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The map label for this gene is livJ [C]

Identifier: 94312904

GI number: 94312904

Start: 542665

End: 543837

Strand: Reverse

Name: livJ [C]

Synonym: Rmet_3976

Alternate gene names: 94312904

Gene position: 543837-542665 (Counterclockwise)

Preceding gene: 94312905

Following gene: 291481503

Centisome position: 21.08

GC content: 63.34

Gene sequence:

>1173_bases
ATGCAGTACAAGACATTGGGTCGTTTCTTCCCGCTGGCCGCGTGCGCGGTGGCGGCATTGGTCGCATCGGCACCGACACT
GGCGCAGGAAGTGGTCAAGATCGGCTACACGGGCCCGCTGTCGGGTGGCGCGGCGCTCTACGGCAAGAACGTGCTGTCGG
GCATCCAGATGGCTGTCGACGAGATCAACGCCAGCGGCCTCGAGGTCAAGGGTAAGAAGATCAAGCTGGAAGTCGTCTCG
CTCGACGACAAGTACGCGCCTGCCGAAGCCGCGATCAATGCGCGTCGCCTGGTGCAGCAGTACAAGACCCCGGCGGTGTT
CGTACCCCACTCGGGCGGCATCTTCGCGCTGCAGGCCTTCAATGAGCAGGAAAAGTTCATGGTGATGGCGTACTCGAGCG
TGCCGCGCATTACCGACGCCGGCAACAAGCTGACGATCCGCATCCCACCGGCATACACCGGCTACATCGAGCCGTTCGTC
AAGGCGCAGATGAAGCGCTTTGGCAAGAACGTGGCGCTGGTACCTGCCGACCATGACTACGCCAAGGCCTGGGTGCAAGC
GTTCGTGCCCGCCTGGGAAGGCGCGGGCGGCAAGGTGGTGGGCAACAATCCGATGTCGTACACCAAGGCCACCGACTTCT
ACACTGGCGTTTCGCGCGCCATCGCCGACAAACCCGACGTGATGTTCGTCGGCGGCCCGTCGGAGCCGACCGCGCTGGTG
GTCAAGCAGGCACGCGAGCTTGGCTTCAAGGGCGGCTTCATCATCATGGACCAGGCCAAGATGGACGAAATGGCCAAGGT
CACGAACGGTCTGGCCATGCTCGAAGGCTCGATCGGCGTGATGCCGCTGGTCAACGATGCACGCCCGGTGGCACAGGCCT
ATAACGCCCGCTACAAGAAGCTGCACGACGGTCGCGATGCCACCACGGAAATGTCGCTGAACTACACGATGACCTGGGCG
CTGGCCAATGCCATGAAGCTGGCCGGCACCACCAGCGACGCAGCGGCCATCCGCGCGAAAATGCCCGAGGCGGTCAAGAC
GCTGCCGAAGGATGTCAATCCGAACGAGGTGGAAGGCATCGACGCCAAGGGCGGCTCCATGGCCGATACCGTGGTGGGCT
GGGTGCAGAACGGCAAGATCGCGCCGGTACGCCTGTCGGAGTTGGGCAAGTAA

Upstream 100 bases:

>100_bases
CGATTCGCCGAACCGAGTAGATACACCCTTCTCTCACTCGCGGGAAAAGGGCGATAAGGGCGTAGAGAACAAGCGCAGTC
AATACTCAAAGGAGACAGCA

Downstream 100 bases:

>100_bases
GCAGTCCGCCGGAGAGAGACGACGGAGGAGTGGGACCCTGCTGGCGGGCAAGCCAGCGCCATAACTAGAATCGCAAGTGG
AGAGAAAGCAAAGGGCGTCC

Product: ABC-type branched-chain amino acid transport systems, livK-like periplasmic component

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched Chain Amino Acid ABC Transporter; Branched-Chain Amino Acid Transporter; Amino Acid ABC Transporter Periplasmic Protein; ABC-Type Branched-Chain Amino Acid Transport Systems; Leucine Transporter Subunit; Branched-Chain Amino Acid ABC Transporter

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MQYKTLGRFFPLAACAVAALVASAPTLAQEVVKIGYTGPLSGGAALYGKNVLSGIQMAVDEINASGLEVKGKKIKLEVVS
LDDKYAPAEAAINARRLVQQYKTPAVFVPHSGGIFALQAFNEQEKFMVMAYSSVPRITDAGNKLTIRIPPAYTGYIEPFV
KAQMKRFGKNVALVPADHDYAKAWVQAFVPAWEGAGGKVVGNNPMSYTKATDFYTGVSRAIADKPDVMFVGGPSEPTALV
VKQARELGFKGGFIIMDQAKMDEMAKVTNGLAMLEGSIGVMPLVNDARPVAQAYNARYKKLHDGRDATTEMSLNYTMTWA
LANAMKLAGTTSDAAAIRAKMPEAVKTLPKDVNPNEVEGIDAKGGSMADTVVGWVQNGKIAPVRLSELGK

Sequences:

>Translated_390_residues
MQYKTLGRFFPLAACAVAALVASAPTLAQEVVKIGYTGPLSGGAALYGKNVLSGIQMAVDEINASGLEVKGKKIKLEVVS
LDDKYAPAEAAINARRLVQQYKTPAVFVPHSGGIFALQAFNEQEKFMVMAYSSVPRITDAGNKLTIRIPPAYTGYIEPFV
KAQMKRFGKNVALVPADHDYAKAWVQAFVPAWEGAGGKVVGNNPMSYTKATDFYTGVSRAIADKPDVMFVGGPSEPTALV
VKQARELGFKGGFIIMDQAKMDEMAKVTNGLAMLEGSIGVMPLVNDARPVAQAYNARYKKLHDGRDATTEMSLNYTMTWA
LANAMKLAGTTSDAAAIRAKMPEAVKTLPKDVNPNEVEGIDAKGGSMADTVVGWVQNGKIAPVRLSELGK
>Mature_390_residues
MQYKTLGRFFPLAACAVAALVASAPTLAQEVVKIGYTGPLSGGAALYGKNVLSGIQMAVDEINASGLEVKGKKIKLEVVS
LDDKYAPAEAAINARRLVQQYKTPAVFVPHSGGIFALQAFNEQEKFMVMAYSSVPRITDAGNKLTIRIPPAYTGYIEPFV
KAQMKRFGKNVALVPADHDYAKAWVQAFVPAWEGAGGKVVGNNPMSYTKATDFYTGVSRAIADKPDVMFVGGPSEPTALV
VKQARELGFKGGFIIMDQAKMDEMAKVTNGLAMLEGSIGVMPLVNDARPVAQAYNARYKKLHDGRDATTEMSLNYTMTWA
LANAMKLAGTTSDAAAIRAKMPEAVKTLPKDVNPNEVEGIDAKGGSMADTVVGWVQNGKIAPVRLSELGK

Specific function: This Protein Is A Component Of The Leucine, Isoleucine, Valine, (Threonine) Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids. [C]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994941, Length=251, Percent_Identity=27.0916334661355, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 41660; Mature: 41660

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYKTLGRFFPLAACAVAALVASAPTLAQEVVKIGYTGPLSGGAALYGKNVLSGIQMAVD
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
EINASGLEVKGKKIKLEVVSLDDKYAPAEAAINARRLVQQYKTPAVFVPHSGGIFALQAF
HCCCCCEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEC
NEQEKFMVMAYSSVPRITDAGNKLTIRIPPAYTGYIEPFVKAQMKRFGKNVALVPADHDY
CCCCCEEEEEECCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCHH
AKAWVQAFVPAWEGAGGKVVGNNPMSYTKATDFYTGVSRAIADKPDVMFVGGPSEPTALV
HHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHH
VKQARELGFKGGFIIMDQAKMDEMAKVTNGLAMLEGSIGVMPLVNDARPVAQAYNARYKK
HHHHHHCCCCCCEEEEECHHHHHHHHHHCCCEEECCCCCEEEECCCCHHHHHHHHHHHHH
LHDGRDATTEMSLNYTMTWALANAMKLAGTTSDAAAIRAKMPEAVKTLPKDVNPNEVEGI
HCCCCCCCEEEEEEEEEEEHHHHHHHHCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCCC
DAKGGSMADTVVGWVQNGKIAPVRLSELGK
CCCCCCHHHHHHHHHCCCCCCEEEHHCCCC
>Mature Secondary Structure
MQYKTLGRFFPLAACAVAALVASAPTLAQEVVKIGYTGPLSGGAALYGKNVLSGIQMAVD
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
EINASGLEVKGKKIKLEVVSLDDKYAPAEAAINARRLVQQYKTPAVFVPHSGGIFALQAF
HCCCCCEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEC
NEQEKFMVMAYSSVPRITDAGNKLTIRIPPAYTGYIEPFVKAQMKRFGKNVALVPADHDY
CCCCCEEEEEECCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCHH
AKAWVQAFVPAWEGAGGKVVGNNPMSYTKATDFYTGVSRAIADKPDVMFVGGPSEPTALV
HHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHH
VKQARELGFKGGFIIMDQAKMDEMAKVTNGLAMLEGSIGVMPLVNDARPVAQAYNARYKK
HHHHHHCCCCCCEEEEECHHHHHHHHHHCCCEEECCCCCEEEECCCCHHHHHHHHHHHHH
LHDGRDATTEMSLNYTMTWALANAMKLAGTTSDAAAIRAKMPEAVKTLPKDVNPNEVEGI
HCCCCCCCEEEEEEEEEEEHHHHHHHHCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCCC
DAKGGSMADTVVGWVQNGKIAPVRLSELGK
CCCCCCHHHHHHHHHCCCCCCEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA