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The map label for this gene is kefF [H]

Identifier: 94312949

GI number: 94312949

Start: 591242

End: 592027

Strand: Reverse

Name: kefF [H]

Synonym: Rmet_4021

Alternate gene names: 94312949

Gene position: 592027-591242 (Counterclockwise)

Preceding gene: 94312951

Following gene: 94312945

Centisome position: 22.95

GC content: 62.09

Gene sequence:

>786_bases
ATGAACGTACTGCTTGTCTATGCTCACCCCGAACCGAAATCGCTGAATGGCTCGCTCAAGGACTTCGCCGTGCAACGGCT
GGAGGCAGCAGGCCACCAGGTGCGGGTCTCGGACCTTTATGCCATGCAATGGAAGGCGCCGCTCGATGCCGGCGACTCGC
TGGCACCTGCGAATGGCACGCCGTTTCATCCGTCGCTCGCTTCGAAGCACGCATTCGAGAACGGACTGCAAAGTCCTGAC
ATCGCACGCGAGCAGGAAAAGCTGAAGTGGGCCGACGCGGTAATCCTGCAGTTTCCGCTCTGGTGGTTCTCAATGCCCGC
CATCCTCAAGGGCTGGATCGAGCGCGTCTATGCCTTTGGCTTCGCCTACGGTGTGGGCGAGCATTCCGATTCGCGCTGGG
GCAATCGATATGGCGAGGGCACGCTTGCAGGCAAGCGCGCCATGCTGATGGTGACGACTGGGGGGTGGGAATCCCACTAC
GATGCGCGCGGGATCAACGGACCGATCGAGGACATCCTGTTCCCGATCCATCACGGCATCCTGTACTACCCGGGATTCGA
CGTGCTGCCGCCCTTCCTGGTGTTTCGGTCGGGCCGGATCGACGGCGATCGCTATGCCGCGGTGACCGAAGAACTTGGTC
AGCGGCTGGACACGCTGTTCACGACGGAACCCATCGCCTTCCGGCCACAGAACGGCGGCCAGTACGAGATTCCGTCACTG
ATCCTGCGGTCGGACATCTCACCCGGACAGACCGGATTCGCGGCGCACGTGGCGCTGGAGAACTAG

Upstream 100 bases:

>100_bases
TAATCATGGCAATACCTGGGATATTAGGAATTCATTTCCAATATAACTCAGGCGAACCTAATCTTGGTCCTGCCCCATCC
TCAGTCATCAGGACACCGAC

Downstream 100 bases:

>100_bases
GCCGCTGCGGCAGCCGTGGGCGTGTGGGCAGCCAGTTCGCGGTAATAACGCGTATGACGATAGAGCCCGTAGAGGCTGGT
TGCCGTGAAACCGAGCTGCT

Product: Ribosyldihydronicotinamide dehydrogenase (quinone)

Products: NAD(P); reduced acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MNVLLVYAHPEPKSLNGSLKDFAVQRLEAAGHQVRVSDLYAMQWKAPLDAGDSLAPANGTPFHPSLASKHAFENGLQSPD
IAREQEKLKWADAVILQFPLWWFSMPAILKGWIERVYAFGFAYGVGEHSDSRWGNRYGEGTLAGKRAMLMVTTGGWESHY
DARGINGPIEDILFPIHHGILYYPGFDVLPPFLVFRSGRIDGDRYAAVTEELGQRLDTLFTTEPIAFRPQNGGQYEIPSL
ILRSDISPGQTGFAAHVALEN

Sequences:

>Translated_261_residues
MNVLLVYAHPEPKSLNGSLKDFAVQRLEAAGHQVRVSDLYAMQWKAPLDAGDSLAPANGTPFHPSLASKHAFENGLQSPD
IAREQEKLKWADAVILQFPLWWFSMPAILKGWIERVYAFGFAYGVGEHSDSRWGNRYGEGTLAGKRAMLMVTTGGWESHY
DARGINGPIEDILFPIHHGILYYPGFDVLPPFLVFRSGRIDGDRYAAVTEELGQRLDTLFTTEPIAFRPQNGGQYEIPSL
ILRSDISPGQTGFAAHVALEN
>Mature_261_residues
MNVLLVYAHPEPKSLNGSLKDFAVQRLEAAGHQVRVSDLYAMQWKAPLDAGDSLAPANGTPFHPSLASKHAFENGLQSPD
IAREQEKLKWADAVILQFPLWWFSMPAILKGWIERVYAFGFAYGVGEHSDSRWGNRYGEGTLAGKRAMLMVTTGGWESHY
DARGINGPIEDILFPIHHGILYYPGFDVLPPFLVFRSGRIDGDRYAAVTEELGQRLDTLFTTEPIAFRPQNGGQYEIPSL
ILRSDISPGQTGFAAHVALEN

Specific function: Required for full activity of kefC [H]

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily [H]

Homologues:

Organism=Homo sapiens, GI156564357, Length=225, Percent_Identity=41.7777777777778, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI4505415, Length=232, Percent_Identity=39.6551724137931, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI70995396, Length=232, Percent_Identity=33.1896551724138, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI70995422, Length=232, Percent_Identity=31.0344827586207, Blast_Score=84, Evalue=9e-17,
Organism=Escherichia coli, GI1786231, Length=137, Percent_Identity=35.036496350365, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787129, Length=124, Percent_Identity=32.258064516129, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1789750, Length=175, Percent_Identity=31.4285714285714, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.- [C]

Molecular weight: Translated: 28969; Mature: 28969

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLLVYAHPEPKSLNGSLKDFAVQRLEAAGHQVRVSDLYAMQWKAPLDAGDSLAPANGT
CCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEHEEEEEECCCCCCCCCCCCCCCC
PFHPSLASKHAFENGLQSPDIAREQEKLKWADAVILQFPLWWFSMPAILKGWIERVYAFG
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAYGVGEHSDSRWGNRYGEGTLAGKRAMLMVTTGGWESHYDARGINGPIEDILFPIHHGI
HHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCE
LYYPGFDVLPPFLVFRSGRIDGDRYAAVTEELGQRLDTLFTTEPIAFRPQNGGQYEIPSL
EEECCHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHH
ILRSDISPGQTGFAAHVALEN
HHHCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MNVLLVYAHPEPKSLNGSLKDFAVQRLEAAGHQVRVSDLYAMQWKAPLDAGDSLAPANGT
CCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEHEEEEEECCCCCCCCCCCCCCCC
PFHPSLASKHAFENGLQSPDIAREQEKLKWADAVILQFPLWWFSMPAILKGWIERVYAFG
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAYGVGEHSDSRWGNRYGEGTLAGKRAMLMVTTGGWESHYDARGINGPIEDILFPIHHGI
HHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCE
LYYPGFDVLPPFLVFRSGRIDGDRYAAVTEELGQRLDTLFTTEPIAFRPQNGGQYEIPSL
EEECCHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHH
ILRSDISPGQTGFAAHVALEN
HHHCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; acceptor [C]

Specific reaction: NAD(P)H2 + acceptor = NAD(P) + reduced acceptor [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA