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The map label for this gene is metR [H]

Identifier: 94313485

GI number: 94313485

Start: 1171754

End: 1172677

Strand: Reverse

Name: metR [H]

Synonym: Rmet_4563

Alternate gene names: 94313485

Gene position: 1172677-1171754 (Counterclockwise)

Preceding gene: 94313488

Following gene: 94313483

Centisome position: 45.45

GC content: 65.48

Gene sequence:

>924_bases
ATGATTGAAGTCCGCCATTTCCGCTCGCTCGTGGCCATCGCCGAATCGGGCAAGCTGGCAACGGCCGCTGAACGCGTGCA
CGTGAGCCAGTCGGCGCTCTCCCATCAGATCAAGGCCATCGAGGCGCACTATGACATGCCGCTGTTCGATCGCACGCGCC
AGGGATTGCGTTTCACGCCCGCAGGGGAACGCCTGCTGGCCCTGGCACGCGAGGCGTTGGCGGCGATCAGCGCGGCCGAG
CGCGATCTGCTGCGCCTCAAGGGCGATACGCGCGGGGAGTTGCGCGTGGTACTCGAATGCCACACCTGTTTCGACTGGCT
GATGCCTGTGATGGACGAGTTCCGTCGCCGCTGGCCCGAGGTGGAGGTGGATCTGGTTGCCGGATTCCACGCAGACCCGC
TGGCACTGTTGAACAACGGCAAGGCGGATATGGTGATCGGATCGAAGCCGCCGGTAAAGCGGGGGCTGACCGTGGCGCCG
TTGTTCCGCTTCGAGATTCTGGCGGTGATGGCCAACGAGCATCGGCTGCGCAGCAAGCGGCGCATCGAGGCACAGGACCT
GGCGGGAGAAACGCTGATTACCTATCCCGTGCCGGAGCAGCGGATCGATCTGATCCGCGAGGTGCTGGAGCCGGCCGGTA
TTCGTCTGCAGCGGCGCACCGCGGAGCTTACGGTGGCGGTGCTGCAACTCGTGGCCAGCCGGCGTGGCGTCGCCGCGCTA
CCGAACTGGGGCGTGAAGAACTACGTCGATCACGACTACGTGCTGGCCAAACGCATCGGCACGAAGGGGCTGTGGAGCGA
GCTATACGCGACGGTGCCGGCCGCCATCGCCGACCGGCCCTATGTGGACGATTTCGTGTCGATCGTGCGCGATATCTGCG
CGGCCGAACTCGACGGGATTGAACTGCTGGGGACGCCGGCCTAG

Upstream 100 bases:

>100_bases
TCTGATATGGCGCACAGTTTCGGATTGCGGGTAAAATGAATCAAGCGATAAGTTCTAAACAAAGAGTGAATGAAATTCAT
ATCAAAAACGAGGCTGGGCA

Downstream 100 bases:

>100_bases
CGGCTCAGGCTCAGGCTCAGGCGTGGGCGGCCTCGGCTTCGACCTCAACGCAACCCACCAGAAGCATGTCGACGTCATCA
GAGAACTCGGCGTCGACAAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MIEVRHFRSLVAIAESGKLATAAERVHVSQSALSHQIKAIEAHYDMPLFDRTRQGLRFTPAGERLLALAREALAAISAAE
RDLLRLKGDTRGELRVVLECHTCFDWLMPVMDEFRRRWPEVEVDLVAGFHADPLALLNNGKADMVIGSKPPVKRGLTVAP
LFRFEILAVMANEHRLRSKRRIEAQDLAGETLITYPVPEQRIDLIREVLEPAGIRLQRRTAELTVAVLQLVASRRGVAAL
PNWGVKNYVDHDYVLAKRIGTKGLWSELYATVPAAIADRPYVDDFVSIVRDICAAELDGIELLGTPA

Sequences:

>Translated_307_residues
MIEVRHFRSLVAIAESGKLATAAERVHVSQSALSHQIKAIEAHYDMPLFDRTRQGLRFTPAGERLLALAREALAAISAAE
RDLLRLKGDTRGELRVVLECHTCFDWLMPVMDEFRRRWPEVEVDLVAGFHADPLALLNNGKADMVIGSKPPVKRGLTVAP
LFRFEILAVMANEHRLRSKRRIEAQDLAGETLITYPVPEQRIDLIREVLEPAGIRLQRRTAELTVAVLQLVASRRGVAAL
PNWGVKNYVDHDYVLAKRIGTKGLWSELYATVPAAIADRPYVDDFVSIVRDICAAELDGIELLGTPA
>Mature_307_residues
MIEVRHFRSLVAIAESGKLATAAERVHVSQSALSHQIKAIEAHYDMPLFDRTRQGLRFTPAGERLLALAREALAAISAAE
RDLLRLKGDTRGELRVVLECHTCFDWLMPVMDEFRRRWPEVEVDLVAGFHADPLALLNNGKADMVIGSKPPVKRGLTVAP
LFRFEILAVMANEHRLRSKRRIEAQDLAGETLITYPVPEQRIDLIREVLEPAGIRLQRRTAELTVAVLQLVASRRGVAAL
PNWGVKNYVDHDYVLAKRIGTKGLWSELYATVPAAIADRPYVDDFVSIVRDICAAELDGIELLGTPA

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=291, Percent_Identity=40.5498281786942, Blast_Score=227, Evalue=7e-61,
Organism=Escherichia coli, GI157672245, Length=213, Percent_Identity=29.5774647887324, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790208, Length=149, Percent_Identity=30.8724832214765, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786401, Length=153, Percent_Identity=30.718954248366, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081904, Length=177, Percent_Identity=32.7683615819209, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787879, Length=195, Percent_Identity=28.7179487179487, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788887, Length=303, Percent_Identity=25.4125412541254, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790399, Length=194, Percent_Identity=29.8969072164948, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693105, Length=241, Percent_Identity=23.6514522821577, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787530, Length=198, Percent_Identity=24.2424242424242, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082024, Length=169, Percent_Identity=24.8520710059172, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI2367136, Length=218, Percent_Identity=29.3577981651376, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788296, Length=197, Percent_Identity=29.4416243654822, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787601, Length=147, Percent_Identity=25.1700680272109, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34264; Mature: 34264

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEVRHFRSLVAIAESGKLATAAERVHVSQSALSHQIKAIEAHYDMPLFDRTRQGLRFTP
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEECC
AGERLLALAREALAAISAAERDLLRLKGDTRGELRVVLECHTCFDWLMPVMDEFRRRWPE
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCC
VEVDLVAGFHADPLALLNNGKADMVIGSKPPVKRGLTVAPLFRFEILAVMANEHRLRSKR
CEEEEEECCCCCCHHHHCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
RIEAQDLAGETLITYPVPEQRIDLIREVLEPAGIRLQRRTAELTVAVLQLVASRRGVAAL
CCCHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCC
PNWGVKNYVDHDYVLAKRIGTKGLWSELYATVPAAIADRPYVDDFVSIVRDICAAELDGI
CCCCCHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
ELLGTPA
EECCCCC
>Mature Secondary Structure
MIEVRHFRSLVAIAESGKLATAAERVHVSQSALSHQIKAIEAHYDMPLFDRTRQGLRFTP
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEECC
AGERLLALAREALAAISAAERDLLRLKGDTRGELRVVLECHTCFDWLMPVMDEFRRRWPE
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCC
VEVDLVAGFHADPLALLNNGKADMVIGSKPPVKRGLTVAPLFRFEILAVMANEHRLRSKR
CEEEEEECCCCCCHHHHCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
RIEAQDLAGETLITYPVPEQRIDLIREVLEPAGIRLQRRTAELTVAVLQLVASRRGVAAL
CCCHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCC
PNWGVKNYVDHDYVLAKRIGTKGLWSELYATVPAAIADRPYVDDFVSIVRDICAAELDGI
CCCCCHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
ELLGTPA
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]