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The map label for this gene is pcaK [H]

Identifier: 94312939

GI number: 94312939

Start: 580928

End: 582307

Strand: Reverse

Name: pcaK [H]

Synonym: Rmet_4011

Alternate gene names: 94312939

Gene position: 582307-580928 (Counterclockwise)

Preceding gene: 94312940

Following gene: 94312938

Centisome position: 22.57

GC content: 66.3

Gene sequence:

>1380_bases
ATGTCCCATGCCCCGCACTCAGCGACCAGTACGCTAGACGTCCAGTCGCTCATCGACAGCCAGCGCTTCTCCGCGTATCA
ATGGCTCATCCTAGTCCTATGTTTCCTTATCGTCGCCGTAGATGGCTTTGACACAGCGGCCATGGGCTACGTTGCGCCGG
CCCTGGTACAGGATTGGGGCATTGAGAAATCGTCACTTGGCCCCGTCATGAGCGCCGCGCTGTTCGGCCTGGCCTTCGGC
GCGATCTTTGCGGGGCCGATGGCCGACCGCGTCGGCCGCAAGAAGGTGCTGGTGCTGTCCGTGTTCTTCTTCGGTGCCTG
CAGTCTTGCCACGGCATTTGCGCCGACGATCCGGTGGCTAACTGTCTTGCGATTCCTGACCGGCCTCGGCCTGGGCGCCG
CCATGCCCAACGCGGTAACGCTGATGTCCGAGTACGCGCCAGCGCGCCGCCGCGCGGTACTGGTCAACACGATGTTCTGC
GGCTTCCCGCTGGGCTCGGCCGCCGGCGGCTTCTTCGCGGCGGCGATCATCCCGCAGTTCGGCTGGCATAGCGTGCTGGT
CTTCGGTGGCGTAGTGCCGCTGGTACTTGCGGTGGTGCTGGTGACTACGCTGCCGGAGTCGGTCCGCTATATGGTCGTGC
GCCAGTACCCCGCCGACCAGATCCGGCAGGTGCTGGCGCGCGTGACCGGCGCCCGAGTGGATGGCGCAGGCGTCTTCACG
ATCAGCGAGGCCGCGCCCGCGTCGTCGTCGGCCATCGGCACTGTGCTGGCGCCGCGCTATCGACTGGGCTCGACCATGCT
GTGGCTTACCTACTTCATGGGACTGGTGATCTTCTACCTGCTGACGAGCTGGATGCCGACGCTGATGAAAGACGCCGGTT
TCTCACTGCAGCGTGCGTCCTTCCTGACCGCCCTGTTCCCACTTGGCGGCGGCATCGGCACCATCGCGGCTGGCTGGTTC
ATGGACCGCTTCAATCCGAACAAGGTCATCGCCATGACCTACGCCCTGACCGGCGTGCTGATCTATGCCGTGGGCCAGGT
CGCCAGCGGCGATCAGGTGGTGCTGGGCGTGTTGATCTTCCTGGCCGGCACCGCGATGAATGGGGCGCAGTCGTCGATGC
CGTCGCTGGCCGCCGCGTTCTATCCGACGCAAGGCCGCGCCACCGGCGTGGCATGGATGCTCGGCATCGGACGATTCGGC
GGCATCACCGGCGCACTGCTCGGCGCCGAACTGTTGCGCCTGCATCTGGGCTTCGACGTCATCTTCACGCTGCTGGCGAT
CCCCGCGTTCGCCGCCACTGCCGCGCTGGTCGCCAAGCATGTGGGCGCCCGGGGCGCGGCCCCCGTCGACACTGGCGGAG
CCATGGTGTCCGGGCACTGA

Upstream 100 bases:

>100_bases
ACAGTGGTTCGCCCTTAGAACAATAAAGGCGAAACGGCCGCGCCAAACGCCCGTCAGAAATGCGAGAGCGGTCGCCGACA
GATCCGAAACGGAGACAATC

Downstream 100 bases:

>100_bases
GACCGGCCGGCGCCGCCCAGGCGCCGGTGAAGTTGCCCCATACGTGCTACCGTTCGGAGGTACCAGTGTCCAGCGCGCGA
TCTTGGATCAGCAAACCTCC

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSHAPHSATSTLDVQSLIDSQRFSAYQWLILVLCFLIVAVDGFDTAAMGYVAPALVQDWGIEKSSLGPVMSAALFGLAFG
AIFAGPMADRVGRKKVLVLSVFFFGACSLATAFAPTIRWLTVLRFLTGLGLGAAMPNAVTLMSEYAPARRRAVLVNTMFC
GFPLGSAAGGFFAAAIIPQFGWHSVLVFGGVVPLVLAVVLVTTLPESVRYMVVRQYPADQIRQVLARVTGARVDGAGVFT
ISEAAPASSSAIGTVLAPRYRLGSTMLWLTYFMGLVIFYLLTSWMPTLMKDAGFSLQRASFLTALFPLGGGIGTIAAGWF
MDRFNPNKVIAMTYALTGVLIYAVGQVASGDQVVLGVLIFLAGTAMNGAQSSMPSLAAAFYPTQGRATGVAWMLGIGRFG
GITGALLGAELLRLHLGFDVIFTLLAIPAFAATAALVAKHVGARGAAPVDTGGAMVSGH

Sequences:

>Translated_459_residues
MSHAPHSATSTLDVQSLIDSQRFSAYQWLILVLCFLIVAVDGFDTAAMGYVAPALVQDWGIEKSSLGPVMSAALFGLAFG
AIFAGPMADRVGRKKVLVLSVFFFGACSLATAFAPTIRWLTVLRFLTGLGLGAAMPNAVTLMSEYAPARRRAVLVNTMFC
GFPLGSAAGGFFAAAIIPQFGWHSVLVFGGVVPLVLAVVLVTTLPESVRYMVVRQYPADQIRQVLARVTGARVDGAGVFT
ISEAAPASSSAIGTVLAPRYRLGSTMLWLTYFMGLVIFYLLTSWMPTLMKDAGFSLQRASFLTALFPLGGGIGTIAAGWF
MDRFNPNKVIAMTYALTGVLIYAVGQVASGDQVVLGVLIFLAGTAMNGAQSSMPSLAAAFYPTQGRATGVAWMLGIGRFG
GITGALLGAELLRLHLGFDVIFTLLAIPAFAATAALVAKHVGARGAAPVDTGGAMVSGH
>Mature_458_residues
SHAPHSATSTLDVQSLIDSQRFSAYQWLILVLCFLIVAVDGFDTAAMGYVAPALVQDWGIEKSSLGPVMSAALFGLAFGA
IFAGPMADRVGRKKVLVLSVFFFGACSLATAFAPTIRWLTVLRFLTGLGLGAAMPNAVTLMSEYAPARRRAVLVNTMFCG
FPLGSAAGGFFAAAIIPQFGWHSVLVFGGVVPLVLAVVLVTTLPESVRYMVVRQYPADQIRQVLARVTGARVDGAGVFTI
SEAAPASSSAIGTVLAPRYRLGSTMLWLTYFMGLVIFYLLTSWMPTLMKDAGFSLQRASFLTALFPLGGGIGTIAAGWFM
DRFNPNKVIAMTYALTGVLIYAVGQVASGDQVVLGVLIFLAGTAMNGAQSSMPSLAAAFYPTQGRATGVAWMLGIGRFGG
ITGALLGAELLRLHLGFDVIFTLLAIPAFAATAALVAKHVGARGAAPVDTGGAMVSGH

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=474, Percent_Identity=25.3164556962025, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI8923870, Length=408, Percent_Identity=26.9607843137255, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI7662270, Length=323, Percent_Identity=22.6006191950464, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI213021148, Length=173, Percent_Identity=27.7456647398844, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI216548223, Length=311, Percent_Identity=23.7942122186495, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI73695465, Length=233, Percent_Identity=27.8969957081545, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI87081723, Length=388, Percent_Identity=34.2783505154639, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87082231, Length=428, Percent_Identity=24.0654205607477, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87082159, Length=390, Percent_Identity=25.1282051282051, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788074, Length=372, Percent_Identity=26.6129032258064, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788068, Length=342, Percent_Identity=24.8538011695906, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87082404, Length=371, Percent_Identity=21.832884097035, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789152, Length=417, Percent_Identity=21.8225419664269, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789515, Length=324, Percent_Identity=23.1481481481481, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87082320, Length=301, Percent_Identity=22.2591362126246, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786229, Length=215, Percent_Identity=23.2558139534884, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=425, Percent_Identity=24.2352941176471, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19922874, Length=404, Percent_Identity=22.5247524752475, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24640198, Length=461, Percent_Identity=23.2104121475054, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24640196, Length=461, Percent_Identity=23.2104121475054, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24640200, Length=461, Percent_Identity=23.2104121475054, Blast_Score=93, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48071; Mature: 47940

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCC
>Mature Secondary Structure 
SHAPHSATSTLDVQSLIDSQRFSAYQWLILVLCFLIVAVDGFDTAAMGYVAPALVQDWG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC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HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]