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The map label for this gene is ubiF [H]

Identifier: 94312122

GI number: 94312122

Start: 3466172

End: 3467596

Strand: Reverse

Name: ubiF [H]

Synonym: Rmet_3191

Alternate gene names: 94312122

Gene position: 3467596-3466172 (Counterclockwise)

Preceding gene: 94312125

Following gene: 94312121

Centisome position: 88.28

GC content: 68.84

Gene sequence:

>1425_bases
ATGCCGGCTTCGGGCGGTCGGGTGCGCCGGCCGGGAGGCAAGCGGCAGAACCGGTCCGTATGCGTGTCCGAACCGATCAC
GCGGATGGAGCCCCCGTCACGTCGCTATAATTCAGGGATGTCCGGACCTGCCGCATCGGTGTCACGCACCGTCACTTCTT
CCATGTCCTCGAATTCCTCTTCCCGCTTCCAGGTCGCCGTGGTCGGCGGCGGCATTGTTGGCAAGGCCTGCGCGCTGCTG
CTGGCGCAGCAGGGTATGCAGGTGGCGCTGGCGGCGCCCCGGCCCGCTGCTGAGGGGGCCCGCCGGGGGGGCGATGACTG
GGATGCCCGCATCTACGCGTTTTCCGCCAGTTCGCAGGCGCTGCTGGAGCGGCTGCGTGTCTGGGAGGCGCTGGACCCGG
CGCGTGTGCAGCCCGTGCGCGACATGCGTGTGTTTGGTGATGAGAGTGCTGCCGAGACCGATCCCTCGCTCGATGGGGAT
CTGCATTTCTCGGCCTACGCCGCCGCCGTGCCGCAGCTCGCGTGGATCGTCGAATCTGGGCACGTCGAGCGCGTGCTCGA
CACCGCGCTGCGATTCCAGCACCAGGTGAAATGGTTCGACGACACGGCCACCGGGCTCGAGCGCGACGTCGACGGTGTGA
CGCTGACGCTGGCCAGCGGCGCACAAGTTCGCGCCAATTTGTTGGTTGGGGCCGACGGGTCCCGGTCCTGGGTGCGCCAG
CAATGTCATATCGGCACCACGCAGCGGCGGTATCGGCAGCTTGGCGTGGTGGCCAACTTCCAGTGCGAACTGCCGCACCA
GGAAACCGCCTGGCAATGGTTCCTGGGGGCGCCGGAGAAGTTGATGGCTGACGAGGAACCGGCCAACGGCGAAATCCTGG
CGATGCTGCCGTTGCCGGGCAATCGGGTGTCGATGGTGTGGTCGGCCGATGAGGCACACGCCCACGACCTCCTCGCGTTG
TCGCCCGAGGCGCTGGCCGCGACCGTGGCCCAGGTAGCCAGCGGCGCGGTGGGCGCCCGCTTTGGCGCACTGCGCTGCAT
CACGCCGGCGCAGGGCTTCCCGCTGGTGCTGCAGCGCGCCGAGCAGTTCGTCCAGCCGCACGTGGCGCTGGTCGGCGATG
CCGCGCACGTGGTCCATCCGCTCGCCGGGCAGGGCATGAACCTGGGTTTGCGCGATGTGGTGGAACTCGGGCGGGTGATG
GCCGACAAAGAGTCGTTCCGTTCGGAAGGGGATTTGCGCCTGTTGCGCCGCTACGAGCGCGCGCGCGCTACCGATTTGCT
CTCGCTGACCGCCGCCACCGATGGCTTGCACCGGTTGTTCTCGCTGCCCGGCACGCCGGCGCGTGTGGTGCGCAATCTGG
GGATGCGGGCGGTGGGCAGCCAGGGGCTGATCAAGCGGCTCCTGATCGGCCACGCGCTCGGGTGA

Upstream 100 bases:

>100_bases
TGACCTCGACGTGGGGCATTTTCGATGGCACTCGCTTATTGCTTGCGGGATAAGAAGAAAATGACCTGCGGACCGGACGG
CGCAGGTCGCCTTGACGGCA

Downstream 100 bases:

>100_bases
TGCAAGCCGGAAGAGGCGAATCCACAAACGAGCCACAAACCAGCCCATTAACGATTTACCATTCACGAACAAGCCGGCAC
ACTGACCGGCGCATCGTTTT

Product: ubiquinone biosynthesis hydroxylase family protein

Products: 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MPASGGRVRRPGGKRQNRSVCVSEPITRMEPPSRRYNSGMSGPAASVSRTVTSSMSSNSSSRFQVAVVGGGIVGKACALL
LAQQGMQVALAAPRPAAEGARRGGDDWDARIYAFSASSQALLERLRVWEALDPARVQPVRDMRVFGDESAAETDPSLDGD
LHFSAYAAAVPQLAWIVESGHVERVLDTALRFQHQVKWFDDTATGLERDVDGVTLTLASGAQVRANLLVGADGSRSWVRQ
QCHIGTTQRRYRQLGVVANFQCELPHQETAWQWFLGAPEKLMADEEPANGEILAMLPLPGNRVSMVWSADEAHAHDLLAL
SPEALAATVAQVASGAVGARFGALRCITPAQGFPLVLQRAEQFVQPHVALVGDAAHVVHPLAGQGMNLGLRDVVELGRVM
ADKESFRSEGDLRLLRRYERARATDLLSLTAATDGLHRLFSLPGTPARVVRNLGMRAVGSQGLIKRLLIGHALG

Sequences:

>Translated_474_residues
MPASGGRVRRPGGKRQNRSVCVSEPITRMEPPSRRYNSGMSGPAASVSRTVTSSMSSNSSSRFQVAVVGGGIVGKACALL
LAQQGMQVALAAPRPAAEGARRGGDDWDARIYAFSASSQALLERLRVWEALDPARVQPVRDMRVFGDESAAETDPSLDGD
LHFSAYAAAVPQLAWIVESGHVERVLDTALRFQHQVKWFDDTATGLERDVDGVTLTLASGAQVRANLLVGADGSRSWVRQ
QCHIGTTQRRYRQLGVVANFQCELPHQETAWQWFLGAPEKLMADEEPANGEILAMLPLPGNRVSMVWSADEAHAHDLLAL
SPEALAATVAQVASGAVGARFGALRCITPAQGFPLVLQRAEQFVQPHVALVGDAAHVVHPLAGQGMNLGLRDVVELGRVM
ADKESFRSEGDLRLLRRYERARATDLLSLTAATDGLHRLFSLPGTPARVVRNLGMRAVGSQGLIKRLLIGHALG
>Mature_473_residues
PASGGRVRRPGGKRQNRSVCVSEPITRMEPPSRRYNSGMSGPAASVSRTVTSSMSSNSSSRFQVAVVGGGIVGKACALLL
AQQGMQVALAAPRPAAEGARRGGDDWDARIYAFSASSQALLERLRVWEALDPARVQPVRDMRVFGDESAAETDPSLDGDL
HFSAYAAAVPQLAWIVESGHVERVLDTALRFQHQVKWFDDTATGLERDVDGVTLTLASGAQVRANLLVGADGSRSWVRQQ
CHIGTTQRRYRQLGVVANFQCELPHQETAWQWFLGAPEKLMADEEPANGEILAMLPLPGNRVSMVWSADEAHAHDLLALS
PEALAATVAQVASGAVGARFGALRCITPAQGFPLVLQRAEQFVQPHVALVGDAAHVVHPLAGQGMNLGLRDVVELGRVMA
DKESFRSEGDLRLLRRYERARATDLLSLTAATDGLHRLFSLPGTPARVVRNLGMRAVGSQGLIKRLLIGHALG

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=424, Percent_Identity=27.5943396226415, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI32967307, Length=292, Percent_Identity=31.8493150684932, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1786883, Length=418, Percent_Identity=34.2105263157895, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1789273, Length=430, Percent_Identity=31.1627906976744, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789274, Length=403, Percent_Identity=29.7766749379653, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25146631, Length=465, Percent_Identity=27.741935483871, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19920760, Length=473, Percent_Identity=29.3868921775898, Blast_Score=122, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 50956; Mature: 50824

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASGGRVRRPGGKRQNRSVCVSEPITRMEPPSRRYNSGMSGPAASVSRTVTSSMSSNSS
CCCCCCCEECCCCCCCCCCEECCCCHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCC
SRFQVAVVGGGIVGKACALLLAQQGMQVALAAPRPAAEGARRGGDDWDARIYAFSASSQA
CEEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCCCCCEEEEECCCHHH
LLERLRVWEALDPARVQPVRDMRVFGDESAAETDPSLDGDLHFSAYAAAVPQLAWIVESG
HHHHHHHHHCCCCHHCCCHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCC
HVERVLDTALRFQHQVKWFDDTATGLERDVDGVTLTLASGAQVRANLLVGADGSRSWVRQ
CHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCEEEEEEEEECCCCHHHHHH
QCHIGTTQRRYRQLGVVANFQCELPHQETAWQWFLGAPEKLMADEEPANGEILAMLPLPG
HHCCCHHHHHHHHHCCEEEEEECCCCCCCCHHHCCCCCHHHHCCCCCCCCCEEEEEECCC
NRVSMVWSADEAHAHDLLALSPEALAATVAQVASGAVGARFGALRCITPAQGFPLVLQRA
CCEEEEEECCCCCCCHHEEECHHHHHHHHHHHHCCCHHHHHCCEEEECCCCCCHHHHHHH
EQFVQPHVALVGDAAHVVHPLAGQGMNLGLRDVVELGRVMADKESFRSEGDLRLLRRYER
HHHHCCCCEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ARATDLLSLTAATDGLHRLFSLPGTPARVVRNLGMRAVGSQGLIKRLLIGHALG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PASGGRVRRPGGKRQNRSVCVSEPITRMEPPSRRYNSGMSGPAASVSRTVTSSMSSNSS
CCCCCCEECCCCCCCCCCEECCCCHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCC
SRFQVAVVGGGIVGKACALLLAQQGMQVALAAPRPAAEGARRGGDDWDARIYAFSASSQA
CEEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCCCCCEEEEECCCHHH
LLERLRVWEALDPARVQPVRDMRVFGDESAAETDPSLDGDLHFSAYAAAVPQLAWIVESG
HHHHHHHHHCCCCHHCCCHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCC
HVERVLDTALRFQHQVKWFDDTATGLERDVDGVTLTLASGAQVRANLLVGADGSRSWVRQ
CHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCEEEEEEEEECCCCHHHHHH
QCHIGTTQRRYRQLGVVANFQCELPHQETAWQWFLGAPEKLMADEEPANGEILAMLPLPG
HHCCCHHHHHHHHHCCEEEEEECCCCCCCCHHHCCCCCHHHHCCCCCCCCCEEEEEECCC
NRVSMVWSADEAHAHDLLALSPEALAATVAQVASGAVGARFGALRCITPAQGFPLVLQRA
CCEEEEEECCCCCCCHHEEECHHHHHHHHHHHHCCCHHHHHCCEEEECCCCCCHHHHHHH
EQFVQPHVALVGDAAHVVHPLAGQGMNLGLRDVVELGRVMADKESFRSEGDLRLLRRYER
HHHHCCCCEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ARATDLLSLTAATDGLHRLFSLPGTPARVVRNLGMRAVGSQGLIKRLLIGHALG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! [C]

Specific reaction: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! = 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]