BacMap

Definition	Cupriavidus metallidurans CH34 chromosome, complete genome.
Accession	NC_007973
Length	3,928,089

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The map label for this gene is echA8 [H]

Identifier: 94312344

GI number: 94312344

Start: 3693372

End: 3694142

Strand: Reverse

Name: echA8 [H]

Synonym: Rmet_3413

Alternate gene names: 94312344

Gene position: 3694142-3693372 (Counterclockwise)

Preceding gene: 94312345

Following gene: 94312343

Centisome position: 94.04

GC content: 65.11

Gene sequence:

>771_bases
ATGACATCCGTTTCCTACGAGGTCCGCGACAACATCGCGGAGATCCTTCTTGATAGCCCTCCCGTCAACGCGCTGAACGA
GGCGATGATCGACGCGTTGCTGCAAGGGCTGCGCCGAGCAGCCGAGGACGACTCGGTACGCGCCGTGATACTGGCCAGCG
CCGTGCCACGCCGGTTCTGTGCCGGGCTGCAACTCGATAGCCTGCGCGACGCGCCGCCATCGCGCGGCTACGCACTGGTG
GAGAAGCTCTATAGCGGGCTGTGCGAGGCACAGTTCCATCTTGGCAAGCCGTCGATCGCCGCCGTCGGCGGTACCGCCCG
GGGCGGTGGCATGACACTGGCGATCCACTGCGACATGATCGTTGCCGCTGACACCGCGACCTTCGGCTATCCGGAGATCG
ATGTCGGGCTGGTACCCGCGATTCACTACACCCATCTGCCGCGCATCATCGGCCGGCATCGTGCCTTCGATCTGCTGTTC
ACGGGGCGTTCGTTCGATGCGGCGGAAGCCATGTCGTTGGGGCTGGTCAACCGCGTGGTGCCAGAACAGCGCGTCATGGA
GGAGGCTCGCGCGCTGGCGCGCTCCCTGTGCCAGAAATCGCCCGAGATCATGCGGATGGGGCGGCAGGCATTCATGCGGG
CTAACGACATCGACTACCGGCGTGGCGTGGCAGGCGCGGTGGAAACGTTCGGGAACATCTTTGCTACCGAGGACGCGCAG
GAAGGCATCGCCGCATTCGTGGAGCGACGCAAGCCACGCTGGCAAGCGTGA

Upstream 100 bases:

>100_bases
ATTGCCGATTTCAAGGCGCGAGGCGTGATCGCCACCGCCAACCTGCCGCAGGCTGATTCCCAGCCGGCCGCGGCCCCTCT
CACTGCATGAGTGGAACCCC

Downstream 100 bases:

>100_bases
AGACCATGACGATGCGTGATCCTGGAACATCAGATGGGGCGCAAGACAGCGACGAGGCATTCCGCGCCGAGATCCGCTCC
TTTCTTAGGAATGCCGTGCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTSVSYEVRDNIAEILLDSPPVNALNEAMIDALLQGLRRAAEDDSVRAVILASAVPRRFCAGLQLDSLRDAPPSRGYALV
EKLYSGLCEAQFHLGKPSIAAVGGTARGGGMTLAIHCDMIVAADTATFGYPEIDVGLVPAIHYTHLPRIIGRHRAFDLLF
TGRSFDAAEAMSLGLVNRVVPEQRVMEEARALARSLCQKSPEIMRMGRQAFMRANDIDYRRGVAGAVETFGNIFATEDAQ
EGIAAFVERRKPRWQA

Sequences:

>Translated_256_residues
MTSVSYEVRDNIAEILLDSPPVNALNEAMIDALLQGLRRAAEDDSVRAVILASAVPRRFCAGLQLDSLRDAPPSRGYALV
EKLYSGLCEAQFHLGKPSIAAVGGTARGGGMTLAIHCDMIVAADTATFGYPEIDVGLVPAIHYTHLPRIIGRHRAFDLLF
TGRSFDAAEAMSLGLVNRVVPEQRVMEEARALARSLCQKSPEIMRMGRQAFMRANDIDYRRGVAGAVETFGNIFATEDAQ
EGIAAFVERRKPRWQA
>Mature_255_residues
TSVSYEVRDNIAEILLDSPPVNALNEAMIDALLQGLRRAAEDDSVRAVILASAVPRRFCAGLQLDSLRDAPPSRGYALVE
KLYSGLCEAQFHLGKPSIAAVGGTARGGGMTLAIHCDMIVAADTATFGYPEIDVGLVPAIHYTHLPRIIGRHRAFDLLFT
GRSFDAAEAMSLGLVNRVVPEQRVMEEARALARSLCQKSPEIMRMGRQAFMRANDIDYRRGVAGAVETFGNIFATEDAQE
GIAAFVERRKPRWQA

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=32.1428571428571, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=29.718875502008, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI4502327, Length=238, Percent_Identity=32.3529411764706, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI68989263, Length=282, Percent_Identity=28.7234042553192, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI150378541, Length=251, Percent_Identity=30.2788844621514, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI20127408, Length=200, Percent_Identity=28, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI62530384, Length=250, Percent_Identity=29.6, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI45643119, Length=213, Percent_Identity=28.169014084507, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI260275230, Length=213, Percent_Identity=28.169014084507, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI260274832, Length=213, Percent_Identity=28.169014084507, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI25453481, Length=249, Percent_Identity=25.7028112449799, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI51538573, Length=249, Percent_Identity=25.7028112449799, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI70995211, Length=244, Percent_Identity=28.2786885245902, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI295842266, Length=247, Percent_Identity=29.1497975708502, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI4757966, Length=213, Percent_Identity=27.6995305164319, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI51538581, Length=213, Percent_Identity=27.6995305164319, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI221136756, Length=250, Percent_Identity=26, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136753, Length=250, Percent_Identity=26, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757968, Length=249, Percent_Identity=25.3012048192771, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI49355787, Length=249, Percent_Identity=25.3012048192771, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221307494, Length=250, Percent_Identity=26, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI116235487, Length=181, Percent_Identity=33.1491712707182, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI221142681, Length=251, Percent_Identity=33.0677290836653, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787659, Length=243, Percent_Identity=33.7448559670782, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=32.4, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=32.1782178217822, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082183, Length=254, Percent_Identity=27.1653543307087, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=30.9160305343511, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1790281, Length=196, Percent_Identity=27.0408163265306, Blast_Score=67, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=31.7269076305221, Blast_Score=133, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=32.4, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17540306, Length=250, Percent_Identity=29.2, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=246, Percent_Identity=29.6747967479675, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=212, Percent_Identity=28.3018867924528, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17558304, Length=185, Percent_Identity=29.1891891891892, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=226, Percent_Identity=31.4159292035398, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=225, Percent_Identity=27.5555555555556, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=188, Percent_Identity=27.6595744680851, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=188, Percent_Identity=27.6595744680851, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=188, Percent_Identity=27.6595744680851, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=209, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=212, Percent_Identity=28.3018867924528, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=220, Percent_Identity=29.0909090909091, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24653139, Length=253, Percent_Identity=33.596837944664, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI20129971, Length=254, Percent_Identity=31.496062992126, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24653477, Length=254, Percent_Identity=31.496062992126, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922422, Length=248, Percent_Identity=26.6129032258064, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19920382, Length=237, Percent_Identity=26.5822784810127, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24583165, Length=258, Percent_Identity=25.1937984496124, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357171, Length=261, Percent_Identity=26.0536398467433, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921018, Length=256, Percent_Identity=23.4375, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24650670, Length=184, Percent_Identity=28.804347826087, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27916; Mature: 27785

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVSYEVRDNIAEILLDSPPVNALNEAMIDALLQGLRRAAEDDSVRAVILASAVPRRFC
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AGLQLDSLRDAPPSRGYALVEKLYSGLCEAQFHLGKPSIAAVGGTARGGGMTLAIHCDMI
CCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEEEE
VAADTATFGYPEIDVGLVPAIHYTHLPRIIGRHRAFDLLFTGRSFDAAEAMSLGLVNRVV
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHC
PEQRVMEEARALARSLCQKSPEIMRMGRQAFMRANDIDYRRGVAGAVETFGNIFATEDAQ
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHH
EGIAAFVERRKPRWQA
HHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TSVSYEVRDNIAEILLDSPPVNALNEAMIDALLQGLRRAAEDDSVRAVILASAVPRRFC
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AGLQLDSLRDAPPSRGYALVEKLYSGLCEAQFHLGKPSIAAVGGTARGGGMTLAIHCDMI
CCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEEEEE
VAADTATFGYPEIDVGLVPAIHYTHLPRIIGRHRAFDLLFTGRSFDAAEAMSLGLVNRVV
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHC
PEQRVMEEARALARSLCQKSPEIMRMGRQAFMRANDIDYRRGVAGAVETFGNIFATEDAQ
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHH
EGIAAFVERRKPRWQA
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]