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The map label for this gene is acrA [H]

Identifier: 94313789

GI number: 94313789

Start: 1528170

End: 1529405

Strand: Reverse

Name: acrA [H]

Synonym: Rmet_4867

Alternate gene names: 94313789

Gene position: 1529405-1528170 (Counterclockwise)

Preceding gene: 94313790

Following gene: 94313788

Centisome position: 59.28

GC content: 66.5

Gene sequence:

>1236_bases
ATGAATCAACAGTCTCGACGCACGCTGATTGCGATCGCCATCGTGGCGATCCTGGGCACCGGCGTGGCAACCTCGGTTCT
GCGTCCCTGGCACGGCGGGGAAGCACAGGCCAATACCCCGCCGCCGGCACCGGCGGTGGAAGTGTCCGCGGCGGTCGGCA
AGACCATCACCGAATGGGATGAATTCTCCGGCCGCATTGAAGCCGTGGACCGCGTGGAGATCCGTCCGCGTGTTTCCGGC
ACGATCGAAACCGTGCATTTCCGCGAGGGCTCGATCGTGAAGAAGGGCGATCCGCTGTTCACGATCGATCCGCGTCCGTA
TGCAGCCGAAGTGGCACGCGCCGAAGCGGCTTTGGCGGCGGCGCAGGTACGCGCATCGCTTGCGCAGACCGAGAAAGCCC
GCGCCCAGCGCCTGCTGGACGACAACGCGATCTCGCGCCGGGAGTTCGACGAGCGGATCAACGCCGCCAGCGAGATGTCG
GCCGACGTGCGCGGCGCGCAGGCGGCGCTGGAAGTGGCGAAGCTGAACCTGAGCTACACGCGTATCGTCGCGCCGGTCTC
GGGCAAGGTGTCGCGTGCCGAGATCACGGTGGGCAACCTGGTGGCCGCCGGCGCGGCGTCTCCGGCGCTGACCACGGTGG
TGTCCGTGTCGCCGGTGTATGCGAGCTTCGACGTCGACGAACAGAGCTACCTGCGCTACACGGCGCCGGGCGCCAGCGGC
GGCAAGAATGATCTGCCGGTGTTCCTGGGGCTGGCCAACGAGGACAACACGCCGCACGAAGGCAAGATCCACTCGGTGGA
CAACCGGCTGGATACGCGCAGCGGCACGATCCGCGTGCGAGCGATCTTCGCCAATGAAGATGGCCGCCTGCTGCCGGGCC
TGTACGCGAAGATCAAGCTCGGTGGCGGCACGCCGCATCCGGCCGTGCTGATCAATGATCGCGCCATCGGCACGGACCAG
GGCAAGAAGTTCGTGCTCGTGGTGGATCAGGCCAACAAGCTCACGTATCGCGAAGTCGAACTCGGGCCGACGTATGAAGG
GCTGCGTGTGATCCGCAAGGGCTTGAAGCCGGGCGAGAGCATCGTCGTCAATGGCCTGATGCGCGTGCGGCCAGGCGACA
CCGTTGCGCCGAAGCCGGTATCGATGGCCTTCCGCAGCGAGCTCGAGCCGCGAGGCAACGCGCCGGACCGCAAGCAGGCA
GCGGAAGAGAAGGCCGAGAAGCCCAACGTGAGCTAA

Upstream 100 bases:

>100_bases
ATTCCCGATTTCCGGGCCACTCGGCCCGGCGGGCGGCGTTCGGCCTCGATTTTTTGCTTTTCCAGTTGAAGAAACCCGAC
GAATCACGGAGTGAAGAAAA

Downstream 100 bases:

>100_bases
GCAACCCAGTCTAGCCAGACACAGACAATTTCCCCCCTTCGTGGCATCGAATGGCGTCCGCTTGCGAGCGGACCGCCAGG
GGGGCTGTTTTCGCCGAGAC

Product: multidrug transport protein, cation/multidrug efflux system, RND family efflux transporter MFP (membrane-fusion component)subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MNQQSRRTLIAIAIVAILGTGVATSVLRPWHGGEAQANTPPPAPAVEVSAAVGKTITEWDEFSGRIEAVDRVEIRPRVSG
TIETVHFREGSIVKKGDPLFTIDPRPYAAEVARAEAALAAAQVRASLAQTEKARAQRLLDDNAISRREFDERINAASEMS
ADVRGAQAALEVAKLNLSYTRIVAPVSGKVSRAEITVGNLVAAGAASPALTTVVSVSPVYASFDVDEQSYLRYTAPGASG
GKNDLPVFLGLANEDNTPHEGKIHSVDNRLDTRSGTIRVRAIFANEDGRLLPGLYAKIKLGGGTPHPAVLINDRAIGTDQ
GKKFVLVVDQANKLTYREVELGPTYEGLRVIRKGLKPGESIVVNGLMRVRPGDTVAPKPVSMAFRSELEPRGNAPDRKQA
AEEKAEKPNVS

Sequences:

>Translated_411_residues
MNQQSRRTLIAIAIVAILGTGVATSVLRPWHGGEAQANTPPPAPAVEVSAAVGKTITEWDEFSGRIEAVDRVEIRPRVSG
TIETVHFREGSIVKKGDPLFTIDPRPYAAEVARAEAALAAAQVRASLAQTEKARAQRLLDDNAISRREFDERINAASEMS
ADVRGAQAALEVAKLNLSYTRIVAPVSGKVSRAEITVGNLVAAGAASPALTTVVSVSPVYASFDVDEQSYLRYTAPGASG
GKNDLPVFLGLANEDNTPHEGKIHSVDNRLDTRSGTIRVRAIFANEDGRLLPGLYAKIKLGGGTPHPAVLINDRAIGTDQ
GKKFVLVVDQANKLTYREVELGPTYEGLRVIRKGLKPGESIVVNGLMRVRPGDTVAPKPVSMAFRSELEPRGNAPDRKQA
AEEKAEKPNVS
>Mature_411_residues
MNQQSRRTLIAIAIVAILGTGVATSVLRPWHGGEAQANTPPPAPAVEVSAAVGKTITEWDEFSGRIEAVDRVEIRPRVSG
TIETVHFREGSIVKKGDPLFTIDPRPYAAEVARAEAALAAAQVRASLAQTEKARAQRLLDDNAISRREFDERINAASEMS
ADVRGAQAALEVAKLNLSYTRIVAPVSGKVSRAEITVGNLVAAGAASPALTTVVSVSPVYASFDVDEQSYLRYTAPGASG
GKNDLPVFLGLANEDNTPHEGKIHSVDNRLDTRSGTIRVRAIFANEDGRLLPGLYAKIKLGGGTPHPAVLINDRAIGTDQ
GKKFVLVVDQANKLTYREVELGPTYEGLRVIRKGLKPGESIVVNGLMRVRPGDTVAPKPVSMAFRSELEPRGNAPDRKQA
AEEKAEKPNVS

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=340, Percent_Identity=33.2352941176471, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1786668, Length=343, Percent_Identity=33.8192419825073, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87082036, Length=346, Percent_Identity=30.3468208092486, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1789665, Length=320, Percent_Identity=31.5625, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87081796, Length=321, Percent_Identity=26.4797507788162, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081951, Length=203, Percent_Identity=33.4975369458128, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1789637, Length=141, Percent_Identity=36.8794326241135, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43927; Mature: 43927

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQQSRRTLIAIAIVAILGTGVATSVLRPWHGGEAQANTPPPAPAVEVSAAVGKTITEWD
CCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHCCHHHHHH
EFSGRIEAVDRVEIRPRVSGTIETVHFREGSIVKKGDPLFTIDPRPYAAEVARAEAALAA
HHCCCEEEEEEEEECCCCCCEEEEEEECCCCEEECCCCEEEECCCCHHHHHHHHHHHHHH
AQVRASLAQTEKARAQRLLDDNAISRREFDERINAASEMSADVRGAQAALEVAKLNLSYT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
RIVAPVSGKVSRAEITVGNLVAAGAASPALTTVVSVSPVYASFDVDEQSYLRYTAPGASG
EEEECCCCCCEEEEEEECCEEECCCCCCHHEEEEEECCEEEEECCCCCCEEEEECCCCCC
GKNDLPVFLGLANEDNTPHEGKIHSVDNRLDTRSGTIRVRAIFANEDGRLLPGLYAKIKL
CCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEEEE
GGGTPHPAVLINDRAIGTDQGKKFVLVVDQANKLTYREVELGPTYEGLRVIRKGLKPGES
CCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCCCC
IVVNGLMRVRPGDTVAPKPVSMAFRSELEPRGNAPDRKQAAEEKAEKPNVS
EEEECEEEECCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNQQSRRTLIAIAIVAILGTGVATSVLRPWHGGEAQANTPPPAPAVEVSAAVGKTITEWD
CCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHCCHHHHHH
EFSGRIEAVDRVEIRPRVSGTIETVHFREGSIVKKGDPLFTIDPRPYAAEVARAEAALAA
HHCCCEEEEEEEEECCCCCCEEEEEEECCCCEEECCCCEEEECCCCHHHHHHHHHHHHHH
AQVRASLAQTEKARAQRLLDDNAISRREFDERINAASEMSADVRGAQAALEVAKLNLSYT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
RIVAPVSGKVSRAEITVGNLVAAGAASPALTTVVSVSPVYASFDVDEQSYLRYTAPGASG
EEEECCCCCCEEEEEEECCEEECCCCCCHHEEEEEECCEEEEECCCCCCEEEEECCCCCC
GKNDLPVFLGLANEDNTPHEGKIHSVDNRLDTRSGTIRVRAIFANEDGRLLPGLYAKIKL
CCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEEEE
GGGTPHPAVLINDRAIGTDQGKKFVLVVDQANKLTYREVELGPTYEGLRVIRKGLKPGES
CCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCCCC
IVVNGLMRVRPGDTVAPKPVSMAFRSELEPRGNAPDRKQAAEEKAEKPNVS
EEEECEEEECCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]