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The map label for this gene is yrbE [H]

Identifier: 94312189

GI number: 94312189

Start: 3528320

End: 3529096

Strand: Reverse

Name: yrbE [H]

Synonym: Rmet_3258

Alternate gene names: 94312189

Gene position: 3529096-3528320 (Counterclockwise)

Preceding gene: 94312190

Following gene: 94312188

Centisome position: 89.84

GC content: 63.84

Gene sequence:

>777_bases
GTGAACGGCTTCTTTACCTCGATTGGCTCGACGGTACGCCAGTTCGTCGGCGGCCTGGGTCATGCCACGCGCATGTTCCT
GACGATGCTGGCGCTCTCGCCCGCGCTGTTGCGCCGCTTCCGGCTGGTGACCGACCAGGTGTTCTTCGTCGGCAACCTTT
CGCTCGTGATCATCGCGGTGTCGGGTTTGTTCGTCGGATTCGTGCTGGGTCTGCAGGGCTACTACACGCTGAACCGCTTC
GGTTCGGAGCAGGCGCTGGGCCTTCTGGTAGCCCTGTCGCTGGTGCGCGAGCTGGGGCCGGTGGTGGCAGCGCTGCTGTT
CGCCGGCCGCGCTGGCACGTCGCTGACGGCCGAAATCGGTCTGATGAAGGCCGGTGAGCAACTCAGCGCCATGGAGATGA
TGGCCGTGAACCCGTTGCAGCGCGTGATCGCACCGCGTTTCTGGGCCGGCGTGATCGCCATGCCTGTGCTGGCCACGATC
TTCAGCGCCCTGGGTGTCCTGGGTGGCTACCTGGTCGGCGTGCAACTGATTGGTGTGGACGCCGGCGCATTCTGGTCGCA
GATGCAGAACGGCGTCGATGTGCGCGCGGATGTGCTCAACGGCGTGATCAAGAGCTTTATTTTCGGTATCACCGTGACGT
TCATCGCGCTGTATCAAGGTTTCGAAGCCAAGCCGACCCCGGAGGGCGTATCGCGCGCCACCACACGTACCGTGGTACTG
GCCTCGCTGGCCGTGCTGGGACTGGATTTCCTGCTGACCGCGCTGATGTTCAGCTAG

Upstream 100 bases:

>100_bases
CATTCGTGCGCCAGTTCGTTCACGCCGAGGTGGACGGTCCGGTGCCGTTCCACTATGCCGGTCCGGCACTGGCCGATGAT
TTCGGCGTAGGAGGCGCGAA

Downstream 100 bases:

>100_bases
GCCAGCCGCACCGATCGCATGAGAAAGAGAATCGCATGAAGAAGACTACGCTCGACTACTGGGTAGGGGTGTTCGTGGTA
CTGGGCTTTGCCGCGCTGCT

Product: toluene ABC transporter membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNGFFTSIGSTVRQFVGGLGHATRMFLTMLALSPALLRRFRLVTDQVFFVGNLSLVIIAVSGLFVGFVLGLQGYYTLNRF
GSEQALGLLVALSLVRELGPVVAALLFAGRAGTSLTAEIGLMKAGEQLSAMEMMAVNPLQRVIAPRFWAGVIAMPVLATI
FSALGVLGGYLVGVQLIGVDAGAFWSQMQNGVDVRADVLNGVIKSFIFGITVTFIALYQGFEAKPTPEGVSRATTRTVVL
ASLAVLGLDFLLTALMFS

Sequences:

>Translated_258_residues
MNGFFTSIGSTVRQFVGGLGHATRMFLTMLALSPALLRRFRLVTDQVFFVGNLSLVIIAVSGLFVGFVLGLQGYYTLNRF
GSEQALGLLVALSLVRELGPVVAALLFAGRAGTSLTAEIGLMKAGEQLSAMEMMAVNPLQRVIAPRFWAGVIAMPVLATI
FSALGVLGGYLVGVQLIGVDAGAFWSQMQNGVDVRADVLNGVIKSFIFGITVTFIALYQGFEAKPTPEGVSRATTRTVVL
ASLAVLGLDFLLTALMFS
>Mature_258_residues
MNGFFTSIGSTVRQFVGGLGHATRMFLTMLALSPALLRRFRLVTDQVFFVGNLSLVIIAVSGLFVGFVLGLQGYYTLNRF
GSEQALGLLVALSLVRELGPVVAALLFAGRAGTSLTAEIGLMKAGEQLSAMEMMAVNPLQRVIAPRFWAGVIAMPVLATI
FSALGVLGGYLVGVQLIGVDAGAFWSQMQNGVDVRADVLNGVIKSFIFGITVTFIALYQGFEAKPTPEGVSRATTRTVVL
ASLAVLGLDFLLTALMFS

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=248, Percent_Identity=61.2903225806452, Blast_Score=294, Evalue=4e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27347; Mature: 27347

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]