BacMap

Definition	Cupriavidus metallidurans CH34 megaplasmid, complete sequence.
Accession	NC_007974
Length	2,580,084

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The map label for this gene is livF4 [H]

Identifier: 94312905

GI number: 94312905

Start: 543940

End: 544647

Strand: Reverse

Name: livF4 [H]

Synonym: Rmet_3977

Alternate gene names: 94312905

Gene position: 544647-543940 (Counterclockwise)

Preceding gene: 94312906

Following gene: 94312904

Centisome position: 21.11

GC content: 63.84

Gene sequence:

>708_bases
ATGTTGCAGCTTGATCGCGTCTCGCTGTCGTACGGCAGCTTCCGCGCCCTGGACAATATCTCGCTGCACGCAGGCGCCGG
AGAACTCGTGGTACTGCTTGGCGCGAACGGCGCGGGCAAGAGCTCCATCTTCCTGGCGATGAGCGGCATCCATCGCATCA
GCGGCGGCAGCATGCGTTTCGACGGCCGCGAGCTTAGCGGGATGAAACCGTCGCAAATCGTGCAGGCGGGACTCGTGCAC
TGCCCAGAGGGCCGCAAGCTGTTCCCCGCAATGAGCGTCGAGAAGAACCTGACGCTTGGCGCGTATGTGCATCGCCGTGA
CCGCGCCGGCATCCGCAAGACGCTCGAAGAGGTCTACGAGATGTTCCCGATCCTGTTCCAGAAGAAGGACGATCCCGCGG
GGTCGCTGTCTGGCGGGCAACAGCAAATGGTGGCACTGGGCCGCGCGCTGATGAGCCGCCCGCGCGCGCTGCTGCTCGAC
GAGCCGTCGCTGGGTCTTGCACCGCTGGTGGTCAAGCAGATGTTCGAGATCATCCAGCGCATCAACCGCGCTGGCACCAC
CGTGCTGCTGGCGGAACAGAACGCCTACGCGGCGCTCGGCATCGCCCATCGCGCCTACGTGATCGAGAACGGTCACATCG
TGATGGAAGGTGATCGCGATACGCTGCTGAAGGATGAGGGGATCAGGAAGGCGTACATCGGCGGGTAA

Upstream 100 bases:

>100_bases
AAGATCATGGTGCTCAACTACGGCGAAAAGATCGCCGAAGGCACGCCGGCGCAGATCCAGCAAGATCCGCACGTCATCGA
GGCCTACCTGGGAGCCGACC

Downstream 100 bases:

>100_bases
TTCGATTCGCCGAACCGAGTAGATACACCCTTCTCTCACTCGCGGGAAAAGGGCGATAAGGGCGTAGAGAACAAGCGCAG
TCAATACTCAAAGGAGACAG

Product: branched-chain amino acid ABC transporter ATP-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein F [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MLQLDRVSLSYGSFRALDNISLHAGAGELVVLLGANGAGKSSIFLAMSGIHRISGGSMRFDGRELSGMKPSQIVQAGLVH
CPEGRKLFPAMSVEKNLTLGAYVHRRDRAGIRKTLEEVYEMFPILFQKKDDPAGSLSGGQQQMVALGRALMSRPRALLLD
EPSLGLAPLVVKQMFEIIQRINRAGTTVLLAEQNAYAALGIAHRAYVIENGHIVMEGDRDTLLKDEGIRKAYIGG

Sequences:

>Translated_235_residues
MLQLDRVSLSYGSFRALDNISLHAGAGELVVLLGANGAGKSSIFLAMSGIHRISGGSMRFDGRELSGMKPSQIVQAGLVH
CPEGRKLFPAMSVEKNLTLGAYVHRRDRAGIRKTLEEVYEMFPILFQKKDDPAGSLSGGQQQMVALGRALMSRPRALLLD
EPSLGLAPLVVKQMFEIIQRINRAGTTVLLAEQNAYAALGIAHRAYVIENGHIVMEGDRDTLLKDEGIRKAYIGG
>Mature_235_residues
MLQLDRVSLSYGSFRALDNISLHAGAGELVVLLGANGAGKSSIFLAMSGIHRISGGSMRFDGRELSGMKPSQIVQAGLVH
CPEGRKLFPAMSVEKNLTLGAYVHRRDRAGIRKTLEEVYEMFPILFQKKDDPAGSLSGGQQQMVALGRALMSRPRALLLD
EPSLGLAPLVVKQMFEIIQRINRAGTTVLLAEQNAYAALGIAHRAYVIENGHIVMEGDRDTLLKDEGIRKAYIGG

Specific function: Component of the leucine-specific transport system [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI45446740, Length=221, Percent_Identity=28.0542986425339, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI47078218, Length=221, Percent_Identity=28.0542986425339, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI27436953, Length=207, Percent_Identity=27.0531400966184, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI27477115, Length=218, Percent_Identity=28.8990825688073, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI153792144, Length=218, Percent_Identity=26.605504587156, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI27881501, Length=214, Percent_Identity=25.2336448598131, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI30795238, Length=214, Percent_Identity=25.2336448598131, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI31657092, Length=219, Percent_Identity=25.5707762557078, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI27262624, Length=226, Percent_Identity=26.9911504424779, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI27262626, Length=226, Percent_Identity=26.9911504424779, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI4505771, Length=239, Percent_Identity=26.7782426778243, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI9961250, Length=239, Percent_Identity=26.7782426778243, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI9961252, Length=239, Percent_Identity=26.7782426778243, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI21536376, Length=202, Percent_Identity=22.7722772277228, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI105990541, Length=202, Percent_Identity=22.7722772277228, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI116734710, Length=176, Percent_Identity=29.5454545454545, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI150417984, Length=198, Percent_Identity=26.7676767676768, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI9955963, Length=236, Percent_Identity=26.6949152542373, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI42741659, Length=239, Percent_Identity=26.7782426778243, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI156105685, Length=234, Percent_Identity=26.4957264957265, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI9665248, Length=245, Percent_Identity=27.3469387755102, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI87082268, Length=235, Percent_Identity=45.531914893617, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI1789593, Length=232, Percent_Identity=31.4655172413793, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1789864, Length=251, Percent_Identity=27.0916334661355, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87081791, Length=238, Percent_Identity=30.2521008403361, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787089, Length=231, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786398, Length=215, Percent_Identity=31.1627906976744, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787712, Length=237, Percent_Identity=29.535864978903, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1787029, Length=231, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1786872, Length=229, Percent_Identity=31.0043668122271, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1789672, Length=225, Percent_Identity=29.3333333333333, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788450, Length=224, Percent_Identity=28.125, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081709, Length=230, Percent_Identity=29.5652173913043, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1790190, Length=213, Percent_Identity=28.6384976525822, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI87082267, Length=212, Percent_Identity=29.7169811320755, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789873, Length=218, Percent_Identity=27.9816513761468, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788761, Length=233, Percent_Identity=29.6137339055794, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790467, Length=217, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1788210, Length=208, Percent_Identity=32.6923076923077, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787792, Length=219, Percent_Identity=27.8538812785388, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI48995001, Length=224, Percent_Identity=27.2321428571429, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI48994943, Length=199, Percent_Identity=32.1608040201005, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788472, Length=220, Percent_Identity=24.0909090909091, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1789991, Length=210, Percent_Identity=26.6666666666667, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787370, Length=218, Percent_Identity=27.5229357798165, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1786319, Length=225, Percent_Identity=30.2222222222222, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787164, Length=216, Percent_Identity=32.4074074074074, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1786253, Length=240, Percent_Identity=29.5833333333333, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788897, Length=211, Percent_Identity=25.5924170616114, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790162, Length=246, Percent_Identity=25.2032520325203, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786563, Length=221, Percent_Identity=31.6742081447964, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790525, Length=228, Percent_Identity=21.9298245614035, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1788165, Length=218, Percent_Identity=27.5229357798165, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788225, Length=223, Percent_Identity=29.5964125560538, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87081834, Length=197, Percent_Identity=26.9035532994924, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081782, Length=219, Percent_Identity=27.8538812785388, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786698, Length=203, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1787105, Length=221, Percent_Identity=26.6968325791855, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787500, Length=221, Percent_Identity=24.8868778280543, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1788506, Length=209, Percent_Identity=24.8803827751196, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1788053, Length=164, Percent_Identity=33.5365853658537, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1789032, Length=235, Percent_Identity=25.531914893617, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI48994997, Length=236, Percent_Identity=24.1525423728814, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789963, Length=235, Percent_Identity=24.6808510638298, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786975, Length=217, Percent_Identity=26.2672811059908, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1786654, Length=233, Percent_Identity=25.7510729613734, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787112, Length=239, Percent_Identity=27.1966527196653, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789586, Length=226, Percent_Identity=26.1061946902655, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786703, Length=204, Percent_Identity=26.9607843137255, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1788002, Length=219, Percent_Identity=28.310502283105, Blast_Score=60, Evalue=9e-11,
Organism=Escherichia coli, GI1787758, Length=210, Percent_Identity=27.6190476190476, Blast_Score=60, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17510237, Length=231, Percent_Identity=25.5411255411255, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17565586, Length=202, Percent_Identity=30.6930693069307, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71983523, Length=219, Percent_Identity=28.310502283105, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71983510, Length=219, Percent_Identity=28.310502283105, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71983516, Length=219, Percent_Identity=28.310502283105, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115533608, Length=216, Percent_Identity=25.9259259259259, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI212646699, Length=225, Percent_Identity=28, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71984940, Length=219, Percent_Identity=27.3972602739726, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193209708, Length=241, Percent_Identity=26.9709543568465, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17541710, Length=222, Percent_Identity=27.4774774774775, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17569145, Length=216, Percent_Identity=25.462962962963, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17569143, Length=216, Percent_Identity=26.3888888888889, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17558664, Length=239, Percent_Identity=25.1046025104602, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI115533592, Length=233, Percent_Identity=24.8927038626609, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25146777, Length=221, Percent_Identity=24.8868778280543, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24643674, Length=210, Percent_Identity=28.0952380952381, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221512771, Length=220, Percent_Identity=26.3636363636364, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221500365, Length=223, Percent_Identity=28.2511210762332, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI116007184, Length=223, Percent_Identity=28.2511210762332, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24666092, Length=212, Percent_Identity=24.5283018867925, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24650855, Length=210, Percent_Identity=24.2857142857143, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24650853, Length=209, Percent_Identity=24.4019138755981, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28573573, Length=198, Percent_Identity=28.7878787878788, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28573571, Length=198, Percent_Identity=28.7878787878788, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281362751, Length=212, Percent_Identity=25, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648314, Length=217, Percent_Identity=22.5806451612903, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24641565, Length=234, Percent_Identity=24.7863247863248, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI116007328, Length=208, Percent_Identity=26.9230769230769, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI28574744, Length=203, Percent_Identity=26.1083743842365, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574533, Length=223, Percent_Identity=23.3183856502242, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28574150, Length=208, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281363262, Length=217, Percent_Identity=24.8847926267281, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24653245, Length=217, Percent_Identity=24.8847926267281, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI19920532, Length=216, Percent_Identity=25.9259259259259, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24580930, Length=216, Percent_Identity=25.9259259259259, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 25485; Mature: 25485

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQLDRVSLSYGSFRALDNISLHAGAGELVVLLGANGAGKSSIFLAMSGIHRISGGSMRF
CCEECCEEECCCCEEECCCCEEECCCCCEEEEEECCCCCCCEEEEEECCHHCCCCCCEEE
DGRELSGMKPSQIVQAGLVHCPEGRKLFPAMSVEKNLTLGAYVHRRDRAGIRKTLEEVYE
CCCCCCCCCHHHHHHHHCEECCCCCEECCCCCCCCCCEEHHEEHHHHHHHHHHHHHHHHH
MFPILFQKKDDPAGSLSGGQQQMVALGRALMSRPRALLLDEPSLGLAPLVVKQMFEIIQR
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHH
INRAGTTVLLAEQNAYAALGIAHRAYVIENGHIVMEGDRDTLLKDEGIRKAYIGG
HHCCCCEEEEECCCCEEEEEEEEEEEEEECCEEEEECCCCCCCCCCCCCEECCCC
>Mature Secondary Structure
MLQLDRVSLSYGSFRALDNISLHAGAGELVVLLGANGAGKSSIFLAMSGIHRISGGSMRF
CCEECCEEECCCCEEECCCCEEECCCCCEEEEEECCCCCCCEEEEEECCHHCCCCCCEEE
DGRELSGMKPSQIVQAGLVHCPEGRKLFPAMSVEKNLTLGAYVHRRDRAGIRKTLEEVYE
CCCCCCCCCHHHHHHHHCEECCCCCEECCCCCCCCCCEEHHEEHHHHHHHHHHHHHHHHH
MFPILFQKKDDPAGSLSGGQQQMVALGRALMSRPRALLLDEPSLGLAPLVVKQMFEIIQR
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHH
INRAGTTVLLAEQNAYAALGIAHRAYVIENGHIVMEGDRDTLLKDEGIRKAYIGG
HHCCCCEEEEECCCCEEEEEEEEEEEEEECCEEEEECCCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]