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The map label for this gene is ddlB

Identifier: 94312057

GI number: 94312057

Start: 3388200

End: 3389183

Strand: Reverse

Name: ddlB

Synonym: Rmet_3126

Alternate gene names: 94312057

Gene position: 3389183-3388200 (Counterclockwise)

Preceding gene: 94312058

Following gene: 94312056

Centisome position: 86.28

GC content: 63.52

Gene sequence:

>984_bases
ATGAGCTTCGTCGCCCATCCCAATATCGATCCGAAGTCGCTGGGCAAGGTTGGCGTGCTGCTGGGTGGCAAGTCCGCCGA
GCGCGAGATCTCGCTGCTGTCCGGTAACGGTGTGCTGGCCGCGCTGAAGTCGCGCGGCGTCGATGCGCATCCGTTCGATC
CGGGCCTGCAGCCGATCAGCGAACTGGCTGCCGCAGGTTTCGACCGCGTTTTTATCGCGCTGCACGGTCGCTACGGCGAA
GACGGCACGATGCAGGGCCTGCTCGAGCAGCTTGGCATCCCCTATACCGGCAGCGGCGTGCTGGCGTCGGCGCTGGCCAT
GGACAAGCAGGCTACCAAACGCCTGTGGATGACGCACGGCCTGGCTACGCCGCGCTTCGCGATGCTCTACGCGAATACCG
ATTTCGATGCCGTGGTTGCCGATCTTGGCCTGCCGCTGATCGTCAAGCCGGCCCGTGAAGGGTCGTCGATCGGCCTGACC
AAGGTGATCGCCGCGGACCAGATGCGCGCTGCATTCGAGAAGGCCGCAGGGCTCGATGCCGACGTGATCGCCGAGACTTT
CATCGATGGCGCGGAGCTGACTTGCCCGATCGTGGGCGAGGGTCCGACGGCCGAAGCGCTCCCAGTTATCAAGATTGTCG
CGCCGGAGTCGAACTACGACTATCAAAATAAGTATTTTACTGACGATACCCAATACCTGTGCCCCTCGACATTGCCCGAC
GCGCTGGAGCGCGAAGTGCGCGCGCTGGCCGTGGAGGCCTTCCGGGTGCTGGGGTGTCGCGGCTGGGCCCGCGCGGACGT
GATGCTGACGCGCGACGGCAAGCCTTATCTGCTTGAAATGAATACGTCCCCCGGCATGACCGGCCATTCGCTCGTGCCGA
TGGCGGCGCGTGCGAATGGCATCAGCTATGAGGACTTCGTCATGCAGGTACTGGCGGCAGCCACGCTGGACCTGCATCCG
AATGAACACTGGAAACCCGAATAA

Upstream 100 bases:

>100_bases
TGAATGCCGTACGGCCCGGCGATGTGGTCGTAACCATGGGCGCTGGCTCGATCGGCGCAGTGCCGGGCCAGTTGGTGTCG
CATCAACAAGGGGGGCAGCA

Downstream 100 bases:

>100_bases
CCGCAGTACCGACCGCCTGAACGACGACACATGTGGCATAACGCGCGCCTGCTCAACCTGTTCGCCTCAGCGCTATACGC
GCTGGTGGCGCTCATGGCGC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSFVAHPNIDPKSLGKVGVLLGGKSAEREISLLSGNGVLAALKSRGVDAHPFDPGLQPISELAAAGFDRVFIALHGRYGE
DGTMQGLLEQLGIPYTGSGVLASALAMDKQATKRLWMTHGLATPRFAMLYANTDFDAVVADLGLPLIVKPAREGSSIGLT
KVIAADQMRAAFEKAAGLDADVIAETFIDGAELTCPIVGEGPTAEALPVIKIVAPESNYDYQNKYFTDDTQYLCPSTLPD
ALEREVRALAVEAFRVLGCRGWARADVMLTRDGKPYLLEMNTSPGMTGHSLVPMAARANGISYEDFVMQVLAAATLDLHP
NEHWKPE

Sequences:

>Translated_327_residues
MSFVAHPNIDPKSLGKVGVLLGGKSAEREISLLSGNGVLAALKSRGVDAHPFDPGLQPISELAAAGFDRVFIALHGRYGE
DGTMQGLLEQLGIPYTGSGVLASALAMDKQATKRLWMTHGLATPRFAMLYANTDFDAVVADLGLPLIVKPAREGSSIGLT
KVIAADQMRAAFEKAAGLDADVIAETFIDGAELTCPIVGEGPTAEALPVIKIVAPESNYDYQNKYFTDDTQYLCPSTLPD
ALEREVRALAVEAFRVLGCRGWARADVMLTRDGKPYLLEMNTSPGMTGHSLVPMAARANGISYEDFVMQVLAAATLDLHP
NEHWKPE
>Mature_326_residues
SFVAHPNIDPKSLGKVGVLLGGKSAEREISLLSGNGVLAALKSRGVDAHPFDPGLQPISELAAAGFDRVFIALHGRYGED
GTMQGLLEQLGIPYTGSGVLASALAMDKQATKRLWMTHGLATPRFAMLYANTDFDAVVADLGLPLIVKPAREGSSIGLTK
VIAADQMRAAFEKAAGLDADVIAETFIDGAELTCPIVGEGPTAEALPVIKIVAPESNYDYQNKYFTDDTQYLCPSTLPDA
LEREVRALAVEAFRVLGCRGWARADVMLTRDGKPYLLEMNTSPGMTGHSLVPMAARANGISYEDFVMQVLAAATLDLHPN
EHWKPE

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=307, Percent_Identity=50.1628664495114, Blast_Score=300, Evalue=9e-83,
Organism=Escherichia coli, GI1786579, Length=348, Percent_Identity=34.4827586206897, Blast_Score=179, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_RALME (Q1LIM8)

Other databases:

- EMBL:   CP000352
- RefSeq:   YP_585267.1
- ProteinModelPortal:   Q1LIM8
- SMR:   Q1LIM8
- STRING:   Q1LIM8
- GeneID:   4039955
- GenomeReviews:   CP000352_GR
- KEGG:   rme:Rmet_3126
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   EVLVEKW
- PhylomeDB:   Q1LIM8
- ProtClustDB:   PRK01372
- BioCyc:   RMET266264:RMET_3126-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 34968; Mature: 34837

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVAHPNIDPKSLGKVGVLLGGKSAEREISLLSGNGVLAALKSRGVDAHPFDPGLQPIS
CCCCCCCCCCHHHCCCEEEEECCCCCCCEEEEEECCCEEEEHHHCCCCCCCCCCCHHHHH
ELAAAGFDRVFIALHGRYGEDGTMQGLLEQLGIPYTGSGVLASALAMDKQATKRLWMTHG
HHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LATPRFAMLYANTDFDAVVADLGLPLIVKPAREGSSIGLTKVIAADQMRAAFEKAAGLDA
CCCCCEEEEEECCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
DVIAETFIDGAELTCPIVGEGPTAEALPVIKIVAPESNYDYQNKYFTDDTQYLCPSTLPD
HHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCEECCCCCCCCCCCCHH
ALEREVRALAVEAFRVLGCRGWARADVMLTRDGKPYLLEMNTSPGMTGHSLVPMAARANG
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHCCC
ISYEDFVMQVLAAATLDLHPNEHWKPE
CCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SFVAHPNIDPKSLGKVGVLLGGKSAEREISLLSGNGVLAALKSRGVDAHPFDPGLQPIS
CCCCCCCCCHHHCCCEEEEECCCCCCCEEEEEECCCEEEEHHHCCCCCCCCCCCHHHHH
ELAAAGFDRVFIALHGRYGEDGTMQGLLEQLGIPYTGSGVLASALAMDKQATKRLWMTHG
HHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LATPRFAMLYANTDFDAVVADLGLPLIVKPAREGSSIGLTKVIAADQMRAAFEKAAGLDA
CCCCCEEEEEECCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
DVIAETFIDGAELTCPIVGEGPTAEALPVIKIVAPESNYDYQNKYFTDDTQYLCPSTLPD
HHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCEECCCCCCCCCCCCHH
ALEREVRALAVEAFRVLGCRGWARADVMLTRDGKPYLLEMNTSPGMTGHSLVPMAARANG
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHCCC
ISYEDFVMQVLAAATLDLHPNEHWKPE
CCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA