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The map label for this gene is tniR [H]

Identifier: 94311939

GI number: 94311939

Start: 3263114

End: 3263728

Strand: Reverse

Name: tniR [H]

Synonym: Rmet_3007

Alternate gene names: 94311939

Gene position: 3263728-3263114 (Counterclockwise)

Preceding gene: 94311940

Following gene: 94311927

Centisome position: 83.09

GC content: 61.3

Gene sequence:

>615_bases
ATGCTGATTGGCTACATGCGGGTATCGAAAGCAGACGGCTCACAGGCCACCGACTTGCAGCGTGATGCACTGATCGCTGC
CGGCGTCGATCCGGCCCATCTCTACGAGGATCAAGCGTCGGGCAAACGCGAGGATCGTCCCGGCCTGTTGAGCTGCCTGA
AGGCGCTACGACCGGGGGATACGTTGATGGTGTGGAAACTTGACCGGCTCGGGCGCGATCTGCGGCATCTCATCAACACC
GTGCATGACCTGACCGGGCGAGGCATCGGTCTGAAAGTGTTGACTGGGCACGGTGCAGCCATTGACACCACGACGGCCGC
CGGCAAGTTGGTCTTCGGCATCTTCGCCGCGCTGGCCGAGTTCGAGCGCGAACTGATTACCGAGCGCACGATTGCAGGAT
TGGCTTCGGCGAGGGCGCGCGGTAGGAAGGGCGGCCGGCCCTTCAAGATGACTGCCGCCAAGTTGCGGCTGGCGATGGCC
GCTATGGGGCAGCCCGAAACCAAGGTCGGCGATCTATGTCAGGAACTGGGCATCACAAGACAGACCTTGTATCGGCACAT
TTCGCCGAAGGGCGAGTTGCGCCTTGATGGCGAGAAACTGCTCAGTCAAATTTGA

Upstream 100 bases:

>100_bases
ATGTCTTAGACAGAATGACGGGTTAAGTGACAAATTTTGACGACCAGAACTTTCCGGTGCACACTGTCACATAATCGAAC
GTATATGTGACAGGTGCAAG

Downstream 100 bases:

>100_bases
TGACAGGACAGCGGTTACTGTAGACGAATCGTGACGACTGGTTGAGTCAGTATGTCCTACCTGTTATCACTTGAACGCCA
ATAACAGAACGCCTCCGGCG

Product: serine-based site-specific recombinase activity (resolvase) (Tn6048)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MLIGYMRVSKADGSQATDLQRDALIAAGVDPAHLYEDQASGKREDRPGLLSCLKALRPGDTLMVWKLDRLGRDLRHLINT
VHDLTGRGIGLKVLTGHGAAIDTTTAAGKLVFGIFAALAEFERELITERTIAGLASARARGRKGGRPFKMTAAKLRLAMA
AMGQPETKVGDLCQELGITRQTLYRHISPKGELRLDGEKLLSQI

Sequences:

>Translated_204_residues
MLIGYMRVSKADGSQATDLQRDALIAAGVDPAHLYEDQASGKREDRPGLLSCLKALRPGDTLMVWKLDRLGRDLRHLINT
VHDLTGRGIGLKVLTGHGAAIDTTTAAGKLVFGIFAALAEFERELITERTIAGLASARARGRKGGRPFKMTAAKLRLAMA
AMGQPETKVGDLCQELGITRQTLYRHISPKGELRLDGEKLLSQI
>Mature_204_residues
MLIGYMRVSKADGSQATDLQRDALIAAGVDPAHLYEDQASGKREDRPGLLSCLKALRPGDTLMVWKLDRLGRDLRHLINT
VHDLTGRGIGLKVLTGHGAAIDTTTAAGKLVFGIFAALAEFERELITERTIAGLASARARGRKGGRPFKMTAAKLRLAMA
AMGQPETKVGDLCQELGITRQTLYRHISPKGELRLDGEKLLSQI

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=194, Percent_Identity=48.9690721649485, Blast_Score=156, Evalue=7e-40,
Organism=Escherichia coli, GI1787638, Length=188, Percent_Identity=34.0425531914894, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1787827, Length=188, Percent_Identity=34.5744680851064, Blast_Score=96, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 22112; Mature: 22112

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGYMRVSKADGSQATDLQRDALIAAGVDPAHLYEDQASGKREDRPGLLSCLKALRPGD
CEEECEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCC
TLMVWKLDRLGRDLRHLINTVHDLTGRGIGLKVLTGHGAAIDTTTAAGKLVFGIFAALAE
EEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHHHH
FERELITERTIAGLASARARGRKGGRPFKMTAAKLRLAMAAMGQPETKVGDLCQELGITR
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHH
QTLYRHISPKGELRLDGEKLLSQI
HHHHHHCCCCCCEEECHHHHHHCC
>Mature Secondary Structure
MLIGYMRVSKADGSQATDLQRDALIAAGVDPAHLYEDQASGKREDRPGLLSCLKALRPGD
CEEECEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCC
TLMVWKLDRLGRDLRHLINTVHDLTGRGIGLKVLTGHGAAIDTTTAAGKLVFGIFAALAE
EEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHHHH
FERELITERTIAGLASARARGRKGGRPFKMTAAKLRLAMAAMGQPETKVGDLCQELGITR
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHH
QTLYRHISPKGELRLDGEKLLSQI
HHHHHHCCCCCCEEECHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]