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The map label for this gene is citH [H]

Identifier: 94313183

GI number: 94313183

Start: 848982

End: 849674

Strand: Direct

Name: citH [H]

Synonym: Rmet_4257

Alternate gene names: 94313183

Gene position: 848982-849674 (Clockwise)

Preceding gene: 94313181

Following gene: 94313184

Centisome position: 32.91

GC content: 63.35

Gene sequence:

>693_bases
GTGATTCCCGTTGGTCTCTATATCCGCCGGCATCTCGATGAAACCGGCGTCGTGCAGGAGGGCGGCGCGCCGAGGCAGGA
GCACGCCGGCCTGCGCGAGATCTTCACCCGCTATTGGCGCATGGTGGTGGCCGGAGTACTGATCACCATCGGTGGCACCT
CGGCCACCTATATCGTGCTGCACTACATGACCAGCTACGCCATCTCGGTGCTGAAGATCCCGGCCGGGGTCAGCATGGCG
GCAGGCTGTGTGGGCGCACTGGTGCAGGTGGCACTGTCTGCGTACGCGGGCAGGTTGTCGGACCGGATCGGCCGCAAACC
GCTTATCCTCTGGTCGCGTATCGCGATGCTGGTCGCGATCTACCCGGGCTTCCTGCTGCTCAATGCTCAGCCGACCCTGC
CCGTGCTGCTGGGCGTTGTTGCCGCGCTGTCACTGATGCTGGTGCTGAACGTCGTGCCGTCAGTGGTAATGCTCTCGGAA
CTGTTTCCGCGGCGCATCCGTGCGACTGGCATGTCGATCGTGTACTGCATTGGCGTGCTGGTGTTTGGTGGTTTCGCGCA
GTTCCTGGCCACCTGGTTGATCGCCGTGACCGGCAATGCGAATGCCCCCGCGCAATATGTGATCGGTTGCGGCATCGTAT
CGCTTGTGGGCCTGGCCATGGTCGCGGAAACGGCCGGCAAGCGACTGAACTGA

Upstream 100 bases:

>100_bases
CGACATGGGTGCCTTGATCGGACTGACGCTGACCAACACCCTGTCACCGGAAGCGCTCGAAAGCTGGGGTTGGCGCATCC
CTTTTGCGATCGGCATCCTG

Downstream 100 bases:

>100_bases
GCGACCGCGATCCAAGCGGCAGCATCTCTGGCGTCAAGCGACGTGCAAAGCGTTGTCTTATTGCGATCGCCCGAGCCTCG
CAGCGAGCAGCCTGTTCGCG

Product: proline/glycine betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MIPVGLYIRRHLDETGVVQEGGAPRQEHAGLREIFTRYWRMVVAGVLITIGGTSATYIVLHYMTSYAISVLKIPAGVSMA
AGCVGALVQVALSAYAGRLSDRIGRKPLILWSRIAMLVAIYPGFLLLNAQPTLPVLLGVVAALSLMLVLNVVPSVVMLSE
LFPRRIRATGMSIVYCIGVLVFGGFAQFLATWLIAVTGNANAPAQYVIGCGIVSLVGLAMVAETAGKRLN

Sequences:

>Translated_230_residues
MIPVGLYIRRHLDETGVVQEGGAPRQEHAGLREIFTRYWRMVVAGVLITIGGTSATYIVLHYMTSYAISVLKIPAGVSMA
AGCVGALVQVALSAYAGRLSDRIGRKPLILWSRIAMLVAIYPGFLLLNAQPTLPVLLGVVAALSLMLVLNVVPSVVMLSE
LFPRRIRATGMSIVYCIGVLVFGGFAQFLATWLIAVTGNANAPAQYVIGCGIVSLVGLAMVAETAGKRLN
>Mature_230_residues
MIPVGLYIRRHLDETGVVQEGGAPRQEHAGLREIFTRYWRMVVAGVLITIGGTSATYIVLHYMTSYAISVLKIPAGVSMA
AGCVGALVQVALSAYAGRLSDRIGRKPLILWSRIAMLVAIYPGFLLLNAQPTLPVLLGVVAALSLMLVLNVVPSVVMLSE
LFPRRIRATGMSIVYCIGVLVFGGFAQFLATWLIAVTGNANAPAQYVIGCGIVSLVGLAMVAETAGKRLN

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=244, Percent_Identity=29.9180327868852, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788942, Length=234, Percent_Identity=27.7777777777778, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 24469; Mature: 24469

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVGLYIRRHLDETGVVQEGGAPRQEHAGLREIFTRYWRMVVAGVLITIGGTSATYIVL
CCCHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
HYMTSYAISVLKIPAGVSMAAGCVGALVQVALSAYAGRLSDRIGRKPLILWSRIAMLVAI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YPGFLLLNAQPTLPVLLGVVAALSLMLVLNVVPSVVMLSELFPRRIRATGMSIVYCIGVL
HCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFGGFAQFLATWLIAVTGNANAPAQYVIGCGIVSLVGLAMVAETAGKRLN
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIPVGLYIRRHLDETGVVQEGGAPRQEHAGLREIFTRYWRMVVAGVLITIGGTSATYIVL
CCCHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
HYMTSYAISVLKIPAGVSMAAGCVGALVQVALSAYAGRLSDRIGRKPLILWSRIAMLVAI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YPGFLLLNAQPTLPVLLGVVAALSLMLVLNVVPSVVMLSELFPRRIRATGMSIVYCIGVL
HCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFGGFAQFLATWLIAVTGNANAPAQYVIGCGIVSLVGLAMVAETAGKRLN
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]