| Definition | Cupriavidus metallidurans CH34 megaplasmid, complete sequence. |
|---|---|
| Accession | NC_007974 |
| Length | 2,580,084 |
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The map label for this gene is polS [H]
Identifier: 94313824
GI number: 94313824
Start: 1565363
End: 1566127
Strand: Reverse
Name: polS [H]
Synonym: Rmet_4902
Alternate gene names: 94313824
Gene position: 1566127-1565363 (Counterclockwise)
Preceding gene: 94313827
Following gene: 94313818
Centisome position: 60.7
GC content: 66.14
Gene sequence:
>765_bases ATGTACAAGCTCTTCGATATGTCCGGCCGCGTCGCGGTCGTCACGGGGTCCACCAAGGGAATGGGTCTGGAAATGGCGCG AGCGCTCGGCGCATCCGGGGCCCGGGTGGTGGTGTCTGGGCGCAACGCGGCGATCAGCGAAAAAGTTGCCGCCGCACTGA AGTCGGAGGGCATCGACGCCACTGGCCTCGCCTGCGACATCGCCGATACCGCCAGCGTGCGGGCCTTTGCCGACCGGGTG CTGGAGACGTTCGGCCGCGTGGACGCATTAGTCCTGAATGCCGCGGGCAGTGGTGTGGCCGGTTCAATCCTGGAGCAGGG GCCTGAGGACTTCGACGCGGTGATGGCGGGCAACGTGCGCGGCAATCTCGTGCTCGTCAACGCACTGGCGCCGCAGATGA TTTCCCGCAACGACGGCTCGATCATCTTCATGTCGAGCATCGCGGCACGTCGGGGATCCGCGCTGTTGCCGCTGTACAGC ATCTCGAAGGGGGCCGTCGATCTGGCGATGCGCAGCCTCGCTCATACGCTCGGACCGCACAACATCAATGTGAACAGCAT CAATCCGGGGCCGGTCCGCACCGATTTCTCGCGTGATGCGCTATGGGGCAACCCCGACCGCGAGAAGGCGTTGTCTGCCT CCATCCCGATGCGGCGCATTGCCGAGGCGCGCGACGTGGCGGGGTTGGCCGTGCTGCTGGCATCGCCCGCTGGACGCTAT ATCTCCGGGCAGACGATCGGGCTCGACGGCGGCGCTACGGCTTGA
Upstream 100 bases:
>100_bases AGTTTAGATGATGAACATACTTTATTCAATAGATGTCGAGCATCATCCAAATTGCAGGACCGACATCTACCTAACCTGAT CAGTTACAGGAGTTCCACTC
Downstream 100 bases:
>100_bases TCCAGGTACGGCGCACTGTATCGGCGTTTGGCGCACTTGGCGCACTTGGCGTCAGGTTCGGGTCAGTGCCACACCACCGG CGAGACGCTCCGACACGGCT
Product: short-chain dehydrogenase/reductase SDR
Products: NA
Alternate protein names: L-iditol 2-dehydrogenase; Polyol dehydrogenase [H]
Number of amino acids: Translated: 254; Mature: 254
Protein sequence:
>254_residues MYKLFDMSGRVAVVTGSTKGMGLEMARALGASGARVVVSGRNAAISEKVAAALKSEGIDATGLACDIADTASVRAFADRV LETFGRVDALVLNAAGSGVAGSILEQGPEDFDAVMAGNVRGNLVLVNALAPQMISRNDGSIIFMSSIAARRGSALLPLYS ISKGAVDLAMRSLAHTLGPHNINVNSINPGPVRTDFSRDALWGNPDREKALSASIPMRRIAEARDVAGLAVLLASPAGRY ISGQTIGLDGGATA
Sequences:
>Translated_254_residues MYKLFDMSGRVAVVTGSTKGMGLEMARALGASGARVVVSGRNAAISEKVAAALKSEGIDATGLACDIADTASVRAFADRV LETFGRVDALVLNAAGSGVAGSILEQGPEDFDAVMAGNVRGNLVLVNALAPQMISRNDGSIIFMSSIAARRGSALLPLYS ISKGAVDLAMRSLAHTLGPHNINVNSINPGPVRTDFSRDALWGNPDREKALSASIPMRRIAEARDVAGLAVLLASPAGRY ISGQTIGLDGGATA >Mature_254_residues MYKLFDMSGRVAVVTGSTKGMGLEMARALGASGARVVVSGRNAAISEKVAAALKSEGIDATGLACDIADTASVRAFADRV LETFGRVDALVLNAAGSGVAGSILEQGPEDFDAVMAGNVRGNLVLVNALAPQMISRNDGSIIFMSSIAARRGSALLPLYS ISKGAVDLAMRSLAHTLGPHNINVNSINPGPVRTDFSRDALWGNPDREKALSASIPMRRIAEARDVAGLAVLLASPAGRY ISGQTIGLDGGATA
Specific function: Catalyzes the oxidation of D-glucitol (sorbitol) to D- fructose, galactitol to D-tagatose and of L-iditol [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI32483357, Length=245, Percent_Identity=35.1020408163265, Blast_Score=151, Evalue=6e-37, Organism=Homo sapiens, GI5031737, Length=247, Percent_Identity=35.6275303643725, Blast_Score=148, Evalue=5e-36, Organism=Homo sapiens, GI126723750, Length=248, Percent_Identity=30.6451612903226, Blast_Score=128, Evalue=6e-30, Organism=Homo sapiens, GI33667109, Length=192, Percent_Identity=36.9791666666667, Blast_Score=119, Evalue=3e-27, Organism=Homo sapiens, GI40254992, Length=243, Percent_Identity=32.5102880658436, Blast_Score=105, Evalue=5e-23, Organism=Homo sapiens, GI7705925, Length=253, Percent_Identity=32.4110671936759, Blast_Score=104, Evalue=8e-23, Organism=Homo sapiens, GI126723191, Length=182, Percent_Identity=33.5164835164835, Blast_Score=97, Evalue=1e-20, Organism=Homo sapiens, GI20149619, Length=203, Percent_Identity=32.0197044334975, Blast_Score=97, Evalue=2e-20, Organism=Homo sapiens, GI10190704, Length=253, Percent_Identity=31.2252964426877, Blast_Score=92, Evalue=5e-19, Organism=Homo sapiens, GI66933014, Length=251, Percent_Identity=30.6772908366534, Blast_Score=90, Evalue=2e-18, Organism=Homo sapiens, GI15277342, Length=249, Percent_Identity=27.3092369477912, Blast_Score=86, Evalue=5e-17, Organism=Homo sapiens, GI19923817, Length=261, Percent_Identity=26.4367816091954, Blast_Score=85, Evalue=5e-17, Organism=Homo sapiens, GI59889578, Length=231, Percent_Identity=29.8701298701299, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI157739938, Length=193, Percent_Identity=28.4974093264249, Blast_Score=79, Evalue=5e-15, Organism=Homo sapiens, GI4503301, Length=249, Percent_Identity=26.5060240963855, Blast_Score=75, Evalue=4e-14, Organism=Homo sapiens, GI7706318, Length=205, Percent_Identity=29.7560975609756, Blast_Score=75, Evalue=6e-14, Organism=Homo sapiens, GI223718074, Length=207, Percent_Identity=30.9178743961353, Blast_Score=73, Evalue=3e-13, Organism=Homo sapiens, GI209862901, Length=203, Percent_Identity=29.064039408867, Blast_Score=73, Evalue=3e-13, Organism=Homo sapiens, GI19923983, Length=203, Percent_Identity=29.064039408867, Blast_Score=73, Evalue=3e-13, Organism=Homo sapiens, GI7657478, Length=191, Percent_Identity=31.413612565445, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI31542939, Length=199, Percent_Identity=33.1658291457286, Blast_Score=65, Evalue=6e-11, Organism=Homo sapiens, GI32455239, Length=183, Percent_Identity=29.5081967213115, Blast_Score=64, Evalue=9e-11, Organism=Homo sapiens, GI5031765, Length=183, Percent_Identity=29.5081967213115, Blast_Score=64, Evalue=9e-11, Organism=Escherichia coli, GI87082100, Length=256, Percent_Identity=35.9375, Blast_Score=120, Evalue=7e-29, Organism=Escherichia coli, GI1787905, Length=252, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=7e-29, Organism=Escherichia coli, GI1790717, Length=251, Percent_Identity=28.6852589641434, Blast_Score=114, Evalue=8e-27, Organism=Escherichia coli, GI87082160, Length=251, Percent_Identity=30.6772908366534, Blast_Score=111, Evalue=6e-26, Organism=Escherichia coli, GI1789208, Length=251, Percent_Identity=33.4661354581673, Blast_Score=110, Evalue=9e-26, Organism=Escherichia coli, GI1787335, Length=248, Percent_Identity=29.4354838709677, Blast_Score=100, Evalue=8e-23, Organism=Escherichia coli, GI1786812, Length=256, Percent_Identity=30.46875, Blast_Score=88, Evalue=6e-19, Organism=Escherichia coli, GI2367175, Length=246, Percent_Identity=30.4878048780488, Blast_Score=84, Evalue=1e-17, Organism=Escherichia coli, GI1787526, Length=191, Percent_Identity=31.9371727748691, Blast_Score=83, Evalue=2e-17, Organism=Escherichia coli, GI1788459, Length=245, Percent_Identity=26.9387755102041, Blast_Score=79, Evalue=3e-16, Organism=Escherichia coli, GI1789057, Length=255, Percent_Identity=30.5882352941176, Blast_Score=77, Evalue=2e-15, Organism=Escherichia coli, GI2367365, Length=245, Percent_Identity=29.3877551020408, Blast_Score=74, Evalue=6e-15, Organism=Escherichia coli, GI1789378, Length=249, Percent_Identity=27.3092369477912, Blast_Score=69, Evalue=4e-13, Organism=Escherichia coli, GI1787545, Length=257, Percent_Identity=26.0700389105058, Blast_Score=64, Evalue=1e-11, Organism=Caenorhabditis elegans, GI17560676, Length=248, Percent_Identity=34.6774193548387, Blast_Score=139, Evalue=9e-34, Organism=Caenorhabditis elegans, GI17561402, Length=252, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=1e-28, Organism=Caenorhabditis elegans, GI115534694, Length=250, Percent_Identity=32, Blast_Score=112, Evalue=1e-25, Organism=Caenorhabditis elegans, GI193204405, Length=264, Percent_Identity=34.0909090909091, Blast_Score=105, Evalue=2e-23, Organism=Caenorhabditis elegans, GI17560332, Length=263, Percent_Identity=31.9391634980989, Blast_Score=103, Evalue=1e-22, Organism=Caenorhabditis elegans, GI17551412, Length=262, Percent_Identity=30.1526717557252, Blast_Score=102, Evalue=2e-22, Organism=Caenorhabditis elegans, GI17560220, Length=262, Percent_Identity=32.4427480916031, Blast_Score=101, Evalue=4e-22, Organism=Caenorhabditis elegans, GI17531453, Length=261, Percent_Identity=32.183908045977, Blast_Score=100, Evalue=7e-22, Organism=Caenorhabditis elegans, GI17544670, Length=264, Percent_Identity=29.9242424242424, Blast_Score=100, Evalue=9e-22, Organism=Caenorhabditis elegans, GI71994604, Length=250, Percent_Identity=30, Blast_Score=100, Evalue=1e-21, Organism=Caenorhabditis elegans, GI17562910, Length=263, Percent_Identity=30.4182509505703, Blast_Score=99, Evalue=3e-21, Organism=Caenorhabditis elegans, GI17559104, Length=262, Percent_Identity=30.5343511450382, Blast_Score=99, Evalue=3e-21, Organism=Caenorhabditis elegans, GI71994600, Length=248, Percent_Identity=29.8387096774194, Blast_Score=98, Evalue=3e-21, Organism=Caenorhabditis elegans, GI17538480, Length=263, Percent_Identity=30.7984790874525, Blast_Score=97, Evalue=6e-21, Organism=Caenorhabditis elegans, GI17562906, Length=262, Percent_Identity=30.5343511450382, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI25147288, Length=247, Percent_Identity=29.5546558704453, Blast_Score=93, Evalue=1e-19, Organism=Caenorhabditis elegans, GI17508651, Length=249, Percent_Identity=28.5140562248996, Blast_Score=92, Evalue=4e-19, Organism=Caenorhabditis elegans, GI17562908, Length=263, Percent_Identity=30.4182509505703, Blast_Score=90, Evalue=1e-18, Organism=Caenorhabditis elegans, GI17560150, Length=262, Percent_Identity=28.2442748091603, Blast_Score=89, Evalue=3e-18, Organism=Caenorhabditis elegans, GI17555706, Length=251, Percent_Identity=29.4820717131474, Blast_Score=87, Evalue=6e-18, Organism=Caenorhabditis elegans, GI17538486, Length=269, Percent_Identity=29.7397769516729, Blast_Score=85, Evalue=3e-17, Organism=Caenorhabditis elegans, GI17565030, Length=262, Percent_Identity=29.3893129770992, Blast_Score=85, Evalue=3e-17, Organism=Caenorhabditis elegans, GI17562990, Length=263, Percent_Identity=28.1368821292776, Blast_Score=84, Evalue=6e-17, Organism=Caenorhabditis elegans, GI72000259, Length=261, Percent_Identity=29.1187739463602, Blast_Score=84, Evalue=6e-17, Organism=Caenorhabditis elegans, GI17562904, Length=267, Percent_Identity=26.9662921348315, Blast_Score=83, Evalue=2e-16, Organism=Caenorhabditis elegans, GI17561272, Length=254, Percent_Identity=28.3464566929134, Blast_Score=80, Evalue=1e-15, Organism=Caenorhabditis elegans, GI193207620, Length=223, Percent_Identity=30.4932735426009, Blast_Score=80, Evalue=1e-15, Organism=Caenorhabditis elegans, GI17558006, Length=255, Percent_Identity=28.6274509803922, Blast_Score=79, Evalue=3e-15, Organism=Caenorhabditis elegans, GI17532805, Length=226, Percent_Identity=29.646017699115, Blast_Score=78, Evalue=5e-15, Organism=Caenorhabditis elegans, GI17567345, Length=191, Percent_Identity=29.3193717277487, Blast_Score=77, Evalue=9e-15, Organism=Caenorhabditis elegans, GI17563726, Length=262, Percent_Identity=27.0992366412214, Blast_Score=75, Evalue=2e-14, Organism=Caenorhabditis elegans, GI17536025, Length=250, Percent_Identity=26, Blast_Score=75, Evalue=3e-14, Organism=Caenorhabditis elegans, GI32565532, Length=143, Percent_Identity=31.4685314685315, Blast_Score=74, Evalue=1e-13, Organism=Caenorhabditis elegans, GI71982365, Length=199, Percent_Identity=26.6331658291457, Blast_Score=70, Evalue=7e-13, Organism=Caenorhabditis elegans, GI17532791, Length=222, Percent_Identity=30.6306306306306, Blast_Score=69, Evalue=3e-12, Organism=Caenorhabditis elegans, GI17536651, Length=254, Percent_Identity=25.5905511811024, Blast_Score=69, Evalue=3e-12, Organism=Caenorhabditis elegans, GI193203117, Length=168, Percent_Identity=32.1428571428571, Blast_Score=67, Evalue=7e-12, Organism=Caenorhabditis elegans, GI71997099, Length=210, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=8e-12, Organism=Caenorhabditis elegans, GI193203115, Length=166, Percent_Identity=32.5301204819277, Blast_Score=67, Evalue=1e-11, Organism=Caenorhabditis elegans, GI17568967, Length=198, Percent_Identity=30.8080808080808, Blast_Score=66, Evalue=2e-11, Organism=Caenorhabditis elegans, GI71992402, Length=225, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=7e-11, Organism=Saccharomyces cerevisiae, GI6324126, Length=249, Percent_Identity=27.710843373494, Blast_Score=93, Evalue=4e-20, Organism=Saccharomyces cerevisiae, GI6322861, Length=194, Percent_Identity=35.0515463917526, Blast_Score=69, Evalue=5e-13, Organism=Drosophila melanogaster, GI21355319, Length=248, Percent_Identity=33.8709677419355, Blast_Score=142, Evalue=2e-34, Organism=Drosophila melanogaster, GI23397609, Length=248, Percent_Identity=32.6612903225806, Blast_Score=115, Evalue=2e-26, Organism=Drosophila melanogaster, GI24643142, Length=248, Percent_Identity=29.8387096774194, Blast_Score=104, Evalue=7e-23, Organism=Drosophila melanogaster, GI21357041, Length=252, Percent_Identity=31.3492063492063, Blast_Score=99, Evalue=2e-21, Organism=Drosophila melanogaster, GI24639444, Length=248, Percent_Identity=28.2258064516129, Blast_Score=97, Evalue=9e-21, Organism=Drosophila melanogaster, GI21358495, Length=203, Percent_Identity=31.5270935960591, Blast_Score=96, Evalue=2e-20, Organism=Drosophila melanogaster, GI28571526, Length=249, Percent_Identity=29.718875502008, Blast_Score=92, Evalue=3e-19, Organism=Drosophila melanogaster, GI24644339, Length=251, Percent_Identity=28.6852589641434, Blast_Score=89, Evalue=2e-18, Organism=Drosophila melanogaster, GI24644337, Length=251, Percent_Identity=30.6772908366534, Blast_Score=88, Evalue=5e-18, Organism=Drosophila melanogaster, GI24640492, Length=223, Percent_Identity=27.8026905829596, Blast_Score=88, Evalue=6e-18, Organism=Drosophila melanogaster, GI281364892, Length=247, Percent_Identity=29.1497975708502, Blast_Score=75, Evalue=5e-14, Organism=Drosophila melanogaster, GI24647946, Length=210, Percent_Identity=28.0952380952381, Blast_Score=69, Evalue=2e-12, Organism=Drosophila melanogaster, GI45553409, Length=210, Percent_Identity=28.0952380952381, Blast_Score=69, Evalue=3e-12, Organism=Drosophila melanogaster, GI24647948, Length=210, Percent_Identity=28.0952380952381, Blast_Score=69, Evalue=3e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: =1.1.1.14 [H]
Molecular weight: Translated: 26064; Mature: 26064
Theoretical pI: Translated: 9.13; Mature: 9.13
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 3.5 %Met (Translated Protein) 3.9 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 3.5 %Met (Mature Protein) 3.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MYKLFDMSGRVAVVTGSTKGMGLEMARALGASGARVVVSGRNAAISEKVAAALKSEGIDA CCEEEECCCCEEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCC TGLACDIADTASVRAFADRVLETFGRVDALVLNAAGSGVAGSILEQGPEDFDAVMAGNVR CCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHCCCCHHHHEEECCCC GNLVLVNALAPQMISRNDGSIIFMSSIAARRGSALLPLYSISKGAVDLAMRSLAHTLGPH CCEEEEEECCHHHHCCCCCCEEEEECHHHCCCCEEEEEHHCCCCHHHHHHHHHHHHCCCC NINVNSINPGPVRTDFSRDALWGNPDREKALSASIPMRRIAEARDVAGLAVLLASPAGRY CEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHEEEEEECCCCCE ISGQTIGLDGGATA ECCEEEECCCCCCC >Mature Secondary Structure MYKLFDMSGRVAVVTGSTKGMGLEMARALGASGARVVVSGRNAAISEKVAAALKSEGIDA CCEEEECCCCEEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCC TGLACDIADTASVRAFADRVLETFGRVDALVLNAAGSGVAGSILEQGPEDFDAVMAGNVR CCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHCCCCHHHHEEECCCC GNLVLVNALAPQMISRNDGSIIFMSSIAARRGSALLPLYSISKGAVDLAMRSLAHTLGPH CCEEEEEECCHHHHCCCCCCEEEEECHHHCCCCEEEEEHHCCCCHHHHHHHHHHHHCCCC NINVNSINPGPVRTDFSRDALWGNPDREKALSASIPMRRIAEARDVAGLAVLLASPAGRY CEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHEEEEEECCCCCE ISGQTIGLDGGATA ECCEEEECCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 7551049 [H]