLHRTPALPSPGRSMEIKRMTKMNGAEAMVRM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHH
LQLNGVKHIFGLCGDTSLPFYDALARLDHGMDHVLARDERSAAYMADAYARVTGKVGVCE
HHHCCHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHCCCEEECC
GPSGGGATYLLPGLVEANESSVPVLGITSDVSVNSRGKYPLTELDQESLYRPLTKWNTTI
CCCCCCCEEEECCEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHH
DRADQIPDAVRAAFRAMTTGKPGSAHLCLPYDVQKHDVDPAGIWAQAGHDRFPALRYAPD
HHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHCCCCHHCCCCCCCCCCCCCCCCCCC
PDEVDRAARRLTEARAPLIICGGGVVISGACAELDTLATSLNAPVCTTVSGQGSLADTHP
HHHHHHHHHHHHHHCCCEEEECCCEEEECHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
LNAGVVGANGGIPATRDLVANADVVLFIGCRAGSTTTEHWRFPGRNVPILHIDIDPMVIA
CCCCEEECCCCCCCHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCCCCEEEEECCCEEEE
ANYNTDVGMVGDALLALRMLNAAVRDRLPMRRADTADGRALVEVARAAKRASFAPLAASL
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
ERPIKPERVVDTLNRLLPEDAIVVADPGTPCPYFSAYHESRRAGRQFITNRAHGALGFSL
HCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AAGIGASLGRPGTTVVSVMGDGSFGFTCGEMETLVRRRIPLKMIVFSNSVFGWIKASQKA
HHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCC
GYDRRYFSVDFSRTDHARVAEAFGVRAWRVEDPAMLDAAIRAALEHDGPALVDVITQELQ
CCCCEEEEEECCCCCHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DAAAPVSQWMG
HHHHHHHHHCC
>Mature Secondary Structure 
PLSAPGIGNCCPDSCIRLSILKSILSSMLHRTPALPSPGRSMEIKRMTKMNGAEAMVRM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHH
LQLNGVKHIFGLCGDTSLPFYDALARLDHGMDHVLARDERSAAYMADAYARVTGKVGVCE
HHHCCHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHCCCEEECC
GPSGGGATYLLPGLVEANESSVPVLGITSDVSVNSRGKYPLTELDQESLYRPLTKWNTTI
CCCCCCCEEEECCEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHH
DRADQIPDAVRAAFRAMTTGKPGSAHLCLPYDVQKHDVDPAGIWAQAGHDRFPALRYAPD
HHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHCCCCHHCCCCCCCCCCCCCCCCCCC
PDEVDRAARRLTEARAPLIICGGGVVISGACAELDTLATSLNAPVCTTVSGQGSLADTHP
HHHHHHHHHHHHHHCCCEEEECCCEEEECHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
LNAGVVGANGGIPATRDLVANADVVLFIGCRAGSTTTEHWRFPGRNVPILHIDIDPMVIA
CCCCEEECCCCCCCHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCCCCEEEEECCCEEEE
ANYNTDVGMVGDALLALRMLNAAVRDRLPMRRADTADGRALVEVARAAKRASFAPLAASL
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
ERPIKPERVVDTLNRLLPEDAIVVADPGTPCPYFSAYHESRRAGRQFITNRAHGALGFSL
HCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AAGIGASLGRPGTTVVSVMGDGSFGFTCGEMETLVRRRIPLKMIVFSNSVFGWIKASQKA
HHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCC
GYDRRYFSVDFSRTDHARVAEAFGVRAWRVEDPAMLDAAIRAALEHDGPALVDVITQELQ
CCCCEEEEEECCCCCHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DAAAPVSQWMG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9099862 [H]