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The map label for this gene is xthA3 [H]

Identifier: 94313832

GI number: 94313832

Start: 1572151

End: 1573002

Strand: Reverse

Name: xthA3 [H]

Synonym: Rmet_4910

Alternate gene names: 94313832

Gene position: 1573002-1572151 (Counterclockwise)

Preceding gene: 94313833

Following gene: 94313827

Centisome position: 60.97

GC content: 62.68

Gene sequence:

>852_bases
ATGGGCCATCTCAAGATCGCGACGTTCAATATCAACGGCATCCACGCGCGGCTGCCGTCGCTGCTGGCATGGCTGGAGCG
CGAGCAGCCCGACGTAGCCTGTCTGCAGGAACTCAAGGCCGTGGACGAATCATTTCCGGTGGCAGAGCTCCACGCGGCAG
GCTACGGCGCAGTCTGGCAGGGGCAGAAATCATGGAATGGTGTGGCGATCCTCGCAAAAGGGTCGGATCCGGTCGAAGTG
CGGCGCGGTCTGCCAGGCGATGCTGCTGATACGCAGAGCCGCTATATCGAGGCGGCCGTTGCAGGCGTGCTGGTGGCGTG
CCTCTACCTGCCGAACGGCAATCCCCAGCCAGGGCCGAAGTTCGAATACAAGCTGGCTTGGTTCGAACGGTTGATTCGAC
ACGCAAGCACGCTCTACGCCAGCGGCCACCCGGTGGTACTGGCCGGCGATTTCAACGTGGTGCCGACCGACGACGACATC
TACAATCCGCGCTCGTGGCTCAAGGACGCACTGTTGCAGCCGGCGTCTCGCGAATGCTATCGACAGTTGCTTGAGCAGGG
ATGGACCGATGCGCTACGCACACGCTATCCCGACACCCGGATCTATACGTTCTGGGACTACTTTCGTCAGCACTGGCAGA
AGGACTCGGGTTTGCGCATCGATCATCTGCTGCTGAGCCGAAGTCTGGCGCAGCACATGCGTGACGCTGGCGTCGACAAA
TGGGTGCGTGGCGAGCCGCACGCGAGCGATCATGCGCCGGCGTGGGTGGAAATAGAAATGGGGGCGGAAGCGGAAGCGAG
AACGAAAACAACGCCCGCCGCGAGAGCAAGACGGCGGGCGTCGGGCGCGTGA

Upstream 100 bases:

>100_bases
CAAGGTTCATTGGGTTGACCCGGTTGTGGCCGCGAAATTGACGGGCGGAGCGTGAACCAGGCACCTAAGATGAATAGCTA
TCCGACCTGGAGCGGTGCCG

Downstream 100 bases:

>100_bases
TGCGCCGAAAACGGTAATGCCAGGCCTAGGCGGCTACGGCTTCGTGTTCCTCGATGGCGCACATCGAGCCGCCAACGGCG
CCAATCAGCATATTCGCTGC

Product: Exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MGHLKIATFNINGIHARLPSLLAWLEREQPDVACLQELKAVDESFPVAELHAAGYGAVWQGQKSWNGVAILAKGSDPVEV
RRGLPGDAADTQSRYIEAAVAGVLVACLYLPNGNPQPGPKFEYKLAWFERLIRHASTLYASGHPVVLAGDFNVVPTDDDI
YNPRSWLKDALLQPASRECYRQLLEQGWTDALRTRYPDTRIYTFWDYFRQHWQKDSGLRIDHLLLSRSLAQHMRDAGVDK
WVRGEPHASDHAPAWVEIEMGAEAEARTKTTPAARARRRASGA

Sequences:

>Translated_283_residues
MGHLKIATFNINGIHARLPSLLAWLEREQPDVACLQELKAVDESFPVAELHAAGYGAVWQGQKSWNGVAILAKGSDPVEV
RRGLPGDAADTQSRYIEAAVAGVLVACLYLPNGNPQPGPKFEYKLAWFERLIRHASTLYASGHPVVLAGDFNVVPTDDDI
YNPRSWLKDALLQPASRECYRQLLEQGWTDALRTRYPDTRIYTFWDYFRQHWQKDSGLRIDHLLLSRSLAQHMRDAGVDK
WVRGEPHASDHAPAWVEIEMGAEAEARTKTTPAARARRRASGA
>Mature_282_residues
GHLKIATFNINGIHARLPSLLAWLEREQPDVACLQELKAVDESFPVAELHAAGYGAVWQGQKSWNGVAILAKGSDPVEVR
RGLPGDAADTQSRYIEAAVAGVLVACLYLPNGNPQPGPKFEYKLAWFERLIRHASTLYASGHPVVLAGDFNVVPTDDDIY
NPRSWLKDALLQPASRECYRQLLEQGWTDALRTRYPDTRIYTFWDYFRQHWQKDSGLRIDHLLLSRSLAQHMRDAGVDKW
VRGEPHASDHAPAWVEIEMGAEAEARTKTTPAARARRRASGA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=258, Percent_Identity=31.7829457364341, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI18375503, Length=258, Percent_Identity=31.7829457364341, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI18375501, Length=258, Percent_Identity=31.7829457364341, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI1788046, Length=264, Percent_Identity=37.5, Blast_Score=160, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI71989536, Length=264, Percent_Identity=27.2727272727273, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI221330655, Length=260, Percent_Identity=30, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17136678, Length=260, Percent_Identity=30, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31703; Mature: 31572

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHLKIATFNINGIHARLPSLLAWLEREQPDVACLQELKAVDESFPVAELHAAGYGAVWQ
CCCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEC
GQKSWNGVAILAKGSDPVEVRRGLPGDAADTQSRYIEAAVAGVLVACLYLPNGNPQPGPK
CCCCCCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEYKLAWFERLIRHASTLYASGHPVVLAGDFNVVPTDDDIYNPRSWLKDALLQPASRECY
CCHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCCCHHHHHHHHHCCCCHHHHH
RQLLEQGWTDALRTRYPDTRIYTFWDYFRQHWQKDSGLRIDHLLLSRSLAQHMRDAGVDK
HHHHHHCCHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC
WVRGEPHASDHAPAWVEIEMGAEAEARTKTTPAARARRRASGA
CCCCCCCCCCCCCEEEEEEECCCCCHHCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
GHLKIATFNINGIHARLPSLLAWLEREQPDVACLQELKAVDESFPVAELHAAGYGAVWQ
CCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEC
GQKSWNGVAILAKGSDPVEVRRGLPGDAADTQSRYIEAAVAGVLVACLYLPNGNPQPGPK
CCCCCCCEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEYKLAWFERLIRHASTLYASGHPVVLAGDFNVVPTDDDIYNPRSWLKDALLQPASRECY
CCHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCCCHHHHHHHHHCCCCHHHHH
RQLLEQGWTDALRTRYPDTRIYTFWDYFRQHWQKDSGLRIDHLLLSRSLAQHMRDAGVDK
HHHHHHCCHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC
WVRGEPHASDHAPAWVEIEMGAEAEARTKTTPAARARRRASGA
CCCCCCCCCCCCCEEEEEEECCCCCHHCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]