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The map label for this gene is aroK [H]

Identifier: 94312938

GI number: 94312938

Start: 580294

End: 580884

Strand: Reverse

Name: aroK [H]

Synonym: Rmet_4010

Alternate gene names: 94312938

Gene position: 580884-580294 (Counterclockwise)

Preceding gene: 94312939

Following gene: 94312937

Centisome position: 22.51

GC content: 67.34

Gene sequence:

>591_bases
GTGCTACCGTTCGGAGGTACCAGTGTCCAGCGCGCGATCTTGGATCAGCAAACCTCCGGAGACAATCTCTTCTTCATCGG
CTTCATGGGCGCCGGCAAGACGACGATCGGGCGCGCCGTGGCGCACGAGCTCGGCCGCCCGTTCTTCGACACCGACCACG
AGATCGAGACGCGCTGCGGTGTGCGGATCGCCACGATCTTCGAGCTCGAGGGCGAGGAAGGCTTCCGCCAGCGCGAGTCG
CGCGTCCTGGATGAACTGACGCAGCGCAGCGGGATCGTGCTGGCCACCGGCGGGGGCGCGGTACTGCGTCCGGAGAACCG
CGCCATGCTGCGTGCGCGCGGCCAAGTGGTCTACCTCGACGCCTCGCTGCCCGAGATCTGGCGCCGCACGCGGCGCAACC
GCAACCGTCCGCTGCTGCAGACCGACAATCCGCGCGCGCGGCTCGAGGCGCTGTTTTGCGAACGCGACCCGCTGTACCGG
GAAATTGCCCACATCGTCATGCCAGCCCATGGTGGCAGCATTGCCCAGGCTGTCGCCAATGTCCTGGCACGGTTGGACCT
TGCGCCTCCCGATCCATTCCCCCACGCATGA

Upstream 100 bases:

>100_bases
GGCGCCCGGGGCGCGGCCCCCGTCGACACTGGCGGAGCCATGGTGTCCGGGCACTGAGACCGGCCGGCGCCGCCCAGGCG
CCGGTGAAGTTGCCCCATAC

Downstream 100 bases:

>100_bases
AGTGACCACTCATGACCAATAACTCAAGTGAGTTATTGGTCCGCAAGCTACTCTGGATGCACACTTGCCGGGGGTCAGTC
GACACGACCCTGAAACGACT

Product: shikimate kinase I

Products: NA

Alternate protein names: SK [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MLPFGGTSVQRAILDQQTSGDNLFFIGFMGAGKTTIGRAVAHELGRPFFDTDHEIETRCGVRIATIFELEGEEGFRQRES
RVLDELTQRSGIVLATGGGAVLRPENRAMLRARGQVVYLDASLPEIWRRTRRNRNRPLLQTDNPRARLEALFCERDPLYR
EIAHIVMPAHGGSIAQAVANVLARLDLAPPDPFPHA

Sequences:

>Translated_196_residues
MLPFGGTSVQRAILDQQTSGDNLFFIGFMGAGKTTIGRAVAHELGRPFFDTDHEIETRCGVRIATIFELEGEEGFRQRES
RVLDELTQRSGIVLATGGGAVLRPENRAMLRARGQVVYLDASLPEIWRRTRRNRNRPLLQTDNPRARLEALFCERDPLYR
EIAHIVMPAHGGSIAQAVANVLARLDLAPPDPFPHA
>Mature_196_residues
MLPFGGTSVQRAILDQQTSGDNLFFIGFMGAGKTTIGRAVAHELGRPFFDTDHEIETRCGVRIATIFELEGEEGFRQRES
RVLDELTQRSGIVLATGGGAVLRPENRAMLRARGQVVYLDASLPEIWRRTRRNRNRPLLQTDNPRARLEALFCERDPLYR
EIAHIVMPAHGGSIAQAVANVLARLDLAPPDPFPHA

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate [H]

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family [H]

Homologues:

Organism=Escherichia coli, GI87082255, Length=165, Percent_Identity=50.3030303030303, Blast_Score=163, Evalue=9e-42,
Organism=Escherichia coli, GI1786587, Length=150, Percent_Identity=40, Blast_Score=97, Evalue=5e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000623
- InterPro:   IPR023000 [H]

Pfam domain/function: PF01202 SKI [H]

EC number: =2.7.1.71 [H]

Molecular weight: Translated: 21811; Mature: 21811

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPFGGTSVQRAILDQQTSGDNLFFIGFMGAGKTTIGRAVAHELGRPFFDTDHEIETRCG
CCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHCC
VRIATIFELEGEEGFRQRESRVLDELTQRSGIVLATGGGAVLRPENRAMLRARGQVVYLD
EEEEEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEECCCEEECCCCHHHHEECCCEEEEE
ASLPEIWRRTRRNRNRPLLQTDNPRARLEALFCERDPLYREIAHIVMPAHGGSIAQAVAN
CCCHHHHHHHHHCCCCCCEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHH
VLARLDLAPPDPFPHA
HHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLPFGGTSVQRAILDQQTSGDNLFFIGFMGAGKTTIGRAVAHELGRPFFDTDHEIETRCG
CCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHCC
VRIATIFELEGEEGFRQRESRVLDELTQRSGIVLATGGGAVLRPENRAMLRARGQVVYLD
EEEEEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEECCCEEECCCCHHHHEECCCEEEEE
ASLPEIWRRTRRNRNRPLLQTDNPRARLEALFCERDPLYREIAHIVMPAHGGSIAQAVAN
CCCHHHHHHHHHCCCCCCEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHH
VLARLDLAPPDPFPHA
HHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]