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The map label for this gene is nirJ [H]

Identifier: 94312101

GI number: 94312101

Start: 3443298

End: 3444461

Strand: Reverse

Name: nirJ [H]

Synonym: Rmet_3170

Alternate gene names: 94312101

Gene position: 3444461-3443298 (Counterclockwise)

Preceding gene: 94312102

Following gene: 94312100

Centisome position: 87.69

GC content: 65.72

Gene sequence:

>1164_bases
ATGTTCCGACTCTCCCGCTTCATGGAGGCGCTGCGCGACGGGGGGTCGGTCCCCCCGCCGCGCCGTGCCAGTGGTCCCGT
GGTGATCTGGAATCTGGTGCGGCGCTGCAATCTCAACTGCAAGCACTGTTATTCCACATCTGCCGATACGGATTTCAAGG
GCGAGCTGTCGACCGGCGAAGTCATGGCCGTGCTCGACCAGCTCAAGGCGGCGCGCGTGCCCGCGCTGATCCTGTCCGGT
GGCGAACCGCTGTTGCGGCCGGACCTGTACGAGATCGCCGCGCGTGCGCGCGAACTCGGATTTCATCTCTCCCTGTCGTC
GAATGGTACGTTGCTCGACGCGGCACACGCTGCGCGGCTCGCTGCGGCAGGGTTCGACTATGTCGGCGTGAGCCTGGATG
GCATCGAAGCCACACACGATCGCTTCCGCCGCATGAATGGCGCCTTTACCCAGGCGATAGCCGGGCTGCGCATCGCGCGC
GAAGCAGGCCTGCGCGTGGGGGTGCGCATGACGCTGACCGAGGCCAACGCGGCCGAGCTGCCTTCCGTGGTGGCCCTGGC
CGAGGCCGAAGGCATCGACAAGTTCTATCTGTCCCACTTCAACTACGCCGGGCGAGGCCGCAGCCATCGCAACGATGACG
CGCGCCACGCGGGTACACGCGTGGCGGTCGAGTGGTTGTTCGAGCATGTCTGGGCGCGCGCGCAGCAGGGTAGCGCCGGG
GATTTCGTCACGGGCAACAACGATGCCGATGGCGTGTTCCTGCTGTACTGGATTGCGCGTCAATGGCCCGAGCGCGTTGC
AGACATGCGAGAGCGACTGGTCAACTGGGGCGGCAATGCCACGGGTGTCGGTGTGGCCAATATCGACAACCTCGGCAATG
TCCACCCGGACACGATGTGGTGGCAACTCTCTCTTGGCAACGTGCGCGAGCGCCCGTTCGGCGAGATCTGGGCCGACCGC
AGCCATCCGTTGATGCGTGGCTTGGCCGCCAGGCCGAGACCGTTGCAGGGCCGATGCGCAACTTGCCTGCATCGTGACAT
CTGCAACGGCAATACGCGCGCGCGGGCCTACGCGGTCAGCGGCAATCCGTGGGCGCAGGACCCGGGCTGCTATCTCTCAG
ACGCGGAGATCAGTGCGCAGCCCAATCTGGAGACAGTGACATGA

Upstream 100 bases:

>100_bases
GGGAAGTAGCGGAAAGTGGGGCCCGATCCCGATGCCGCCCAATCAGGTCAGCGAGGCGGAAGCCAACACGTTGGCCAAGT
GGGTGCTAAGCCTCTGATCC

Downstream 100 bases:

>100_bases
AGCACGCGATTCTCTGGACGTGCATGTTTGCTTCATTCGTCGCCAACGCGGAGACGACAATCGATCCCGCCAGCATCTAC
AGCAGCCACTGTGCGGCTTG

Product: Heme d1 biosynthesis protein NirJ

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MFRLSRFMEALRDGGSVPPPRRASGPVVIWNLVRRCNLNCKHCYSTSADTDFKGELSTGEVMAVLDQLKAARVPALILSG
GEPLLRPDLYEIAARARELGFHLSLSSNGTLLDAAHAARLAAAGFDYVGVSLDGIEATHDRFRRMNGAFTQAIAGLRIAR
EAGLRVGVRMTLTEANAAELPSVVALAEAEGIDKFYLSHFNYAGRGRSHRNDDARHAGTRVAVEWLFEHVWARAQQGSAG
DFVTGNNDADGVFLLYWIARQWPERVADMRERLVNWGGNATGVGVANIDNLGNVHPDTMWWQLSLGNVRERPFGEIWADR
SHPLMRGLAARPRPLQGRCATCLHRDICNGNTRARAYAVSGNPWAQDPGCYLSDAEISAQPNLETVT

Sequences:

>Translated_387_residues
MFRLSRFMEALRDGGSVPPPRRASGPVVIWNLVRRCNLNCKHCYSTSADTDFKGELSTGEVMAVLDQLKAARVPALILSG
GEPLLRPDLYEIAARARELGFHLSLSSNGTLLDAAHAARLAAAGFDYVGVSLDGIEATHDRFRRMNGAFTQAIAGLRIAR
EAGLRVGVRMTLTEANAAELPSVVALAEAEGIDKFYLSHFNYAGRGRSHRNDDARHAGTRVAVEWLFEHVWARAQQGSAG
DFVTGNNDADGVFLLYWIARQWPERVADMRERLVNWGGNATGVGVANIDNLGNVHPDTMWWQLSLGNVRERPFGEIWADR
SHPLMRGLAARPRPLQGRCATCLHRDICNGNTRARAYAVSGNPWAQDPGCYLSDAEISAQPNLETVT
>Mature_387_residues
MFRLSRFMEALRDGGSVPPPRRASGPVVIWNLVRRCNLNCKHCYSTSADTDFKGELSTGEVMAVLDQLKAARVPALILSG
GEPLLRPDLYEIAARARELGFHLSLSSNGTLLDAAHAARLAAAGFDYVGVSLDGIEATHDRFRRMNGAFTQAIAGLRIAR
EAGLRVGVRMTLTEANAAELPSVVALAEAEGIDKFYLSHFNYAGRGRSHRNDDARHAGTRVAVEWLFEHVWARAQQGSAG
DFVTGNNDADGVFLLYWIARQWPERVADMRERLVNWGGNATGVGVANIDNLGNVHPDTMWWQLSLGNVRERPFGEIWADR
SHPLMRGLAARPRPLQGRCATCLHRDICNGNTRARAYAVSGNPWAQDPGCYLSDAEISAQPNLETVT

Specific function: Together With Moac, Is Involved In The Conversion Of A Guanosine Derivative (Gxp) Into Molybdopterin Precursor Z. [C]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42457; Mature: 42457

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRLSRFMEALRDGGSVPPPRRASGPVVIWNLVRRCNLNCKHCYSTSADTDFKGELSTGE
CCCHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHH
VMAVLDQLKAARVPALILSGGEPLLRPDLYEIAARARELGFHLSLSSNGTLLDAAHAARL
HHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCEEEEECCCCCEEEHHHHHHH
AAAGFDYVGVSLDGIEATHDRFRRMNGAFTQAIAGLRIAREAGLRVGVRMTLTEANAAEL
HHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHC
PSVVALAEAEGIDKFYLSHFNYAGRGRSHRNDDARHAGTRVAVEWLFEHVWARAQQGSAG
CHHEEHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DFVTGNNDADGVFLLYWIARQWPERVADMRERLVNWGGNATGVGVANIDNLGNVHPDTMW
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCEE
WQLSLGNVRERPFGEIWADRSHPLMRGLAARPRPLQGRCATCLHRDICNGNTRARAYAVS
EEEECCCCCCCCCHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
GNPWAQDPGCYLSDAEISAQPNLETVT
CCCCCCCCCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MFRLSRFMEALRDGGSVPPPRRASGPVVIWNLVRRCNLNCKHCYSTSADTDFKGELSTGE
CCCHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHH
VMAVLDQLKAARVPALILSGGEPLLRPDLYEIAARARELGFHLSLSSNGTLLDAAHAARL
HHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCEEEEECCCCCEEEHHHHHHH
AAAGFDYVGVSLDGIEATHDRFRRMNGAFTQAIAGLRIAREAGLRVGVRMTLTEANAAEL
HHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHC
PSVVALAEAEGIDKFYLSHFNYAGRGRSHRNDDARHAGTRVAVEWLFEHVWARAQQGSAG
CHHEEHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DFVTGNNDADGVFLLYWIARQWPERVADMRERLVNWGGNATGVGVANIDNLGNVHPDTMW
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCEE
WQLSLGNVRERPFGEIWADRSHPLMRGLAARPRPLQGRCATCLHRDICNGNTRARAYAVS
EEEECCCCCCCCCHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
GNPWAQDPGCYLSDAEISAQPNLETVT
CCCCCCCCCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11111029 [H]