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The map label for this gene is hsaD [H]

Identifier: 94313147

GI number: 94313147

Start: 804285

End: 805181

Strand: Direct

Name: hsaD [H]

Synonym: Rmet_4220

Alternate gene names: 94313147

Gene position: 804285-805181 (Clockwise)

Preceding gene: 94313146

Following gene: 94313148

Centisome position: 31.17

GC content: 64.55

Gene sequence:

>897_bases
ATGACGAGCCCGATATCTACAACGACCCCACGGCCCGAGACGCCGGACGAGATCCTTGAGCGGCTCAGCGCCAGGGCAAT
CGTCCACCGGGACCGATCCGGTCCCACAGAGATGGTCTGGCGCGAGATCGGTCGTGGCTCCCCCCTGGTACTGATCCATG
GCGGTCATGGAAACTGGATGCACTGGGTGCGCAATATCGAGGCGCTGAGCCAGCAACACACGCTATGGCTGCCTGACCTG
CCCGGTTTCGGTGAATCTGGCACGCTCGACGGCGATCCTCACGCGGATGACCGGCTGGATCGGCTGGTCGATACAGTGGC
AGCCAGTCTGAGACAGCGGTTCGGCGCGTCGGATGGATTCGCGCTGGCGGGCTTCTCTTTCGGTGGTGTAGTCGCGGCCC
GCCTGGCGGCCGCGCTGCCGAATGTCGAGAAGCTTGCGCTGCTGGGCTCCGCCGGGCATGGCGGTATGCGGCGCCAGCGA
CTCGATATGGTCAACTGGCGCACGGAGGACCGCGAGCAGATGCTGCAAGGCTTGAGGCACAACCTCAAGGCACTGATGCT
TCACGATCCGTCCAGCATTGATGCGCTGGCCATGGCGGTGCACGAACGGGCTTGCATGGCCACGCGCTTTCGCAGCAAGT
CCATTTCGCGCGGCGGCCCGGTGCTCGGACAGGCGCTCGACAGCTATGGCGGAAAGACGCTGCTGATCTGGGGCGAGCAT
GACGTGACGGCTGACCCGTTGGAAGTGGCACCGCGCATCGCGGGAAACTTGTCGACTCGGCGATGGCACCTGGTTCCCGG
CGGCGGGCATTGGGTGCAATACGAACGAGCGGACGAAATCAATCAGTTGCTGCTGCAGTGGTTCGCCAGTGGCGACGGCG
GATTGCACGCCCGATGA

Upstream 100 bases:

>100_bases
GCGAATCGAGGCGATTTTTTCTCGTCCATGACTTGCTTCGGGCACGCCGTGCGTGGTCAGATACAGCGCTTCATTTCCCA
CGTCCCCACGTATTTGCGAT

Downstream 100 bases:

>100_bases
GCGGCGGCCTTGTCGGCGGCATGTTCGATGCCGGCAAGGCGCGAATCAGCCCGATTCGTCTTATCGTTTCTCTTTCCATA
TCAACTCCATGGGTTCGTTT

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase; HOPDA hydrolase; Meta-cleavage product hydrolase; MCP hydrolase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTSPISTTTPRPETPDEILERLSARAIVHRDRSGPTEMVWREIGRGSPLVLIHGGHGNWMHWVRNIEALSQQHTLWLPDL
PGFGESGTLDGDPHADDRLDRLVDTVAASLRQRFGASDGFALAGFSFGGVVAARLAAALPNVEKLALLGSAGHGGMRRQR
LDMVNWRTEDREQMLQGLRHNLKALMLHDPSSIDALAMAVHERACMATRFRSKSISRGGPVLGQALDSYGGKTLLIWGEH
DVTADPLEVAPRIAGNLSTRRWHLVPGGGHWVQYERADEINQLLLQWFASGDGGLHAR

Sequences:

>Translated_298_residues
MTSPISTTTPRPETPDEILERLSARAIVHRDRSGPTEMVWREIGRGSPLVLIHGGHGNWMHWVRNIEALSQQHTLWLPDL
PGFGESGTLDGDPHADDRLDRLVDTVAASLRQRFGASDGFALAGFSFGGVVAARLAAALPNVEKLALLGSAGHGGMRRQR
LDMVNWRTEDREQMLQGLRHNLKALMLHDPSSIDALAMAVHERACMATRFRSKSISRGGPVLGQALDSYGGKTLLIWGEH
DVTADPLEVAPRIAGNLSTRRWHLVPGGGHWVQYERADEINQLLLQWFASGDGGLHAR
>Mature_297_residues
TSPISTTTPRPETPDEILERLSARAIVHRDRSGPTEMVWREIGRGSPLVLIHGGHGNWMHWVRNIEALSQQHTLWLPDLP
GFGESGTLDGDPHADDRLDRLVDTVAASLRQRFGASDGFALAGFSFGGVVAARLAAALPNVEKLALLGSAGHGGMRRQRL
DMVNWRTEDREQMLQGLRHNLKALMLHDPSSIDALAMAVHERACMATRFRSKSISRGGPVLGQALDSYGGKTLLIWGEHD
VTADPLEVAPRIAGNLSTRRWHLVPGGGHWVQYERADEINQLLLQWFASGDGGLHAR

Specific function: Catalyzes the hydrolysis of a carbon-carbon bond in 4,5- 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA) to yield 9,17-dioxo-1,2,3,4,10,19- hexanorandrostan-5-oic acid (DOHNAA) and 2-hydroxy-hexa-2,4- dienoic acid (HHD). Is

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. HsaD family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=270, Percent_Identity=28.1481481481481, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.7.1.8 [H]

Molecular weight: Translated: 32720; Mature: 32588

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPISTTTPRPETPDEILERLSARAIVHRDRSGPTEMVWREIGRGSPLVLIHGGHGNWM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCCHH
HWVRNIEALSQQHTLWLPDLPGFGESGTLDGDPHADDRLDRLVDTVAASLRQRFGASDGF
HHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
ALAGFSFGGVVAARLAAALPNVEKLALLGSAGHGGMRRQRLDMVNWRTEDREQMLQGLRH
EEECCCHHHHHHHHHHHHCCCHHHHHEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
NLKALMLHDPSSIDALAMAVHERACMATRFRSKSISRGGPVLGQALDSYGGKTLLIWGEH
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCC
DVTADPLEVAPRIAGNLSTRRWHLVPGGGHWVQYERADEINQLLLQWFASGDGGLHAR
CCCCCHHHHHHHHHCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TSPISTTTPRPETPDEILERLSARAIVHRDRSGPTEMVWREIGRGSPLVLIHGGHGNWM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCCHH
HWVRNIEALSQQHTLWLPDLPGFGESGTLDGDPHADDRLDRLVDTVAASLRQRFGASDGF
HHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
ALAGFSFGGVVAARLAAALPNVEKLALLGSAGHGGMRRQRLDMVNWRTEDREQMLQGLRH
EEECCCHHHHHHHHHHHHCCCHHHHHEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
NLKALMLHDPSSIDALAMAVHERACMATRFRSKSISRGGPVLGQALDSYGGKTLLIWGEH
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCC
DVTADPLEVAPRIAGNLSTRRWHLVPGGGHWVQYERADEINQLLLQWFASGDGGLHAR
CCCCCHHHHHHHHHCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]