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The map label for this gene is priA [H]

Identifier: 94312421

GI number: 94312421

Start: 3778718

End: 3781012

Strand: Reverse

Name: priA [H]

Synonym: Rmet_3490

Alternate gene names: 94312421

Gene position: 3781012-3778718 (Counterclockwise)

Preceding gene: 335055671

Following gene: 94312420

Centisome position: 96.26

GC content: 66.54

Gene sequence:

>2295_bases
ATGACATCGGCGGCGGCGCCTGTGGATACTGCGTTACAGCCCGCATCCGCTGAGGCTGCGGCGGGCCTCGTGCGCGTCGC
ATTGGATACCCCCGCTGACGACTACTTCGACTATCTGGCGGATGCCGCGGTGATGCCGGGCGAGCTCGTGGTCGTGCCAT
TTGGCAAGCGCCATGTCATGGGGGTCGTGCTCGATCGACCCGACAACACCGAGGTACCCGCAGACCGCCTGAGGCCGGTG
ACGGGCCATCTGACGTGGATGCCACCGCTCAACGCCGCGTGGATCGGCCTTGCCCGTTTTGCCGCGCGCTACTACCACCG
CTGTCTGGGCGAAGTGATGCTGCCCGCGCTGCCGCCGTCGCTGCGCGATCCGCAGACGTGGGAACGGCTGGAACAACGCG
CCCGAACCACGCAGTACCGGGTACGCCCGGCGCAGTCAGAAGCCTTGCTGGCGGCAGCGCCGTCGCGCGCACGAACCGTG
CGGGCGCTGGCTGAAGCGTTGATTGCGGCGCCTGATGGGTTGCGCCTTGCAGAAGCCCGCGAGCTTTGCACGGCGGCCCC
GGCACGCCTGGCCGAGTGGGAGGCCGCTGGTTGTATCCAAAGCACCCATGCAGACCGTCCGCTGCTCGATAAAGCCCCCA
CGCAGGTACCATCTGTCGCCCCGACGCTCCATGACGAGCAGCGTGCGGCCGTGGATGCCATCACCGCCGCCGAAGGCTAT
GCCGCCTTCCTGCTCCACGGGGTGACCGGCAGCGGCAAGACGGAAGTCTATCTCCACGCCATCGCCGCGCGATTGGACGA
GGACCCAGCCGCCCAGGTATTGATGCTGGTCCCCGAGATCAACCTCACGCCCCAACTGCAGGCGCGTATCGCCGAGCGCT
TTCCTGACCAGCCACTGGCCGTCCTGCATAGTGGACTGGCCGATCAGGAGCGCAGCCTCCAGTGGCTGGCAGCGCATCGC
GGCGAGGCGCGTATCGTGCTCGGCACACGCATGGCCGTCATGGCATCCGTGCCGAACCTGAAACTGATCGTGGTCGATGA
AGAGCACGACACCTCCTACAAGCAGCAGGAAGGCCTGCGGTACTCGGCACGCGATCTGGCCGTGTGGCGAGCCAACCAGT
TAGGCATCCCGGTAGTGCTTGGCTCGGCCACACCGTCCATGGAGTCCTGGTACCGGGCGGAGCAGGGCGCCTATCGCAAG
CTGGTGCTGCGGGATCGCGCCCAGGCGGATGCCGCACTGCCCACGGTCAGGCTGATCGACCTGAACCATGAACGCCAGCG
TCAGCGCACGCTCTATGAAGGGCTGTCGGTGCCGCTGCTCGATGCCATCCAGCAGCGGCTGGATCGGGGTCAGCAGAGCC
TGTTGTTCCTGAACCGGCGGGGCTACGCGCCAGTCCTGGCCTGCGACAGTTGCGGCTGGCTCTCGGGCTGCCCGCGATGC
TCGGCGTACCTGGTGCTGCATCGCCCGGAGCGGCGGTTGCGCTGCCACCATTGCGGGCTTGAGGCGCCCGTGCCGCATCA
TTGCCCCGACTGCGGCAATGTCGACATCGCACCGCTTGGCCGGGGCACGCAGCGGATCGAGGAAGCGCTGGCCGCACGTT
TTCCACAGGCACGCATCGCCCGGATCGACGCGGATTCCACGCGGCGCAAGGGCAGTGCGCAGGCCATGCTCGACGAGGTC
CATGCCGGCGAGGTCGACATCCTGGTGGGCACGCAGATGGTGGCCAAGGGACACGACTTCCAGCGTGTCACGCTGGTGGG
CATCGTCCACCCTGATGCCGCCATGTTCAGTCACGATTTCCGCGCGGCCGAGCACCTGTTCGCGTCGCTGATGCAGGTGT
CTGGCCGCGCCGGCAGGGCAGGGCTGGGCGGCGAAGTGTTGATCCAGACCCGCTATCCAGATGCCCCCGCATTGCAGGCT
TTGGTTCGCCATGACTATGATGGATTCGCAGCCAGCCAGTTGCGAGAGCGCCGGCAGGCCGGGTTGCCCCCATATGCCTA
CCAGGTGCTGGTGACGGCCGAGCACGCGCAGCTCGAGCGCGCGCTTGCCTTCCTGCAGGCCGCTCGCGCGTGCGCGGATG
CCAGTCCGATGGCAGCCGAAGTGCAACTGCATGACCCGGTGCCGATGTCGATGGTGCGCCTGTTCAACAAAGAGCGCGCC
CAGATGCTCGTCGAAGCCGGCTCCCGTCCGTTATTGCAACGGTTTGTCAGTGAGTGGGTACGGACTTTCGATGATGCCGC
GACACGTGTCAAAGGGGTGCGTTGGCAATTGGAAATCGATCCGCTACGCATTTGA

Upstream 100 bases:

>100_bases
AGTTGATGTTGCGCCGCACACAGACCCGTTCGGAAAATCCTGTTACGCCAGCCATGCACAACCATCGCGCAGCCGGACTG
CACCACGCTGGAGCTGGTCA

Downstream 100 bases:

>100_bases
GCGGAGCAGTCGCCACGCCGGGAAGTTGCGAAAGTGCAACCTTTTGTCCGCAAGTGAGCTTAGGGGTTGCACTAGGTTGC
ACCCCGTAATTCTCCGAGAG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 764; Mature: 763

Protein sequence:

>764_residues
MTSAAAPVDTALQPASAEAAAGLVRVALDTPADDYFDYLADAAVMPGELVVVPFGKRHVMGVVLDRPDNTEVPADRLRPV
TGHLTWMPPLNAAWIGLARFAARYYHRCLGEVMLPALPPSLRDPQTWERLEQRARTTQYRVRPAQSEALLAAAPSRARTV
RALAEALIAAPDGLRLAEARELCTAAPARLAEWEAAGCIQSTHADRPLLDKAPTQVPSVAPTLHDEQRAAVDAITAAEGY
AAFLLHGVTGSGKTEVYLHAIAARLDEDPAAQVLMLVPEINLTPQLQARIAERFPDQPLAVLHSGLADQERSLQWLAAHR
GEARIVLGTRMAVMASVPNLKLIVVDEEHDTSYKQQEGLRYSARDLAVWRANQLGIPVVLGSATPSMESWYRAEQGAYRK
LVLRDRAQADAALPTVRLIDLNHERQRQRTLYEGLSVPLLDAIQQRLDRGQQSLLFLNRRGYAPVLACDSCGWLSGCPRC
SAYLVLHRPERRLRCHHCGLEAPVPHHCPDCGNVDIAPLGRGTQRIEEALAARFPQARIARIDADSTRRKGSAQAMLDEV
HAGEVDILVGTQMVAKGHDFQRVTLVGIVHPDAAMFSHDFRAAEHLFASLMQVSGRAGRAGLGGEVLIQTRYPDAPALQA
LVRHDYDGFAASQLRERRQAGLPPYAYQVLVTAEHAQLERALAFLQAARACADASPMAAEVQLHDPVPMSMVRLFNKERA
QMLVEAGSRPLLQRFVSEWVRTFDDAATRVKGVRWQLEIDPLRI

Sequences:

>Translated_764_residues
MTSAAAPVDTALQPASAEAAAGLVRVALDTPADDYFDYLADAAVMPGELVVVPFGKRHVMGVVLDRPDNTEVPADRLRPV
TGHLTWMPPLNAAWIGLARFAARYYHRCLGEVMLPALPPSLRDPQTWERLEQRARTTQYRVRPAQSEALLAAAPSRARTV
RALAEALIAAPDGLRLAEARELCTAAPARLAEWEAAGCIQSTHADRPLLDKAPTQVPSVAPTLHDEQRAAVDAITAAEGY
AAFLLHGVTGSGKTEVYLHAIAARLDEDPAAQVLMLVPEINLTPQLQARIAERFPDQPLAVLHSGLADQERSLQWLAAHR
GEARIVLGTRMAVMASVPNLKLIVVDEEHDTSYKQQEGLRYSARDLAVWRANQLGIPVVLGSATPSMESWYRAEQGAYRK
LVLRDRAQADAALPTVRLIDLNHERQRQRTLYEGLSVPLLDAIQQRLDRGQQSLLFLNRRGYAPVLACDSCGWLSGCPRC
SAYLVLHRPERRLRCHHCGLEAPVPHHCPDCGNVDIAPLGRGTQRIEEALAARFPQARIARIDADSTRRKGSAQAMLDEV
HAGEVDILVGTQMVAKGHDFQRVTLVGIVHPDAAMFSHDFRAAEHLFASLMQVSGRAGRAGLGGEVLIQTRYPDAPALQA
LVRHDYDGFAASQLRERRQAGLPPYAYQVLVTAEHAQLERALAFLQAARACADASPMAAEVQLHDPVPMSMVRLFNKERA
QMLVEAGSRPLLQRFVSEWVRTFDDAATRVKGVRWQLEIDPLRI
>Mature_763_residues
TSAAAPVDTALQPASAEAAAGLVRVALDTPADDYFDYLADAAVMPGELVVVPFGKRHVMGVVLDRPDNTEVPADRLRPVT
GHLTWMPPLNAAWIGLARFAARYYHRCLGEVMLPALPPSLRDPQTWERLEQRARTTQYRVRPAQSEALLAAAPSRARTVR
ALAEALIAAPDGLRLAEARELCTAAPARLAEWEAAGCIQSTHADRPLLDKAPTQVPSVAPTLHDEQRAAVDAITAAEGYA
AFLLHGVTGSGKTEVYLHAIAARLDEDPAAQVLMLVPEINLTPQLQARIAERFPDQPLAVLHSGLADQERSLQWLAAHRG
EARIVLGTRMAVMASVPNLKLIVVDEEHDTSYKQQEGLRYSARDLAVWRANQLGIPVVLGSATPSMESWYRAEQGAYRKL
VLRDRAQADAALPTVRLIDLNHERQRQRTLYEGLSVPLLDAIQQRLDRGQQSLLFLNRRGYAPVLACDSCGWLSGCPRCS
AYLVLHRPERRLRCHHCGLEAPVPHHCPDCGNVDIAPLGRGTQRIEEALAARFPQARIARIDADSTRRKGSAQAMLDEVH
AGEVDILVGTQMVAKGHDFQRVTLVGIVHPDAAMFSHDFRAAEHLFASLMQVSGRAGRAGLGGEVLIQTRYPDAPALQAL
VRHDYDGFAASQLRERRQAGLPPYAYQVLVTAEHAQLERALAFLQAARACADASPMAAEVQLHDPVPMSMVRLFNKERAQ
MLVEAGSRPLLQRFVSEWVRTFDDAATRVKGVRWQLEIDPLRI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=757, Percent_Identity=41.0832232496698, Blast_Score=488, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 83937; Mature: 83806

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSAAAPVDTALQPASAEAAAGLVRVALDTPADDYFDYLADAAVMPGELVVVPFGKRHVM
CCCCCCCCHHCCCCCCHHHHHCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCEEE
GVVLDRPDNTEVPADRLRPVTGHLTWMPPLNAAWIGLARFAARYYHRCLGEVMLPALPPS
EEEEECCCCCCCCHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LRDPQTWERLEQRARTTQYRVRPAQSEALLAAAPSRARTVRALAEALIAAPDGLRLAEAR
CCCCHHHHHHHHHHHHHHEEECCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHH
ELCTAAPARLAEWEAAGCIQSTHADRPLLDKAPTQVPSVAPTLHDEQRAAVDAITAAEGY
HHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
AAFLLHGVTGSGKTEVYLHAIAARLDEDPAAQVLMLVPEINLTPQLQARIAERFPDQPLA
EEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHH
VLHSGLADQERSLQWLAAHRGEARIVLGTRMAVMASVPNLKLIVVDEEHDTSYKQQEGLR
HHHCCCCCHHHHHHHHHHCCCCEEEEEECHHHHHCCCCCEEEEEEECCCCCCHHHHCCCC
YSARDLAVWRANQLGIPVVLGSATPSMESWYRAEQGAYRKLVLRDRAQADAALPTVRLID
CCCHHHEEEECCCCCCEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEE
LNHERQRQRTLYEGLSVPLLDAIQQRLDRGQQSLLFLNRRGYAPVLACDSCGWLSGCPRC
CCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHEEEEECCCCCCEEEECCCCCCCCCCCC
SAYLVLHRPERRLRCHHCGLEAPVPHHCPDCGNVDIAPLGRGTQRIEEALAARFPQARIA
EEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEE
RIDADSTRRKGSAQAMLDEVHAGEVDILVGTQMVAKGHDFQRVTLVGIVHPDAAMFSHDF
EECCCHHHCCCHHHHHHHHHCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHHHH
RAAEHLFASLMQVSGRAGRAGLGGEVLIQTRYPDAPALQALVRHDYDGFAASQLRERRQA
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHC
GLPPYAYQVLVTAEHAQLERALAFLQAARACADASPMAAEVQLHDPVPMSMVRLFNKERA
CCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
QMLVEAGSRPLLQRFVSEWVRTFDDAATRVKGVRWQLEIDPLRI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCC
>Mature Secondary Structure 
TSAAAPVDTALQPASAEAAAGLVRVALDTPADDYFDYLADAAVMPGELVVVPFGKRHVM
CCCCCCCHHCCCCCCHHHHHCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCEEE
GVVLDRPDNTEVPADRLRPVTGHLTWMPPLNAAWIGLARFAARYYHRCLGEVMLPALPPS
EEEEECCCCCCCCHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LRDPQTWERLEQRARTTQYRVRPAQSEALLAAAPSRARTVRALAEALIAAPDGLRLAEAR
CCCCHHHHHHHHHHHHHHEEECCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHH
ELCTAAPARLAEWEAAGCIQSTHADRPLLDKAPTQVPSVAPTLHDEQRAAVDAITAAEGY
HHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
AAFLLHGVTGSGKTEVYLHAIAARLDEDPAAQVLMLVPEINLTPQLQARIAERFPDQPLA
EEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHH
VLHSGLADQERSLQWLAAHRGEARIVLGTRMAVMASVPNLKLIVVDEEHDTSYKQQEGLR
HHHCCCCCHHHHHHHHHHCCCCEEEEEECHHHHHCCCCCEEEEEEECCCCCCHHHHCCCC
YSARDLAVWRANQLGIPVVLGSATPSMESWYRAEQGAYRKLVLRDRAQADAALPTVRLID
CCCHHHEEEECCCCCCEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEE
LNHERQRQRTLYEGLSVPLLDAIQQRLDRGQQSLLFLNRRGYAPVLACDSCGWLSGCPRC
CCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHEEEEECCCCCCEEEECCCCCCCCCCCC
SAYLVLHRPERRLRCHHCGLEAPVPHHCPDCGNVDIAPLGRGTQRIEEALAARFPQARIA
EEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEE
RIDADSTRRKGSAQAMLDEVHAGEVDILVGTQMVAKGHDFQRVTLVGIVHPDAAMFSHDF
EECCCHHHCCCHHHHHHHHHCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHHHH
RAAEHLFASLMQVSGRAGRAGLGGEVLIQTRYPDAPALQALVRHDYDGFAASQLRERRQA
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHC
GLPPYAYQVLVTAEHAQLERALAFLQAARACADASPMAAEVQLHDPVPMSMVRLFNKERA
CCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
QMLVEAGSRPLLQRFVSEWVRTFDDAATRVKGVRWQLEIDPLRI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]