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The map label for this gene is estB [H]

Identifier: 94313109

GI number: 94313109

Start: 766703

End: 767374

Strand: Reverse

Name: estB [H]

Synonym: Rmet_4182

Alternate gene names: 94313109

Gene position: 767374-766703 (Counterclockwise)

Preceding gene: 94313110

Following gene: 94313107

Centisome position: 29.74

GC content: 65.77

Gene sequence:

>672_bases
ATGAGCGAACCGCTGCCCGCCATCGAAATTGAAACCGCGCCGAACCCGGCGTTCTCCGTGATCTGGATGCACGGGCTGGG
CGCCGATGGCAGCGACTTCGTTCCAGTCGTCCCGGAACTCGGATTGCCCGAAACCCTCGCCGTCCGGTTCATCTTCCCGA
ACGCCCCGGCGATACCCGTGACCTGCAACGGCGGCTACGTCATGCCGGCCTGGTATGACATCTACTCGCTCGATGAAGCG
GGCCGCCGTGCCGACGAACGAGGCATCCTGCAATCCCGCGATGCCATCCGCACGCTGATCGCACGCGAAAACGCGCGCGG
CGTGCCAACCAATCGCATCGTCCTGGCAGGGTTCTCGCAAGGTGGCGCGATCGCGTACACCACGGCCCTGACCCATCCCG
AAACGCTCGCCGGGGTGATTGCGCTGTCGACATACATCCCCTCGACCGACCTGATCGCATCCGAGGCTGCACCGGCCAAC
GCCGCCACGCCGATCCTTGCCGCCCACGGCACCGAGGACGGTGTCGTGCCGCTGATGCTCGGCACCCGCGCGCGCGACTG
GGTGGCCGCCGCTGGCCATCCGGTCACATGGCTGACCTACCGGATGCCGCATTCGGTCTGCATCGAGGAAATTGTCGATA
TCGGCCAGTGGTTGCAGGAGCGCTTTGTGTGA

Upstream 100 bases:

>100_bases
CTGACGCCGCGCCGGCGTGGCAACACCCGCATGGGCTCGTTCGGGCCTGTGCGATACTGGCCGCCTTCCCTAGCTTCGTG
CTGTTGCACTGGAACCGGCC

Downstream 100 bases:

>100_bases
GTGCCGCGACGATTGCCGCGCTAACATGGCGGCCGTCCGATCCAATCTGCGGTCCGAGGTACGTCCGTCGTTCCTGATAA
AGGAACCCCTGCGGTGCTTC

Product: carboxylesterase 2

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSEPLPAIEIETAPNPAFSVIWMHGLGADGSDFVPVVPELGLPETLAVRFIFPNAPAIPVTCNGGYVMPAWYDIYSLDEA
GRRADERGILQSRDAIRTLIARENARGVPTNRIVLAGFSQGGAIAYTTALTHPETLAGVIALSTYIPSTDLIASEAAPAN
AATPILAAHGTEDGVVPLMLGTRARDWVAAAGHPVTWLTYRMPHSVCIEEIVDIGQWLQERFV

Sequences:

>Translated_223_residues
MSEPLPAIEIETAPNPAFSVIWMHGLGADGSDFVPVVPELGLPETLAVRFIFPNAPAIPVTCNGGYVMPAWYDIYSLDEA
GRRADERGILQSRDAIRTLIARENARGVPTNRIVLAGFSQGGAIAYTTALTHPETLAGVIALSTYIPSTDLIASEAAPAN
AATPILAAHGTEDGVVPLMLGTRARDWVAAAGHPVTWLTYRMPHSVCIEEIVDIGQWLQERFV
>Mature_222_residues
SEPLPAIEIETAPNPAFSVIWMHGLGADGSDFVPVVPELGLPETLAVRFIFPNAPAIPVTCNGGYVMPAWYDIYSLDEAG
RRADERGILQSRDAIRTLIARENARGVPTNRIVLAGFSQGGAIAYTTALTHPETLAGVIALSTYIPSTDLIASEAAPANA
ATPILAAHGTEDGVVPLMLGTRARDWVAAAGHPVTWLTYRMPHSVCIEEIVDIGQWLQERFV

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=224, Percent_Identity=38.8392857142857, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI9966764, Length=225, Percent_Identity=36.8888888888889, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17508057, Length=202, Percent_Identity=37.1287128712871, Blast_Score=133, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6323147, Length=208, Percent_Identity=32.2115384615385, Blast_Score=126, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45553055, Length=206, Percent_Identity=36.8932038834951, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI45553057, Length=206, Percent_Identity=36.8932038834951, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21357257, Length=206, Percent_Identity=36.8932038834951, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI161078179, Length=210, Percent_Identity=30, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 23868; Mature: 23737

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPLPAIEIETAPNPAFSVIWMHGLGADGSDFVPVVPELGLPETLAVRFIFPNAPAIPV
CCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEE
TCNGGYVMPAWYDIYSLDEAGRRADERGILQSRDAIRTLIARENARGVPTNRIVLAGFSQ
EECCCEECCCEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
GGAIAYTTALTHPETLAGVIALSTYIPSTDLIASEAAPANAATPILAAHGTEDGVVPLML
CCEEEEEEECCCCHHHHHHHHHHHHCCCCHHEECCCCCCCCCCCEEEECCCCCCEEEEEE
GTRARDWVAAAGHPVTWLTYRMPHSVCIEEIVDIGQWLQERFV
CCCCCCHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEPLPAIEIETAPNPAFSVIWMHGLGADGSDFVPVVPELGLPETLAVRFIFPNAPAIPV
CCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEE
TCNGGYVMPAWYDIYSLDEAGRRADERGILQSRDAIRTLIARENARGVPTNRIVLAGFSQ
EECCCEECCCEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
GGAIAYTTALTHPETLAGVIALSTYIPSTDLIASEAAPANAATPILAAHGTEDGVVPLML
CCEEEEEEECCCCHHHHHHHHHHHHCCCCHHEECCCCCCCCCCCEEEECCCCCCEEEEEE
GTRARDWVAAAGHPVTWLTYRMPHSVCIEEIVDIGQWLQERFV
CCCCCCHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA