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The map label for this gene is pcA [H]

Identifier: 94313196

GI number: 94313196

Start: 861222

End: 862055

Strand: Direct

Name: pcA [H]

Synonym: Rmet_4270

Alternate gene names: 94313196

Gene position: 861222-862055 (Clockwise)

Preceding gene: 94313195

Following gene: 94313198

Centisome position: 33.38

GC content: 64.15

Gene sequence:

>834_bases
ATGGACCAGACCAACAACCCCACGCTATCGCGAGCATTGGCCGCAATGTGCACGGATGCCGCCGGCGCCGACTCGGCCTT
GCAGGAGAAGTTCGCAATCGCGCTTCGCCATTTCCATGCAATGCTCGACGAACTGCAGCTGACCGATGTGGAGCTCTACC
GCATCGCTGCCTGGCTTGGGCAGGTGGCGCGAGACGATGAGGTGATCATGCTGTGCGACATGATGGGCCTGACCATGCGT
TCGCTGGACCTGGCCCGCACCGGCAGCGAAGCGACCGCACAGAACGTGACCGGTCCGTTCCCGAAGGACGGCATTGCCGA
AGGCCCGAACCCCTGCCAGCTTGCCGCCGATGACGAACCCGGCCAACGGCTCGAATTGAGGGGGCGGGTGAGGGATGCGG
TGAGCGGCCGCGTGCTGCCTGGCGCGGAGGTTATCGTCTGGCAGCCCAACCAACTCGGTCGCTATGAGAACGAGGACGAC
TCACAGTCCGACGACAACCTGCGCGGCAAGCTGCGTTGCGATACGGAGGGCAGCTTCCGGGTGCTCACTATCCGGCTGGG
CGGCTACATCATCGGCCGCGAGGATACGGCGGTGGGAGTGCTGATGAAGCGGCTTGGTCGCAACCGGCAGCGTGCGCCCC
ACATCCACTACCGCGTGCTGCATCCGGGCTACCAGACACTGACCTCGCAGGTCTACTTCGCCGGGGAACCAGCCAATTCG
GTCGACTGCATCTTCTCGACCATCGCCGACCATATCGTCGAAGTCACCCCACATCCCGAGCGCGACGGTCATCAGCTCAT
CACGCTGGACGTGGCAATGGAGCCCGTGGTCTAG

Upstream 100 bases:

>100_bases
AGACGCGTGCCTGCTGGCGCGCGCGACGGATGTTGCCGGCTGAGCGGAACCCGTATGACGGCTGCCACAAGCCGCAACCC
TGACAAAAATGGAGACAACG

Downstream 100 bases:

>100_bases
CCAGCAGCGGTGATGGAGTGCGCCAGCGCTGGGGCCCGCAGCAGCGCGGATTCGATCATGGCGGCAGCCTCGCGTAGCGC
CGGCAGCCGTTCGGACAGCA

Product: Protocatechuate 3,4-dioxygenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MDQTNNPTLSRALAAMCTDAAGADSALQEKFAIALRHFHAMLDELQLTDVELYRIAAWLGQVARDDEVIMLCDMMGLTMR
SLDLARTGSEATAQNVTGPFPKDGIAEGPNPCQLAADDEPGQRLELRGRVRDAVSGRVLPGAEVIVWQPNQLGRYENEDD
SQSDDNLRGKLRCDTEGSFRVLTIRLGGYIIGREDTAVGVLMKRLGRNRQRAPHIHYRVLHPGYQTLTSQVYFAGEPANS
VDCIFSTIADHIVEVTPHPERDGHQLITLDVAMEPVV

Sequences:

>Translated_277_residues
MDQTNNPTLSRALAAMCTDAAGADSALQEKFAIALRHFHAMLDELQLTDVELYRIAAWLGQVARDDEVIMLCDMMGLTMR
SLDLARTGSEATAQNVTGPFPKDGIAEGPNPCQLAADDEPGQRLELRGRVRDAVSGRVLPGAEVIVWQPNQLGRYENEDD
SQSDDNLRGKLRCDTEGSFRVLTIRLGGYIIGREDTAVGVLMKRLGRNRQRAPHIHYRVLHPGYQTLTSQVYFAGEPANS
VDCIFSTIADHIVEVTPHPERDGHQLITLDVAMEPVV
>Mature_277_residues
MDQTNNPTLSRALAAMCTDAAGADSALQEKFAIALRHFHAMLDELQLTDVELYRIAAWLGQVARDDEVIMLCDMMGLTMR
SLDLARTGSEATAQNVTGPFPKDGIAEGPNPCQLAADDEPGQRLELRGRVRDAVSGRVLPGAEVIVWQPNQLGRYENEDD
SQSDDNLRGKLRCDTEGSFRVLTIRLGGYIIGREDTAVGVLMKRLGRNRQRAPHIHYRVLHPGYQTLTSQVYFAGEPANS
VDCIFSTIADHIVEVTPHPERDGHQLITLDVAMEPVV

Specific function: Preferentially converts 3,5-dichlorocatechol as opposed to other chlorinated catechols. Retains diminished activity toward non-chlorinated substrates [H]

COG id: COG3485

COG function: function code Q; Protocatechuate 3,4-dioxygenase beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the intradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007535
- InterPro:   IPR012817
- InterPro:   IPR000627
- InterPro:   IPR015889 [H]

Pfam domain/function: PF00775 Dioxygenase_C; PF04444 Dioxygenase_N [H]

EC number: NA

Molecular weight: Translated: 30546; Mature: 30546

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQTNNPTLSRALAAMCTDAAGADSALQEKFAIALRHFHAMLDELQLTDVELYRIAAWLG
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QVARDDEVIMLCDMMGLTMRSLDLARTGSEATAQNVTGPFPKDGIAEGPNPCQLAADDEP
HHCCCCCEEEEECHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCEECCCCCC
GQRLELRGRVRDAVSGRVLPGAEVIVWQPNQLGRYENEDDSQSDDNLRGKLRCDTEGSFR
CCEEHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEE
VLTIRLGGYIIGREDTAVGVLMKRLGRNRQRAPHIHYRVLHPGYQTLTSQVYFAGEPANS
EEEEEECCEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHEEEEECCCCCH
VDCIFSTIADHIVEVTPHPERDGHQLITLDVAMEPVV
HHHHHHHHHHHHEECCCCCCCCCCEEEEEEEECCCCC
>Mature Secondary Structure
MDQTNNPTLSRALAAMCTDAAGADSALQEKFAIALRHFHAMLDELQLTDVELYRIAAWLG
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QVARDDEVIMLCDMMGLTMRSLDLARTGSEATAQNVTGPFPKDGIAEGPNPCQLAADDEP
HHCCCCCEEEEECHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCEECCCCCC
GQRLELRGRVRDAVSGRVLPGAEVIVWQPNQLGRYENEDDSQSDDNLRGKLRCDTEGSFR
CCEEHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEE
VLTIRLGGYIIGREDTAVGVLMKRLGRNRQRAPHIHYRVLHPGYQTLTSQVYFAGEPANS
EEEEEECCEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHEEEEECCCCCH
VDCIFSTIADHIVEVTPHPERDGHQLITLDVAMEPVV
HHHHHHHHHHHHEECCCCCCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7509586 [H]