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The map label for this gene is mdtN [H]

Identifier: 94313435

GI number: 94313435

Start: 1112348

End: 1113397

Strand: Direct

Name: mdtN [H]

Synonym: Rmet_4510

Alternate gene names: 94313435

Gene position: 1112348-1113397 (Clockwise)

Preceding gene: 94313434

Following gene: 94313436

Centisome position: 43.11

GC content: 67.43

Gene sequence:

>1050_bases
ATGAAAATGGCCGGCGTTCAAAAGGGGCCGCTGCGCGGCCGGGTCATTACCCTGGCCATCGTGCTGGTGGCCGTGTTGCT
TTCCGTCTATGCCTATGAGCGCACCGTGCACCATCCGTCGTCGGACGATGCCACGCTCGACGCCGATGTCGTTCATGTGG
CCTCGCCGGTAGGCGGACGCATCGCCCGCATCGCCGTCACCGAGAACCAGCACGTAGCCAAGGGCGACGTGCTATTCGAA
ATCGACCCTGTGCCTTACAAGCTGGCGGTGGACCAGGCGCGGGCAGACCTCGAACTCGCGCAGGCGGGCCTCGGCACGCG
GCGGCGCACGATCGTCAGCGAACGCGCGGCCGCGTCGATCGCCTCCGAGCAGACCACGCGCGCGGCGCAAAACTACGCCT
TGACGGCACGCACGGTGGAACGCCTGCGCCCGCTGGCCGCCGATGGCTACGTGCCAAAGCAGCAGCTCGACCAGGCCCAG
GTGGCGCAGCATGACGCGGCGGTCTCGCTCAAGCAGGCGAACGTCCAGCACTCGGCATCCACGCAAGCCATCGGCAACGA
CGACGACGCCATCGCCGCGGTCCACGCCCGGGAAGCCGCGCTCGCAATCGCCCAGCGCGCGTTGCAGGACACCGTAGTCC
ATGCCCCGCAGGAAGGCCGCGTCACCGGGCTCTCCGTGCTGGCCGGTGAAGTCGTGATTCCAAACCAGTCGCTGTTCCTG
CTCGTTCATACCGGCGAATGGTTTGCCGTGGCGAACTTCCGGGAGACATCGCTAGCCGATATTCATATCGGCGACTGCGC
CACGGTCTACTCGATGATCGACCGGCGCAAGGCGATACGCGGCACCGTGACCGGCATCGGCGCGGGCATCACCGATACGG
AACGGCTCAACCTGCCGCGCTCGCTGCCCTACGTGCAGGCCTCGGTGAACTGGGTACGCGTCGCCAAGCGTTTCCCGGTT
CGCATCCGGCTCGAAGATCCGCCCGAGACGCTGGCCCGCGCCGGCGCCAGCGCAAACGTCGAGATCCGGCATGGCGCAGC
CTGCCGCTGA

Upstream 100 bases:

>100_bases
CCGGTTGGGCGGAAACTGTTCGTTCCCAATTAATTGTCAATACAGCAATTGGCCTGATCATCGCCGAGATCGTCTGGCTG
ATCGGTACGGGGCATCTCCT

Downstream 100 bases:

>100_bases
CAAGCTGCGCCTCGGCCTGGGCGACATCGGCGCCCTGCTCGCGCCGACGCCCGGCCGGGCGGCCGCCACCACCCGTATCA
CCGTGGCGAGCGTGCTGACG

Product: multidrug resistance protein MdtN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKMAGVQKGPLRGRVITLAIVLVAVLLSVYAYERTVHHPSSDDATLDADVVHVASPVGGRIARIAVTENQHVAKGDVLFE
IDPVPYKLAVDQARADLELAQAGLGTRRRTIVSERAAASIASEQTTRAAQNYALTARTVERLRPLAADGYVPKQQLDQAQ
VAQHDAAVSLKQANVQHSASTQAIGNDDDAIAAVHAREAALAIAQRALQDTVVHAPQEGRVTGLSVLAGEVVIPNQSLFL
LVHTGEWFAVANFRETSLADIHIGDCATVYSMIDRRKAIRGTVTGIGAGITDTERLNLPRSLPYVQASVNWVRVAKRFPV
RIRLEDPPETLARAGASANVEIRHGAACR

Sequences:

>Translated_349_residues
MKMAGVQKGPLRGRVITLAIVLVAVLLSVYAYERTVHHPSSDDATLDADVVHVASPVGGRIARIAVTENQHVAKGDVLFE
IDPVPYKLAVDQARADLELAQAGLGTRRRTIVSERAAASIASEQTTRAAQNYALTARTVERLRPLAADGYVPKQQLDQAQ
VAQHDAAVSLKQANVQHSASTQAIGNDDDAIAAVHAREAALAIAQRALQDTVVHAPQEGRVTGLSVLAGEVVIPNQSLFL
LVHTGEWFAVANFRETSLADIHIGDCATVYSMIDRRKAIRGTVTGIGAGITDTERLNLPRSLPYVQASVNWVRVAKRFPV
RIRLEDPPETLARAGASANVEIRHGAACR
>Mature_349_residues
MKMAGVQKGPLRGRVITLAIVLVAVLLSVYAYERTVHHPSSDDATLDADVVHVASPVGGRIARIAVTENQHVAKGDVLFE
IDPVPYKLAVDQARADLELAQAGLGTRRRTIVSERAAASIASEQTTRAAQNYALTARTVERLRPLAADGYVPKQQLDQAQ
VAQHDAAVSLKQANVQHSASTQAIGNDDDAIAAVHAREAALAIAQRALQDTVVHAPQEGRVTGLSVLAGEVVIPNQSLFL
LVHTGEWFAVANFRETSLADIHIGDCATVYSMIDRRKAIRGTVTGIGAGITDTERLNLPRSLPYVQASVNWVRVAKRFPV
RIRLEDPPETLARAGASANVEIRHGAACR

Specific function: Could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790519, Length=316, Percent_Identity=36.3924050632911, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789637, Length=325, Percent_Identity=27.0769230769231, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081951, Length=332, Percent_Identity=27.1084337349398, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1788711, Length=322, Percent_Identity=27.9503105590062, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789041, Length=316, Percent_Identity=26.8987341772152, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790024, Length=285, Percent_Identity=25.9649122807018, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37428; Mature: 37428

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMAGVQKGPLRGRVITLAIVLVAVLLSVYAYERTVHHPSSDDATLDADVVHVASPVGGR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEECCCCCCE
IARIAVTENQHVAKGDVLFEIDPVPYKLAVDQARADLELAQAGLGTRRRTIVSERAAASI
EEEEEEECCCCEECCCEEEEECCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ASEQTTRAAQNYALTARTVERLRPLAADGYVPKQQLDQAQVAQHDAAVSLKQANVQHSAS
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHEEHHHHCCCCCCC
TQAIGNDDDAIAAVHAREAALAIAQRALQDTVVHAPQEGRVTGLSVLAGEVVIPNQSLFL
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECEEEECCCCEEE
LVHTGEWFAVANFRETSLADIHIGDCATVYSMIDRRKAIRGTVTGIGAGITDTERLNLPR
EEECCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHCCCCC
SLPYVQASVNWVRVAKRFPVRIRLEDPPETLARAGASANVEIRHGAACR
CCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCCEEECCCCCCC
>Mature Secondary Structure
MKMAGVQKGPLRGRVITLAIVLVAVLLSVYAYERTVHHPSSDDATLDADVVHVASPVGGR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEECCCCCCE
IARIAVTENQHVAKGDVLFEIDPVPYKLAVDQARADLELAQAGLGTRRRTIVSERAAASI
EEEEEEECCCCEECCCEEEEECCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ASEQTTRAAQNYALTARTVERLRPLAADGYVPKQQLDQAQVAQHDAAVSLKQANVQHSAS
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHEEHHHHCCCCCCC
TQAIGNDDDAIAAVHAREAALAIAQRALQDTVVHAPQEGRVTGLSVLAGEVVIPNQSLFL
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECEEEECCCCEEE
LVHTGEWFAVANFRETSLADIHIGDCATVYSMIDRRKAIRGTVTGIGAGITDTERLNLPR
EEECCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHCCCCC
SLPYVQASVNWVRVAKRFPVRIRLEDPPETLARAGASANVEIRHGAACR
CCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]