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The map label for this gene is 94313709

Identifier: 94313709

GI number: 94313709

Start: 1430778

End: 1432295

Strand: Direct

Name: 94313709

Synonym: Rmet_4787

Alternate gene names: NA

Gene position: 1430778-1432295 (Clockwise)

Preceding gene: 94313707

Following gene: 94313717

Centisome position: 55.45

GC content: 63.7

Gene sequence:

>1518_bases
ATGATGCCGTCTTCCGCCACATCCAATTTGCGCCCTCCTCCGATGTCCGCCTACCGTCCCGACCCCATCGTCATGCTGTC
TGCCCGACAGCTTTCCGAGGCCATTCACAAGAAGGAGGTGTCGAGCCGGGAAGTCATGACGGCCTATCTGGCGCACATCG
CCCGAGTCAACCCGGGCAGCAACGCCATCGTCGCCATGCAGGACGAGGAAGCGCTGATGCGCGAGGCATCGGCGGCCGAC
GAAGCGCTGGCGGCGGGCAAGTCTTCCGGCTGGATGCATGGCTTCCCGCAGGCGCCGAAGGATCTGGCGATGACGCGCGG
CATTCGCACAACGTACGGATCGCCGATCTTTCGCGACAATATCCCCGCCAGTGATTCGGTCATCGTAGAGCGTATGCGAC
GTGCCGGCTCGATCCTGATCGGCAAGACCAACACGCCTGAGTTCGGGCTGGGGTCGCACACGTTCAATCCCGTCTATGGC
GCCACGCTGAATCCCTACGATCCCACGCGCTCGGCTGGCGGCTCCAGTGGTGGTGCAGCCGCCGCGCTGGCGCTGCGTAT
GCTGCCGGTTGCCGATGGCAGCGACTTTGGCGGGTCGCTGCGCAATCCCGCTGCGTTCTGCAATGTCTATGGGTTCCGGC
CTTCAGCGGGACGCGTGCCTTACGGCCCGACGCCGGAGTTGTTTCTGCCCCAGTTGGGCAGCGAAGGGCCGATGGCACGC
AATGTCGAGGATCTCGCGCTACTGCTGGCCACGCAATCCGGCCCCGACGCGCGCACGCCGCAGGCGCTGCCACACGATCC
GATGCTGGCTTCGCTGACACCGGCAAACGTCGCGGACATACTGAAAACGGATCTCACCGGCAAGCGTGTTGCATGGCTGG
GCGACTGGGGTGGTTACCTGCCGATGGAAGACGGCATCCTGTCGCTCTGCGAGCAGGCGCTGCGCTTCTTCCCGGATTTC
GGCGTGGACGTCGAGGCCATCAAAGCACCCTACCCGCCCGAGAAAATCTGGCAAACCTGGCTTGTCTATCGCCATTTCCT
GACTGGGAATTCGCGTCTGGGGCTGTACACCAATCCGTCCACGCGTGCGCAGCTCAAGTCCGAGGCGATATGGGAAATCG
AAGGCTCGCTGGACCTCAAGGCCAGCGATCTGTACGCCGCCAGCACCGATCGCAGTGCGTGGTACCAGGCGCTGCAACGG
ACGTTCACCCAGTTCGACTACATCGCCGTGCCCACCGCACAGGTGTTTCCGTTCGACGTCACGCAACACTGGCCGAAGCA
GATCGCCGACAGGCAGATGGACACGTACCATCGCTGGATGGAAGTGGTGGTCGCGTGGACGCTATCGGGCTGCCCTGTGA
TCAGCGTGCCGGTGGGATTCAGCGCGGACGGATTGCCAATGGGGATGCAACTGATCGGCAGGCCGCGCGACGATCTGGGT
GTGCTGCAACTCGCAGCGGCCTATGAGAAGGTGCGCGACTGGGTCAATGATCGCCTGCCGCCGGCTATCGAAGGCTGA

Upstream 100 bases:

>100_bases
CTAGTGTCATTTTTGTGGTTTTGGGCAGGAATGATACGACATGGCGTGATCAACACGCCTCGCGGTCAACTCCCAATACA
CGAGCGCGGGCAATTCATTC

Downstream 100 bases:

>100_bases
GGGTTCTGCTCCCCTCTCACGTCGTGCAGGGAGGGGAGAAAACCGTACGGTACTTCAGGCCACCCGCCGCAACTGCCTCG
CTGCATTGGCAAACAACGGC

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MMPSSATSNLRPPPMSAYRPDPIVMLSARQLSEAIHKKEVSSREVMTAYLAHIARVNPGSNAIVAMQDEEALMREASAAD
EALAAGKSSGWMHGFPQAPKDLAMTRGIRTTYGSPIFRDNIPASDSVIVERMRRAGSILIGKTNTPEFGLGSHTFNPVYG
ATLNPYDPTRSAGGSSGGAAAALALRMLPVADGSDFGGSLRNPAAFCNVYGFRPSAGRVPYGPTPELFLPQLGSEGPMAR
NVEDLALLLATQSGPDARTPQALPHDPMLASLTPANVADILKTDLTGKRVAWLGDWGGYLPMEDGILSLCEQALRFFPDF
GVDVEAIKAPYPPEKIWQTWLVYRHFLTGNSRLGLYTNPSTRAQLKSEAIWEIEGSLDLKASDLYAASTDRSAWYQALQR
TFTQFDYIAVPTAQVFPFDVTQHWPKQIADRQMDTYHRWMEVVVAWTLSGCPVISVPVGFSADGLPMGMQLIGRPRDDLG
VLQLAAAYEKVRDWVNDRLPPAIEG

Sequences:

>Translated_505_residues
MMPSSATSNLRPPPMSAYRPDPIVMLSARQLSEAIHKKEVSSREVMTAYLAHIARVNPGSNAIVAMQDEEALMREASAAD
EALAAGKSSGWMHGFPQAPKDLAMTRGIRTTYGSPIFRDNIPASDSVIVERMRRAGSILIGKTNTPEFGLGSHTFNPVYG
ATLNPYDPTRSAGGSSGGAAAALALRMLPVADGSDFGGSLRNPAAFCNVYGFRPSAGRVPYGPTPELFLPQLGSEGPMAR
NVEDLALLLATQSGPDARTPQALPHDPMLASLTPANVADILKTDLTGKRVAWLGDWGGYLPMEDGILSLCEQALRFFPDF
GVDVEAIKAPYPPEKIWQTWLVYRHFLTGNSRLGLYTNPSTRAQLKSEAIWEIEGSLDLKASDLYAASTDRSAWYQALQR
TFTQFDYIAVPTAQVFPFDVTQHWPKQIADRQMDTYHRWMEVVVAWTLSGCPVISVPVGFSADGLPMGMQLIGRPRDDLG
VLQLAAAYEKVRDWVNDRLPPAIEG
>Mature_505_residues
MMPSSATSNLRPPPMSAYRPDPIVMLSARQLSEAIHKKEVSSREVMTAYLAHIARVNPGSNAIVAMQDEEALMREASAAD
EALAAGKSSGWMHGFPQAPKDLAMTRGIRTTYGSPIFRDNIPASDSVIVERMRRAGSILIGKTNTPEFGLGSHTFNPVYG
ATLNPYDPTRSAGGSSGGAAAALALRMLPVADGSDFGGSLRNPAAFCNVYGFRPSAGRVPYGPTPELFLPQLGSEGPMAR
NVEDLALLLATQSGPDARTPQALPHDPMLASLTPANVADILKTDLTGKRVAWLGDWGGYLPMEDGILSLCEQALRFFPDF
GVDVEAIKAPYPPEKIWQTWLVYRHFLTGNSRLGLYTNPSTRAQLKSEAIWEIEGSLDLKASDLYAASTDRSAWYQALQR
TFTQFDYIAVPTAQVFPFDVTQHWPKQIADRQMDTYHRWMEVVVAWTLSGCPVISVPVGFSADGLPMGMQLIGRPRDDLG
VLQLAAAYEKVRDWVNDRLPPAIEG

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=252, Percent_Identity=37.3015873015873, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI222831590, Length=511, Percent_Identity=25.6360078277886, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI166795287, Length=215, Percent_Identity=28.8372093023256, Blast_Score=85, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=516, Percent_Identity=25.968992248062, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17556264, Length=306, Percent_Identity=29.7385620915033, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=240, Percent_Identity=30.4166666666667, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=226, Percent_Identity=31.4159292035398, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=187, Percent_Identity=35.2941176470588, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=199, Percent_Identity=30.1507537688442, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=199, Percent_Identity=30.1507537688442, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=235, Percent_Identity=28.5106382978723, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=442, Percent_Identity=26.9230769230769, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=228, Percent_Identity=28.9473684210526, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=176, Percent_Identity=32.9545454545455, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550774, Length=271, Percent_Identity=34.6863468634686, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24648435, Length=271, Percent_Identity=34.6863468634686, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648437, Length=271, Percent_Identity=34.6863468634686, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078093, Length=511, Percent_Identity=24.26614481409, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24644968, Length=260, Percent_Identity=33.0769230769231, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24652985, Length=256, Percent_Identity=34.765625, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922090, Length=256, Percent_Identity=34.765625, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24652981, Length=256, Percent_Identity=34.765625, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24652983, Length=256, Percent_Identity=34.765625, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356731, Length=513, Percent_Identity=24.9512670565302, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648113, Length=237, Percent_Identity=34.1772151898734, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648441, Length=166, Percent_Identity=38.5542168674699, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648439, Length=166, Percent_Identity=38.5542168674699, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 54982; Mature: 54982

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPSSATSNLRPPPMSAYRPDPIVMLSARQLSEAIHKKEVSSREVMTAYLAHIARVNPGS
CCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NAIVAMQDEEALMREASAADEALAAGKSSGWMHGFPQAPKDLAMTRGIRTTYGSPIFRDN
CEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCHHHCCCCCCCCC
IPASDSVIVERMRRAGSILIGKTNTPEFGLGSHTFNPVYGATLNPYDPTRSAGGSSGGAA
CCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
AALALRMLPVADGSDFGGSLRNPAAFCNVYGFRPSAGRVPYGPTPELFLPQLGSEGPMAR
HHHHHHHEECCCCCCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCC
NVEDLALLLATQSGPDARTPQALPHDPMLASLTPANVADILKTDLTGKRVAWLGDWGGYL
CHHHHHEEEEECCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCCCEEEEEECCCCCC
PMEDGILSLCEQALRFFPDFGVDVEAIKAPYPPEKIWQTWLVYRHFLTGNSRLGLYTNPS
CCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC
TRAQLKSEAIWEIEGSLDLKASDLYAASTDRSAWYQALQRTFTQFDYIAVPTAQVFPFDV
HHHHHHHCCEEEECCCCEEECCCCEECCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCH
TQHWPKQIADRQMDTYHRWMEVVVAWTLSGCPVISVPVGFSADGLPMGMQLIGRPRDDLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHCCCCHHHH
VLQLAAAYEKVRDWVNDRLPPAIEG
HHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMPSSATSNLRPPPMSAYRPDPIVMLSARQLSEAIHKKEVSSREVMTAYLAHIARVNPGS
CCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NAIVAMQDEEALMREASAADEALAAGKSSGWMHGFPQAPKDLAMTRGIRTTYGSPIFRDN
CEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCHHHCCCCCCCCC
IPASDSVIVERMRRAGSILIGKTNTPEFGLGSHTFNPVYGATLNPYDPTRSAGGSSGGAA
CCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
AALALRMLPVADGSDFGGSLRNPAAFCNVYGFRPSAGRVPYGPTPELFLPQLGSEGPMAR
HHHHHHHEECCCCCCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCC
NVEDLALLLATQSGPDARTPQALPHDPMLASLTPANVADILKTDLTGKRVAWLGDWGGYL
CHHHHHEEEEECCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCCCEEEEEECCCCCC
PMEDGILSLCEQALRFFPDFGVDVEAIKAPYPPEKIWQTWLVYRHFLTGNSRLGLYTNPS
CCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC
TRAQLKSEAIWEIEGSLDLKASDLYAASTDRSAWYQALQRTFTQFDYIAVPTAQVFPFDV
HHHHHHHCCEEEECCCCEEECCCCEECCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCH
TQHWPKQIADRQMDTYHRWMEVVVAWTLSGCPVISVPVGFSADGLPMGMQLIGRPRDDLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHCCCCHHHH
VLQLAAAYEKVRDWVNDRLPPAIEG
HHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]