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The map label for this gene is cusB [H]

Identifier: 94313954

GI number: 94313954

Start: 1717783

End: 1719300

Strand: Direct

Name: cusB [H]

Synonym: Rmet_5032

Alternate gene names: 94313954

Gene position: 1717783-1719300 (Clockwise)

Preceding gene: 94313953

Following gene: 94313955

Centisome position: 66.58

GC content: 64.95

Gene sequence:

>1518_bases
ATGAACGCACGACTGACCATCACGGCGACTTGCCTGGTCCTGATTGGCGTGGCCGGCTACAGCGGGTATCACCTTGGCGC
ATTGCACACCATGCCGGCAACGTCCGCCACGCAAGGCGACAGGAAAATTCTCTACTGGCACGACCCCATGGTCCCCGGCC
AGCGATTCGACAAACCCGGCAAGTCACCCTTCATGGATATGCCGCTGGTACCGGTCTATGCCGACGGCGATGACGCAGGG
AATGGCGTGAATAAGGGCGGCGTCAGCATCGACAGCCGCATCGCACAGAACCTTGGCATCCGCACGGCGGAAGTGAAATC
CGCCCATATGGGCGCAGTGGTTGAGGCACCCGGCAACGTGGCGATCGACGAGCGCAGCATCCGCGTGATCCAGGCTCGCA
CTACCGCCTTCGTGCAGCACGTCGGTGTGCGCGCCACCCTAGATCCGGTACGGCAGGGCCAGACGCTGGTCACTTTCTAC
GCGCCAGACTGGGTCGCCGCGCAAGAAGAGTACCTGGCGGTGATGCGCTTGCCCGTCAGCCTGCAGACGGATCTGGCGAG
CGCCGCCAAAGCGCGGATGCTGCAGTCGGGCATGACGCAGAGTCAGGCCGATGCGGTCATCCGCACCGGCAAGCTGCAAT
CGGCGCAGAGCATCGCCAGTCCGCTGGATGGCCTGGTCACCGAAATCGCGGTACGCGAAGGGATGACGATCAGCCCGGGC
ATGACGCTGTTCCGCCTGGCCGATCTCACTCAGGTCTGGGTGATTGCCGAAGTGCCGGAAGCCCAGGCGCGCAGCATCGC
ACCGGGCCAGACCGTGACGGTGTCTGCCACCGCCGCGCCGGATCCCGTGCAAGGCAAGGTCGACGCGATTCTGCCCGACG
TTGACCCTGCCACGCGCACCATCAAGGTCCGCATCGTGCTGCCGAACAAGCAGCGTCGCCTTGTGCCCGGCATGTTCGCC
AGCGTCCGCTTCGAAAACGGCGACCGGCAAGACGTGCTGCAGATCCCGACCGAGGCAGTGATCCGCACGGGGCAGCGGAG
TCTCGTCATGGTCGATGCCGGCCAGCGGGGTTATGTCTCTACCGAGATCCGCACCGGGCGCGAGGTCGACGGCATGACCG
AGGTGATCTCCGGACTGCGTGCCGGACAGCGCGTCGTAACGTCCGGCCAGTTCCTGATCGATTCCGAAGCCAGCCTGCGC
GGCACGTCGGAGCGGCTGGAAACCCCGGTCACAGCTTCTGCGCCGTCGGCACCATCGACACCATCGACGGCAACACAGGA
GCACGAAGCCACGGGTCGCATCGAAGCCGTGAACGCCGACAGCCTGACGATCTCCCATAGTGCAGTACCCTCGGCGCAGT
GGGGTGCCATGACGATGGATTTCGCCGCACATGATGCAGCCCAGCTCAAGGGACTGAAACCGGGCGATCGCATCCGCTTC
CGCTTTCATCTGGCCGAAGACGGCACGGCCATGCTGTCGTCGGTGCAGCCCGCAGGTGCCGAAGGAGGTCAGCGATGA

Upstream 100 bases:

>100_bases
GGCTATCGCTGGAACAACGCACGACCCGACTCTGGGCCGAACTCACCTTCCTGACACCGTTGCCTACCGCACAAACCGAA
CCCGCCAACAAGGATCTTCC

Downstream 100 bases:

>100_bases
TTGCACGCATCATCCTCGCGTCGATCCGCAACCGCTTCCTGGTGCTGCTGGCCACGATCATGCTGACGGCCTGGGGGCTA
TGGGCAGTCGGCACCACGCC

Product: copper and silver tricomponent efflux membrane fusion protein CusB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MNARLTITATCLVLIGVAGYSGYHLGALHTMPATSATQGDRKILYWHDPMVPGQRFDKPGKSPFMDMPLVPVYADGDDAG
NGVNKGGVSIDSRIAQNLGIRTAEVKSAHMGAVVEAPGNVAIDERSIRVIQARTTAFVQHVGVRATLDPVRQGQTLVTFY
APDWVAAQEEYLAVMRLPVSLQTDLASAAKARMLQSGMTQSQADAVIRTGKLQSAQSIASPLDGLVTEIAVREGMTISPG
MTLFRLADLTQVWVIAEVPEAQARSIAPGQTVTVSATAAPDPVQGKVDAILPDVDPATRTIKVRIVLPNKQRRLVPGMFA
SVRFENGDRQDVLQIPTEAVIRTGQRSLVMVDAGQRGYVSTEIRTGREVDGMTEVISGLRAGQRVVTSGQFLIDSEASLR
GTSERLETPVTASAPSAPSTPSTATQEHEATGRIEAVNADSLTISHSAVPSAQWGAMTMDFAAHDAAQLKGLKPGDRIRF
RFHLAEDGTAMLSSVQPAGAEGGQR

Sequences:

>Translated_505_residues
MNARLTITATCLVLIGVAGYSGYHLGALHTMPATSATQGDRKILYWHDPMVPGQRFDKPGKSPFMDMPLVPVYADGDDAG
NGVNKGGVSIDSRIAQNLGIRTAEVKSAHMGAVVEAPGNVAIDERSIRVIQARTTAFVQHVGVRATLDPVRQGQTLVTFY
APDWVAAQEEYLAVMRLPVSLQTDLASAAKARMLQSGMTQSQADAVIRTGKLQSAQSIASPLDGLVTEIAVREGMTISPG
MTLFRLADLTQVWVIAEVPEAQARSIAPGQTVTVSATAAPDPVQGKVDAILPDVDPATRTIKVRIVLPNKQRRLVPGMFA
SVRFENGDRQDVLQIPTEAVIRTGQRSLVMVDAGQRGYVSTEIRTGREVDGMTEVISGLRAGQRVVTSGQFLIDSEASLR
GTSERLETPVTASAPSAPSTPSTATQEHEATGRIEAVNADSLTISHSAVPSAQWGAMTMDFAAHDAAQLKGLKPGDRIRF
RFHLAEDGTAMLSSVQPAGAEGGQR
>Mature_505_residues
MNARLTITATCLVLIGVAGYSGYHLGALHTMPATSATQGDRKILYWHDPMVPGQRFDKPGKSPFMDMPLVPVYADGDDAG
NGVNKGGVSIDSRIAQNLGIRTAEVKSAHMGAVVEAPGNVAIDERSIRVIQARTTAFVQHVGVRATLDPVRQGQTLVTFY
APDWVAAQEEYLAVMRLPVSLQTDLASAAKARMLQSGMTQSQADAVIRTGKLQSAQSIASPLDGLVTEIAVREGMTISPG
MTLFRLADLTQVWVIAEVPEAQARSIAPGQTVTVSATAAPDPVQGKVDAILPDVDPATRTIKVRIVLPNKQRRLVPGMFA
SVRFENGDRQDVLQIPTEAVIRTGQRSLVMVDAGQRGYVSTEIRTGREVDGMTEVISGLRAGQRVVTSGQFLIDSEASLR
GTSERLETPVTASAPSAPSTPSTATQEHEATGRIEAVNADSLTISHSAVPSAQWGAMTMDFAAHDAAQLKGLKPGDRIRF
RFHLAEDGTAMLSSVQPAGAEGGQR

Specific function: Part of a cation efflux system that mediates resistance to copper and silver [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786787, Length=396, Percent_Identity=35.8585858585859, Blast_Score=233, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 53689; Mature: 53689

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARLTITATCLVLIGVAGYSGYHLGALHTMPATSATQGDRKILYWHDPMVPGQRFDKPG
CCCEEEEHHHHHHHHHHCCCCCEEECEEEECCCCCCCCCCCEEEEEECCCCCCHHCCCCC
KSPFMDMPLVPVYADGDDAGNGVNKGGVSIDSRIAQNLGIRTAEVKSAHMGAVVEAPGNV
CCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCEEEECCCCE
AIDERSIRVIQARTTAFVQHVGVRATLDPVRQGQTLVTFYAPDWVAAQEEYLAVMRLPVS
EECCCCEEEEEHHHHHHHHHCCCEEEHHHHHCCCEEEEEECCCCHHHHHHHHEEHCCCCH
LQTDLASAAKARMLQSGMTQSQADAVIRTGKLQSAQSIASPLDGLVTEIAVREGMTISPG
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCC
MTLFRLADLTQVWVIAEVPEAQARSIAPGQTVTVSATAAPDPVQGKVDAILPDVDPATRT
CHHHHHHHHEEEEEEEECCCHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCEE
IKVRIVLPNKQRRLVPGMFASVRFENGDRQDVLQIPTEAVIRTGQRSLVMVDAGQRGYVS
EEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHCCHHHHHHCCCCEEEEEECCCCCEEE
TEIRTGREVDGMTEVISGLRAGQRVVTSGQFLIDSEASLRGTSERLETPVTASAPSAPST
EECCCCCCCCCHHHHHHHHHCCCEEEECCCEEEECCCCCCCCHHHHCCCCCCCCCCCCCC
PSTATQEHEATGRIEAVNADSLTISHSAVPSAQWGAMTMDFAAHDAAQLKGLKPGDRIRF
CCCCCCCHHCCCCEEEECCCCEEEECCCCCCCCCCCEEEEHHHCCHHHHCCCCCCCEEEE
RFHLAEDGTAMLSSVQPAGAEGGQR
EEEECCCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNARLTITATCLVLIGVAGYSGYHLGALHTMPATSATQGDRKILYWHDPMVPGQRFDKPG
CCCEEEEHHHHHHHHHHCCCCCEEECEEEECCCCCCCCCCCEEEEEECCCCCCHHCCCCC
KSPFMDMPLVPVYADGDDAGNGVNKGGVSIDSRIAQNLGIRTAEVKSAHMGAVVEAPGNV
CCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCEEEECCCCE
AIDERSIRVIQARTTAFVQHVGVRATLDPVRQGQTLVTFYAPDWVAAQEEYLAVMRLPVS
EECCCCEEEEEHHHHHHHHHCCCEEEHHHHHCCCEEEEEECCCCHHHHHHHHEEHCCCCH
LQTDLASAAKARMLQSGMTQSQADAVIRTGKLQSAQSIASPLDGLVTEIAVREGMTISPG
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCC
MTLFRLADLTQVWVIAEVPEAQARSIAPGQTVTVSATAAPDPVQGKVDAILPDVDPATRT
CHHHHHHHHEEEEEEEECCCHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCEE
IKVRIVLPNKQRRLVPGMFASVRFENGDRQDVLQIPTEAVIRTGQRSLVMVDAGQRGYVS
EEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHCCHHHHHHCCCCEEEEEECCCCCEEE
TEIRTGREVDGMTEVISGLRAGQRVVTSGQFLIDSEASLRGTSERLETPVTASAPSAPST
EECCCCCCCCCHHHHHHHHHCCCEEEECCCEEEECCCCCCCCHHHHCCCCCCCCCCCCCC
PSTATQEHEATGRIEAVNADSLTISHSAVPSAQWGAMTMDFAAHDAAQLKGLKPGDRIRF
CCCCCCCHHCCCCEEEECCCCEEEECCCCCCCCCCCEEEEHHHCCHHHHCCCCCCCEEEE
RFHLAEDGTAMLSSVQPAGAEGGQR
EEEECCCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]