Click here to switch to the map view.

The map label for this gene is degS [H]

Identifier: 94313636

GI number: 94313636

Start: 1356730

End: 1358073

Strand: Direct

Name: degS [H]

Synonym: Rmet_4714

Alternate gene names: 94313636

Gene position: 1356730-1358073 (Clockwise)

Preceding gene: 94313615

Following gene: 94313640

Centisome position: 52.58

GC content: 62.43

Gene sequence:

>1344_bases
ATGGGTAGAGACCCTCCTTCTTCCTCCGACGGGCCGCTTTCATGGCATTGCATTTTTCACGCGCCTAGAATGATCACAAA
CGATACAAGGCACGCATCGGGAGTGCCGGCCCGTCGCAGTCGGGAACACAGGCCCGCGTGGTCCGGGCCACCATGGCGCG
CCTTTCATCGGAACGTGGGAATCGCGCGAATCTGTATCGGAGGGCCAATGGAAGACAAGCCATCCCATGGAGCCGAGCCC
CTGAAGTTGCGTGACGTCACGGATGCGATGCGCCGGATGAGCGCGGCCATCGAAACGGGTCACCGGTGCTTTCGTGCCAT
TACCGACGGACGAGGCACGGCGCTCGCAGTTCTGGCGCCTGACGGGTACTTCGTATCTGTCAACCATGGCGCCGCCAGAT
TGCTCGGCTACACCTGCGCGGAGCTGATCGGCCGGTCACTGGCCGACATGGCAGACGACGGCGATCGCGCATGGATCGTG
GATTGCCTGGCCGCGAGCATCTCCTGCACCCTCCGCTCGTTCAATGTCGTGCTGCATGGCCGTGACAATCGAGCAGCCAG
TGTCCTGCTGCATTTTCAGCCGGTGATGGATGGCAGGGGCAACTGCAGCGCGGTGCTTGTCATCTTCGAGGAGCCTGTCG
TGCTGCAGCAGGGGCTGGTGGATGCCCTGATGCGGTCGGAAGCGGATTTCCGGCGTCTGTACACGCACCTGCTGACCGGT
CAGGAACACGAGCGCAAGCGCCTGTCTTCAGAGCTTCACGACGGCCTTGGGCAGGCGCTGACGCTGGTCAAGCTGATGGT
GGAGGATGCGCTTGGGCGGATGCGCCATGGCAGCGCCGAGCAGGCGGAGTCACTGCTCGAAACCACGCTGCTACGCATCA
GGGAAACCATCGGCGAGGTCCGGCACATCTGCAATGACCTGCGCCCACGGCTGCTGGACGATCTGGGCCTGGTGCCGGCG
CTGGAGGCGCTGTGCAAGCAGACGCAGCAATGCACCGGCAATCTGCTGGTGGTCTTCGACTGTCGCATCGTTGAAAACGA
GGTGCCCGACAACCTCAAGGCGGACATTTTCCGGATTGCCCAAGAGGCGATCAACAACATCATCAAGCACGCGCTGGCCA
CGGAGATCAACATCGTCCTGCGCCGCGCCGCGAAGGAGATTCTGCTGACGATTCAGGACAATGGCATTGGCTTCGACGAT
CTATCCAGCTCACCCGACGGCCCGACATTCTCCGGCCTTGGTCTTCTGGGCATGCGCGAGCGGGTCGAATCGAACGGAGG
GAGCTTTGCGCTGACGTCAGGCCAAGGTAGCGGAACCCTGGTCAGCGCTGCATGGAGGGTCTGA

Upstream 100 bases:

>100_bases
TCCCTTCCTCCTGGTGGAGAGCTGCGATCCAACCTGGGGCCTTAGTGACATTCTTGGATACAGGGCGGGTGCCAAGTAGT
AGGGAGGTCCGCCTTTAGGC

Downstream 100 bases:

>100_bases
TCGGGCAGACCCTCCCGCTACCCTGTCACTCGTTCCGCAGTCATGGGGAAGCCTCACCATCCGAACCGGCGGCGCGCCCC
TCGTCCAGCATCTGCCGGCG

Product: Two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MGRDPPSSSDGPLSWHCIFHAPRMITNDTRHASGVPARRSREHRPAWSGPPWRAFHRNVGIARICIGGPMEDKPSHGAEP
LKLRDVTDAMRRMSAAIETGHRCFRAITDGRGTALAVLAPDGYFVSVNHGAARLLGYTCAELIGRSLADMADDGDRAWIV
DCLAASISCTLRSFNVVLHGRDNRAASVLLHFQPVMDGRGNCSAVLVIFEEPVVLQQGLVDALMRSEADFRRLYTHLLTG
QEHERKRLSSELHDGLGQALTLVKLMVEDALGRMRHGSAEQAESLLETTLLRIRETIGEVRHICNDLRPRLLDDLGLVPA
LEALCKQTQQCTGNLLVVFDCRIVENEVPDNLKADIFRIAQEAINNIIKHALATEINIVLRRAAKEILLTIQDNGIGFDD
LSSSPDGPTFSGLGLLGMRERVESNGGSFALTSGQGSGTLVSAAWRV

Sequences:

>Translated_447_residues
MGRDPPSSSDGPLSWHCIFHAPRMITNDTRHASGVPARRSREHRPAWSGPPWRAFHRNVGIARICIGGPMEDKPSHGAEP
LKLRDVTDAMRRMSAAIETGHRCFRAITDGRGTALAVLAPDGYFVSVNHGAARLLGYTCAELIGRSLADMADDGDRAWIV
DCLAASISCTLRSFNVVLHGRDNRAASVLLHFQPVMDGRGNCSAVLVIFEEPVVLQQGLVDALMRSEADFRRLYTHLLTG
QEHERKRLSSELHDGLGQALTLVKLMVEDALGRMRHGSAEQAESLLETTLLRIRETIGEVRHICNDLRPRLLDDLGLVPA
LEALCKQTQQCTGNLLVVFDCRIVENEVPDNLKADIFRIAQEAINNIIKHALATEINIVLRRAAKEILLTIQDNGIGFDD
LSSSPDGPTFSGLGLLGMRERVESNGGSFALTSGQGSGTLVSAAWRV
>Mature_446_residues
GRDPPSSSDGPLSWHCIFHAPRMITNDTRHASGVPARRSREHRPAWSGPPWRAFHRNVGIARICIGGPMEDKPSHGAEPL
KLRDVTDAMRRMSAAIETGHRCFRAITDGRGTALAVLAPDGYFVSVNHGAARLLGYTCAELIGRSLADMADDGDRAWIVD
CLAASISCTLRSFNVVLHGRDNRAASVLLHFQPVMDGRGNCSAVLVIFEEPVVLQQGLVDALMRSEADFRRLYTHLLTGQ
EHERKRLSSELHDGLGQALTLVKLMVEDALGRMRHGSAEQAESLLETTLLRIRETIGEVRHICNDLRPRLLDDLGLVPAL
EALCKQTQQCTGNLLVVFDCRIVENEVPDNLKADIFRIAQEAINNIIKHALATEINIVLRRAAKEILLTIQDNGIGFDDL
SSSPDGPTFSGLGLLGMRERVESNGGSFALTSGQGSGTLVSAAWRV

Specific function: Member of the two-component regulatory system degS/degU. Involved in a sensory transduction pathway that affect the production of enzymes that degrade polymeric carbon and nitrogen sources. DegS probably acts as a kinase that phosphorylates degU [H]

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=226, Percent_Identity=30.9734513274336, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787474, Length=231, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788812, Length=204, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR008595
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR016381 [H]

Pfam domain/function: PF05384 DegS; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48876; Mature: 48744

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50112 PAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRDPPSSSDGPLSWHCIFHAPRMITNDTRHASGVPARRSREHRPAWSGPPWRAFHRNVG
CCCCCCCCCCCCEEEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCC
IARICIGGPMEDKPSHGAEPLKLRDVTDAMRRMSAAIETGHRCFRAITDGRGTALAVLAP
EEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
DGYFVSVNHGAARLLGYTCAELIGRSLADMADDGDRAWIVDCLAASISCTLRSFNVVLHG
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEEEEEC
RDNRAASVLLHFQPVMDGRGNCSAVLVIFEEPVVLQQGLVDALMRSEADFRRLYTHLLTG
CCCCEEEEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHCC
QEHERKRLSSELHDGLGQALTLVKLMVEDALGRMRHGSAEQAESLLETTLLRIRETIGEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RHICNDLRPRLLDDLGLVPALEALCKQTQQCTGNLLVVFDCRIVENEVPDNLKADIFRIA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCHHHHHHHHH
QEAINNIIKHALATEINIVLRRAAKEILLTIQDNGIGFDDLSSSPDGPTFSGLGLLGMRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHH
RVESNGGSFALTSGQGSGTLVSAAWRV
HHHCCCCCEEEECCCCCCCEEECCCCC
>Mature Secondary Structure 
GRDPPSSSDGPLSWHCIFHAPRMITNDTRHASGVPARRSREHRPAWSGPPWRAFHRNVG
CCCCCCCCCCCEEEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCC
IARICIGGPMEDKPSHGAEPLKLRDVTDAMRRMSAAIETGHRCFRAITDGRGTALAVLAP
EEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
DGYFVSVNHGAARLLGYTCAELIGRSLADMADDGDRAWIVDCLAASISCTLRSFNVVLHG
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEEEEEC
RDNRAASVLLHFQPVMDGRGNCSAVLVIFEEPVVLQQGLVDALMRSEADFRRLYTHLLTG
CCCCEEEEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHCC
QEHERKRLSSELHDGLGQALTLVKLMVEDALGRMRHGSAEQAESLLETTLLRIRETIGEV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RHICNDLRPRLLDDLGLVPALEALCKQTQQCTGNLLVVFDCRIVENEVPDNLKADIFRIA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCHHHHHHHHH
QEAINNIIKHALATEINIVLRRAAKEILLTIQDNGIGFDDLSSSPDGPTFSGLGLLGMRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHH
RVESNGGSFALTSGQGSGTLVSAAWRV
HHHCCCCCEEEECCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3141378; 3141377; 9384377; 3136143 [H]