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The map label for this gene is chaA [C]

Identifier: 94313796

GI number: 94313796

Start: 1536427

End: 1537605

Strand: Direct

Name: chaA [C]

Synonym: Rmet_4874

Alternate gene names: 94313796

Gene position: 1536427-1537605 (Clockwise)

Preceding gene: 291481544

Following gene: 94313801

Centisome position: 59.55

GC content: 65.39

Gene sequence:

>1179_bases
GTGGCCGCCCTTCGTTGCCAGAATATCTCTTGCGCGCGCGCCATCTGGCGTGCACGCTGTGCCTCTGCACGCCACAAAAA
GAAAGGAACACAAGGGATGGCAACTTCCAACAAGCTGCCGGCTTGGACCGTCTGGTCCCCGCTGGCTGCCTGGGCAGCGT
TCGGCCTGGGCCTTCTATTGACGGGACAGTCGTGGATGATCGCGGTGATGGCCGCTGCCCTGGCGGGCGCGGTATTTTCC
GCGGTGCACCATGCCGAGGTCGTCGCCCATAAGGTCGGCGAGCCGTTCGGCACCTTGGTGCTTGCGATTGCCGTGACGGT
AATCGAAGTGGCGCTGATTGTGTCGGTGATGCTGTCTTCCGGGCCTGAGAAGGCCGGTCTCGCCCGCGACACGGTGTTCG
CGGCGGTCATGATCATCTGCAACGGGATCGTCGGGGTCTGCCTGTTCGTCGGCGGCCTGCATCACCGCGAACAGGCGTTT
CAGGCGCCCGGCGCCAATGCCGCGATGGCCATCCTGGTTGCCCTGCTCGGCCTGACCATGGTGCTCCCCAACTTCACCAG
CTCGACGCCGGGACCCATGCTGTCCCCGTCGCAACTCGCCTTCGCGGGCGCCATGTCACTGGTGCTCTACGGCAGCTTCG
TGTTCGTGCAGACGGTGCGGCATCGCGACTACTTCCTGGTGGAAGGCAGCAGCGACGAGAACGAGCATGCGCCGCCGCCG
CCGGTGCGCGTCGCGGTAGTCAGTGGCGTGCTGCTGGTGGTCTGCCTGGTGGCCGTGGTGGCGCTGGCCAAGCTGCTGTC
GCCATCGGTGGAAGCGGCAGTACTGGGTGCCGGGCTGCCTCCTGCCGTGGTGGGTGTGGTGATTGCGGCGCTGGTGCTGC
TGCCTGAAGGGCTGGCCGCGTTGCGCGCGGCACGTTCGGACCGGATTCAGACCAGCCTCAACCTTGCGCTCGGCTCCGCG
CTGGCCAGTATCGGCCTGACGATTCCGACCGTGGCGGCCGTGTTCATCATGCTCAGCCGGCCGCTTGAACTCGGGCTTGG
CCCCAAGGAAATGGTCCTGCTGTTCCTGACCATGATCGTCGCCTCGCTGACGCTGGGCATGGGACGGACCACGATCCTTC
AGGGCGTGGTGCACATGGTGATTTTCGCCGCGTATCTGTTCCTGACGATCGTTCCGTAA

Upstream 100 bases:

>100_bases
GAAATCGGCGGGAAACAAGCCGGCAGCCACCGGCCCGGAAGTACTCGACACCGCCACCCGTCCACTGCCGCGCGACTGAT
ACTCGCCCCGGCCGGGAACT

Downstream 100 bases:

>100_bases
GGGTACGGTCTTTCGGTACTGTCTTTCGCCCCTCTCCCATAAATGGAGGCGGGGCGAAAAGCGACGGTTTACTTCTGCCG
CAGGAATCCGATCACGGCAT

Product: putative sodium/calcium exchanger membrane region

Products: Na (I) [Periplasm]; Ca (II) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MAALRCQNISCARAIWRARCASARHKKKGTQGMATSNKLPAWTVWSPLAAWAAFGLGLLLTGQSWMIAVMAAALAGAVFS
AVHHAEVVAHKVGEPFGTLVLAIAVTVIEVALIVSVMLSSGPEKAGLARDTVFAAVMIICNGIVGVCLFVGGLHHREQAF
QAPGANAAMAILVALLGLTMVLPNFTSSTPGPMLSPSQLAFAGAMSLVLYGSFVFVQTVRHRDYFLVEGSSDENEHAPPP
PVRVAVVSGVLLVVCLVAVVALAKLLSPSVEAAVLGAGLPPAVVGVVIAALVLLPEGLAALRAARSDRIQTSLNLALGSA
LASIGLTIPTVAAVFIMLSRPLELGLGPKEMVLLFLTMIVASLTLGMGRTTILQGVVHMVIFAAYLFLTIVP

Sequences:

>Translated_392_residues
MAALRCQNISCARAIWRARCASARHKKKGTQGMATSNKLPAWTVWSPLAAWAAFGLGLLLTGQSWMIAVMAAALAGAVFS
AVHHAEVVAHKVGEPFGTLVLAIAVTVIEVALIVSVMLSSGPEKAGLARDTVFAAVMIICNGIVGVCLFVGGLHHREQAF
QAPGANAAMAILVALLGLTMVLPNFTSSTPGPMLSPSQLAFAGAMSLVLYGSFVFVQTVRHRDYFLVEGSSDENEHAPPP
PVRVAVVSGVLLVVCLVAVVALAKLLSPSVEAAVLGAGLPPAVVGVVIAALVLLPEGLAALRAARSDRIQTSLNLALGSA
LASIGLTIPTVAAVFIMLSRPLELGLGPKEMVLLFLTMIVASLTLGMGRTTILQGVVHMVIFAAYLFLTIVP
>Mature_391_residues
AALRCQNISCARAIWRARCASARHKKKGTQGMATSNKLPAWTVWSPLAAWAAFGLGLLLTGQSWMIAVMAAALAGAVFSA
VHHAEVVAHKVGEPFGTLVLAIAVTVIEVALIVSVMLSSGPEKAGLARDTVFAAVMIICNGIVGVCLFVGGLHHREQAFQ
APGANAAMAILVALLGLTMVLPNFTSSTPGPMLSPSQLAFAGAMSLVLYGSFVFVQTVRHRDYFLVEGSSDENEHAPPPP
VRVAVVSGVLLVVCLVAVVALAKLLSPSVEAAVLGAGLPPAVVGVVIAALVLLPEGLAALRAARSDRIQTSLNLALGSAL
ASIGLTIPTVAAVFIMLSRPLELGLGPKEMVLLFLTMIVASLTLGMGRTTILQGVVHMVIFAAYLFLTIVP

Specific function: Possible cation transporter [H]

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Escherichia coli, GI1787468, Length=326, Percent_Identity=34.3558282208589, Blast_Score=187, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 40585; Mature: 40454

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AALRCQNISCARAIWRARCASARHKKKGTQGMATSNKLPAWTVWSPLAAWAAFGLGLLL
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TGQSWMIAVMAAALAGAVFSAVHHAEVVAHKVGEPFGTLVLAIAVTVIEVALIVSVMLSS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GPEKAGLARDTVFAAVMIICNGIVGVCLFVGGLHHREQAFQAPGANAAMAILVALLGLTM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VLPNFTSSTPGPMLSPSQLAFAGAMSLVLYGSFVFVQTVRHRDYFLVEGSSDENEHAPPP
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
PVRVAVVSGVLLVVCLVAVVALAKLLSPSVEAAVLGAGLPPAVVGVVIAALVLLPEGLAA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHH
LRAARSDRIQTSLNLALGSALASIGLTIPTVAAVFIMLSRPLELGLGPKEMVLLFLTMIV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHH
ASLTLGMGRTTILQGVVHMVIFAAYLFLTIVP
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm]; Ca (II) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + Na (I) [Periplasm] Ca (II) [Cytoplasm] + Proton [Periplasm] = Ca (II) [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]