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The map label for this gene is stcE [H]

Identifier: 94312028

GI number: 94312028

Start: 3355655

End: 3357076

Strand: Reverse

Name: stcE [H]

Synonym: Rmet_3097

Alternate gene names: 94312028

Gene position: 3357076-3355655 (Counterclockwise)

Preceding gene: 94312029

Following gene: 94312027

Centisome position: 85.46

GC content: 55.91

Gene sequence:

>1422_bases
ATGAGGGTGATATTACCATTGGTCGCATTGATGCTGTCATCGGCGGTTAATGCCGTGGTTATTGATGAAGATACTTTCAA
GCGAAATGGCGGCGATATGAGAAATGTCGGCGCATCCATTCGAACCGCTAACGAAAAACTACAGAAGCACAGTACTGCAA
AGCCCTGGCTGTCCGTTGGGCAACTCAATGGATGCACGGCAACCTGGCTCGGCAACAAGAATGGTTGGGCCCATATCCTG
ACAGCCGCACATTGTGTTCCCTATTCGGCAACGGAAACGCCAGTACGATTAACGTTCAGCGCCTGGGATGGCAGCGTTAT
GGCCTCCGGACTGGGGATCGTCTATGTGCCCAAGGAGCGTGTCAATGTTCCTGCCAACATGGGCGGGGCTTCGACGGATG
TCGCCATCGTAAGGCTGCCGCTGGTCAATCCGCTGAAAGACAAGGCGGGGATTCCGGTAGAAAGGCCGATTCTAAACGAC
AGGGAGGACGAGAAAGGCAAGGCTGTCATGTTTGTCGGCTATGGAGTCTGGGGTGTAGGGCTGAACCAAGGCTATGGGCC
GGAAAGCGGCACCAGGAGACTGTACGGCCGCAGCAAGATCACTTCGATCTTCGAGAGTGACTACGGCATCGGCGCAAGCT
ATAAACCGCTTGGTCCTTCAGCCAACTGGGCCCGTGTCGCGGCTGGTGATAGCGGTTCGGCATGGTGGCAGATCAAGGAT
ACGAAGCCCGTGATCGTTGCCACCACTAATGGCGGTCACGCGAGAGCCTCGACAGGCGCCCGCATCTCAAAATATGCCAA
CTGGATTCGCTCGATTTACCCGGACGCCCGCTTTCTCTCGGAGAGCCGTCCGCAGGGCTGCATCATCAGCCTGAGGAACG
GTGCAAAGTACTGCCTCGAGGCTGGGCAAAAATCGGACTACAGTCTGCCCGCCTGGATTTACGCGCACGATGTCTATGTG
CAGGCGGATAGTGGAGTGTCGGTCGTGCTGTCTGACTGGGATAACCTTTCCTATCACCGGCTGGCGGAGTTTGTCGGTAC
GGTGGAAAACGACAAACTGCGTGGCGTCAAGGCCAACAACGGAGAGACGCTGGATTTCTCAAAGCCACGATCGATGGAAG
TCAAGCACAGCACCCGCCCTCTGGGCTGCATCGTCAGTCTGGTCTCGGTCGACAAATACTGTCTTCCGGCAGGCGAGCGC
TCCGCGTATTCGCTGCCCGCCTGGATCTATGCGCACGATGTTGTTGTCCAGGCGGACGCTGGCATCGGCGTCATGCTGTC
TGACTGGGATAACCTGTCCTATAACCGATTGGCAGTTTTCAGTGACGTCGTTGAGAATGAAAATATGAAGAGGGTGCAAG
CTTACGACGGACAGACACTGGACTTTTCAAAGCCGCGTTCAATGCGGGTGGTCCAGCAATAA

Upstream 100 bases:

>100_bases
TATTTTGGATTTATTTCGATAAATCAAAAAATGGTTCGCCTAATGGCGTGATCGCCTATGGTATGGGTTTGTCAATCATA
TTGTTGCGAAAGGTCGATTT

Downstream 100 bases:

>100_bases
TAATTAGGCTGACGGCTGCCCCGTATAGCCGGTTCGTCAACTGTCATGGTGACGAGGTCGGTCAGTACCGGTCCTTTCTT
GGCAAAGTGTGCCGGTACTG

Product: hypothetical protein

Products: NA

Alternate protein names: Mucinase; Neutral zinc metalloprotease stcE; Secreted protease of C1 esterase inhibitor from EHEC [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MRVILPLVALMLSSAVNAVVIDEDTFKRNGGDMRNVGASIRTANEKLQKHSTAKPWLSVGQLNGCTATWLGNKNGWAHIL
TAAHCVPYSATETPVRLTFSAWDGSVMASGLGIVYVPKERVNVPANMGGASTDVAIVRLPLVNPLKDKAGIPVERPILND
REDEKGKAVMFVGYGVWGVGLNQGYGPESGTRRLYGRSKITSIFESDYGIGASYKPLGPSANWARVAAGDSGSAWWQIKD
TKPVIVATTNGGHARASTGARISKYANWIRSIYPDARFLSESRPQGCIISLRNGAKYCLEAGQKSDYSLPAWIYAHDVYV
QADSGVSVVLSDWDNLSYHRLAEFVGTVENDKLRGVKANNGETLDFSKPRSMEVKHSTRPLGCIVSLVSVDKYCLPAGER
SAYSLPAWIYAHDVVVQADAGIGVMLSDWDNLSYNRLAVFSDVVENENMKRVQAYDGQTLDFSKPRSMRVVQQ

Sequences:

>Translated_473_residues
MRVILPLVALMLSSAVNAVVIDEDTFKRNGGDMRNVGASIRTANEKLQKHSTAKPWLSVGQLNGCTATWLGNKNGWAHIL
TAAHCVPYSATETPVRLTFSAWDGSVMASGLGIVYVPKERVNVPANMGGASTDVAIVRLPLVNPLKDKAGIPVERPILND
REDEKGKAVMFVGYGVWGVGLNQGYGPESGTRRLYGRSKITSIFESDYGIGASYKPLGPSANWARVAAGDSGSAWWQIKD
TKPVIVATTNGGHARASTGARISKYANWIRSIYPDARFLSESRPQGCIISLRNGAKYCLEAGQKSDYSLPAWIYAHDVYV
QADSGVSVVLSDWDNLSYHRLAEFVGTVENDKLRGVKANNGETLDFSKPRSMEVKHSTRPLGCIVSLVSVDKYCLPAGER
SAYSLPAWIYAHDVVVQADAGIGVMLSDWDNLSYNRLAVFSDVVENENMKRVQAYDGQTLDFSKPRSMRVVQQ
>Mature_473_residues
MRVILPLVALMLSSAVNAVVIDEDTFKRNGGDMRNVGASIRTANEKLQKHSTAKPWLSVGQLNGCTATWLGNKNGWAHIL
TAAHCVPYSATETPVRLTFSAWDGSVMASGLGIVYVPKERVNVPANMGGASTDVAIVRLPLVNPLKDKAGIPVERPILND
REDEKGKAVMFVGYGVWGVGLNQGYGPESGTRRLYGRSKITSIFESDYGIGASYKPLGPSANWARVAAGDSGSAWWQIKD
TKPVIVATTNGGHARASTGARISKYANWIRSIYPDARFLSESRPQGCIISLRNGAKYCLEAGQKSDYSLPAWIYAHDVYV
QADSGVSVVLSDWDNLSYHRLAEFVGTVENDKLRGVKANNGETLDFSKPRSMEVKHSTRPLGCIVSLVSVDKYCLPAGER
SAYSLPAWIYAHDVVVQADAGIGVMLSDWDNLSYNRLAVFSDVVENENMKRVQAYDGQTLDFSKPRSMRVVQQ

Specific function: Virulence factor that contributes to intimate adherence of enterohemorrhagic E.coli (EHEC) O157:H7 to host cells. Is able to cleave the secreted human mucin 7 (MUC7) and the glycoprotein 340 (DMBT1/GP340). Also cleaves human C1 inhibitor (SERPING1), a reg

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Secreted via the etp type II secretion pathway [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase M66 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019503 [H]

Pfam domain/function: PF10462 Peptidase_M66 [H]

EC number: NA

Molecular weight: Translated: 51569; Mature: 51569

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILPLVALMLSSAVNAVVIDEDTFKRNGGDMRNVGASIRTANEKLQKHSTAKPWLSVG
CCHHHHHHHHHHHHCCCEEEECCCHHCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCHHHC
QLNGCTATWLGNKNGWAHILTAAHCVPYSATETPVRLTFSAWDGSVMASGLGIVYVPKER
CCCCEEEEEECCCCCCEEEEEHHHCCCCCCCCCCEEEEEECCCCCEECCCCCEEEECHHH
VNVPANMGGASTDVAIVRLPLVNPLKDKAGIPVERPILNDREDEKGKAVMFVGYGVWGVG
CCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEEEECEEEEC
LNQGYGPESGTRRLYGRSKITSIFESDYGIGASYKPLGPSANWARVAAGDSGSAWWQIKD
CCCCCCCCCCCHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEC
TKPVIVATTNGGHARASTGARISKYANWIRSIYPDARFLSESRPQGCIISLRNGAKYCLE
CCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCCHHHHH
AGQKSDYSLPAWIYAHDVYVQADSGVSVVLSDWDNLSYHRLAEFVGTVENDKLRGVKANN
CCCCCCCCCCEEEEEEEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCEECCC
GETLDFSKPRSMEVKHSTRPLGCIVSLVSVDKYCLPAGERSAYSLPAWIYAHDVVVQADA
CCEEECCCCCCEEECCCCCCHHHHHHHHHHCHHCCCCCCCCCCCCCEEEEEEEEEEEECC
GIGVMLSDWDNLSYNRLAVFSDVVENENMKRVQAYDGQTLDFSKPRSMRVVQQ
CCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCCEECCCCCCCCHHCCC
>Mature Secondary Structure
MRVILPLVALMLSSAVNAVVIDEDTFKRNGGDMRNVGASIRTANEKLQKHSTAKPWLSVG
CCHHHHHHHHHHHHCCCEEEECCCHHCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCHHHC
QLNGCTATWLGNKNGWAHILTAAHCVPYSATETPVRLTFSAWDGSVMASGLGIVYVPKER
CCCCEEEEEECCCCCCEEEEEHHHCCCCCCCCCCEEEEEECCCCCEECCCCCEEEECHHH
VNVPANMGGASTDVAIVRLPLVNPLKDKAGIPVERPILNDREDEKGKAVMFVGYGVWGVG
CCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEEEECEEEEC
LNQGYGPESGTRRLYGRSKITSIFESDYGIGASYKPLGPSANWARVAAGDSGSAWWQIKD
CCCCCCCCCCCHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEC
TKPVIVATTNGGHARASTGARISKYANWIRSIYPDARFLSESRPQGCIISLRNGAKYCLE
CCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCCHHHHH
AGQKSDYSLPAWIYAHDVYVQADSGVSVVLSDWDNLSYHRLAEFVGTVENDKLRGVKANN
CCCCCCCCCCEEEEEEEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCEECCC
GETLDFSKPRSMEVKHSTRPLGCIVSLVSVDKYCLPAGERSAYSLPAWIYAHDVVVQADA
CCEEECCCCCCEEECCCCCCHHHHHHHHHHCHHCCCCCCCCCCCCCEEEEEEEEEEEECC
GIGVMLSDWDNLSYNRLAVFSDVVENENMKRVQAYDGQTLDFSKPRSMRVVQQ
CCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCCEECCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9722640; 9628576; 12123444 [H]