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The map label for this gene is livM3 [H]

Identifier: 94313402

GI number: 94313402

Start: 1077323

End: 1078321

Strand: Direct

Name: livM3 [H]

Synonym: Rmet_4477

Alternate gene names: 94313402

Gene position: 1077323-1078321 (Clockwise)

Preceding gene: 94313401

Following gene: 94313403

Centisome position: 41.76

GC content: 64.16

Gene sequence:

>999_bases
ATGGCGTCAAGCAGACAATGGTTGTTTCGCTACCGCTACTGGTGGCTAGCGCTGGGCGTCACGCTGCTGCTCCCGTTGAC
GATGCGTTCGGGCACGCTGGCGACCGAAGTCCTGATCTATGGCATGGCTGCCATGGCCTGCAATCTTCTGCTCGGGTATA
CGGGTCTGCTGTCGTTCGGCCAGGGGATCTTCTTTGGATTGGGAAGCTATGCGGTTGGCCTGGCGCTGACACGTGCCGGC
GGCCTGCCGATGCCCGTGGCCCTGCTGGGTGCCGTGGTGATCGGCGCGGCGACCGCAGCCCTGGTAGGGTGGTTCTCCAT
CCGTCAACGCGGCACGTATTTCGTGATGCTGACGCTGGCGTTCGCGCAGATGTTCTACTTCCTCGCCTACACGGCGCCCG
GCATCACCGGCGGTGACAACGGCTTGCTCGACATCCCACGCCCCCCGATTTCCATCGGCGGGCATGCCCTGATCGATCTG
ACGTCGTCGTGGGGATTCTACGGATTCGTGGCGGTGCTGTTCCTGATCGTCTTCGCACTCGTTCTGCGCGTCACGGAATC
CGTCCTCGGCCGCACGCTGCTGGCCATTCGCGACAACGAGGAACGGGCGCTTGCCGTCGGCTACAACACCCGGGCCTTCA
AACTGCTGGCATTCGTGATCTCCGGTGCCGTGACGGGGCTTGCCGGCGCCTTGCACGCCATGCTGACAGGTATCGCGCCG
CTGGCGAACATCGACTACCACGCGAGCGAAATGATCCTGATCATGACGGTGATCGGCGGCACCAGCAATATCTTCGCCTC
GGTGCTCGGCGCGGCGTTCTACGTCCTGCTGGCGGACTGGCTGTCCACACTCTGGCCGCGCTGGCTCATGCTTCTGGGCT
TCCTGCTTATCGCGGTCAGCCTGTTCATGCAGCGCGGATTGTGGGGCCTGGGATCGACCATCGCGGCACGCCTGCGAGGT
GTGACGGGCGCCGCATCCACCCAGGAGGAGCGCGCATGA

Upstream 100 bases:

>100_bases
TGATGAGCACGCTCTGGCCGGAAGGTGCCCGGCTGATGATCTATGTGGCCATGGCCGCGGTGCTGTTGCTGCGTCCGCAC
GGCCTGCTTGGGAGGGGATG

Downstream 100 bases:

>100_bases
GCACGCCGATTCTTGAAGCCACAGGCATCGTCAAGCAATACGGGAAGTTCATGGCGCTGGGCGGGGTCGACCTGCGGGTG
ATGCCCGGGACCGTTCATTC

Product: Branched-chain amino acid inner-membrane translocator (inner-membrane translocator)

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MASSRQWLFRYRYWWLALGVTLLLPLTMRSGTLATEVLIYGMAAMACNLLLGYTGLLSFGQGIFFGLGSYAVGLALTRAG
GLPMPVALLGAVVIGAATAALVGWFSIRQRGTYFVMLTLAFAQMFYFLAYTAPGITGGDNGLLDIPRPPISIGGHALIDL
TSSWGFYGFVAVLFLIVFALVLRVTESVLGRTLLAIRDNEERALAVGYNTRAFKLLAFVISGAVTGLAGALHAMLTGIAP
LANIDYHASEMILIMTVIGGTSNIFASVLGAAFYVLLADWLSTLWPRWLMLLGFLLIAVSLFMQRGLWGLGSTIAARLRG
VTGAASTQEERA

Sequences:

>Translated_332_residues
MASSRQWLFRYRYWWLALGVTLLLPLTMRSGTLATEVLIYGMAAMACNLLLGYTGLLSFGQGIFFGLGSYAVGLALTRAG
GLPMPVALLGAVVIGAATAALVGWFSIRQRGTYFVMLTLAFAQMFYFLAYTAPGITGGDNGLLDIPRPPISIGGHALIDL
TSSWGFYGFVAVLFLIVFALVLRVTESVLGRTLLAIRDNEERALAVGYNTRAFKLLAFVISGAVTGLAGALHAMLTGIAP
LANIDYHASEMILIMTVIGGTSNIFASVLGAAFYVLLADWLSTLWPRWLMLLGFLLIAVSLFMQRGLWGLGSTIAARLRG
VTGAASTQEERA
>Mature_331_residues
ASSRQWLFRYRYWWLALGVTLLLPLTMRSGTLATEVLIYGMAAMACNLLLGYTGLLSFGQGIFFGLGSYAVGLALTRAGG
LPMPVALLGAVVIGAATAALVGWFSIRQRGTYFVMLTLAFAQMFYFLAYTAPGITGGDNGLLDIPRPPISIGGHALIDLT
SSWGFYGFVAVLFLIVFALVLRVTESVLGRTLLAIRDNEERALAVGYNTRAFKLLAFVISGAVTGLAGALHAMLTGIAPL
ANIDYHASEMILIMTVIGGTSNIFASVLGAAFYVLLADWLSTLWPRWLMLLGFLLIAVSLFMQRGLWGLGSTIAARLRGV
TGAASTQEERA

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=325, Percent_Identity=27.3846153846154, Blast_Score=95, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 35539; Mature: 35407

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSRQWLFRYRYWWLALGVTLLLPLTMRSGTLATEVLIYGMAAMACNLLLGYTGLLSFG
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QGIFFGLGSYAVGLALTRAGGLPMPVALLGAVVIGAATAALVGWFSIRQRGTYFVMLTLA
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FAQMFYFLAYTAPGITGGDNGLLDIPRPPISIGGHALIDLTSSWGFYGFVAVLFLIVFAL
HHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
VLRVTESVLGRTLLAIRDNEERALAVGYNTRAFKLLAFVISGAVTGLAGALHAMLTGIAP
HHHHHHHHHHHHHHEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LANIDYHASEMILIMTVIGGTSNIFASVLGAAFYVLLADWLSTLWPRWLMLLGFLLIAVS
HCCCCCCHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFMQRGLWGLGSTIAARLRGVTGAASTQEERA
HHHHHCCCCCHHHHHHHHHCCCCCCCCHHCCC
>Mature Secondary Structure 
ASSRQWLFRYRYWWLALGVTLLLPLTMRSGTLATEVLIYGMAAMACNLLLGYTGLLSFG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QGIFFGLGSYAVGLALTRAGGLPMPVALLGAVVIGAATAALVGWFSIRQRGTYFVMLTLA
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FAQMFYFLAYTAPGITGGDNGLLDIPRPPISIGGHALIDLTSSWGFYGFVAVLFLIVFAL
HHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
VLRVTESVLGRTLLAIRDNEERALAVGYNTRAFKLLAFVISGAVTGLAGALHAMLTGIAP
HHHHHHHHHHHHHHEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LANIDYHASEMILIMTVIGGTSNIFASVLGAAFYVLLADWLSTLWPRWLMLLGFLLIAVS
HCCCCCCHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFMQRGLWGLGSTIAARLRGVTGAASTQEERA
HHHHHCCCCCHHHHHHHHHCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]