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The map label for this gene is def1 [H]

Identifier: 94312496

GI number: 94312496

Start: 3867045

End: 3867551

Strand: Reverse

Name: def1 [H]

Synonym: Rmet_3565

Alternate gene names: 94312496

Gene position: 3867551-3867045 (Counterclockwise)

Preceding gene: 94312501

Following gene: 94312495

Centisome position: 98.46

GC content: 60.75

Gene sequence:

>507_bases
ATGGCAAAACTCGACATCCTCACCTACCCCGATCCTCGCCTGCACAAAGTGGCCAAGCCCGTGGAGGTGGTGGACGACCG
CATCCGCCAGCTCGTGAAAGACATGGCCGAAACCATGTACGAAGCGCCCGGCATCGGCCTGGCCGCGACGCAAGTGGACG
TGCACGAGCGCGTGATCGTGATCGACATTTCCGAGAGCCGCGACGAGTTGATGGTGTTCATCAACCCCGAGGTCACTTGG
GCCAGCGAGAACCGCAAGGTATGGGAAGAGGGCTGCCTGTCGGTGCCCGAAGTCTATGACCGCGTGGAGCGCCCGGATCG
CGTCAAGGTACGCGCGCTCGACGAGAAGGGCGAAGCGTTCGAGCTCGACGCCGACGATCTGCTCGCTGTATGTATACAGC
ACGAGATCGATCACCTCGACGGCAAGGTATTCGTGGAATATCTGTCGCCACTCAAGCTCCAACGCATCAAGAGCAAGCTC
CAGAAGCGCGTGCGCTCGCGCATGTAA

Upstream 100 bases:

>100_bases
CCTTGAATTTTTGTACTAGCGCCCCGATTCTGCATGGAAACCCCGGTCGCCGCAACGGCGGCTGGACCGTGGACAGCACC
CGGCGTTGCGCCCGAGCATC

Downstream 100 bases:

>100_bases
GGATGTAACAATCGCGCGGAAGCGGAAACGCCTCTGCTAAGGTTACAGCTTCCGAAGAACACATACGTACCCGGCCGCTA
TGCGGCCGGTGTCGTTTTCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MAKLDILTYPDPRLHKVAKPVEVVDDRIRQLVKDMAETMYEAPGIGLAATQVDVHERVIVIDISESRDELMVFINPEVTW
ASENRKVWEEGCLSVPEVYDRVERPDRVKVRALDEKGEAFELDADDLLAVCIQHEIDHLDGKVFVEYLSPLKLQRIKSKL
QKRVRSRM

Sequences:

>Translated_168_residues
MAKLDILTYPDPRLHKVAKPVEVVDDRIRQLVKDMAETMYEAPGIGLAATQVDVHERVIVIDISESRDELMVFINPEVTW
ASENRKVWEEGCLSVPEVYDRVERPDRVKVRALDEKGEAFELDADDLLAVCIQHEIDHLDGKVFVEYLSPLKLQRIKSKL
QKRVRSRM
>Mature_167_residues
AKLDILTYPDPRLHKVAKPVEVVDDRIRQLVKDMAETMYEAPGIGLAATQVDVHERVIVIDISESRDELMVFINPEVTWA
SENRKVWEEGCLSVPEVYDRVERPDRVKVRALDEKGEAFELDADDLLAVCIQHEIDHLDGKVFVEYLSPLKLQRIKSKLQ
KRVRSRM

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=169, Percent_Identity=58.5798816568047, Blast_Score=193, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24645728, Length=157, Percent_Identity=31.8471337579618, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24645726, Length=157, Percent_Identity=29.2993630573248, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19408; Mature: 19276

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLDILTYPDPRLHKVAKPVEVVDDRIRQLVKDMAETMYEAPGIGLAATQVDVHERVIV
CCCEEEEECCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEE
IDISESRDELMVFINPEVTWASENRKVWEEGCLSVPEVYDRVERPDRVKVRALDEKGEAF
EEECCCCCEEEEEECCCEEECCCCCHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCEE
ELDADDLLAVCIQHEIDHLDGKVFVEYLSPLKLQRIKSKLQKRVRSRM
EECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKLDILTYPDPRLHKVAKPVEVVDDRIRQLVKDMAETMYEAPGIGLAATQVDVHERVIV
CCEEEEECCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEE
IDISESRDELMVFINPEVTWASENRKVWEEGCLSVPEVYDRVERPDRVKVRALDEKGEAF
EEECCCCCEEEEEECCCEEECCCCCHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCEE
ELDADDLLAVCIQHEIDHLDGKVFVEYLSPLKLQRIKSKLQKRVRSRM
EECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]