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The map label for this gene is nasB [H]

Identifier: 94313742

GI number: 94313742

Start: 1468759

End: 1470000

Strand: Direct

Name: nasB [H]

Synonym: Rmet_4820

Alternate gene names: 94313742

Gene position: 1468759-1470000 (Clockwise)

Preceding gene: 94313741

Following gene: 94313743

Centisome position: 56.93

GC content: 67.47

Gene sequence:

>1242_bases
ATGACGCCCCCCGTCTCGTCCCACGCCAAGCCGCGACTGGTCGTCGTCGGCAATGGCATGGCCGGAATGCGCACGGTCGA
GGAACTGCTGAAGCTGGCGCCGGACCTGTATGACATCACAGTGTTCGGCGCCGAGCCGCACGGCAACTACAACCGCATTC
TGCTGTCCCCGGTGCTCGCCGGCGAAAAGACCGTCGACGACATCATGCTGAACACCCGAGCCTGGTATGGCCAGCATGGC
ATCGAGTTGCTGGCCGGCGATCCGGTCGTTGCAATCGACCGGCCGCGCCGGCTGGTGCGTTCCGCGTCCGGCAAGGAAGT
CCGCTATGACCGCCTGCTGCTGGCCACCGGCTCCAAGCCGTTCATCATCCCCGTCCCCGGCCACACGCTCGACGGCGTGA
TCGCGTTCCGCGATATCCAGGACGTGGAAACAATGCTCCACGCCGCTCGCAATCATCGCCATGCGGTGGTGATCGGCGGC
GGCCTGCTTGGCCTCGAAGCGGCCAACGGGCTGATGCGTCAGGGCATGGACGTCACCGTGGTGCACCTGGCCGATAGCCT
GATGGAGCGCCAGCTCGACAAACCTGCCGCCGAACTGCTCAAGGCAGCGCTGGAACGCAAGGGTCTGCGGTTCCTGCTCA
GCGCGCAGACCGCCGCCGTGCTCGGCAGCGAGCGGGTCACGGGCGTGCTGTTCAAAGACGGCACCCAGATCCCGGCGGAC
CTTGTGGTGATGACCGCCGGGGTGCGCCCGAACATCGAACTGGCCGCCAGCGCGGGGCTGCGCTGCGAGCGCGCGATCGT
CGTTGACGACACGCTGCAGACCTTCGACCCGCGCATCTACGCCGTGGGCGAATGCGTGCAGCACCGCCAGGCCACGTTCG
GGCTGGTGGCGCCGATCTGGGATCAGGCACGGGTGTGCGCCGCCCACCTGGCAGGTGCCGGCCACCGCCGCTATGTCCAG
CAAGCCACGGCTACCAAGCTCAAGGTCACGGGCGTGGACCTGTATTCGGCCGGCGACTTCATCGGCGGCGACAACAGCGA
AGACCTCGTGCTGCGCGATCCGCGCCGAGGCGTCTACAAACGTCTGGTGCTGCAAGGTGGCCGGCTCGTCGGTGCCGTGC
TCTATGGCGACGTCCAGGACGGCCCGTGGTACTTCGACCTGATCCAGACCGGCCAGCCGGTGGCCGCATTGCGTCAGCGC
CTGCTGTTCGGCAAGGCGCAATGCGAAGCCCTTGCGGCGTAG

Upstream 100 bases:

>100_bases
GCGAGGTGGAGGCAGAGGCGGAGGCCGAGGAAGAACTGGCCGCCTGACCTTCGATCGACACCCCTCGCCCACGCCAACAG
CCAGAATCAGGAAGACGCTC

Downstream 100 bases:

>100_bases
TTTTTCCGGGACATCGACGTGAACCTGTCAGATATCGCGGTGGTCGCCACCACCCCCACCAAGACAATCTCCACGACCTG
CCCCTACTGCGGCGTAGGCT

Product: assimilatory nitrate reductase (NADH) small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTPPVSSHAKPRLVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLAGEKTVDDIMLNTRAWYGQHG
IELLAGDPVVAIDRPRRLVRSASGKEVRYDRLLLATGSKPFIIPVPGHTLDGVIAFRDIQDVETMLHAARNHRHAVVIGG
GLLGLEAANGLMRQGMDVTVVHLADSLMERQLDKPAAELLKAALERKGLRFLLSAQTAAVLGSERVTGVLFKDGTQIPAD
LVVMTAGVRPNIELAASAGLRCERAIVVDDTLQTFDPRIYAVGECVQHRQATFGLVAPIWDQARVCAAHLAGAGHRRYVQ
QATATKLKVTGVDLYSAGDFIGGDNSEDLVLRDPRRGVYKRLVLQGGRLVGAVLYGDVQDGPWYFDLIQTGQPVAALRQR
LLFGKAQCEALAA

Sequences:

>Translated_413_residues
MTPPVSSHAKPRLVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLAGEKTVDDIMLNTRAWYGQHG
IELLAGDPVVAIDRPRRLVRSASGKEVRYDRLLLATGSKPFIIPVPGHTLDGVIAFRDIQDVETMLHAARNHRHAVVIGG
GLLGLEAANGLMRQGMDVTVVHLADSLMERQLDKPAAELLKAALERKGLRFLLSAQTAAVLGSERVTGVLFKDGTQIPAD
LVVMTAGVRPNIELAASAGLRCERAIVVDDTLQTFDPRIYAVGECVQHRQATFGLVAPIWDQARVCAAHLAGAGHRRYVQ
QATATKLKVTGVDLYSAGDFIGGDNSEDLVLRDPRRGVYKRLVLQGGRLVGAVLYGDVQDGPWYFDLIQTGQPVAALRQR
LLFGKAQCEALAA
>Mature_412_residues
TPPVSSHAKPRLVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLAGEKTVDDIMLNTRAWYGQHGI
ELLAGDPVVAIDRPRRLVRSASGKEVRYDRLLLATGSKPFIIPVPGHTLDGVIAFRDIQDVETMLHAARNHRHAVVIGGG
LLGLEAANGLMRQGMDVTVVHLADSLMERQLDKPAAELLKAALERKGLRFLLSAQTAAVLGSERVTGVLFKDGTQIPADL
VVMTAGVRPNIELAASAGLRCERAIVVDDTLQTFDPRIYAVGECVQHRQATFGLVAPIWDQARVCAAHLAGAGHRRYVQQ
ATATKLKVTGVDLYSAGDFIGGDNSEDLVLRDPRRGVYKRLVLQGGRLVGAVLYGDVQDGPWYFDLIQTGQPVAALRQRL
LFGKAQCEALAA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=290, Percent_Identity=26.8965517241379, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI65787454, Length=290, Percent_Identity=26.8965517241379, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI226437568, Length=290, Percent_Identity=26.8965517241379, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI22202629, Length=192, Percent_Identity=27.0833333333333, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4757732, Length=192, Percent_Identity=27.0833333333333, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789765, Length=389, Percent_Identity=36.7609254498715, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1789065, Length=328, Percent_Identity=28.6585365853659, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788892, Length=247, Percent_Identity=26.7206477732794, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=408, Percent_Identity=23.7745098039216, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281359715, Length=311, Percent_Identity=24.7588424437299, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI281359713, Length=311, Percent_Identity=24.7588424437299, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24639250, Length=311, Percent_Identity=24.7588424437299, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI18543267, Length=311, Percent_Identity=24.7588424437299, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24639252, Length=311, Percent_Identity=24.7588424437299, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24639257, Length=311, Percent_Identity=24.7588424437299, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24585130, Length=276, Percent_Identity=24.2753623188406, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21358499, Length=208, Percent_Identity=29.8076923076923, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581020, Length=196, Percent_Identity=26.0204081632653, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28573993, Length=196, Percent_Identity=26.0204081632653, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 44646; Mature: 44515

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPPVSSHAKPRLVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLA
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEHHC
GEKTVDDIMLNTRAWYGQHGIELLAGDPVVAIDRPRRLVRSASGKEVRYDRLLLATGSKP
CCCHHHHHHHCCCCCCCCCCCEEEECCCEEEECCCHHHHHCCCCCCEEEEEEEEEECCCC
FIIPVPGHTLDGVIAFRDIQDVETMLHAARNHRHAVVIGGGLLGLEAANGLMRQGMDVTV
EEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEECCEEEHHHHHHHHHCCCCEEE
VHLADSLMERQLDKPAAELLKAALERKGLRFLLSAQTAAVLGSERVTGVLFKDGTQIPAD
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCEEEEEEECCCCCCCE
LVVMTAGVRPNIELAASAGLRCERAIVVDDTLQTFDPRIYAVGECVQHRQATFGLVAPIW
EEEEECCCCCCEEEEECCCCCEEEEEEEECCHHHCCCCEEEHHHHHHHHHHHHEEECCHH
DQARVCAAHLAGAGHRRYVQQATATKLKVTGVDLYSAGDFIGGDNSEDLVLRDPRRGVYK
HHHHHHHHHHCCCCHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCCEEEECCHHHHHH
RLVLQGGRLVGAVLYGDVQDGPWYFDLIQTGQPVAALRQRLLFGKAQCEALAA
HHHHCCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCHHHHHHHCC
>Mature Secondary Structure 
TPPVSSHAKPRLVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLA
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEHHC
GEKTVDDIMLNTRAWYGQHGIELLAGDPVVAIDRPRRLVRSASGKEVRYDRLLLATGSKP
CCCHHHHHHHCCCCCCCCCCCEEEECCCEEEECCCHHHHHCCCCCCEEEEEEEEEECCCC
FIIPVPGHTLDGVIAFRDIQDVETMLHAARNHRHAVVIGGGLLGLEAANGLMRQGMDVTV
EEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEECCEEEHHHHHHHHHCCCCEEE
VHLADSLMERQLDKPAAELLKAALERKGLRFLLSAQTAAVLGSERVTGVLFKDGTQIPAD
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCEEEEEEECCCCCCCE
LVVMTAGVRPNIELAASAGLRCERAIVVDDTLQTFDPRIYAVGECVQHRQATFGLVAPIW
EEEEECCCCCCEEEEECCCCCEEEEEEEECCHHHCCCCEEEHHHHHHHHHHHHEEECCHH
DQARVCAAHLAGAGHRRYVQQATATKLKVTGVDLYSAGDFIGGDNSEDLVLRDPRRGVYK
HHHHHHHHHHCCCCHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCCEEEECCHHHHHH
RLVLQGGRLVGAVLYGDVQDGPWYFDLIQTGQPVAALRQRLLFGKAQCEALAA
HHHHCCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]