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The map label for this gene is nodT [H]

Identifier: 94313541

GI number: 94313541

Start: 1245794

End: 1247224

Strand: Reverse

Name: nodT [H]

Synonym: Rmet_4619

Alternate gene names: 94313541

Gene position: 1247224-1245794 (Counterclockwise)

Preceding gene: 94313542

Following gene: 94313540

Centisome position: 48.34

GC content: 66.88

Gene sequence:

>1431_bases
ATGTCGTCATCTCAGATCAAGAAGTCCCTGCTGCTGGCGCTGCCGGTTGCCATGCTTGCCGGGTGCGCCCTGCAGACCCC
GCCAAAGGGCGAGGACCTTCGCCAACAAACCGTTCCCTCGCTCGAAGGGCGGGCCGGGTGGGCGAACCCGACCTCGGGCG
ACGCTGTCGCCGATGGCTGGCTTGCCGCGTTCGACGATGCCGAACTGCAACGGCTCGTGCGGGAAGCCATCGCGCACAAC
GGCGATCTGCGACTTGCGGAGGCGCGTGTGCAGCAGGCGCAGGCGCTCGTTGCCATCGCACGGAGCGGGCTGCTGCCAAG
CGCTGGCGTGAAGGGCAGGGCGGGCAACTCGGAAACGCAGATCCTCTCCATCGGCGCAAGCTGGGAGGTGGACCTCTGGG
GGCGCATTCGCGCGGAGTCCCGCTCGGCCGAATCGCAGTACGCCGCCACACAGGACGACTATCTCTGGGCGCAGCGCGTG
ATTGCGGCGACCACTGCCAAGGCATGGTTCTCGCTGATCCGATACACCGTGCTCGAAGACCGGTTGCGGCAATCCGCCAC
CATTCAGGAGGAGCTGGTGCGAATCGTCGGCCAACGGGTGACCATCGGCATTGCGCCGGAGACCGACCTGCTGGAAGCCA
GGAACACCCTGCACGCACAGCGCGACGGCATCGAGAAGACCGCACTGGCCAGGAGCCAGGCCGCGCAGGCGCTGGAACTG
CTGCTGGGCCGCTACCCGGCCGGAGACATCGCGACGGCGCCGACCCTGCCTGCCATGCCGCCCGCGCCGAACGCGAGCCT
GCCGGCGAATGTGGTCGAGCGTCGCCCCGACCTGACTGCCGCCGCGCATCGCGTTGCGGCGGCGTTCGATATGCGGCAAT
CGGCGGAAGCGGCACGTCTGCCACGCATCTCGATCAGCGCAGCCGTTACCGGTATCCGCAGCGATGTGTTCCTGCTCAAT
CAGGCCGGTAGCCCGGTCAAGGGTCTGAACGGTGCCTTCTTTGCCCCGATCTTCATGGGTGGCGAGCTGAAGGCCCGTGT
GGACTACTACACGGCAGAGCAGAAGGCGGCACTGATCGCCTATGGCAACAGTGCGCTGCGGGCACTGGGCGAGGTCGAAA
CCGGGCTACGGGCAGAGTCTGGCTATGCCGAACGCACCAGGCAACTCCGCGAGCGCGTGAACGAGCGTCGGGTGCTGGTG
CAGCGAGAGGAAACACGCGTGACGATCGGCGCGTCGGACACACGGAGTCTGCAGCAGCAGCGGCAGACGCTGGTGGCGGC
CGAGATGGATCTCGTGAACGTGGAGGCCGATCACCTTGATCAGCGCGTGGCGTTGTTGCTGGCGCTGGGCGGGGACTGGA
GGCCGGAAACCACAGCCACAGCTACAGCTACAGCTACAGCTACAGGTACGGGGACGGGGACGGGGACATAA

Upstream 100 bases:

>100_bases
CATGGAGATGTTCCATATCATCCGCATGGTCTTCCTGCGCGTCAGTTCCAAGCTGAACTACCTCGTGTTCAAGCTGCACT
AGGCCACGGGAGACAAGCTT

Downstream 100 bases:

>100_bases
GAACCTGGGCACTGTGACTAGTCACAGTAGACGCCTTCACGGCGACCTTTCATCATGCGGGCAGGTTTCCAGACGACGAA
GTCACCCCCATGCACCCCCG

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSSSQIKKSLLLALPVAMLAGCALQTPPKGEDLRQQTVPSLEGRAGWANPTSGDAVADGWLAAFDDAELQRLVREAIAHN
GDLRLAEARVQQAQALVAIARSGLLPSAGVKGRAGNSETQILSIGASWEVDLWGRIRAESRSAESQYAATQDDYLWAQRV
IAATTAKAWFSLIRYTVLEDRLRQSATIQEELVRIVGQRVTIGIAPETDLLEARNTLHAQRDGIEKTALARSQAAQALEL
LLGRYPAGDIATAPTLPAMPPAPNASLPANVVERRPDLTAAAHRVAAAFDMRQSAEAARLPRISISAAVTGIRSDVFLLN
QAGSPVKGLNGAFFAPIFMGGELKARVDYYTAEQKAALIAYGNSALRALGEVETGLRAESGYAERTRQLRERVNERRVLV
QREETRVTIGASDTRSLQQQRQTLVAAEMDLVNVEADHLDQRVALLLALGGDWRPETTATATATATATGTGTGTGT

Sequences:

>Translated_476_residues
MSSSQIKKSLLLALPVAMLAGCALQTPPKGEDLRQQTVPSLEGRAGWANPTSGDAVADGWLAAFDDAELQRLVREAIAHN
GDLRLAEARVQQAQALVAIARSGLLPSAGVKGRAGNSETQILSIGASWEVDLWGRIRAESRSAESQYAATQDDYLWAQRV
IAATTAKAWFSLIRYTVLEDRLRQSATIQEELVRIVGQRVTIGIAPETDLLEARNTLHAQRDGIEKTALARSQAAQALEL
LLGRYPAGDIATAPTLPAMPPAPNASLPANVVERRPDLTAAAHRVAAAFDMRQSAEAARLPRISISAAVTGIRSDVFLLN
QAGSPVKGLNGAFFAPIFMGGELKARVDYYTAEQKAALIAYGNSALRALGEVETGLRAESGYAERTRQLRERVNERRVLV
QREETRVTIGASDTRSLQQQRQTLVAAEMDLVNVEADHLDQRVALLLALGGDWRPETTATATATATATGTGTGTGT
>Mature_475_residues
SSSQIKKSLLLALPVAMLAGCALQTPPKGEDLRQQTVPSLEGRAGWANPTSGDAVADGWLAAFDDAELQRLVREAIAHNG
DLRLAEARVQQAQALVAIARSGLLPSAGVKGRAGNSETQILSIGASWEVDLWGRIRAESRSAESQYAATQDDYLWAQRVI
AATTAKAWFSLIRYTVLEDRLRQSATIQEELVRIVGQRVTIGIAPETDLLEARNTLHAQRDGIEKTALARSQAAQALELL
LGRYPAGDIATAPTLPAMPPAPNASLPANVVERRPDLTAAAHRVAAAFDMRQSAEAARLPRISISAAVTGIRSDVFLLNQ
AGSPVKGLNGAFFAPIFMGGELKARVDYYTAEQKAALIAYGNSALRALGEVETGLRAESGYAERTRQLRERVNERRVLVQ
REETRVTIGASDTRSLQQQRQTLVAAEMDLVNVEADHLDQRVALLLALGGDWRPETTATATATATATGTGTGTGT

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=463, Percent_Identity=23.7580993520518, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50968; Mature: 50837

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSQIKKSLLLALPVAMLAGCALQTPPKGEDLRQQTVPSLEGRAGWANPTSGDAVADGW
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHCCH
LAAFDDAELQRLVREAIAHNGDLRLAEARVQQAQALVAIARSGLLPSAGVKGRAGNSETQ
HHHCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
ILSIGASWEVDLWGRIRAESRSAESQYAATQDDYLWAQRVIAATTAKAWFSLIRYTVLED
EEEECCCEEEEHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLRQSATIQEELVRIVGQRVTIGIAPETDLLEARNTLHAQRDGIEKTALARSQAAQALEL
HHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LLGRYPAGDIATAPTLPAMPPAPNASLPANVVERRPDLTAAAHRVAAAFDMRQSAEAARL
HHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
PRISISAAVTGIRSDVFLLNQAGSPVKGLNGAFFAPIFMGGELKARVDYYTAEQKAALIA
CCEEHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEHEEECCCEEEEEEEEECCCCEEEEE
YGNSALRALGEVETGLRAESGYAERTRQLRERVNERRVLVQREETRVTIGASDTRSLQQQ
ECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCEEEECCCHHHHHHHH
RQTLVAAEMDLVNVEADHLDQRVALLLALGGDWRPETTATATATATATGTGTGTGT
HHHHHHHHHHHEECCHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
SSSQIKKSLLLALPVAMLAGCALQTPPKGEDLRQQTVPSLEGRAGWANPTSGDAVADGW
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHCCH
LAAFDDAELQRLVREAIAHNGDLRLAEARVQQAQALVAIARSGLLPSAGVKGRAGNSETQ
HHHCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
ILSIGASWEVDLWGRIRAESRSAESQYAATQDDYLWAQRVIAATTAKAWFSLIRYTVLED
EEEECCCEEEEHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLRQSATIQEELVRIVGQRVTIGIAPETDLLEARNTLHAQRDGIEKTALARSQAAQALEL
HHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LLGRYPAGDIATAPTLPAMPPAPNASLPANVVERRPDLTAAAHRVAAAFDMRQSAEAARL
HHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
PRISISAAVTGIRSDVFLLNQAGSPVKGLNGAFFAPIFMGGELKARVDYYTAEQKAALIA
CCEEHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEHEEECCCEEEEEEEEECCCCEEEEE
YGNSALRALGEVETGLRAESGYAERTRQLRERVNERRVLVQREETRVTIGASDTRSLQQQ
ECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCEEEECCCHHHHHHHH
RQTLVAAEMDLVNVEADHLDQRVALLLALGGDWRPETTATATATATATGTGTGTGT
HHHHHHHHHHHEECCHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]