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The map label for this gene is yomI [H]

Identifier: 94313615

GI number: 94313615

Start: 1329072

End: 1329719

Strand: Direct

Name: yomI [H]

Synonym: Rmet_4693

Alternate gene names: 94313615

Gene position: 1329072-1329719 (Clockwise)

Preceding gene: 94313613

Following gene: 94313636

Centisome position: 51.51

GC content: 63.27

Gene sequence:

>648_bases
ATGTACTGGGAGGGGACGCCAATGACTGAGCGCAAGCCATCAACGTTGCCGAGGACCATCCTTTTCACGGTCGTGGTGAT
CGTGGGGCTTGCCTTTGCGCGCGTGGCACGGGCCCAGATCTTCGGCGCGGTCAGTGCGGACGGGTCCATCACGCTGACCA
ATACGGCGGGAAATCCACATCTGCACCTGCTGGTCGCCGGCAATCCACCGGCCGAGCGCAAGCCCCGGGAGCCGAGGGAT
CTGCCCACCGACGCCGCGCTGTTCGCGACTTTCATTTCGGAAGCCAGCCGCAACTCCCACCTGCCGCCGGAACTGATCCG
TGCCGTGATTGCCATCGAATCGCGCTTCAATCCCAATGCGGTATCCAGAAAGGGCGCGATCGGACTGATGCAACTGATGC
CGGATACCGCCAAGCGGTTCTCGACCGGCGACATGCTGAATCCGCGCGACAACATACTCGCGGGCGCACAGTACCTGCGC
TATCTGCTGGACCTGTTCCGCGATGATCTGGAACTTGCGCTGGCGGCCTACAACGCTGGCGAGAATGCGGTGATCCAGGC
GGGAAACCGGATCCCGGCCTACGCGGAAACGCAGGCCTACGTACCGATGGTCATGGCGCACTACCGCAGGCTGCTGGCCT
CGGGGTAA

Upstream 100 bases:

>100_bases
GCACGGGGCGCCAGGAACCCCACAATCCCTACAACTCCCACAATCCCTACTGCGTCATGGGGATACTTCCTCCTGCCGCA
ATGGGTCGGGACGTACCAGA

Downstream 100 bases:

>100_bases
TGCGCCGGCGATGGCCGGTGCTGCTGCTTTACTGCCCTACGGCTCTACGGCTCTACGGCTCTACGGCTCTACGGCTCTAC
GGCTCTACTGCCGGATCGTC

Product: putative lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MYWEGTPMTERKPSTLPRTILFTVVVIVGLAFARVARAQIFGAVSADGSITLTNTAGNPHLHLLVAGNPPAERKPREPRD
LPTDAALFATFISEASRNSHLPPELIRAVIAIESRFNPNAVSRKGAIGLMQLMPDTAKRFSTGDMLNPRDNILAGAQYLR
YLLDLFRDDLELALAAYNAGENAVIQAGNRIPAYAETQAYVPMVMAHYRRLLASG

Sequences:

>Translated_215_residues
MYWEGTPMTERKPSTLPRTILFTVVVIVGLAFARVARAQIFGAVSADGSITLTNTAGNPHLHLLVAGNPPAERKPREPRD
LPTDAALFATFISEASRNSHLPPELIRAVIAIESRFNPNAVSRKGAIGLMQLMPDTAKRFSTGDMLNPRDNILAGAQYLR
YLLDLFRDDLELALAAYNAGENAVIQAGNRIPAYAETQAYVPMVMAHYRRLLASG
>Mature_215_residues
MYWEGTPMTERKPSTLPRTILFTVVVIVGLAFARVARAQIFGAVSADGSITLTNTAGNPHLHLLVAGNPPAERKPREPRD
LPTDAALFATFISEASRNSHLPPELIRAVIAIESRFNPNAVSRKGAIGLMQLMPDTAKRFSTGDMLNPRDNILAGAQYLR
YLLDLFRDDLELALAAYNAGENAVIQAGNRIPAYAETQAYVPMVMAHYRRLLASG

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 10 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=143, Percent_Identity=35.6643356643357, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR008258
- InterPro:   IPR016047
- InterPro:   IPR010090
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01551 Peptidase_M23; PF10145 PhageMin_Tail; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 23485; Mature: 23485

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYWEGTPMTERKPSTLPRTILFTVVVIVGLAFARVARAQIFGAVSADGSITLTNTAGNPH
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCE
LHLLVAGNPPAERKPREPRDLPTDAALFATFISEASRNSHLPPELIRAVIAIESRFNPNA
EEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCH
VSRKGAIGLMQLMPDTAKRFSTGDMLNPRDNILAGAQYLRYLLDLFRDDLELALAAYNAG
HCCCCCHHHHHHCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENAVIQAGNRIPAYAETQAYVPMVMAHYRRLLASG
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYWEGTPMTERKPSTLPRTILFTVVVIVGLAFARVARAQIFGAVSADGSITLTNTAGNPH
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCE
LHLLVAGNPPAERKPREPRDLPTDAALFATFISEASRNSHLPPELIRAVIAIESRFNPNA
EEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCH
VSRKGAIGLMQLMPDTAKRFSTGDMLNPRDNILAGAQYLRYLLDLFRDDLELALAAYNAG
HCCCCCHHHHHHCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENAVIQAGNRIPAYAETQAYVPMVMAHYRRLLASG
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]