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The map label for this gene is lpdA2 [H]

Identifier: 94313143

GI number: 94313143

Start: 799845

End: 801641

Strand: Reverse

Name: lpdA2 [H]

Synonym: Rmet_4216

Alternate gene names: 94313143

Gene position: 801641-799845 (Counterclockwise)

Preceding gene: 94313145

Following gene: 94313136

Centisome position: 31.07

GC content: 64.22

Gene sequence:

>1797_bases
ATGGCCGCGATCGAAGTGAAGGTGCCGCAGTTGTCCGAATCTGTCTCGGAAGCCACGCTGATGCAGTGGAAGAAGCAAGC
CGGCGAGGCCGTCAAACGCGACGAGATCCTGGTGGAACTGGAAACCGACAAGGTCACGCTGGAGGTGCCGAGTCCGGCCG
ACGGCGTACTGGCAAAGATCGTCCAGCCCGATGGCGCCACGGTCCATACGGACGACGTGATCGCGGTGGTCGACACCGAG
GGGAAAGCGGTCGCAGCAGCCTCACCCGTGGCCGCTGCAGCGCCGGCATCCGCCCCAGCGCCCGCACCGGCCCCTGCCCC
GGTAGCCGCCGTGGCATCAGGTGGCGCTGGTGCCCCGGCATCGGCCAAGGCCGACTTCGACGTGATCGTGATCGGCTCCG
GCCCGGGTGGATATATCGCCGCCATCCGTGCGGCCCAGCTAGGCAAGACGGTAGCCTGCATCGAGGAATGGAAAGACGAC
GCTGGCAAGCCACGGCTCGGCGGTACCTGCCTGAACGTCGGCTGCATTCCAAGCAAGGCGCTGCTCGCCTCGTCCGAGTA
CTTCGAGCAAGCGAAGCATGGGCTGGCCGAACATGGTGTGCAGGTCAAGGGCGTCACACTTGATCTGGCGCAGATGATCC
AGCGCAAGGCTGCAATCGTCGACAAGTTCACCGGTGGCGTGGAGTTCCTGTTTCGCAAGAACAAGGTCACCTGGATCAAG
GGCCATGGCAAGTTCAAGGGGCGGGCCGCGGATGGCGTGATTACCGTGGAGGCCAGCAACGGCGGCGAGACGACGTCGCA
TACGGCACGCAACGTCATCATCGCAACGGGTTCGAAGGCCCGCCATCTTTCGGGCGTGCCGGTCGACAACAAAATCGTCT
CCGACAACGAGGGCGCGCTGTCCTTCGATTCGGTGCCGAAAAAACTCGCAGTGATCGGCGCCGGGGTGATCGGTCTCGAA
CTCGGCTCGGTATGGCGACGGCTCGGGTCCGAAGTCACGCTGCTGGAAGCCCTGCCCACATTCCTCGGCGCGCTTGACGA
AGCCGTGGCCAGGGAAGCCGCCAAACAGTTCGGCAAGCAGGGGCTCACGATTCACCTTGGTGTCGACATCGGCAATATCG
AAGCCACCGCCAAGGGCGTCAGCATCGCCTACAAGGACAAGGACGGCGCCGAACAGAAGCTCGTCGCGGACCGGTTGATC
GTGTCGATCGGGCGCGTGCCGAACACCGACAATCTGGGTCTGGATGCCGTGGGCCTGGCCGCCGACGCGCGTGGCTTCAT
CCCCGTGGACGACCAGTGCCGCACCCCGGTGGCGGGCATCTACGCTATCGGCGATGTCGTGCGCGGCCCGATGCTCGCCC
ACAAGGCCGAGGACGAAGGCGTCATGGCCGCCGAGGTCATCGACGGCCAGAAGCCACACATCGACTACAACTGCATTCCG
TGGGTGATCTACACCGAGCCCGAAATCGCCTGGGTGGGCAAGTCAGAAGCCCAGTTGAAGGCGGAAGGCCGCGAGTTCCG
TTCCGGCCAGTTCCCAATGATGGCAAACGGCCGCGCGCTTGGGATCGGACATCCGGATGGCTTCATCAAGATGATCGCGG
ACGCAAAGACCGACGAAATCCTGGGCGTGCACATCATCTCCGCCAATGCCTCGGATCTGATCGCCGAGGCCGTGGTCGCC
ATGGAGTTCAAGGCGGCATCGGAGGACATCGGCATGATCTGCCACCCGCACCCATCACTGTCCGAGGTCATGCGCGAGGC
TGCGCTGGCGGTGCGAAAGCGGGCGCTCAATATGTAG

Upstream 100 bases:

>100_bases
TTGCGCTCGTCGAAGTATCCGCTGCGCCACTCGGCTGGCATCCTTGGCGTGCTCTCCTAGACTGGAGGCTTTCGTCACAA
GAGACCCGCAAGGAAAAACC

Downstream 100 bases:

>100_bases
TTGGCGCTATCGGTCGTATTGCTCGTATTGATCGTACTGGTCGTCTTTCCCAAGGTAGCGACCCAGGACCGACTGGAGCG
TCTCGTAGCTGACCGGCTTC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MAAIEVKVPQLSESVSEATLMQWKKQAGEAVKRDEILVELETDKVTLEVPSPADGVLAKIVQPDGATVHTDDVIAVVDTE
GKAVAAASPVAAAAPASAPAPAPAPAPVAAVASGGAGAPASAKADFDVIVIGSGPGGYIAAIRAAQLGKTVACIEEWKDD
AGKPRLGGTCLNVGCIPSKALLASSEYFEQAKHGLAEHGVQVKGVTLDLAQMIQRKAAIVDKFTGGVEFLFRKNKVTWIK
GHGKFKGRAADGVITVEASNGGETTSHTARNVIIATGSKARHLSGVPVDNKIVSDNEGALSFDSVPKKLAVIGAGVIGLE
LGSVWRRLGSEVTLLEALPTFLGALDEAVAREAAKQFGKQGLTIHLGVDIGNIEATAKGVSIAYKDKDGAEQKLVADRLI
VSIGRVPNTDNLGLDAVGLAADARGFIPVDDQCRTPVAGIYAIGDVVRGPMLAHKAEDEGVMAAEVIDGQKPHIDYNCIP
WVIYTEPEIAWVGKSEAQLKAEGREFRSGQFPMMANGRALGIGHPDGFIKMIADAKTDEILGVHIISANASDLIAEAVVA
MEFKAASEDIGMICHPHPSLSEVMREAALAVRKRALNM

Sequences:

>Translated_598_residues
MAAIEVKVPQLSESVSEATLMQWKKQAGEAVKRDEILVELETDKVTLEVPSPADGVLAKIVQPDGATVHTDDVIAVVDTE
GKAVAAASPVAAAAPASAPAPAPAPAPVAAVASGGAGAPASAKADFDVIVIGSGPGGYIAAIRAAQLGKTVACIEEWKDD
AGKPRLGGTCLNVGCIPSKALLASSEYFEQAKHGLAEHGVQVKGVTLDLAQMIQRKAAIVDKFTGGVEFLFRKNKVTWIK
GHGKFKGRAADGVITVEASNGGETTSHTARNVIIATGSKARHLSGVPVDNKIVSDNEGALSFDSVPKKLAVIGAGVIGLE
LGSVWRRLGSEVTLLEALPTFLGALDEAVAREAAKQFGKQGLTIHLGVDIGNIEATAKGVSIAYKDKDGAEQKLVADRLI
VSIGRVPNTDNLGLDAVGLAADARGFIPVDDQCRTPVAGIYAIGDVVRGPMLAHKAEDEGVMAAEVIDGQKPHIDYNCIP
WVIYTEPEIAWVGKSEAQLKAEGREFRSGQFPMMANGRALGIGHPDGFIKMIADAKTDEILGVHIISANASDLIAEAVVA
MEFKAASEDIGMICHPHPSLSEVMREAALAVRKRALNM
>Mature_597_residues
AAIEVKVPQLSESVSEATLMQWKKQAGEAVKRDEILVELETDKVTLEVPSPADGVLAKIVQPDGATVHTDDVIAVVDTEG
KAVAAASPVAAAAPASAPAPAPAPAPVAAVASGGAGAPASAKADFDVIVIGSGPGGYIAAIRAAQLGKTVACIEEWKDDA
GKPRLGGTCLNVGCIPSKALLASSEYFEQAKHGLAEHGVQVKGVTLDLAQMIQRKAAIVDKFTGGVEFLFRKNKVTWIKG
HGKFKGRAADGVITVEASNGGETTSHTARNVIIATGSKARHLSGVPVDNKIVSDNEGALSFDSVPKKLAVIGAGVIGLEL
GSVWRRLGSEVTLLEALPTFLGALDEAVAREAAKQFGKQGLTIHLGVDIGNIEATAKGVSIAYKDKDGAEQKLVADRLIV
SIGRVPNTDNLGLDAVGLAADARGFIPVDDQCRTPVAGIYAIGDVVRGPMLAHKAEDEGVMAAEVIDGQKPHIDYNCIPW
VIYTEPEIAWVGKSEAQLKAEGREFRSGQFPMMANGRALGIGHPDGFIKMIADAKTDEILGVHIISANASDLIAEAVVAM
EFKAASEDIGMICHPHPSLSEVMREAALAVRKRALNM

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=471, Percent_Identity=46.2845010615711, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI50301238, Length=473, Percent_Identity=27.6955602536998, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI148277065, Length=476, Percent_Identity=26.0504201680672, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI148277071, Length=476, Percent_Identity=26.0504201680672, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI33519430, Length=476, Percent_Identity=26.0504201680672, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI33519428, Length=476, Percent_Identity=26.0504201680672, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI33519426, Length=476, Percent_Identity=26.0504201680672, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI291045266, Length=437, Percent_Identity=26.3157894736842, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI22035672, Length=492, Percent_Identity=26.4227642276423, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI291045268, Length=481, Percent_Identity=25.5717255717256, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1786307, Length=462, Percent_Identity=38.0952380952381, Blast_Score=305, Evalue=8e-84,
Organism=Escherichia coli, GI87082354, Length=473, Percent_Identity=31.5010570824524, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1789915, Length=444, Percent_Identity=27.7027027027027, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87081717, Length=476, Percent_Identity=27.1008403361345, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1786946, Length=99, Percent_Identity=43.4343434343434, Blast_Score=86, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI32565766, Length=475, Percent_Identity=48, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17557007, Length=491, Percent_Identity=25.4582484725051, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71983419, Length=403, Percent_Identity=28.7841191066998, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71983429, Length=403, Percent_Identity=28.7841191066998, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=489, Percent_Identity=26.5848670756646, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321091, Length=477, Percent_Identity=42.7672955974843, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6325240, Length=479, Percent_Identity=30.8977035490605, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6325166, Length=476, Percent_Identity=26.6806722689076, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=47.9915433403806, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24640549, Length=494, Percent_Identity=29.3522267206478, Blast_Score=136, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24640551, Length=531, Percent_Identity=28.8135593220339, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24640553, Length=487, Percent_Identity=29.3634496919918, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI17737741, Length=498, Percent_Identity=26.5060240963855, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 62258; Mature: 62127

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIEVKVPQLSESVSEATLMQWKKQAGEAVKRDEILVELETDKVTLEVPSPADGVLAKI
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCCHHHEE
VQPDGATVHTDDVIAVVDTEGKAVAAASPVAAAAPASAPAPAPAPAPVAAVASGGAGAPA
ECCCCCEEECCCEEEEEECCCCEEEECCCCHHCCCCCCCCCCCCCCCHHEEECCCCCCCC
SAKADFDVIVIGSGPGGYIAAIRAAQLGKTVACIEEWKDDAGKPRLGGTCLNVGCIPSKA
CCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCHH
LLASSEYFEQAKHGLAEHGVQVKGVTLDLAQMIQRKAAIVDKFTGGVEFLFRKNKVTWIK
HHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEE
GHGKFKGRAADGVITVEASNGGETTSHTARNVIIATGSKARHLSGVPVDNKIVSDNEGAL
CCCCCCCEECCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEECCCCCCE
SFDSVPKKLAVIGAGVIGLELGSVWRRLGSEVTLLEALPTFLGALDEAVAREAAKQFGKQ
EHHHCCHHHHHHCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLTIHLGVDIGNIEATAKGVSIAYKDKDGAEQKLVADRLIVSIGRVPNTDNLGLDAVGLA
CCEEEEEEEECCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
ADARGFIPVDDQCRTPVAGIYAIGDVVRGPMLAHKAEDEGVMAAEVIDGQKPHIDYNCIP
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEECCCCCCEEEEEECCCCCCCCCCCCEE
WVIYTEPEIAWVGKSEAQLKAEGREFRSGQFPMMANGRALGIGHPDGFIKMIADAKTDEI
EEEEECCCEEEECCCCHHHHHCCCHHCCCCCCEEECCEEEECCCCCHHHHEEECCCCCCE
LGVHIISANASDLIAEAVVAMEFKAASEDIGMICHPHPSLSEVMREAALAVRKRALNM
EEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAIEVKVPQLSESVSEATLMQWKKQAGEAVKRDEILVELETDKVTLEVPSPADGVLAKI
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCCHHHEE
VQPDGATVHTDDVIAVVDTEGKAVAAASPVAAAAPASAPAPAPAPAPVAAVASGGAGAPA
ECCCCCEEECCCEEEEEECCCCEEEECCCCHHCCCCCCCCCCCCCCCHHEEECCCCCCCC
SAKADFDVIVIGSGPGGYIAAIRAAQLGKTVACIEEWKDDAGKPRLGGTCLNVGCIPSKA
CCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCHH
LLASSEYFEQAKHGLAEHGVQVKGVTLDLAQMIQRKAAIVDKFTGGVEFLFRKNKVTWIK
HHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEE
GHGKFKGRAADGVITVEASNGGETTSHTARNVIIATGSKARHLSGVPVDNKIVSDNEGAL
CCCCCCCEECCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEECCCCCCE
SFDSVPKKLAVIGAGVIGLELGSVWRRLGSEVTLLEALPTFLGALDEAVAREAAKQFGKQ
EHHHCCHHHHHHCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLTIHLGVDIGNIEATAKGVSIAYKDKDGAEQKLVADRLIVSIGRVPNTDNLGLDAVGLA
CCEEEEEEEECCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
ADARGFIPVDDQCRTPVAGIYAIGDVVRGPMLAHKAEDEGVMAAEVIDGQKPHIDYNCIP
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEECCCCCCEEEEEECCCCCCCCCCCCEE
WVIYTEPEIAWVGKSEAQLKAEGREFRSGQFPMMANGRALGIGHPDGFIKMIADAKTDEI
EEEEECCCEEEECCCCHHHHHCCCHHCCCCCCEEECCEEEECCCCCHHHHEEECCCCCCE
LGVHIISANASDLIAEAVVAMEFKAASEDIGMICHPHPSLSEVMREAALAVRKRALNM
EEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]