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The map label for this gene is rhtB [C]

Identifier: 94313145

GI number: 94313145

Start: 802504

End: 803142

Strand: Reverse

Name: rhtB [C]

Synonym: Rmet_4218

Alternate gene names: 94313145

Gene position: 803142-802504 (Counterclockwise)

Preceding gene: 94313154

Following gene: 94313143

Centisome position: 31.13

GC content: 65.57

Gene sequence:

>639_bases
ATGAGAACGATGTACGAATTCATGGCCGTGGGCGGCCTGCTGGCGATCACGCCGGGCCCCAATATGGTCTACGTGATGGC
GCGCTCGGTCTCGCAAGGCACCCGGGCTGGGCTGACTTCACTGGCGGGCGTCATGCTCGGTTACATGACCTACATGCTCG
GCGCGGCATTTGGCATCAGTGCGCTGTTCCTGGCGATTCCTGGCGCCGCGCGCCTGCTTGCCATCGCCGGTGCTGCCTAC
CTGCTCTACATGGCCTGGCAGATGCTCAAGCCAGGCGGCCGGTCCCCGCTTCAGATCCAGGCAACCCAGACCGAACGGCC
GCGTCGCCTGTTTACGATGGGCGCGATGACCAGCCTGCTCAATCCCAAGCTCGCGCTAGTCTTCCTGTCGATACTGCCGC
AATTCATCGACTATCAGGCTGGCGGCGTCCTCAGGCAATCGTTGGTCCTGGGGCTGGCCCTGACCGCCGCTTTCGCATCG
GTCAACGCGCTCGTCGCCATATCGTCGGGCCGCGCAGCGGCAATGATGGGAAGCCACCCACGCTGGCTGCTGGCGCAGCG
CCTTGTGGCGGGCGGCGTGCTTCTGCTGCTCGGGGCAAGGATGGCGCTGGAAGCCGCGCACTGGCCGGCGCTGTTGTAG

Upstream 100 bases:

>100_bases
CTGAACAATCGGATCAGTATAATCCGTTTTACTTAGTTATCCCGTGGTCCGATACTGCCAGCATTCCCGATCAAGGATGG
TTCTGGACCAGGTGCAGCGC

Downstream 100 bases:

>100_bases
CGCCATCACCCATTTACGGCGAGTTCATCCTCAAACTTCCCCGGATCGACGGCGGATGGACGATCCACGGCATCGTGGTA
TCCACGCCCGTATCGTCCAA

Product: L-lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MRTMYEFMAVGGLLAITPGPNMVYVMARSVSQGTRAGLTSLAGVMLGYMTYMLGAAFGISALFLAIPGAARLLAIAGAAY
LLYMAWQMLKPGGRSPLQIQATQTERPRRLFTMGAMTSLLNPKLALVFLSILPQFIDYQAGGVLRQSLVLGLALTAAFAS
VNALVAISSGRAAAMMGSHPRWLLAQRLVAGGVLLLLGARMALEAAHWPALL

Sequences:

>Translated_212_residues
MRTMYEFMAVGGLLAITPGPNMVYVMARSVSQGTRAGLTSLAGVMLGYMTYMLGAAFGISALFLAIPGAARLLAIAGAAY
LLYMAWQMLKPGGRSPLQIQATQTERPRRLFTMGAMTSLLNPKLALVFLSILPQFIDYQAGGVLRQSLVLGLALTAAFAS
VNALVAISSGRAAAMMGSHPRWLLAQRLVAGGVLLLLGARMALEAAHWPALL
>Mature_212_residues
MRTMYEFMAVGGLLAITPGPNMVYVMARSVSQGTRAGLTSLAGVMLGYMTYMLGAAFGISALFLAIPGAARLLAIAGAAY
LLYMAWQMLKPGGRSPLQIQATQTERPRRLFTMGAMTSLLNPKLALVFLSILPQFIDYQAGGVLRQSLVLGLALTAAFAS
VNALVAISSGRAAAMMGSHPRWLLAQRLVAGGVLLLLGARMALEAAHWPALL

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=200, Percent_Identity=28, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788099, Length=130, Percent_Identity=30, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22399; Mature: 22399

Theoretical pI: Translated: 11.61; Mature: 11.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
7.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
7.1 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTMYEFMAVGGLLAITPGPNMVYVMARSVSQGTRAGLTSLAGVMLGYMTYMLGAAFGIS
CCHHHHHHHHCCEEEECCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFLAIPGAARLLAIAGAAYLLYMAWQMLKPGGRSPLQIQATQTERPRRLFTMGAMTSLL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
NPKLALVFLSILPQFIDYQAGGVLRQSLVLGLALTAAFASVNALVAISSGRAAAMMGSHP
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEECCCCCC
RWLLAQRLVAGGVLLLLGARMALEAAHWPALL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRTMYEFMAVGGLLAITPGPNMVYVMARSVSQGTRAGLTSLAGVMLGYMTYMLGAAFGIS
CCHHHHHHHHCCEEEECCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFLAIPGAARLLAIAGAAYLLYMAWQMLKPGGRSPLQIQATQTERPRRLFTMGAMTSLL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
NPKLALVFLSILPQFIDYQAGGVLRQSLVLGLALTAAFASVNALVAISSGRAAAMMGSHP
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEECCCCCC
RWLLAQRLVAGGVLLLLGARMALEAAHWPALL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8169201; 10984043 [H]