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The map label for this gene is rhtB [H]

Identifier: 94312839

GI number: 94312839

Start: 459280

End: 459906

Strand: Direct

Name: rhtB [H]

Synonym: Rmet_3911

Alternate gene names: 94312839

Gene position: 459280-459906 (Clockwise)

Preceding gene: 94312838

Following gene: 94312843

Centisome position: 17.8

GC content: 64.11

Gene sequence:

>627_bases
ATGCCGCCCTTCGATACGCTGCTGCCGTTTCTCGGCATCGCAGTCCTGCTCAGCCTCGCGCCGGGACCTGACAATATTTT
CGTGTTGTTGCTGTCAGCGATGCATGGCACGCGCGCCGGCCTGACCGTGGTGTTGGGACTTTGCACGGGACTGCTGGTTC
ACACGGCGGTGGTGGCCGTGGGCCTGGCTGCGTTGCTGGCGGCATCGACGGTGGCGTTCACCGTGTTGAAGATCGCTGGC
GCGCTGTATCTCGTCTATCTGGCGTGGCAGGCGTTCCGCGCTCCCGTGGGCGAGCTGTCGTCTTCCCAGATGCAGGTGAA
GACCGGCAAGCACATGTACCTGCGCGGGGTGATCATGAACCTGACCAACCCCAAGGTCGTGATCTTCTTCCTGGCATTCC
TGCCGCAGTTCGTCAGCCCGGATCGGGGCCCGGTGGCCCCTCAGATCATGGTGCTTGGCTTCGTGTTCATCCTTGCCACG
CTGCTGGTATTCGGCTCGATCGCCTGGTTTTCGGGCGCGTTCGGCCGCGTGCTGATGCGCTCCGCGCGGATGCAACGCGC
GGTGAACTGGTTCGCCGGAAGTGTGTTCCTGGCGCTCGCCGGCAGGCTGGCGCTGTCTCAGCGGTAA

Upstream 100 bases:

>100_bases
CCTGCGCCTGAAGACCAAGGCCGAGGCGATCCGCGCGGCGGCAATTGCAGAGCTGGTCTGAACATCGTTACCATGACCGC
TCCATCCTTCGGAGCCGTTC

Downstream 100 bases:

>100_bases
GGGATCGGCCCCGTGCGGGGCCGGTGTTCACGCGAAGGTGATCGCGGGACGCCGCTCCGGCAGTTCCAGGTGCGGCACGT
TGTTGAAGCTCAGCAGGCGC

Product: putative amino acid (threonine) efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MPPFDTLLPFLGIAVLLSLAPGPDNIFVLLLSAMHGTRAGLTVVLGLCTGLLVHTAVVAVGLAALLAASTVAFTVLKIAG
ALYLVYLAWQAFRAPVGELSSSQMQVKTGKHMYLRGVIMNLTNPKVVIFFLAFLPQFVSPDRGPVAPQIMVLGFVFILAT
LLVFGSIAWFSGAFGRVLMRSARMQRAVNWFAGSVFLALAGRLALSQR

Sequences:

>Translated_208_residues
MPPFDTLLPFLGIAVLLSLAPGPDNIFVLLLSAMHGTRAGLTVVLGLCTGLLVHTAVVAVGLAALLAASTVAFTVLKIAG
ALYLVYLAWQAFRAPVGELSSSQMQVKTGKHMYLRGVIMNLTNPKVVIFFLAFLPQFVSPDRGPVAPQIMVLGFVFILAT
LLVFGSIAWFSGAFGRVLMRSARMQRAVNWFAGSVFLALAGRLALSQR
>Mature_207_residues
PPFDTLLPFLGIAVLLSLAPGPDNIFVLLLSAMHGTRAGLTVVLGLCTGLLVHTAVVAVGLAALLAASTVAFTVLKIAGA
LYLVYLAWQAFRAPVGELSSSQMQVKTGKHMYLRGVIMNLTNPKVVIFFLAFLPQFVSPDRGPVAPQIMVLGFVFILATL
LVFGSIAWFSGAFGRVLMRSARMQRAVNWFAGSVFLALAGRLALSQR

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=199, Percent_Identity=35.678391959799, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1788099, Length=201, Percent_Identity=29.3532338308458, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI48994978, Length=198, Percent_Identity=24.7474747474747, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1786522, Length=210, Percent_Identity=27.1428571428571, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22203; Mature: 22072

Theoretical pI: Translated: 11.74; Mature: 11.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPFDTLLPFLGIAVLLSLAPGPDNIFVLLLSAMHGTRAGLTVVLGLCTGLLVHTAVVAV
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GLAALLAASTVAFTVLKIAGALYLVYLAWQAFRAPVGELSSSQMQVKTGKHMYLRGVIMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHCCCHHHHHHHHHH
LTNPKVVIFFLAFLPQFVSPDRGPVAPQIMVLGFVFILATLLVFGSIAWFSGAFGRVLMR
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SARMQRAVNWFAGSVFLALAGRLALSQR
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PPFDTLLPFLGIAVLLSLAPGPDNIFVLLLSAMHGTRAGLTVVLGLCTGLLVHTAVVAV
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GLAALLAASTVAFTVLKIAGALYLVYLAWQAFRAPVGELSSSQMQVKTGKHMYLRGVIMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHCCCHHHHHHHHHH
LTNPKVVIFFLAFLPQFVSPDRGPVAPQIMVLGFVFILATLLVFGSIAWFSGAFGRVLMR
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SARMQRAVNWFAGSVFLALAGRLALSQR
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]