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The map label for this gene is thcB [H]

Identifier: 94313854

GI number: 94313854

Start: 1595254

End: 1597596

Strand: Direct

Name: thcB [H]

Synonym: Rmet_4932

Alternate gene names: 94313854

Gene position: 1595254-1597596 (Clockwise)

Preceding gene: 94313853

Following gene: 94313856

Centisome position: 61.83

GC content: 65.22

Gene sequence:

>2343_bases
ATGCCGCAGACGAACGCACCCGCCAGCAGTGGAAGCTGCCCGATCGACCACAGCGCCTTGCGCGCGCCCAACGGTTGCCC
GATCAGCCACCAGGCGGCGGCGTTCGATCCCTTCGAGGACGGCTATCAGCAGGACCCGCCCGAGTACGTGCGTTGGTCGC
GCGCGCAGGAGCCGGTGTTCTACAGTCCGAAGCTCGGCTACTGGGTGGTGACGCGCTACGACGACATCAAGGCGATCTTT
CGCGACAACATCACCTTCAGCCCGTCAATTGCGCTCGAGAAGATCACGCCCACGGGTGAGGCGGCCAATGCGGTGCTGGC
CTCGTACGGCTACGCGATGAATCGCACGCTCGTCAACGAAGACGAGCCCGCGCACATGCCGCGCCGCCGCGCGTTGATGG
AGCCGTTCACGCCCGCCGAACTGGCCCATCACGAACCGATGGTGCGCAAGCTCACACGCGAATACGTCGACCGTTTCATC
GACACCGGCCGCGCGGATCTGGTGGACGAGATGCTCTGGGAGGTACCGCTGACGGTGGCGCTGCATTTCCTCGGCGTGCC
CGAGGAAGACATGGACCTGTTACGCCAGTACTCGATCGCCCATACGGTCAATACATGGGGCCGCCCGAAGCCCGAGGAGC
AGGTGGCCGTGGCCCACGCTGTGGGCAACTTCTGGCAACTGGCCGGCCGCATTCTCGACAAGATGCGCGAAGATCCGTCC
GGCCCGGGCTGGATGCAGTACGGCCTGCGCAAGCAGCGCGAGTTGCCCGAGGTGGTCACCGATTCGTACCTGCACTCGAT
GATGATGGCCGGCATCGTGGCCGCGCACGAGACCACGGCCAATGCATCGGCCAACGCGATCAAGCTGCTGCTGCAACATC
CCGATGTCTGGCGGGAGATCTGCGAAGACCCGGCATTGATTCCAAACGCGGTGGAAGAATGCCTGCGGCACAACGGCTCG
GTGGCCGCCTGGCGGCGCCTTGTCACGCGCGACACCGAAGTGGGCGGCATGTCGCTCGCGGCTGGCAGCAAATTGCTGAT
CGTCACGTCGTCGGCCAACCACGACGAACATCATTTCGCCGATGCCGATCTGTTCGACATCCATCGTGACAATGCCAGCG
ACCAGCTTACGTTCGGCTACGGCTCGCACCAGTGCATGGGAAAAAACCTCGCGCGCATGGAGATGCAGATCTTCCTGGAG
GAACTGACCAGCCGGCTGCCCCATATGCGTCTGGCCGGGCAGCGCTTCACCTACGTGCCGAACACCTCGTTCCGTGGCCC
GGAGCATCTCTGGGTCGAATGGGACCCGGCACGCAACCCCGAACGGACCGATCCGACGGTGCTGGCGCCACGTGACGCGG
TCCGCATCGGCGAACCGACTGGCGGCACCACCGGCCGCACGCTCATTGTCGAGCGCGTCGAGACGGCCGCGCAGGGTGTG
TCGCGGATCCGCCTGGTTTCGCCCGATGGTCGCGCACTACCGCGCTGGTCGCCGGGCTCGCATATCGACATCGAATGCGG
CCACACCGGCATCTCGCGCCAGTATTCGCTGTGCGGCGACCCCGCCGATACCAGCGCCTTCGAGATCGCGGTGCTGCGCG
AGCCCGAAAGCCGTGGTGGATCGGCGTGGATTCATGCCAGCCTGCGCGCAGGCGACAAGCTCAAGGTGCGCGGCCCGCGC
AATCACTTCCGGCTCGACGAGACCTGCCGTCGCGCGATCTTCATCGCCGGTGGCATTGGCGTGACTCCGGTCAGCGCCAT
GGCAAGGCGTGCGAAAGAACTGGGCGTCGACTACACCTTCCACTATTGCGGCCGCAGCCGTGCCTCCATGGCGATGATCG
ATGAACTGCGCGCCCTGCATGGTGATCGCGTGCGGATTCATGCCGCGGATGAAGGCCAGCGCGCCGATCTCGCGCAAGTG
CTCGGCGCACCCGATGCGAACACGCAGATCTACGCTTGTGGACCGGCCCGGATGATCGAGGCGCTGGAAGCCCTGTGCGC
GACATGGCCCGAGGATTCGCTGCGCGTCGAACACTTCAGTTCGAAACTTGGAACGCTCGATCCCTCCAGGGAACAGCCGT
TTGCGGTCGAACTGAAGGACTCGGGGCTGACGCTTGAAGTTCCTCCGGACCAGACGCTGCTCGCCACCCTGCGCGCCGCG
AACATCGACGTGCAAAGCGATTGCGAGGAAGGCCTGTGTGGATCGTGTGAAGTGCGCGTGCTGGCCGGCGAGATCGACCA
CCGCGACGTCGTGCTGACGCGCGGCGAGCGTGATGCGAACAACCGGATGATGGCCTGCTGCTCGCGAGCGGCGAAGGGCG
GAAAGATCGTGCTGGGGCTGTAA

Upstream 100 bases:

>100_bases
GACCTGCTGCGTGCCGAGAGCACGCGCTGGACCAAGCTGATTCACGATCTGAAGATCACGTTGGACTAATCTCGTTGGAC
TAATCTCGCTGGACTGAACC

Downstream 100 bases:

>100_bases
GCCGGCGCGAGGGCTTGATGCGCCAGCCCGTGGCGCATCAACCGGCAACAACGCCACTCAGCACGGCATCACGGATGCCG
TGCGAGTACACCCCGAATCG

Product: ferredoxin, cytochrome P450

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 780; Mature: 779

Protein sequence:

>780_residues
MPQTNAPASSGSCPIDHSALRAPNGCPISHQAAAFDPFEDGYQQDPPEYVRWSRAQEPVFYSPKLGYWVVTRYDDIKAIF
RDNITFSPSIALEKITPTGEAANAVLASYGYAMNRTLVNEDEPAHMPRRRALMEPFTPAELAHHEPMVRKLTREYVDRFI
DTGRADLVDEMLWEVPLTVALHFLGVPEEDMDLLRQYSIAHTVNTWGRPKPEEQVAVAHAVGNFWQLAGRILDKMREDPS
GPGWMQYGLRKQRELPEVVTDSYLHSMMMAGIVAAHETTANASANAIKLLLQHPDVWREICEDPALIPNAVEECLRHNGS
VAAWRRLVTRDTEVGGMSLAAGSKLLIVTSSANHDEHHFADADLFDIHRDNASDQLTFGYGSHQCMGKNLARMEMQIFLE
ELTSRLPHMRLAGQRFTYVPNTSFRGPEHLWVEWDPARNPERTDPTVLAPRDAVRIGEPTGGTTGRTLIVERVETAAQGV
SRIRLVSPDGRALPRWSPGSHIDIECGHTGISRQYSLCGDPADTSAFEIAVLREPESRGGSAWIHASLRAGDKLKVRGPR
NHFRLDETCRRAIFIAGGIGVTPVSAMARRAKELGVDYTFHYCGRSRASMAMIDELRALHGDRVRIHAADEGQRADLAQV
LGAPDANTQIYACGPARMIEALEALCATWPEDSLRVEHFSSKLGTLDPSREQPFAVELKDSGLTLEVPPDQTLLATLRAA
NIDVQSDCEEGLCGSCEVRVLAGEIDHRDVVLTRGERDANNRMMACCSRAAKGGKIVLGL

Sequences:

>Translated_780_residues
MPQTNAPASSGSCPIDHSALRAPNGCPISHQAAAFDPFEDGYQQDPPEYVRWSRAQEPVFYSPKLGYWVVTRYDDIKAIF
RDNITFSPSIALEKITPTGEAANAVLASYGYAMNRTLVNEDEPAHMPRRRALMEPFTPAELAHHEPMVRKLTREYVDRFI
DTGRADLVDEMLWEVPLTVALHFLGVPEEDMDLLRQYSIAHTVNTWGRPKPEEQVAVAHAVGNFWQLAGRILDKMREDPS
GPGWMQYGLRKQRELPEVVTDSYLHSMMMAGIVAAHETTANASANAIKLLLQHPDVWREICEDPALIPNAVEECLRHNGS
VAAWRRLVTRDTEVGGMSLAAGSKLLIVTSSANHDEHHFADADLFDIHRDNASDQLTFGYGSHQCMGKNLARMEMQIFLE
ELTSRLPHMRLAGQRFTYVPNTSFRGPEHLWVEWDPARNPERTDPTVLAPRDAVRIGEPTGGTTGRTLIVERVETAAQGV
SRIRLVSPDGRALPRWSPGSHIDIECGHTGISRQYSLCGDPADTSAFEIAVLREPESRGGSAWIHASLRAGDKLKVRGPR
NHFRLDETCRRAIFIAGGIGVTPVSAMARRAKELGVDYTFHYCGRSRASMAMIDELRALHGDRVRIHAADEGQRADLAQV
LGAPDANTQIYACGPARMIEALEALCATWPEDSLRVEHFSSKLGTLDPSREQPFAVELKDSGLTLEVPPDQTLLATLRAA
NIDVQSDCEEGLCGSCEVRVLAGEIDHRDVVLTRGERDANNRMMACCSRAAKGGKIVLGL
>Mature_779_residues
PQTNAPASSGSCPIDHSALRAPNGCPISHQAAAFDPFEDGYQQDPPEYVRWSRAQEPVFYSPKLGYWVVTRYDDIKAIFR
DNITFSPSIALEKITPTGEAANAVLASYGYAMNRTLVNEDEPAHMPRRRALMEPFTPAELAHHEPMVRKLTREYVDRFID
TGRADLVDEMLWEVPLTVALHFLGVPEEDMDLLRQYSIAHTVNTWGRPKPEEQVAVAHAVGNFWQLAGRILDKMREDPSG
PGWMQYGLRKQRELPEVVTDSYLHSMMMAGIVAAHETTANASANAIKLLLQHPDVWREICEDPALIPNAVEECLRHNGSV
AAWRRLVTRDTEVGGMSLAAGSKLLIVTSSANHDEHHFADADLFDIHRDNASDQLTFGYGSHQCMGKNLARMEMQIFLEE
LTSRLPHMRLAGQRFTYVPNTSFRGPEHLWVEWDPARNPERTDPTVLAPRDAVRIGEPTGGTTGRTLIVERVETAAQGVS
RIRLVSPDGRALPRWSPGSHIDIECGHTGISRQYSLCGDPADTSAFEIAVLREPESRGGSAWIHASLRAGDKLKVRGPRN
HFRLDETCRRAIFIAGGIGVTPVSAMARRAKELGVDYTFHYCGRSRASMAMIDELRALHGDRVRIHAADEGQRADLAQVL
GAPDANTQIYACGPARMIEALEALCATWPEDSLRVEHFSSKLGTLDPSREQPFAVELKDSGLTLEVPPDQTLLATLRAAN
IDVQSDCEEGLCGSCEVRVLAGEIDHRDVVLTRGERDANNRMMACCSRAAKGGKIVLGL

Specific function: Degradation of thiocarbamate herbicides [H]

COG id: COG2124

COG function: function code Q; Cytochrome P450

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome P450 family [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=319, Percent_Identity=37.9310344827586, Blast_Score=206, Evalue=5e-54,
Organism=Escherichia coli, GI1787098, Length=271, Percent_Identity=23.9852398523985, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001128
- InterPro:   IPR002397
- InterPro:   IPR017972 [H]

Pfam domain/function: PF00067 p450 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 86357; Mature: 86225

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00086 CYTOCHROME_P450 ; PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQTNAPASSGSCPIDHSALRAPNGCPISHQAAAFDPFEDGYQQDPPEYVRWSRAQEPVF
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHCCCCCCEE
YSPKLGYWVVTRYDDIKAIFRDNITFSPSIALEKITPTGEAANAVLASYGYAMNRTLVNE
ECCCCCEEEEEECHHHHHHHHCCCEECCCEEEEEECCCCHHHHHHHHHHCHHHCCCCCCC
DEPAHMPRRRALMEPFTPAELAHHEPMVRKLTREYVDRFIDTGRADLVDEMLWEVPLTVA
CCCCCCCHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHH
LHFLGVPEEDMDLLRQYSIAHTVNTWGRPKPEEQVAVAHAVGNFWQLAGRILDKMREDPS
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GPGWMQYGLRKQRELPEVVTDSYLHSMMMAGIVAAHETTANASANAIKLLLQHPDVWREI
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHH
CEDPALIPNAVEECLRHNGSVAAWRRLVTRDTEVGGMSLAAGSKLLIVTSSANHDEHHFA
HCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCCCCCHHCC
DADLFDIHRDNASDQLTFGYGSHQCMGKNLARMEMQIFLEELTSRLPHMRLAGQRFTYVP
CCCCEEEECCCCCCEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHCCCEEECCCEEEECC
NTSFRGPEHLWVEWDPARNPERTDPTVLAPRDAVRIGEPTGGTTGRTLIVERVETAAQGV
CCCCCCCCEEEEEECCCCCCCCCCCCEECCCCCEEECCCCCCCCCHHHHHHHHHHHHCCH
SRIRLVSPDGRALPRWSPGSHIDIECGHTGISRQYSLCGDPADTSAFEIAVLREPESRGG
HEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCHHCCCCCCCCCEEEEEEEECCCCCCC
SAWIHASLRAGDKLKVRGPRNHFRLDETCRRAIFIAGGIGVTPVSAMARRAKELGVDYTF
CEEEEEEECCCCEEEECCCCCCCCCCHHHCEEEEEECCCCCCHHHHHHHHHHHCCCCEEE
HYCGRSRASMAMIDELRALHGDRVRIHAADEGQRADLAQVLGAPDANTQIYACGPARMIE
EECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCEEEEECHHHHHH
ALEALCATWPEDSLRVEHFSSKLGTLDPSREQPFAVELKDSGLTLEVPPDQTLLATLRAA
HHHHHHHCCCCCCEEHHHHHHHHCCCCCCCCCCEEEEEECCCEEEECCCCHHHHHHHHHC
NIDVQSDCEEGLCGSCEVRVLAGEIDHRDVVLTRGERDANNRMMACCSRAAKGGKIVLGL
CCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
PQTNAPASSGSCPIDHSALRAPNGCPISHQAAAFDPFEDGYQQDPPEYVRWSRAQEPVF
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHCCCCCCEE
YSPKLGYWVVTRYDDIKAIFRDNITFSPSIALEKITPTGEAANAVLASYGYAMNRTLVNE
ECCCCCEEEEEECHHHHHHHHCCCEECCCEEEEEECCCCHHHHHHHHHHCHHHCCCCCCC
DEPAHMPRRRALMEPFTPAELAHHEPMVRKLTREYVDRFIDTGRADLVDEMLWEVPLTVA
CCCCCCCHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHH
LHFLGVPEEDMDLLRQYSIAHTVNTWGRPKPEEQVAVAHAVGNFWQLAGRILDKMREDPS
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GPGWMQYGLRKQRELPEVVTDSYLHSMMMAGIVAAHETTANASANAIKLLLQHPDVWREI
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHH
CEDPALIPNAVEECLRHNGSVAAWRRLVTRDTEVGGMSLAAGSKLLIVTSSANHDEHHFA
HCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCCCCCHHCC
DADLFDIHRDNASDQLTFGYGSHQCMGKNLARMEMQIFLEELTSRLPHMRLAGQRFTYVP
CCCCEEEECCCCCCEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHCCCEEECCCEEEECC
NTSFRGPEHLWVEWDPARNPERTDPTVLAPRDAVRIGEPTGGTTGRTLIVERVETAAQGV
CCCCCCCCEEEEEECCCCCCCCCCCCEECCCCCEEECCCCCCCCCHHHHHHHHHHHHCCH
SRIRLVSPDGRALPRWSPGSHIDIECGHTGISRQYSLCGDPADTSAFEIAVLREPESRGG
HEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCHHCCCCCCCCCEEEEEEEECCCCCCC
SAWIHASLRAGDKLKVRGPRNHFRLDETCRRAIFIAGGIGVTPVSAMARRAKELGVDYTF
CEEEEEEECCCCEEEECCCCCCCCCCHHHCEEEEEECCCCCCHHHHHHHHHHHCCCCEEE
HYCGRSRASMAMIDELRALHGDRVRIHAADEGQRADLAQVLGAPDANTQIYACGPARMIE
EECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCEEEEECHHHHHH
ALEALCATWPEDSLRVEHFSSKLGTLDPSREQPFAVELKDSGLTLEVPPDQTLLATLRAA
HHHHHHHCCCCCCEEHHHHHHHHCCCCCCCCCCEEEEEECCCEEEECCCCHHHHHHHHHC
NIDVQSDCEEGLCGSCEVRVLAGEIDHRDVVLTRGERDANNRMMACCSRAAKGGKIVLGL
CCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]