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The map label for this gene is pcaR [H]

Identifier: 94313197

GI number: 94313197

Start: 862052

End: 862906

Strand: Reverse

Name: pcaR [H]

Synonym: Rmet_4271

Alternate gene names: 94313197

Gene position: 862906-862052 (Counterclockwise)

Preceding gene: 94313202

Following gene: 94313192

Centisome position: 33.44

GC content: 67.02

Gene sequence:

>855_bases
ATGACATCAACCGACCTGCCCCGCCCACCCATCAGTGAGGCACCCTCGCCCCGACCGGACGAGGGTCATCGTGACTACGT
AGCGGCTCTGGCGCGTGGACTGGCCGTCATCCAGGCGTTCACCGAGGGCTTGCCCGAGATGACCCTGAGCGAAGTCGCCC
AGGCCACAGGCATGACCCCGGCCACGGCACGCCGCGCATTGCTTACGCTGCAATCGCTCGGCTATGTCGGCGCCAACGGG
CGCCGCTTCCTGCTGCTGCCGAAAGTGCTGTCGCTCGGCGCGGCCTACCTCAGTTCGATGAACCTGCGCGATGTCGTCCA
ACCGTTCCTGCAAGAGGTGGCCGACCGCTTTCGCGACGCCGTCTCCCTGGCGATCCTGGATGGCGATAGCGTGGTCTACC
TGGCACACGTGCCAAGCAGCCGACGCATCGTGTTCCGGGCATCAGTCGGCTACCGCCTGCCCGTGCATTGCACTTCGCTC
GGTCTTACCCTGCTGGCCCACGCTCCGCAGGCACAGCAGGACGCGTTGCTAGCCAAGGCGCCGTTCCAGAAGTACACCCC
GATGACGCTCAGTGAAGCGGACCAACTGCGCACGGCCCTGGAACAGGTTCGCGAGCAGGGCTACGCGCTGCAGGAAAGCC
AGCTGGAGATTGGCGTGCTGTCGGTCGCGGTGCCGGTGCGGGATGCTTCCCGCCGCGTCATCGCCGCGGTCAACTGCTCC
ACCGAATCCGGGCGAGCCGACACGCAAAACCTGCTGTCCGAACGGCTGCCGGCGCTACGCGAGGCTGCCGCCATGATCGA
ATCCGCGCTGCTGCGGGCCCCAGCGCTGGCGCACTCCATCACCGCTGCTGGCTAG

Upstream 100 bases:

>100_bases
TTGCTGTCGGACATGTTTCCGGCTTGGGTCATTCAGCTACCTCCCACAACTGTCTCGTTCGTAAAAAGGGCATCCGCCCA
AGTGCGGATAAAATGCGGTC

Downstream 100 bases:

>100_bases
ACCACGGGCTCCATTGCCACGTCCAGCGTGATGAGCTGATGACCGTCGCGCTCGGGATGTGGGGTGACTTCGACGATATG
GTCGGCGATGGTCGAGAAGA

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTSTDLPRPPISEAPSPRPDEGHRDYVAALARGLAVIQAFTEGLPEMTLSEVAQATGMTPATARRALLTLQSLGYVGANG
RRFLLLPKVLSLGAAYLSSMNLRDVVQPFLQEVADRFRDAVSLAILDGDSVVYLAHVPSSRRIVFRASVGYRLPVHCTSL
GLTLLAHAPQAQQDALLAKAPFQKYTPMTLSEADQLRTALEQVREQGYALQESQLEIGVLSVAVPVRDASRRVIAAVNCS
TESGRADTQNLLSERLPALREAAAMIESALLRAPALAHSITAAG

Sequences:

>Translated_284_residues
MTSTDLPRPPISEAPSPRPDEGHRDYVAALARGLAVIQAFTEGLPEMTLSEVAQATGMTPATARRALLTLQSLGYVGANG
RRFLLLPKVLSLGAAYLSSMNLRDVVQPFLQEVADRFRDAVSLAILDGDSVVYLAHVPSSRRIVFRASVGYRLPVHCTSL
GLTLLAHAPQAQQDALLAKAPFQKYTPMTLSEADQLRTALEQVREQGYALQESQLEIGVLSVAVPVRDASRRVIAAVNCS
TESGRADTQNLLSERLPALREAAAMIESALLRAPALAHSITAAG
>Mature_283_residues
TSTDLPRPPISEAPSPRPDEGHRDYVAALARGLAVIQAFTEGLPEMTLSEVAQATGMTPATARRALLTLQSLGYVGANGR
RFLLLPKVLSLGAAYLSSMNLRDVVQPFLQEVADRFRDAVSLAILDGDSVVYLAHVPSSRRIVFRASVGYRLPVHCTSLG
LTLLAHAPQAQQDALLAKAPFQKYTPMTLSEADQLRTALEQVREQGYALQESQLEIGVLSVAVPVRDASRRVIAAVNCST
ESGRADTQNLLSERLPALREAAAMIESALLRAPALAHSITAAG

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=246, Percent_Identity=32.9268292682927, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1788131, Length=272, Percent_Identity=27.9411764705882, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI87082359, Length=237, Percent_Identity=26.5822784810127, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786715, Length=244, Percent_Identity=25.8196721311475, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789999, Length=254, Percent_Identity=29.1338582677165, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1788581, Length=156, Percent_Identity=30.1282051282051, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693096, Length=230, Percent_Identity=30.4347826086957, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 30445; Mature: 30314

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTDLPRPPISEAPSPRPDEGHRDYVAALARGLAVIQAFTEGLPEMTLSEVAQATGMTP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
ATARRALLTLQSLGYVGANGRRFLLLPKVLSLGAAYLSSMNLRDVVQPFLQEVADRFRDA
HHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VSLAILDGDSVVYLAHVPSSRRIVFRASVGYRLPVHCTSLGLTLLAHAPQAQQDALLAKA
HEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHC
PFQKYTPMTLSEADQLRTALEQVREQGYALQESQLEIGVLSVAVPVRDASRRVIAAVNCS
CHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCEEEEEEECC
TESGRADTQNLLSERLPALREAAAMIESALLRAPALAHSITAAG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSTDLPRPPISEAPSPRPDEGHRDYVAALARGLAVIQAFTEGLPEMTLSEVAQATGMTP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
ATARRALLTLQSLGYVGANGRRFLLLPKVLSLGAAYLSSMNLRDVVQPFLQEVADRFRDA
HHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VSLAILDGDSVVYLAHVPSSRRIVFRASVGYRLPVHCTSLGLTLLAHAPQAQQDALLAKA
HEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHC
PFQKYTPMTLSEADQLRTALEQVREQGYALQESQLEIGVLSVAVPVRDASRRVIAAVNCS
CHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCEEEEEEECC
TESGRADTQNLLSERLPALREAAAMIESALLRAPALAHSITAAG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]