Click here to switch to the map view.

The map label for this gene is dut [H]

Identifier: 94311821

GI number: 94311821

Start: 3144487

End: 3144981

Strand: Direct

Name: dut [H]

Synonym: Rmet_2889

Alternate gene names: 94311821

Gene position: 3144487-3144981 (Clockwise)

Preceding gene: 94311820

Following gene: 94311826

Centisome position: 80.05

GC content: 63.84

Gene sequence:

>495_bases
ATGACTGTAGCCGCCAATCCGACCGTCGAAATCAAGGTGCTCGACGCCCGCCTGAATGACTGGGGCCTGCCCGCCTACCA
GAGCGACATGGCCGCAGCCATCGACCTGCACGCTTGCGTGGACGGCCCGGTCACGATCGAGGCCGGCACGCCTGCGCAAC
TGATTCCGGCCGGTATCGCGGTGCATATGGCCAATCCGTACATGTGCGCCACGATCGTGCCGCGCTCGGGTCTGGGCCAC
AAGAAGGGGCTCGTACTCGGCAATTCGATCGGTGTGATCGACGCGGATTACCAGGGGCAAATCATGGTCAGCGTGTGGAA
TCGCAACGCGCCCGGCACCGAACCGATCATGATCCAGCCCGGCGAGCGTATCGCCCAGATGATGTTCGTGCCGGTGCTGC
GTCCGGTGTTCACGACGGTGGAAGAATTCTCGGAAGATTCGACGCGTGGTGCCGGTGGCTTCGGGTCGACCGGCGTCGCG
CACGCAAAGGCGTAA

Upstream 100 bases:

>100_bases
AGGCCATCCGGCGCGGCGCGGTATGATTCGGCCTTTGGCCAGTCCGTGCTTTTGCCGGACTACCTAGCCCATCTTTCACG
CATTGCAGGAAACCGCTTCC

Downstream 100 bases:

>100_bases
AGCCAGAGCGGTACTCTCCCTCTCACGCTGTCCTGGAGAGGGAGAAAGATTTCAGCGCTGCTCGAAGATCAGCGCTACAC
CGTTGGCCTGCATCGCCGGG

Product: Deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MTVAANPTVEIKVLDARLNDWGLPAYQSDMAAAIDLHACVDGPVTIEAGTPAQLIPAGIAVHMANPYMCATIVPRSGLGH
KKGLVLGNSIGVIDADYQGQIMVSVWNRNAPGTEPIMIQPGERIAQMMFVPVLRPVFTTVEEFSEDSTRGAGGFGSTGVA
HAKA

Sequences:

>Translated_164_residues
MTVAANPTVEIKVLDARLNDWGLPAYQSDMAAAIDLHACVDGPVTIEAGTPAQLIPAGIAVHMANPYMCATIVPRSGLGH
KKGLVLGNSIGVIDADYQGQIMVSVWNRNAPGTEPIMIQPGERIAQMMFVPVLRPVFTTVEEFSEDSTRGAGGFGSTGVA
HAKA
>Mature_163_residues
TVAANPTVEIKVLDARLNDWGLPAYQSDMAAAIDLHACVDGPVTIEAGTPAQLIPAGIAVHMANPYMCATIVPRSGLGHK
KGLVLGNSIGVIDADYQGQIMVSVWNRNAPGTEPIMIQPGERIAQMMFVPVLRPVFTTVEEFSEDSTRGAGGFGSTGVAH
AKA

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI70906444, Length=145, Percent_Identity=33.7931034482759, Blast_Score=67, Evalue=5e-12,
Organism=Homo sapiens, GI4503423, Length=145, Percent_Identity=33.7931034482759, Blast_Score=67, Evalue=7e-12,
Organism=Homo sapiens, GI70906441, Length=145, Percent_Identity=35.1724137931034, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1790071, Length=151, Percent_Identity=49.6688741721854, Blast_Score=155, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI71988561, Length=158, Percent_Identity=32.9113924050633, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319729, Length=159, Percent_Identity=32.0754716981132, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 17212; Mature: 17081

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAANPTVEIKVLDARLNDWGLPAYQSDMAAAIDLHACVDGPVTIEAGTPAQLIPAGIA
CEECCCCEEEEEEEEECCCCCCCCCCCCCHHEEEEEEEECCCCEEEECCCCHHCCCCCEE
VHMANPYMCATIVPRSGLGHKKGLVLGNSIGVIDADYQGQIMVSVWNRNAPGTEPIMIQP
EEECCCEEEEEECCCCCCCCCCCEEECCCCCEEECCCCCEEEEEEECCCCCCCCCEEECC
GERIAQMMFVPVLRPVFTTVEEFSEDSTRGAGGFGSTGVAHAKA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCC
>Mature Secondary Structure 
TVAANPTVEIKVLDARLNDWGLPAYQSDMAAAIDLHACVDGPVTIEAGTPAQLIPAGIA
EECCCCEEEEEEEEECCCCCCCCCCCCCHHEEEEEEEECCCCEEEECCCCHHCCCCCEE
VHMANPYMCATIVPRSGLGHKKGLVLGNSIGVIDADYQGQIMVSVWNRNAPGTEPIMIQP
EEECCCEEEEEECCCCCCCCCCCEEECCCCCEEECCCCCEEEEEEECCCCCCCCCEEECC
GERIAQMMFVPVLRPVFTTVEEFSEDSTRGAGGFGSTGVAHAKA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA