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The map label for this gene is tnpB [H]

Identifier: 94312295

GI number: 94312295

Start: 3639583

End: 3640368

Strand: Reverse

Name: tnpB [H]

Synonym: Rmet_3364

Alternate gene names: 94312295

Gene position: 3640368-3639583 (Counterclockwise)

Preceding gene: 94312296

Following gene: 94312294

Centisome position: 92.68

GC content: 60.05

Gene sequence:

>786_bases
ATGCAGCATGAAGCCATGATGGAGATGCTCAAATCCTTGAAGCTGCACGGCATGGCTCACGCGCTTGGCGACCTGGCGGC
GCAGGACTCGCCGGCCTATCAGTCCGCCGTCGCCATTCTGTCTCGCCTGCTCAAGGCAGAAACCACTGAGCGTGAAGTGC
GCTCGCTGGCCTATCAGATGAAGGTGGCGCGCTTTCCGGCCTACCGGGATCTGACCGGCTTCGACTTCAGTTGCAGCGAG
GCCAACGAGGCGCTGGTGCGGCAACTCCACCGCTGCGAGTTCCTAGAATCCGCCCACAACGTGGTGCTGGTCGGCGGCCC
TGGCACGGGCAAGACGCATCTGGGTACCGCCATCGGTGTACAGGCCGTGGAGCATCACCACCGCCGGGTGCGCTTCTTCT
CGACCGTCGAACTGGTCAACGCACTGGAACTGGAGAAGGTCTCTGGCAAGCCCGGCCATCTGGCCACGCGACTGATGTAC
GCAGACCTGGTGATCCTCGATGAGTTGGGCTATCTGCCCTTCAGCCAGGTCGGTGGCGCATTGCTGTTTCATCTGATCAG
CAAGCTCTACGAGCGCACCAGCCTGATCATCACCACCAACCTCAGCTTCGGGGAATGGTCCAGTGTCTTTGGGGATGCCA
AGATGACCACTGCGCTGCTCGACCGCCTTACCCATCACTGCCACATTGTGGAGACCGGAAACGATAGCTATCGTTTCAAG
CACAGCACCACCCAACCAAAAAAGGAGAAACGCACCGCAAAACAGCCCGCACAAAGCGACACCTAA

Upstream 100 bases:

>100_bases
CCGGTAGCCCCCATCACCACGCCTCAGGCGCTGGCTTTGCAGGTCGAGCCGCAGGCCAACGTCGCGCGTTATGACAGTCT
GCGTGACCGGGAGGTGCCGC

Downstream 100 bases:

>100_bases
CAGCTTTTGGGGTGGGTCAATTTTCGATGCAAATCGTGGGTCAGGATTCGGTGCAAATCAACATCCCAAGCTATCGAAGA
AGAACCTTGAGCGAGCCGCG

Product: IstB-like ATP binding protein ISRme4

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MQHEAMMEMLKSLKLHGMAHALGDLAAQDSPAYQSAVAILSRLLKAETTEREVRSLAYQMKVARFPAYRDLTGFDFSCSE
ANEALVRQLHRCEFLESAHNVVLVGGPGTGKTHLGTAIGVQAVEHHHRRVRFFSTVELVNALELEKVSGKPGHLATRLMY
ADLVILDELGYLPFSQVGGALLFHLISKLYERTSLIITTNLSFGEWSSVFGDAKMTTALLDRLTHHCHIVETGNDSYRFK
HSTTQPKKEKRTAKQPAQSDT

Sequences:

>Translated_261_residues
MQHEAMMEMLKSLKLHGMAHALGDLAAQDSPAYQSAVAILSRLLKAETTEREVRSLAYQMKVARFPAYRDLTGFDFSCSE
ANEALVRQLHRCEFLESAHNVVLVGGPGTGKTHLGTAIGVQAVEHHHRRVRFFSTVELVNALELEKVSGKPGHLATRLMY
ADLVILDELGYLPFSQVGGALLFHLISKLYERTSLIITTNLSFGEWSSVFGDAKMTTALLDRLTHHCHIVETGNDSYRFK
HSTTQPKKEKRTAKQPAQSDT
>Mature_261_residues
MQHEAMMEMLKSLKLHGMAHALGDLAAQDSPAYQSAVAILSRLLKAETTEREVRSLAYQMKVARFPAYRDLTGFDFSCSE
ANEALVRQLHRCEFLESAHNVVLVGGPGTGKTHLGTAIGVQAVEHHHRRVRFFSTVELVNALELEKVSGKPGHLATRLMY
ADLVILDELGYLPFSQVGGALLFHLISKLYERTSLIITTNLSFGEWSSVFGDAKMTTALLDRLTHHCHIVETGNDSYRFK
HSTTQPKKEKRTAKQPAQSDT

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 29060; Mature: 29060

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHEAMMEMLKSLKLHGMAHALGDLAAQDSPAYQSAVAILSRLLKAETTEREVRSLAYQM
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KVARFPAYRDLTGFDFSCSEANEALVRQLHRCEFLESAHNVVLVGGPGTGKTHLGTAIGV
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
QAVEHHHRRVRFFSTVELVNALELEKVSGKPGHLATRLMYADLVILDELGYLPFSQVGGA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LLFHLISKLYERTSLIITTNLSFGEWSSVFGDAKMTTALLDRLTHHCHIVETGNDSYRFK
HHHHHHHHHHHHCEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHEEEEECCCCCEEEE
HSTTQPKKEKRTAKQPAQSDT
CCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQHEAMMEMLKSLKLHGMAHALGDLAAQDSPAYQSAVAILSRLLKAETTEREVRSLAYQM
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KVARFPAYRDLTGFDFSCSEANEALVRQLHRCEFLESAHNVVLVGGPGTGKTHLGTAIGV
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
QAVEHHHRRVRFFSTVELVNALELEKVSGKPGHLATRLMYADLVILDELGYLPFSQVGGA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LLFHLISKLYERTSLIITTNLSFGEWSSVFGDAKMTTALLDRLTHHCHIVETGNDSYRFK
HHHHHHHHHHHHCEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHEEEEECCCCCEEEE
HSTTQPKKEKRTAKQPAQSDT
CCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]