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The map label for this gene is ykoW [H]

Identifier: 94313884

GI number: 94313884

Start: 1630563

End: 1631459

Strand: Reverse

Name: ykoW [H]

Synonym: Rmet_4962

Alternate gene names: 94313884

Gene position: 1631459-1630563 (Counterclockwise)

Preceding gene: 94313885

Following gene: 94313883

Centisome position: 63.23

GC content: 61.76

Gene sequence:

>897_bases
ATGTCATGGATGCAAGAGATGCACGCCAGACCCCGCGAGTCTGGTAAATCCGAGTGTCCTCAAATGCAGGTCGCCGAGGA
CACCATGGAATCCTGGTGGGCGCCGGAATTGCTACAGGTGGAAGTGCTACGCAACGCGATAGATTGTGGCGAATTCGTGG
CTTGCTATCAGCCGCAGTTCGATCTGGATGGGGGGCATTTGCGCGGAGTAGAAGCGCTCGCGCGCTGGGCGCGTCCCGGC
TTCGGCATGATTCCGCCGGCCGCATTCCTGGGGGCGTTGCGTGAGCACGATCTGCTGTCCGAACTGACGATCGTGATGAT
CTGGCACGCAGCCGCGGCGGTGCGCCGTTGGGAGAAGGGCGGACTTGCGCTTCCGATCTCGATCAATGCGGAGTGGCGCT
GCCTGGAAACCGGCAGGCTGCACGTGGCGCTGCGATCCGCCTGCCAGCATTTCGGCATCTCCATGCGTAGCCTGCATGTC
GAAGTGTCCGAGCAAACGGAAGTCAATGACCTAATGACGGCGCGGGACGCCATTCGGGCGCTCAGGGCAGAAGGCGTGAG
CATCGCGCTGGACGACTTCGGGACGGGCACCACTTCATTGCAGCTGCTTCGCCATATCGAGTGTGACTCGATCAAGCTCG
ACCAGTCGCTGGTGAAGAACATTGGACGTGACCCGCGCGCCGCATCCCTGCTGGGCAGCGTTATCGGCATGGTGCGCGCA
ATGGGCCTCAAGGCCATCGCCGAAGGGGTGGAAAACTTCGATGACCTGGAGTGCCTGATCAGACTTGGCTGCCATCAGGC
TCAGGGATTTCTGCTGGGAGCGCCGATGCCGGAGGCCCGCCTGGCCCATCAGTTCTTTAAGCGCCAGGTATCGGCGCGCT
GGAAACCGGAAATGTGA

Upstream 100 bases:

>100_bases
AAGTTTCTCCGATTCATGGTGACGGCATTCGTTCAGGCGAGGTATCTGTGCTGTCGTGGATAGATTGGCAAGGGTAAAAC
GTTCGAGAGGTAATAGCAGC

Downstream 100 bases:

>100_bases
GGTTTTCGCCGGGTCACGAATAACTGATTCTCATGATTCGGATTTTCTCTATTTGAGCAGGAAACGTCGCTCAATGGGGT
TGATGGATTATCTACTGCAG

Product: putative diguanylate phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSWMQEMHARPRESGKSECPQMQVAEDTMESWWAPELLQVEVLRNAIDCGEFVACYQPQFDLDGGHLRGVEALARWARPG
FGMIPPAAFLGALREHDLLSELTIVMIWHAAAAVRRWEKGGLALPISINAEWRCLETGRLHVALRSACQHFGISMRSLHV
EVSEQTEVNDLMTARDAIRALRAEGVSIALDDFGTGTTSLQLLRHIECDSIKLDQSLVKNIGRDPRAASLLGSVIGMVRA
MGLKAIAEGVENFDDLECLIRLGCHQAQGFLLGAPMPEARLAHQFFKRQVSARWKPEM

Sequences:

>Translated_298_residues
MSWMQEMHARPRESGKSECPQMQVAEDTMESWWAPELLQVEVLRNAIDCGEFVACYQPQFDLDGGHLRGVEALARWARPG
FGMIPPAAFLGALREHDLLSELTIVMIWHAAAAVRRWEKGGLALPISINAEWRCLETGRLHVALRSACQHFGISMRSLHV
EVSEQTEVNDLMTARDAIRALRAEGVSIALDDFGTGTTSLQLLRHIECDSIKLDQSLVKNIGRDPRAASLLGSVIGMVRA
MGLKAIAEGVENFDDLECLIRLGCHQAQGFLLGAPMPEARLAHQFFKRQVSARWKPEM
>Mature_297_residues
SWMQEMHARPRESGKSECPQMQVAEDTMESWWAPELLQVEVLRNAIDCGEFVACYQPQFDLDGGHLRGVEALARWARPGF
GMIPPAAFLGALREHDLLSELTIVMIWHAAAAVRRWEKGGLALPISINAEWRCLETGRLHVALRSACQHFGISMRSLHVE
VSEQTEVNDLMTARDAIRALRAEGVSIALDDFGTGTTSLQLLRHIECDSIKLDQSLVKNIGRDPRAASLLGSVIGMVRAM
GLKAIAEGVENFDDLECLIRLGCHQAQGFLLGAPMPEARLAHQFFKRQVSARWKPEM

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=249, Percent_Identity=32.5301204819277, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI87081921, Length=239, Percent_Identity=30.5439330543933, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787055, Length=242, Percent_Identity=32.2314049586777, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87081845, Length=240, Percent_Identity=32.0833333333333, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87081743, Length=236, Percent_Identity=30.5084745762712, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1786507, Length=245, Percent_Identity=27.7551020408163, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788502, Length=259, Percent_Identity=25.8687258687259, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI226510982, Length=236, Percent_Identity=27.9661016949153, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081980, Length=240, Percent_Identity=29.5833333333333, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790496, Length=236, Percent_Identity=26.271186440678, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788849, Length=235, Percent_Identity=26.8085106382979, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082096, Length=249, Percent_Identity=27.3092369477912, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 33179; Mature: 33048

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWMQEMHARPRESGKSECPQMQVAEDTMESWWAPELLQVEVLRNAIDCGEFVACYQPQF
CCHHHHHHCCCCCCCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
DLDGGHLRGVEALARWARPGFGMIPPAAFLGALREHDLLSELTIVMIWHAAAAVRRWEKG
CCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLALPISINAEWRCLETGRLHVALRSACQHFGISMRSLHVEVSEQTEVNDLMTARDAIRA
CEEEEEEECCCEEEECCCCHHHHHHHHHHHHCCCHHHEEEEECCCHHHHHHHHHHHHHHH
LRAEGVSIALDDFGTGTTSLQLLRHIECDSIKLDQSLVKNIGRDPRAASLLGSVIGMVRA
HHHCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MGLKAIAEGVENFDDLECLIRLGCHQAQGFLLGAPMPEARLAHQFFKRQVSARWKPEM
HHHHHHHHHHCCHHHHHHHHHHCCHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SWMQEMHARPRESGKSECPQMQVAEDTMESWWAPELLQVEVLRNAIDCGEFVACYQPQF
CHHHHHHCCCCCCCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
DLDGGHLRGVEALARWARPGFGMIPPAAFLGALREHDLLSELTIVMIWHAAAAVRRWEKG
CCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLALPISINAEWRCLETGRLHVALRSACQHFGISMRSLHVEVSEQTEVNDLMTARDAIRA
CEEEEEEECCCEEEECCCCHHHHHHHHHHHHCCCHHHEEEEECCCHHHHHHHHHHHHHHH
LRAEGVSIALDDFGTGTTSLQLLRHIECDSIKLDQSLVKNIGRDPRAASLLGSVIGMVRA
HHHCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MGLKAIAEGVENFDDLECLIRLGCHQAQGFLLGAPMPEARLAHQFFKRQVSARWKPEM
HHHHHHHHHHCCHHHHHHHHHHCCHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]