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The map label for this gene is ygiD [H]

Identifier: 94312332

GI number: 94312332

Start: 3679499

End: 3680389

Strand: Reverse

Name: ygiD [H]

Synonym: Rmet_3401

Alternate gene names: 94312332

Gene position: 3680389-3679499 (Counterclockwise)

Preceding gene: 94312337

Following gene: 94312326

Centisome position: 93.69

GC content: 68.8

Gene sequence:

>891_bases
ATGAACACCATGCTTCCCGTCCTCTACGTGTCCCACGGATCCCCCATGCTCGCGATCGACCCAGGTCCGACCGGCGCCGC
CTTCGACGCGCTCGGCGTCCGCCTGCGCGCGCTCAAGCCCAAGGCCGTGCTGGTGATTTCCGCGCACTGGATCTACAGCA
CGCTGGCGGTCAGCACGCGCAGCCAGCAGGAGGCATGGCACGACTTCGGCGGCTTTCCGCGCGAGCTCTACACGCTGCGC
TATGACGCGCCGGGCTCGCCCGAGCTTGCCGGCCGCGTGAAGGCGCTGGTCGAGGCGGCTCAGATCCCGGGCGCCGGGTT
TGTTTGCGAGGATGAAGAGCGTCCGCTCGATCACGGAGCCTGGATGCCGCTGCGTCACTTCTTCCCGGATGCCGACGTTC
CCGTGGTCCAGCTCGCTTTCAACCCGTACCTGCCTCCGGCTACGCAGATCGAGATCGGCCGCGCACTGGCGCCGCTGCGC
GAGGAAGGCGTGCTTGTGATCGGTTCGGGCAGCTTCACGCATAACCTGCGCGAAGTGTTTGGGGCGGGTGAACGCAAGGC
GCAGCACGGCCCGGCGACCGAGCCGTACGTCGATGCCTTCCGCGACTGGATGAGCGGCGCGCTGGACGAGGCGCTGGCCA
CCGGCGACGTCAGCCGCATCGCGGACTATCGTGCCCAGGCACCGTTCGCCCGTCGCGCGCATCCGACGGACGAGCACCTG
TTGCCGTTCTACGTGGCGCTGGGCGCCGCGATCGGGCCAGCCTCCGTGGCCGAAGGGCCAGCGCGGGTGGACCGCGTGGC
CGATGAGGTCACCTATGGCGTGCTGGCGATGGACACGTTCGTCTTCGATGGCATCGGCACAGGTCATGCGCCGCTGTCGA
AGGCGGCCTGA

Upstream 100 bases:

>100_bases
GAAGTTTTTTGCACGAGGTGTCCGTAATTGGCCTGCGTCAGCGCCAGCTTTCTTTGCCAGACTTGAGGCATCGCACAAAT
TCGCTGAACGGCAACTCGCC

Downstream 100 bases:

>100_bases
GCCTGCCGCGTGTCGAATCAGGTGATGAGATAGCGGCGTGCCGCCTTCGCGCCGAACAGGGCGATATAGCCCATCGTTAG
CGCCACCACGGTGATGCCGA

Product: extradiol ring-cleavage dioxygenase, class III enzyme, subunit B (catalytic subunit)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MNTMLPVLYVSHGSPMLAIDPGPTGAAFDALGVRLRALKPKAVLVISAHWIYSTLAVSTRSQQEAWHDFGGFPRELYTLR
YDAPGSPELAGRVKALVEAAQIPGAGFVCEDEERPLDHGAWMPLRHFFPDADVPVVQLAFNPYLPPATQIEIGRALAPLR
EEGVLVIGSGSFTHNLREVFGAGERKAQHGPATEPYVDAFRDWMSGALDEALATGDVSRIADYRAQAPFARRAHPTDEHL
LPFYVALGAAIGPASVAEGPARVDRVADEVTYGVLAMDTFVFDGIGTGHAPLSKAA

Sequences:

>Translated_296_residues
MNTMLPVLYVSHGSPMLAIDPGPTGAAFDALGVRLRALKPKAVLVISAHWIYSTLAVSTRSQQEAWHDFGGFPRELYTLR
YDAPGSPELAGRVKALVEAAQIPGAGFVCEDEERPLDHGAWMPLRHFFPDADVPVVQLAFNPYLPPATQIEIGRALAPLR
EEGVLVIGSGSFTHNLREVFGAGERKAQHGPATEPYVDAFRDWMSGALDEALATGDVSRIADYRAQAPFARRAHPTDEHL
LPFYVALGAAIGPASVAEGPARVDRVADEVTYGVLAMDTFVFDGIGTGHAPLSKAA
>Mature_296_residues
MNTMLPVLYVSHGSPMLAIDPGPTGAAFDALGVRLRALKPKAVLVISAHWIYSTLAVSTRSQQEAWHDFGGFPRELYTLR
YDAPGSPELAGRVKALVEAAQIPGAGFVCEDEERPLDHGAWMPLRHFFPDADVPVVQLAFNPYLPPATQIEIGRALAPLR
EEGVLVIGSGSFTHNLREVFGAGERKAQHGPATEPYVDAFRDWMSGALDEALATGDVSRIADYRAQAPFARRAHPTDEHL
LPFYVALGAAIGPASVAEGPARVDRVADEVTYGVLAMDTFVFDGIGTGHAPLSKAA

Specific function: Unknown

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DODA-type extradiol aromatic ring- opening dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI226510974, Length=253, Percent_Identity=35.5731225296443, Blast_Score=142, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014436
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: NA

Molecular weight: Translated: 31776; Mature: 31776

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTMLPVLYVSHGSPMLAIDPGPTGAAFDALGVRLRALKPKAVLVISAHWIYSTLAVSTR
CCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHEEEEECCCEEEEEEHHHHHHHHHHCCC
SQQEAWHDFGGFPRELYTLRYDAPGSPELAGRVKALVEAAQIPGAGFVCEDEERPLDHGA
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
WMPLRHFFPDADVPVVQLAFNPYLPPATQIEIGRALAPLREEGVLVIGSGSFTHNLREVF
CCCHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCHHHCCEEEEECCCHHHHHHHHH
GAGERKAQHGPATEPYVDAFRDWMSGALDEALATGDVSRIADYRAQAPFARRAHPTDEHL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHCCCCCCCHH
LPFYVALGAAIGPASVAEGPARVDRVADEVTYGVLAMDTFVFDGIGTGHAPLSKAA
HHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNTMLPVLYVSHGSPMLAIDPGPTGAAFDALGVRLRALKPKAVLVISAHWIYSTLAVSTR
CCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHEEEEECCCEEEEEEHHHHHHHHHHCCC
SQQEAWHDFGGFPRELYTLRYDAPGSPELAGRVKALVEAAQIPGAGFVCEDEERPLDHGA
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
WMPLRHFFPDADVPVVQLAFNPYLPPATQIEIGRALAPLREEGVLVIGSGSFTHNLREVF
CCCHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCHHHCCEEEEECCCHHHHHHHHH
GAGERKAQHGPATEPYVDAFRDWMSGALDEALATGDVSRIADYRAQAPFARRAHPTDEHL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHCCCCCCCHH
LPFYVALGAAIGPASVAEGPARVDRVADEVTYGVLAMDTFVFDGIGTGHAPLSKAA
HHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1314093; 9278503 [H]