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The map label for this gene is nirD [H]

Identifier: 94313741

GI number: 94313741

Start: 1468304

End: 1468705

Strand: Direct

Name: nirD [H]

Synonym: Rmet_4819

Alternate gene names: 94313741

Gene position: 1468304-1468705 (Clockwise)

Preceding gene: 94313740

Following gene: 94313742

Centisome position: 56.91

GC content: 66.42

Gene sequence:

>402_bases
ATGAGCCAAGCCCACCACCCAGAGACCTGGACCGCGATTTGCACCTTGCGCGATATCGTGCCCAATACTGGTGTCTGCGC
CCTGGTCGACAGAAAGCAGGTCGCCGTCTTCCGCATCGGCCGCGGCGAGGAGGTCTACGCGCTGGCCAACTTCGACCCGA
ACGCGCAGGCAAACGTGCTGTCGCGTGGTCTGGTCGGCAACCTTGGCCCGCGCACCGTGGTGGCGTCGCCGATCTACAAG
CACCACTTCGATCTGCGCACCGGCGAATGCCTCGAAGCGCCCGAGCACTCGGTGGATGCTTATACCGCGCGGGTCTACGA
CGGCAAGGTCTGGGTGGCCACCGCAGCCACGGCCCGCGAGGTGGAGGCAGAGGCGGAGGCCGAGGAAGAACTGGCCGCCT
GA

Upstream 100 bases:

>100_bases
CAGGAGGAACGAAACTTGCAACGTTCCCGCCTGAGCCACATCCCCGTGGTTGCCGTGCCCGCAAAAGCAGCGTGAAGCCC
CCGATTTCAAGGAGAACGTG

Downstream 100 bases:

>100_bases
CCTTCGATCGACACCCCTCGCCCACGCCAACAGCCAGAATCAGGAAGACGCTCATGACGCCCCCCGTCTCGTCCCACGCC
AAGCCGCGACTGGTCGTCGT

Product: nitrite reductase, NAD(P)H-binding, small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 132

Protein sequence:

>133_residues
MSQAHHPETWTAICTLRDIVPNTGVCALVDRKQVAVFRIGRGEEVYALANFDPNAQANVLSRGLVGNLGPRTVVASPIYK
HHFDLRTGECLEAPEHSVDAYTARVYDGKVWVATAATAREVEAEAEAEEELAA

Sequences:

>Translated_133_residues
MSQAHHPETWTAICTLRDIVPNTGVCALVDRKQVAVFRIGRGEEVYALANFDPNAQANVLSRGLVGNLGPRTVVASPIYK
HHFDLRTGECLEAPEHSVDAYTARVYDGKVWVATAATAREVEAEAEAEEELAA
>Mature_132_residues
SQAHHPETWTAICTLRDIVPNTGVCALVDRKQVAVFRIGRGEEVYALANFDPNAQANVLSRGLVGNLGPRTVVASPIYKH
HFDLRTGECLEAPEHSVDAYTARVYDGKVWVATAATAREVEAEAEAEEELAA

Specific function: Required for activity of the reductase [H]

COG id: COG2146

COG function: function code PR; Ferredoxin subunits of nitrite reductase and ring-hydroxylating dioxygenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis nasE [H]

Homologues:

Organism=Escherichia coli, GI1789766, Length=101, Percent_Identity=46.5346534653465, Blast_Score=97, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR017941 [H]

Pfam domain/function: NA

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 14473; Mature: 14342

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAHHPETWTAICTLRDIVPNTGVCALVDRKQVAVFRIGRGEEVYALANFDPNAQANVL
CCCCCCCCCHHHEEEHHHHCCCCCEEEEECCCEEEEEEECCCCEEEEEECCCCCCCHHHH
SRGLVGNLGPRTVVASPIYKHHFDLRTGECLEAPEHSVDAYTARVYDGKVWVATAATARE
HCCCCCCCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEEECCCHHH
VEAEAEAEEELAA
HHHHHCHHHHHCC
>Mature Secondary Structure 
SQAHHPETWTAICTLRDIVPNTGVCALVDRKQVAVFRIGRGEEVYALANFDPNAQANVL
CCCCCCCCHHHEEEHHHHCCCCCEEEEECCCEEEEEEECCCCEEEEEECCCCCCCHHHH
SRGLVGNLGPRTVVASPIYKHHFDLRTGECLEAPEHSVDAYTARVYDGKVWVATAATARE
HCCCCCCCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEEECCCHHH
VEAEAEAEEELAA
HHHHHCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]