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The map label for this gene is fmt [H]

Identifier: 94312495

GI number: 94312495

Start: 3865931

End: 3866965

Strand: Reverse

Name: fmt [H]

Synonym: Rmet_3564

Alternate gene names: 94312495

Gene position: 3866965-3865931 (Counterclockwise)

Preceding gene: 94312496

Following gene: 94312494

Centisome position: 98.44

GC content: 69.66

Gene sequence:

>1035_bases
ATGCGGCCGGTGTCGTTTTCATTGCGGAATATCGTCATGACCCAAGCCCAGCCACTGCGCGTCGCTTTTGCGGGAACGCC
GGAATTCGCGCGCGTCGCCCTTGAGGCCATTCACGCTGCCGGATTTCCGGTGGTCGCCGTGCTGACCCAGCCAGACCGGC
CCGCCGGCCGCGGCATGCAATTGCAGGCCAGCCCGGTCAAACAGTTTGCCGTCGAGGCCGGCCTGGGCCCAGTGCTGCAG
CCACGCTCGTTGCGCCGCCAGGGCAAGTATCCGGAGGAAGCCGGCGCGGCCGTCGACACGCTCGCGGAAATCGCACCCGA
TGTGATGGTGGTGGCCGCCTACGGGCTGATTCTGCCGACCGAAGTCCTTGAGTTGCCGCGTCATGGCTGCCTGAATATCC
ATGCCTCGCTGCTGCCGCGGTGGCGCGGCGCCGCGCCGATCCACCGCGCGATCGAGGCGGGGGACCCGGAGACCGGCATC
ACGCTGATGCAGATGGACGAGGGGCTCGACACCGGCGCGATGCTGTCGCGCGAGTCCACGCCGATCGGCCCCCAGGACAG
TACCGGCACGCTGCATGACACACTGGCCGCGCTCGGCGGGCGCATGATCGTGGACGCGCTGCGCCAGCTCGCCGCAGGCC
AGCCGCTCACCGCCACGCCGCAGCCCGAAGCCGGCGTCACCTACGCGGAAAAGATCGCTAAGGATGAGGCACCGCTCGAC
CTGACGCGCCCGGCCATCGAACTGGCGAACCAGATCCGCGCCTTCAATCCGTTCCCGGGCGCCACGGTGCAAGTCGGCGA
CACCATCATCAAGTGCTGGCAGGCGCTGCCGCTGGCTGCCGCCACGAGCCTGCCGCATCCCCCGGGTACGATCCTGGCCG
CCGACACAGCCGGCGTGATCATCGCCTGCGGCAATGGCAGCGCCCTGCAGGTCACCGAGCTGCAGAAGCCCGGCGGCCGG
CGCCAGCCGGCGCAACAGTTCCTGCAGGCCATGCCGCTGCCACCGGGAACGCGCTGCCTGATTGCCGGCGCCTAG

Upstream 100 bases:

>100_bases
CGCGTGCGCTCGCGCATGTAAGGATGTAACAATCGCGCGGAAGCGGAAACGCCTCTGCTAAGGTTACAGCTTCCGAAGAA
CACATACGTACCCGGCCGCT

Downstream 100 bases:

>100_bases
CGGGCACTGCGTGGCGAGTGCTGCAGCAATGTGACGCCGGATTATCACAATGCGTAAAACACCGCCTCCGGCATTGAATT
TCCACGGTTCGCACCCTATC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MRPVSFSLRNIVMTQAQPLRVAFAGTPEFARVALEAIHAAGFPVVAVLTQPDRPAGRGMQLQASPVKQFAVEAGLGPVLQ
PRSLRRQGKYPEEAGAAVDTLAEIAPDVMVVAAYGLILPTEVLELPRHGCLNIHASLLPRWRGAAPIHRAIEAGDPETGI
TLMQMDEGLDTGAMLSRESTPIGPQDSTGTLHDTLAALGGRMIVDALRQLAAGQPLTATPQPEAGVTYAEKIAKDEAPLD
LTRPAIELANQIRAFNPFPGATVQVGDTIIKCWQALPLAAATSLPHPPGTILAADTAGVIIACGNGSALQVTELQKPGGR
RQPAQQFLQAMPLPPGTRCLIAGA

Sequences:

>Translated_344_residues
MRPVSFSLRNIVMTQAQPLRVAFAGTPEFARVALEAIHAAGFPVVAVLTQPDRPAGRGMQLQASPVKQFAVEAGLGPVLQ
PRSLRRQGKYPEEAGAAVDTLAEIAPDVMVVAAYGLILPTEVLELPRHGCLNIHASLLPRWRGAAPIHRAIEAGDPETGI
TLMQMDEGLDTGAMLSRESTPIGPQDSTGTLHDTLAALGGRMIVDALRQLAAGQPLTATPQPEAGVTYAEKIAKDEAPLD
LTRPAIELANQIRAFNPFPGATVQVGDTIIKCWQALPLAAATSLPHPPGTILAADTAGVIIACGNGSALQVTELQKPGGR
RQPAQQFLQAMPLPPGTRCLIAGA
>Mature_344_residues
MRPVSFSLRNIVMTQAQPLRVAFAGTPEFARVALEAIHAAGFPVVAVLTQPDRPAGRGMQLQASPVKQFAVEAGLGPVLQ
PRSLRRQGKYPEEAGAAVDTLAEIAPDVMVVAAYGLILPTEVLELPRHGCLNIHASLLPRWRGAAPIHRAIEAGDPETGI
TLMQMDEGLDTGAMLSRESTPIGPQDSTGTLHDTLAALGGRMIVDALRQLAAGQPLTATPQPEAGVTYAEKIAKDEAPLD
LTRPAIELANQIRAFNPFPGATVQVGDTIIKCWQALPLAAATSLPHPPGTILAADTAGVIIACGNGSALQVTELQKPGGR
RQPAQQFLQAMPLPPGTRCLIAGA

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI238814322, Length=322, Percent_Identity=27.9503105590062, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI21614513, Length=321, Percent_Identity=28.0373831775701, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI164663775, Length=272, Percent_Identity=32.7205882352941, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1789683, Length=329, Percent_Identity=49.8480243161094, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1788589, Length=314, Percent_Identity=29.2993630573248, Blast_Score=125, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI133930964, Length=327, Percent_Identity=24.7706422018349, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319458, Length=368, Percent_Identity=26.0869565217391, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550868, Length=317, Percent_Identity=26.813880126183, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI28571984, Length=224, Percent_Identity=31.6964285714286, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585660, Length=253, Percent_Identity=25.6916996047431, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 36056; Mature: 36056

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPVSFSLRNIVMTQAQPLRVAFAGTPEFARVALEAIHAAGFPVVAVLTQPDRPAGRGMQ
CCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCE
LQASPVKQFAVEAGLGPVLQPRSLRRQGKYPEEAGAAVDTLAEIAPDVMVVAAYGLILPT
EECCHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EVLELPRHGCLNIHASLLPRWRGAAPIHRAIEAGDPETGITLMQMDEGLDTGAMLSREST
HHHHCCCCCCEEHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCHHHCCCCC
PIGPQDSTGTLHDTLAALGGRMIVDALRQLAAGQPLTATPQPEAGVTYAEKIAKDEAPLD
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCC
LTRPAIELANQIRAFNPFPGATVQVGDTIIKCWQALPLAAATSLPHPPGTILAADTAGVI
HHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCHHHHHCCCCCCCCEEEECCCCEE
IACGNGSALQVTELQKPGGRRQPAQQFLQAMPLPPGTRCLIAGA
EEECCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEECC
>Mature Secondary Structure
MRPVSFSLRNIVMTQAQPLRVAFAGTPEFARVALEAIHAAGFPVVAVLTQPDRPAGRGMQ
CCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCE
LQASPVKQFAVEAGLGPVLQPRSLRRQGKYPEEAGAAVDTLAEIAPDVMVVAAYGLILPT
EECCHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EVLELPRHGCLNIHASLLPRWRGAAPIHRAIEAGDPETGITLMQMDEGLDTGAMLSREST
HHHHCCCCCCEEHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCHHHCCCCC
PIGPQDSTGTLHDTLAALGGRMIVDALRQLAAGQPLTATPQPEAGVTYAEKIAKDEAPLD
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCC
LTRPAIELANQIRAFNPFPGATVQVGDTIIKCWQALPLAAATSLPHPPGTILAADTAGVI
HHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCHHHHHCCCCCCCCEEEECCCCEE
IACGNGSALQVTELQKPGGRRQPAQQFLQAMPLPPGTRCLIAGA
EEECCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA